Cigarette smoke and nicotine effects on brain proinflammatory responses and behavioral and motor function in HIV-1 transgenic rats.

Cognitive impairment in HIV-1 infection is associated with the induction of chronic proinflammatory responses in the brains of infected individuals. The risk of HIV-related cognitive impairment is increased by cigarette smoking, which induces brain inflammation in rodent models. To better understand the role of smoking and the associated immune response on behavioral and motor function in HIV infection, wild-type F344 and HIV-1 transgenic (HIV1Tg) rats were exposed to either smoke from nicotine-containing (regular) cigarettes, smoke from nicotine-free cigarettes, or to nicotine alone. The animals were then tested using the rotarod test (RRT), the novel object recognition test (NORT), and the open field test (OFT). Subsequently, brain frontal cortex from the rats was analyzed for levels of TNF-α, IL-1, and IL-6. On the RRT, impairment was noted for F344 rats exposed to either nicotine-free cigarette smoke or nicotine alone and for F344 and HIV1Tg rats exposed to regular cigarette smoke. Effects from the exposures on the OFT were seen only for HIV1Tg rats, for which function was worse following exposure to regular cigarette smoke as compared to exposure to nicotine alone. Expression levels for all three cytokines were overall higher for HIV1Tg than for F344 rats. For HIV1Tg rats, TNF-α, IL-1, and IL-6 gene expression levels for all exposure groups were higher than for control rats. All F344 rat exposure groups also showed significantly increased TNF-α expression levels. However, for F344 rats, IL-1 expression levels were higher only for rats exposed to nicotine-free and nicotine-containing CS, and no increase in IL-6 gene expression was noted with any of the exposures as compared to controls. These studies, therefore, demonstrate that F344 and HIV1Tg rats show differential behavioral and immune effects from these exposures. These effects may potentially reflect differences in the responsiveness of the various brain regions in the two animal species as well as the result of direct toxicity mediated by the proinflammatory cytokines that are produced by HIV proteins and by other factors that are present in regular cigarette smoke.

The biology of race: searching for no overlap.

With the rise of molecular genetics and the cornucopia of techniques it provides, a number of biomedical researchers in both the public and private sectors have turned to the human genome to search for variations among the world's populations, with the purpose of tracing human evolution and migration patterns and predicting genetic disorders. The human genome also offers insight into an individual's genealogy, which has never before been charted with such precision. Whereas 18th- and 19th-century scholars fetishized external characteristics in order to classify humans,more recent scholars have turned to the internal sequence of the genome. But how does one define populations? Should they be based on race? Can one speak of race at the molecular level? This essay explores the history of the biology of race with a view to compare and contrast modern molecular biological studies with the more pernicious actions of early-20th-century eugenicists. The key linking the two practices is the search for biological entities that do not overlap among the races. A critical difference is that modern studies use molecular biology to include previously excluded populations in the treatment of certain diseases. While the intentions are quite different, a number of scholars feel that genetic essentialism might be the end result.

[In process.] / Die Genealogie eines Gens: Patente, HIV/AIDS und Rasse.

The story, which unfolds here, is a cultural history of science, one that closely analyzes the content of science. My story deals with an object, a gene. I use the CCR5 gene as a heuristic tool in order to probe the boundaries between science and society. Three important themes are discussed in this essay: genes as commodities (intellectual property and gene patents); alleles, natural selection, and the resistance to disease; and race and genomics. This is in part a story about neoliberalism, laissez-faire goverenments, free and open markets, the increase of privatization, and biotechnology. Many claim that the United States Patent and Trademake Office's (henceforth, USPTO) leniency in granting gene patenting led to the growth of biotechnology. I maintain the opposite: the growth of biotechnology led to decision to patent genes. My story is one of the present, a genealogy to borrow FOUCAULT'S and NIETZsCHE's terminology. How has it come about that genes are patentable entities, and that human classificatory schemes are usually based on race, although there are an infinite number of possibilities to characterize human variation? There are always alternatives, and historians are obliged to present those alternatives and explain why they were never chosen. I also use the concept of genealogy in the classical biological sense, i.e. to trace the passing of alleles from one generation to another. While this essay is similar to earlier studies dealing with the biography of objects, particularly scientific objects, the history told here is not a biography of the CCR5 gene, as that story is still ongoing. Rather, this essay concentrates upon a twenty-year period of the gene's life from the mid-1990s to the present. I am interested in understanding how it is we have reached the point we have today with respect to the relationship between science and society, and I use the CCR5 gene as a vehicle for that analysis.

Music and physics: a cultural, interdisciplinary history.

This essay investigates the triangular exchange among physicists, musicians, and instrument makers in nineteenth-century Germany by proffering a material, cultural, and interdisciplinary history. It does so by analyzing four concrete examples of such exchanges: the relationship between musical automata and virtuosi, the reed pipe as an object of music and scientific measurement, the history of standardizing performance pitch, and the attempts to measure musical virtuosity. The goal of the essay is to suggest ways in which interdisciplinary cultural histories, which take the scientific content seriously, can be an improvement upon purely disciplinary histories.