RESUMO
The influence of pefloxacin on polymorphonuclear leucocytes (PMN) and alveolar macrophages (AM) phagocytosis and microbicidal activity was studied. Phagocytes were collected from Sprague-Dawley rats, mixed with pefloxacin at different concentrations (1, 10, 100 mg/l) and a yeast phagocytosis assay was performed. Three parameters were measured, the phagocytic capacity (number of live and dead yeast/100 cells), the phagocytic activity (number of cells containing 2 or more yeast/100 cells) and the microbicidal activity or % killed (Formula: see text) Both low and high concentrations of pefloxacin increased significantly the phagocytic capacity and activity of AM and PMN. The increase of the AM phagocytic capacity and activity seen with increasing levels of extracellular pefloxacin was not statistically significant. Pefloxacin showed no adverse effect on the microbicidal activity of AM and PMN.
Assuntos
Anti-Infecciosos/farmacologia , Macrófagos/efeitos dos fármacos , Neutrófilos/efeitos dos fármacos , Norfloxacino/análogos & derivados , Fagocitose/efeitos dos fármacos , Animais , Macrófagos/fisiologia , Neutrófilos/fisiologia , Norfloxacino/farmacologia , Pefloxacina , Ratos , Ratos EndogâmicosRESUMO
Spiramycin concentrations in lung tissue were studied in patients undergoing pulmonary surgical procedures. The first group of six patients received 500 mg spiramycin iv 16 h before surgery and 500 mg at anaesthetic induction (total 1 g). The second group of six patients received three doses of 500 mg spiramycin iv 24, 16 and 8 h before surgery, and 500 mg at anaesthetic induction (total 2 g). Samples were taken from lung tissue, pleura, fat tissue and muscle. In group 1, the mean lung tissue concentration of spiramycin was 1.15 +/- 0.14 mg/kg and 7.99 +/- 2.02 mg/kg in group 2 (P less than 0.02). The differences in concentration in pleura, fat tissue and muscle samples between treatment groups 1 and 2 were not statistically significant.