Assessing Neuronal Excitability on a Fluorometric Imaging Plate Reader (FLIPR) Following a Defined Electrostimulation Paradigm.

FLIPR-based calcium assay enables the detection and characterization of neuronal excitability by using electrical field stimulation to evoke and record action potential-driven calcium transients in induced pluripotent stem cell (iPSC)-derived cortical forebrain neurons. Here we describe high throughput measurement of neuronal excitability with a defined electrostimulation paradigm in a 384-well plate format using FLIPR.