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The purpose of this study is to explore physiological and psychological changes immediately after a short-term visit to urban parks in an uncontrolled condition. Sixty park visitors completed a short questionnaire evaluating their subjective well-being (SWB) and donated a saliva sample immediately before and after their park visit. In addition, participants wore a pedometer to track their physical activity level during the park visit. Results indicated the levels of all biomarkers (cortisol, dehydroepiandrosterone [DHEA], and cortisol/DHEA ratio) decreased while SWB scores increased after the park visit. The duration of park visit was identified as a key variable significantly associated with cortisol reduction, and changes in SWB scores after the park visit were significantly associated with changes in cortisol level. A park visit duration of 21.8 min has the predictive ability to discriminate park visitors who exhibit reductions in cortisol levels after the park visit from those who do not.
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Hidrocortisona , Parques Recreativos , Humanos , Inquéritos e Questionários , Saliva , DesidroepiandrosteronaRESUMO
IMPORTANCE: Low social competence is one of the most complex and resistant challenges faced by adolescents with autism spectrum disorder (ASD). Occupational therapy practitioners have recognized the potential benefits of using the arts, including training in magic tricks, as a therapeutic medium to improve and enhance clients' psychosocial well-being. OBJECTIVE: To describe the efficacy of a virtual magic trick training program (MTTP) to enhance social skills and self-esteem in adolescents with ASD. DESIGN: Pragmatic, nonrandomized, wait-list controlled trial with 1-mo follow-up. SETTING: Participants' homes. PARTICIPANTS: Seventeen adolescents (ages 9-15 yr) with ASD participated in the MTTP, 9 in the first cohort and 8 in the second (wait-list control) cohort. INTERVENTION: Participants received magic trick training from occupational therapy students in pairs via videoconferencing in 45-min sessions, 3 days/wk, for 3 wk. OUTCOMES AND MEASURES: Participants completed the Rosenberg Self-Esteem Scale and the Social Skills Improvement System before and after participating in the program. RESULTS: Between-cohort effect sizes (Cohen's d) were 0.58 for social skills and 0.66 for self-esteem, considered moderate effects. Combining the two cohorts revealed significant improvements in social skills and self-esteem, with gains maintained at 1-mo follow-up. CONCLUSIONS AND RELEVANCE: The results of this study support our hypothesis that adolescents with ASD who participate in the 3-wk virtual MTTP can experience enhanced social skills and self-esteem. What This Article Adds: Learning magic tricks through individual coaching from occupational therapy students in a virtual environment can enhance the social skills and self-esteem of adolescents with ASD.
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Transtorno do Espectro Autista , Tutoria , Adolescente , Humanos , Criança , Habilidades Sociais , Estudantes , AprendizagemRESUMO
This study seeks to examine the effect of the characteristics of park users, park characteristics, and the neighborhood environment on park visits and the resultant perceived health status of residents living in Alabama, US. One hundred and eighty-nine participants, representing residents from 49 counties in Alabama, were recruited via a random-digit dial method to complete a telephone survey related to park visits. Data collection spanned 11 months. Results from the path analysis indicated a few significant direct effects: park location and body mass index (BMI) were the only two factors significantly associated with park visits, with race being marginally significant; and work status was significantly associated with perceived health. Our findings suggest park proximity, and high BMI are related to park visits and work status is related to perceived health among Alabamians. It is recommended that more neighborhood parks are needed for promoting Alabamians to visit them more frequently.
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Nível de Saúde , Características de Residência , Alabama , Estudos Transversais , Humanos , Parques Recreativos , RecreaçãoRESUMO
This study aimed to explore the amenable factors contributing to the improvement in subjective well-being (SWB) immediately after a short-term visit to an urban park in an uncontrolled condition. Ninety-four park visitors from three urban parks completed a short questionnaire evaluating SWB (with two components: affect and life satisfaction) immediately before and after their park visit. In addition, their level of physical activity was tracked by wearing an accelerometer during the park visit. Results indicated a significant improvement in SWB, affect, and life satisfaction scores of park visitor participants from before and after their visit. Duration of park visit was bivariately associated with SWB scores, and independently associated with the improvement in life satisfaction scores, controlling for parks and age, after the visit; a 20.5-min park visit predicted the highest overall accuracy (64%) improvement in life satisfaction. It is recommended that design of the park space should attract visitors to stay for at least 20 min in the park.
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Exercício Físico , Saúde Mental/estatística & dados numéricos , Parques Recreativos/estatística & dados numéricos , Recreação/psicologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Alabama , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto JovemRESUMO
This single-group open trial was designed to evaluate the effectiveness of a two-week magic camp as a means of hand-arm motor skills training to improve upper limb motor function (unilateral and bilateral) in children with hemiparesis. Seven children with hemiparesis participated in a magic camp program which met 3 days a week, 4 hours each day, for two consecutive weeks for a total of 24 hours. Participants completed three assessments at the beginning of the camp, post-camp, and at a three-month follow-up: the Jebsen Hand Function Test (JHFT), Children's Hand Experience Questionnaire (CHEQ), and a box opening task that required coordination of both upper limbs. A Wilcoxon signed-rank test revealed significant improvement in JHFT composite scores of the affected limb at post-camp (p = .04) and three-month follow-up (p = .04). In addition, a significant improvement in the number of activities performed using two hands from baseline to three-month follow-up was observed (p = .03). This pattern of improvement was also observed in the speed of completion for the box opening task. The improvement in motor function seems related to the participants' continuing performing daily activities with the affected hand and two hands after the magic camp.
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Paralisia Cerebral/fisiopatologia , Paralisia Cerebral/reabilitação , Magia , Paresia/fisiopatologia , Paresia/reabilitação , Recreação , Adolescente , Criança , Cuidado da Criança , Avaliação da Deficiência , Feminino , Humanos , Masculino , Projetos PilotoRESUMO
Transposition is a tendency for organisms to generalize relationships between stimuli in situations where training does not objectively reward relationships over absolute, static associations. Transposition has most commonly been explained as either conceptual understanding of relationships (Köhler, 1938) as nonconceptual effects of neural memory gradients (as in Spence's stimulus discrimination theory, 1937 ). Most behavioral evidence can be explained by the gradient account, but a key finding unexplained by gradients is intermediate transposition, where a central (of three) stimulus, "relationally correct response," is generalized from training to test. Here, we introduce a dynamic neural field (DNF) model that captures intermediate transposition effects while using neural mechanisms closely resembling those of Spence's original proposal. The DNF model operates on dynamic rather than linear neural relationships, but it still functions by way of gradient interactions, and it does not invoke relational conceptual understanding in order to explain transposition behaviors. In addition to intermediate transposition, the DNF model also replicates the predictions of stimulus discrimination theory with respect to basic two-stimulus transposition. Effects of wider test item spacing were additionally captured. Overall, the DNF model captures a wider range of effects in transposition than stimulus discrimination theory, uses more fully specified neural mechanics, and integrates transposition into a wider modeling effort across cognitive tasks and phenomena. At the same time, the model features a similar low-level focus and emphasis on gradient interactions as Spence's, serving as a conceptual continuation and updating of Spence's work in the field of transposition.
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Redes Neurais de Computação , Animais , Simulação por Computador , Discriminação Psicológica/fisiologia , Generalização Psicológica/fisiologia , Humanos , Modelos NeurológicosRESUMO
This study investigated self-reported prevalence of and factors affecting clinicians' use of standardized assessments when evaluating clients for power mobility devices (PMDs), and explored assessments clinicians typically use when carrying out PMD evaluation. An e-mail survey was sent to assistive technology professionals listed in the Rehabilitation Engineering and Assistive Technology Society of North America directory. Three hundred fifty-four respondents, qualified to conduct formal power mobility skills assessments, completed the online survey. Of those, 122 (34.5%) respondents reported that they were aware of the presence of standardized performance-based power mobility skills assessments, but only 28 (7.9%) used these assessments in their practice. Multivariate analysis revealed that the odds of the respondents who use the standardized assessments were 18 times higher for those who were aware of the presence of these assessments than those who were not (adjusted odds ratio [OR] OR = 17.85, P < 0.0001). The odds of using the standardized assessment for respondents who did not identify themselves as occupational or physical therapists were five times higher than those who were therapists (adjusted OR = 0.20, P < 0.0001). This survey revealed that the assistive technology practitioners who recommend PMDs mainly use non-standardized mobility skills assessments.
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Fisioterapeutas/estatística & dados numéricos , Análise e Desempenho de Tarefas , Cadeiras de Rodas/estatística & dados numéricos , Cadeiras de Rodas/normas , Estudos Transversais , Humanos , Limitação da Mobilidade , Avaliação da Tecnologia Biomédica/normasRESUMO
Infection of the mammalian host by schistosome larvae occurs via the skin, although nothing is known about the development of immune responses to multiple exposures of schistosome larvae, and/or their excretory/secretory (E/S) products. Here, we show that multiple (4x) exposures, prior to the onset of egg laying by adult worms, modulate the skin immune response and induce CD4(+) cell hypo-responsiveness in the draining lymph node, and even modulate the formation of hepatic egg-induced granulomas. Compared to mice exposed to a single infection (1x), dermal cells from multiply infected mice (4x), were less able to support lymph node cell proliferation. Analysis of dermal cells showed that the most abundant in 4x mice were eosinophils (F4/80(+)MHC-II(-)), but they did not impact the ability of antigen presenting cells (APC) to support lymphocyte proliferation to parasite antigen in vitro. However, two other cell populations from the dermal site of infection appear to have a critical role. The first comprises arginase-1(+), Ym-1(+) alternatively activated macrophage-like cells, and the second are functionally compromised MHC-II(hi) cells. Through the administration of exogenous IL-12 to multiply infected mice, we show that these suppressive myeloid cell phenotypes form as a consequence of events in the skin, most notably an enrichment of IL-4 and IL-13, likely resulting from an influx of RELMα-expressing eosinophils. We further illustrate that the development of these suppressive dermal cells is dependent upon IL-4Rα signalling. The development of immune hypo-responsiveness to schistosome larvae and their effect on the subsequent response to the immunopathogenic egg is important in appreciating how immune responses to helminth infections are modulated by repeated exposure to the infective early stages of development.
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Derme/imunologia , Células Mieloides/imunologia , Schistosoma mansoni/imunologia , Esquistossomose mansoni/imunologia , Dermatopatias Parasitárias/imunologia , Células Th2/imunologia , Animais , Células Apresentadoras de Antígenos/imunologia , Células Apresentadoras de Antígenos/patologia , Proliferação de Células , Citocinas/genética , Citocinas/imunologia , Derme/parasitologia , Derme/patologia , Eosinófilos/imunologia , Eosinófilos/patologia , Feminino , Linfonodos/imunologia , Linfonodos/patologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Knockout , Células Mieloides/patologia , Receptores de Superfície Celular/genética , Receptores de Superfície Celular/imunologia , Esquistossomose mansoni/genética , Transdução de Sinais/genética , Transdução de Sinais/imunologia , Dermatopatias Parasitárias/genética , Dermatopatias Parasitárias/parasitologiaRESUMO
Background: Classroom lighting, usually bright fluorescent light, can significantly influence the learning environment and emotions of students. Objective: To assess the emotional impact of classroom lighting on students during an academic year. Methods: This study used an ABAB withdrawal research design in the following manner: in phase A, the baseline condition, classroom lighting was provided by conventional overhead white fluorescent classroom lights; in phase B, the intervention condition, the conventional overhead white fluorescent classroom lights were covered with fabric filters (thin, translucent, creamy-colored plastic sheets) that were attached to the lighting fixture frame with magnetic discs. The filters produced softer light in the classroom than the fluorescent lights. Each phase lasted for at least 2 weeks. During each phase, students rated 18 pairs of words from the Mehrabian and Russell pleasure, arousal, and dominance semantic differential scale at least four times to assess the emotional impact of the lighting conditions. Results: For all three emotional behaviors, the mean score of the filtered fluorescent light phase was significantly greater than the mean score of the baseline unfiltered fluorescent lighting phase, indicating more positive emotional responses. Students also noted they experienced fewer headaches and found it easier to see the whiteboard at the front of the classroom when the light filters were in place. Conclusion: The light filters exerted a positive impact on the students' emotions. Students preferred the filtered lighting to fluorescent lighting. This study supports the installation of filters over fluorescent lights in a college classroom.
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The Transition to Wellness Program is an occupational therapy (OT) based program that was developed in response to the COVID-19 pandemic. For OT students, fieldwork is a required element of their training. In March 2020, these were being halted due to lockdowns and the risk to students in healthcare settings. Facing significant delays to graduation for 60 students, including some unable to be placed into healthcare settings due to underlying conditions, the development of a telehealth program was conceived to address this, while still meeting the accreditation clinical/health/wellness standards for OT fieldwork. A group of students, with faculty oversight, developed the theoretical basis, tools, policies and procedures for the program. An incremental launch allowed testing and refinement of the program to meet the educational needs of the students and the wellness needs of the clients. At its launch, the program supported 10 of that cohorts' 60 students to continue to meet the required academic standards and progress through their fieldwork towards graduation. Each student got to experience the "telehealth" model in providing OT wellness interventions to individuals in the local community. Future work will focus on continuing to broaden the reach of the program and measure the outcomes.
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In their 2007b Psychological Review paper, Xu and Tenenbaum found that early word learning follows the classic logic of the "suspicious coincidence effect:" when presented with a novel name ('fep') and three identical exemplars (three Labradors), word learners generalized novel names more narrowly than when presented with a single exemplar (one Labrador). Xu and Tenenbaum predicted the suspicious coincidence effect based on a Bayesian model of word learning and demonstrated that no other theory captured this effect. Recent empirical studies have revealed, however, that the effect is influenced by factors seemingly outside the purview of the Bayesian account. A process-based perspective correctly predicted that when exemplars are shown sequentially, the effect is eliminated or reversed (Spencer, Perone, Smith, & Samuelson, 2011). Here, we present a new, formal account of the suspicious coincidence effect using a generalization of a Dynamic Neural Field (DNF) model of word learning. The DNF model captures both the original finding and its reversal with sequential presentation. We compare the DNF model's performance with that of a more flexible version of the Bayesian model that allows both strong and weak sampling assumptions. Model comparison results show that the dynamic field account provides a better fit to the empirical data. We discuss the implications of the DNF model with respect to broader contrasts between Bayesian and process-level models.
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Aprendizagem , Aprendizagem Verbal , Teorema de Bayes , Pesquisa Empírica , Generalização Psicológica , Humanos , Modelos PsicológicosRESUMO
BACKGROUND: Children with attention-deficit/hyperactivity disorder (ADHD) have been reported to experience low self-esteem. Magic, a performing art, when used therapeutically, has been shown to enhance self-esteem in children with neurodevelopmental disorders. However, there is a paucity of evaluation studies using this intervention applied to children with ADHD. PURPOSE: This single-group study aims to describe the effectiveness of a virtual summer magic camp program to enhance self-esteem in children and adolescents with ADHD. METHOD: Six children with ADHD, age 8.6-14.3 years, who participated in a virtual magic camp program for children with disabilities, were included in this study. The camp met 3 days a week, about 45 min to an hour each session, over four consecutive weeks for a total of 9 to 12 hours. Participants completed the assessments before the beginning of the camp and post-camp. In addition, participants and their parents were individually interviewed to explore their camp experience at the post-camp data point. RESULTS: A Wilcoxon signed-rank test indicated that the median ranks of the self-esteem scores at post-magic camp, median=21.5, were significantly higher than the median ranks of the self-esteem scores at baseline, median=19.5, with z=2.23, p=.026. The effect size of the virtual magic camp on self-esteem was .64, which is considered moderate. Findings were validated by the participants describing gains in self-esteem after participation in the magic camp and by the parents' statements regarding the positive impact on their child's psychological well-being. DISCUSSION: This study supported the hypothesis that children with ADHD who participated in a four-week virtual magic camp experienced enhanced self-esteem. Future studies should investigate the holistic impact of magic camps on children and adolescents with ADHD and include measures that tap into other psychosocial attributes such as social functioning, social skills, and self-efficacy.
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Previous studies have examined the quantitative results of using an organized approach of teaching and learning magic tricks-a therapeutic magic camp-but set aside the qualitative aspect. The purpose of this qualitative study is to explore the interpretation of the lived experience of children with hemiparesis after they have participated in a therapeutic magic camp. Six children, aged between 11 and 14 years old, with hemiparesis who completed the magic camp program 3 months prior were invited to participate in an in-person individual semistructured interview. The interviews were audio-recorded and transcribed verbatim for content analysis. Results from the initial qualitative analysis yielded categories of enjoyment, positive social and learning experience from participation in the camp, increased confidence, and self-motivation to use the affected upper limb. These categories were grouped into two themes: "having fun with others while learning" and "helping my arm perform." Having fun with others while learning was an amalgamation of fun, and positive social and learning experience. Helping my arm perform reflected a sense of increased competence and self-belief, and a willingness and motivation of the children to attempt challenging daily activities. The lived experiences of children with hemiplegia who participated in an organized delivery of learning to perform magic tricks yield-ed positive outcomes. These themes are consistent with the limited literature that is available suggesting that further research is needed to confirm the efficacy of the therapeutic magic camp intervention.
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RATIONALE: Dithiothreitol (DTT) is commonly used to liquefy induced sputum samples before assessment of cytology, but causes reduction of disulfide bonds and denaturation of proteins. OBJECTIVES: To process sputum supernatants containing DTT to enable quantification of cytokines and chemokines. METHODS: A standard solution of 22 pooled chemokines and cytokines was incubated with DTT at the concentrations used during sputum liquefaction and then dialyzed under 20 different denaturant and redox conditions. MEASUREMENTS AND MAIN RESULTS: After incubation of the standard solution with DTT there was loss of detectable protein mediators on immunoassay, but optimized dialysis permitted recovery of chemokines to 96 +/- 4% and cytokines to 91 +/- 6%. Optimized dialysis of DTT supernatants from subjects with asthma covering a range of severities (n = 35) was performed in the presence of a cocktail of protease inhibitors and demonstrated significantly elevated levels of the chemokine CXCL10 (IFN-gamma-inducible protein-10), CXCL8 (IL-8), and CCL3 (macrophage inflammatory protein-1alpha); with lower but significantly elevated levels of CCL2 (monocyte chemotactic protein-1), CCL11 (eotaxin), and CCL5 (regulated on activation, normal T-cell expressed and secreted) in severe asthma. In sputum from subjects with severe asthma there were also significantly elevated levels of IL-4, IL-5, IL-13, tumor necrosis factor-alpha, IL-6, granulocyte-macrophage colony-stimulating factor, and IL-12(p40). CONCLUSIONS: The technique of optimized dialysis and protease inhibition of sputum DTT supernatants aids the detection of chemokines and cytokines. The detection of elevated levels of particular sputum chemokines and cytokines in individual patients may provide a rationale for specific therapies.
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Asma/imunologia , Quimiocinas/análise , Citocinas/análise , Diálise/métodos , Ditiotreitol , Inibidores de Proteases , Escarro/química , Adulto , Idoso , Estudos de Casos e Controles , Contagem de Células , Estudos Transversais , Diálise/instrumentação , Feminino , Volume Expiratório Forçado , Humanos , Masculino , Pessoa de Meia-Idade , Soroalbumina Bovina , Índice de Gravidade de Doença , Manejo de Espécimes/métodosRESUMO
PUPROSE: This study is to describe the development and validation of the Hocus Focus Magic Performance Evaluation Scale (HFMPES) which is used to evaluate the competency of health professions personnel in delivering magic tricks as a therapeutic modality. METHODS: A two-phase validation process was used. Phase I content validation involved 16 magician judges who independently rated the relevance of each of the five items in the HFMPES and established the veracity of its content. Phase II was to evaluate the psychometric properties of the HFMPES. This process involved 2 magicians using the HFMPES to independently evaluate 73 occupational therapy graduate students demonstrating three magic tricks. RESULTS: The HFMPES achieved an excellent scale-content validity index of .99. Exploratory factor analysis of the HFMPES scores revealed one distinct factor with alpha coefficients ≥.8 across the three magic tricks. The construct validity of the HFMPES scores was further supported by the evidence from the known-groups analysis in which Mann-Whitney U test showed significant difference in HFMPES scores between participants with different levels of experience in delivering the three magic tricks. The inter-rater reliability coefficients were ≥.75 across the three magic tricks, which indicated that evaluation of the competency of health professions personnel in delivering the three magic tricks can be evaluated precisely. CONCLUSION: Preliminary evidence supported the content and construct validity of the HFMPES with good internal consistency and inter-rate reliabilities in evaluating health professions personnel's competency in delivering magic tricks.
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Pessoal de Saúde , Magia/psicologia , Terapia Ocupacional , Psicometria/estatística & dados numéricos , Adulto , Estudos Transversais , Educação de Pós-Graduação , Feminino , Humanos , Masculino , Reprodutibilidade dos Testes , Inquéritos e Questionários , Estados UnidosRESUMO
BACKGROUND: Rhinovirus infection is a major cause of asthma exacerbations. While rhinovirus infection is known to generate kinins in the upper respiratory track, little is known about the effect of rhinovirus on kinin generation in the lower airway. We previously identified human tissue kallikrein (hTK) as the principal lung kininogenase during allergic airway inflammation. In this report we investigate the effect of experimental rhinovirus infection on hTK activity in the airways of atopic subjects with and without asthma. METHODS: Eight atopic subjects, 4 with asthma, underwent bronchoscopy with lavage. At least 1 month later, subjects were inoculated with rhinovirus, then underwent repeat bronchoscopy with lavage 4 and 18 days later. hTK mRNA was measured in nasal scrape samples by quantitative real-time PCR. hTK activity (chromogenic substrate assay) and IL-8 levels (ELISA) were assessed in the bronchoalveolar lavage fluid. RESULTS: At day 4 after rhinovirus inoculation, nasal hTK mRNA was modestly increased in both the rhinitis (1.7-fold) and asthmatic (2.1-fold) groups. A doubling or greater increase in hTK activity after rhinovirus infection was observed in all 4 asthmatic subjects (mean 19-fold increase) but only in 1 of 4 atopic subjects without asthma (mean 2-fold increase). Rhinovirus infection also increased the IL-8 protein level in bronchoalveolar lavage fluid, which correlated with hTK activity (R = 0.82). CONCLUSION: Experimental rhinovirus infection in allergic asthmatic subjects is accompanied by increased lower airway hTK activation, which parallels the appearance of IL-8. Rhinovirus-induced hTK activation may contribute to airway inflammation and asthmatic exacerbations.
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Hipersensibilidade/enzimologia , Infecções por Picornaviridae/enzimologia , Rhinovirus , Calicreínas Teciduais/metabolismo , Adulto , Ativação Enzimática , Feminino , Humanos , Interleucina-8/biossíntese , Masculino , Infecções por Picornaviridae/imunologia , RNA Mensageiro/análise , Calicreínas Teciduais/genéticaRESUMO
Experts around the world support the integration of health promotion and wellness (HPW) services into traditional health care services. If successfully executed, the addition of HPW services would reduce rates of death and disability and significantly reduce health care costs. While all health care providers should be engaged in providing HPW services, many believe that physical therapists (PTs) and occupational therapists (OTs) are uniquely positioned to provide these services. However, research suggests that clinicians in both fields may fall short in doing so. Likewise, research indicates that entry-level educational programs inadequately prepare PT and OT students to be HPW practitioners. The overall purpose of this paper is to provide recommendations to educators for preparing PT and OT students and clinicians to better meet the HPW needs of the clients and patients they serve.
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Promoção da Saúde , Terapia Ocupacional/educação , Especialidade de Fisioterapia/educação , Currículo , Humanos , Estados UnidosRESUMO
Infectious cercariae of Schistosoma mansoni gain entry to the mammalian host through the skin where they induce a transient inflammatory influx of mononuclear cells. Some of these cells have antigen-presenting cell function (MHCII+) and have been reported to migrate to the skin-draining lymph nodes (sdLN) where they have the potential to prime CD4+ cells of the acquired immune response. Here, in mice exposed to vaccinating radiation-attenuated schistosome larvae, which induce high levels of protective immunity to challenge infection, we describe the parasite-induced migration of Langerhans cells (LCs) from the epidermal site of immunisation to the sdLN using a specific monoclonal antibody that recognises langerin (CD207). CD207+ cells with dendritic morphology were abundant in the epidermis at all times and their migration into the dermis was detected soon after vaccination. All CD207+ LCs were MHCII+ but not all MHCII+ cells in the skin were CD207+. LCs migrated from the dermis in enhanced numbers after vaccination, as detected in dermal exudate populations recovered after in vitro culture of skin biopsies. Elevated numbers of CD207+ LCs were also detected in the sdLN from 24h to 4 days after vaccination. However, compared with other dermal-derived antigen-presenting cells that were CD207-MHCII+ or CD207-CD11c+, the relative numbers of CD207+ cells in the dermal exudate population and in the sdLN were very small. Furthermore, the migration of CD207+ cells after exposure to 'protective' radiation-attenuated, compared with 'non-protective' normal cercariae, was similar in terms of numbers and kinetics. Together, these studies suggest that CD207+ LCs are only a minor component of the antigen-presenting cell population that migrates from the epidermis and they are unlikely to be important in the priming of protective CD4+ cells in the sdLN.
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Antígenos de Superfície/imunologia , Células Epidérmicas , Células de Langerhans/imunologia , Lectinas Tipo C/imunologia , Linfonodos/imunologia , Lectinas de Ligação a Manose/imunologia , Schistosoma mansoni/imunologia , Esquistossomose mansoni/imunologia , Animais , Anticorpos Monoclonais/farmacologia , Derme/citologia , Feminino , Imunofenotipagem , Camundongos , Camundongos Endogâmicos C57BLRESUMO
Little evidence exists to guide the management of children with difficult asthma. The aim of this study was to determine whether children with difficult asthma, associated with sputum eosinophilia, are more likely to benefit from further treatment with high-dose systemic corticosteroids, compared to those without sputum eosinophilia. Induced sputum was obtained from 20 children aged between 8 and 15 years with difficult asthma before and after a systemic corticosteroid trial (prednisolone 40 mg/day for 14 days or a single 80 mg dose of intramuscular triamcinolone). Subjects were defined as "eosinophilic" if the baseline sputum eosinophil percentage was > or = 2.5% or "non-eosinophilic" if < 2.5%. Clinical response to the corticosteroid trial was assessed using spirometry and clinical data and defined by an increase in pre-bronchodilator forced expiratory volume in 1 sec (FEV1) > 9% predicted and/or an overall subjective improvement. Seventeen children had evidence of satisfactory adherence to the systemic corticosteroid treatment; eight of these were "eosinophilic" and nine were "non-eosinophilic" subjects. Following the trial there was a similar clinical improvement in both groups, with FEV1 increasing in both the "eosinophilic" and "non-eosinophilic" groups (median change in FEV1 16 [range 5-54]% vs. 12.5 [1-29]% predicted). Sputum eosinophils fell in the "eosinophilic" group (median 17.5 [range 3-37]% vs. 0 [0-23]%, P = 0.054), with no change in the "non-eosinophilic" group (0 [0-2]% vs. 0 [0-1]%, P = 0.12). Sputum neutrophils did not change in either the "eosinophilic" (22.5 [5-50]% vs. 25 [0-91]%) or the "non-eosinophilic" group (27.5 [0-96] vs. 44 [9-96]%). In conclusion children with difficult asthma may benefit clinically from high-dose systemic corticosteroids even in the absence of sputum eosinophilia.
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Asma/tratamento farmacológico , Asma/imunologia , Eosinofilia/patologia , Glucocorticoides/uso terapêutico , Prednisolona/uso terapêutico , Escarro/citologia , Triancinolona/uso terapêutico , Adolescente , Criança , Feminino , Humanos , Masculino , Valor Preditivo dos TestesRESUMO
BACKGROUND: To identify physical measures that predict maximal handgrip strength (MHGS) and provide evidence for identifying lack of sincerity of effort when assessing upper extremity weakness. OBJECTIVE: This study investigated anthropometric measurements associated with MHGS of healthy young adults. METHODS: A convenience sample of 150 healthy adults ages 19 to 34 years old completed the MHGS assessment, which was measured using a Jamar dynamometer according to the protocol of the American Society of Hand Therapists, for both dominant and non-dominant hands. Several anthropometric data were collected, which included height, body weight, forearm length, forearm circumference, hand length, and hand width. RESULTS: Multivariable linear regression analysis indicated gender and hand width were uniquely and significantly associated with participants' MHGS for dominant and non-dominant hand and accounted for more than 60% of the variance, with R2â=â0.60, Pâ<â0.001 for the dominant hand model and R2â=â0.64, Pâ<â0.001 for the non-dominant hand model. CONCLUSIONS: Among the forearm and hand anthropometric measures, hand width is the best predictor of MHGS in both the non-dominant and dominant hands for healthy young adults.