Tracing conflict-induced cognitive-control adjustments over time using aperiodic EEG activity.

Cognitive-control theories assume that the experience of response conflict can trigger control adjustments. However, while some approaches focus on adjustments that impact the selection of the present response (in trial N), other approaches focus on adjustments in the next upcoming trial (N + 1). We aimed to trace control adjustments over time by quantifying cortical noise by means of the fitting oscillations and one over f algorithm, a measure of aperiodic activity. As predicted, conflict trials increased the aperiodic exponent in a large sample of 171 healthy adults, thus indicating noise reduction. While this adjustment was visible in trial N already, it did not affect response selection before the next trial. This suggests that control adjustments do not affect ongoing response-selection processes but prepare the system for tighter control in the next trial. We interpret the findings in terms of a conflict-induced switch from metacontrol flexibility to metacontrol persistence, accompanied or even implemented by a reduction of cortical noise.

Melatonin alleviates cadmium toxicity and abiotic stress by promoting glandular trichome development and antioxidant capacity in Nicotiana tabacum.

Melatonin is a well-known signaling molecule that mediates a range of physiological activities and various stress reactions in plants. We comprehensively tested the effect of melatonin on the development of root hairs and glandular trichomes and found that melatonin pretreatment of tobacco seeds significantly increased the length of root hairs. Furthermore, melatonin-treated tobacco exhibited significantly higher density of trichomes and larger glandular heads on long-stalk glandular trichomes than untreated plants, which resulted in enhanced secretion in glandular trichomes. Exogenous melatonin enhanced the aphid resistance of plants by facilitating the accumulation of cembranoids in the glandular trichomes and alleviated cadmium toxicity by increasing the Cd-exudation capacity of long glandular trichomes. Metabolic analysis indicated that the contents of 108 metabolites significantly changed upon melatonin treatment, with the contents of those that are directly/indirectly involved in melatonin metabolism changing the most. Further, KEGG pathway analysis suggested that the metabolic pathways of amino acids, reducing sugar, secondary metabolites, indole alkaloid biosynthesis, purine, pyrimidine, and ABC transporters were greatly influenced by exogenous melatonin application. Moreover, metabolisms of melatonin-related antioxidants and pyrimidine nucleoside antibiotics were enhanced after melatonin treatment. Melatonin improved tobacco resistance to high salinity, drought, and extreme temperature stresses, as indicated by improved photosynthetic and antioxidant capacities in treated vs. untreated plants. This study lays a foundation for the comprehensive application of melatonin to increase the stress tolerance of plants.

PDGF-BB/KLF4/VEGF Signaling Axis in Pulmonary Artery Endothelial Cell Angiogenesis.

BACKGROUND: Accumulating evidence suggests that platelet-derived growth factor-BB (PDGF-BB) and vascular endothelial growth factor(VEGF) play a role in the progression of pulmonary arterial hypertension (PAH).Since chronic hypoxia is responsible for intimal hyperplasia and disordered angiogenesis of pulmonary arteries, which are histological hallmarks of PAH, we explored the role of the PDGF-BB/KLF4/VEGF signaling axis in the angiogenesis of pulmonary artery endothelial cells (PAECs). METHODS: Adult male Wistar rats were used to study hypoxia-induced or monocrotaline (MCT)-induced right ventricular (RV) remodeling as well as systolic function and hemodynamics using echocardiography and a pressure-volume admittance catheter. Morphometric analyses of lung vasculature and RV vessels were performed. RESULTS: The results revealed that both the PDGF receptor-tyrosine kinase inhibitor imatinib and the multi-targeted VEGF and PDGF receptor inhibit or sunitinib malate reversed hypoxia-induced increases in right ventricular systolic pressure (RVSP), right ventricular function and thickening of the medial walls. Mechanistically VEGF/VEGFR and PDGF/PDGFR formed a biological complex. We also showed that PDGF-BBincreasedKLF4 promoter activity transcriptionally activating VEGF expression, which regulates PAEC proliferation; migration; and the cell-cycle transition from G0/G1phase to S phase and G2/M-phase and eventually leads to PAEC angiogenesis Conclusion: Our study indicates that hypoxia-induced angiogenesis of PAECs is associated with increased levels of PDGF-BB/KLF4/VEGF, which contribute to pulmonary vascular remodeling. Overall, our study contributes to a better understanding of PAH pathogenesis.

Total saponin from Anemone flaccida Fr. Schmidt abrogates osteoclast differentiation and bone resorption via the inhibition of RANKL-induced NF-κB, JNK and p38 MAPKs activation.

Osteoclasts, bone-specialized multinucleated cells, are responsible for bone destructive diseases such as rheumatoid arthritis and osteoporosis. Natural plant-derived products have received substantial attention given their potential therapeutic and preventive activities against bone destructive diseases. In the present study, we investigated the effects of total saponin (TS) from Anemone flaccida Fr. Schmidt, on receptor activator of nuclear factor-κB ligand (RANKL)-induced in vitro osteoclast differentiation. We observed that TS concentration-dependently inhibited RANKL-induced osteoclast formation from RAW 264.7 cell and bone marrow-derived macrophages (BMMs), as well as decreased extent of actin ring formation and lacunar resorption. The RANKL-stimulated expression of osteoclast-related transcription factors were also diminished by TS. Moreover, TS blocked the RANKL-triggered TRAF6 expression, phosphorylation of mitogen-activated protein kinases (MAPKs) and IκB-α, and inhibited NF-κB p65 DNA binding activity. Furthermore, TS almost abrogated the nuclear factor of activated T cells (NFATc1) and c-Fos expression. Taken together, our results demonstrated that TS suppresses RANKL-induced osteoclast differentiation and inflammatory bone loss via the down-regulation of TRAF6 level, suppression of JNK and p38 MAPKs and NF-κB activation, and subsequent decreased expression of c-Fos and NFATc1. Therefore, TS may be a potential agent and needs to be more evaluated in vivo or in clinical trials to become a therapeutic for lytic bone diseases.

College students with depression symptom are more sensitive to task difficulty in reinforcement learning.

BACKGROUND: Depression is usually characterized by impairments in reward function, and shows altered motivation to reward in reinforcement learning. This study further explored whether task difficulty affects reinforcement learning in college students with and without depression symptom. METHODS: The depression symptom group (20) and the no depression symptom group (26) completed a probabilistic reward learning task with low, medium, and high difficulty levels, in which task the response bias to reward and the discriminability of reward were analyzed. Additionally, electrophysiological responses to reward and loss feedback were recorded and analyzed while they performed a simple gambling task. RESULTS: The depression symptom group showed more response bias to reward than the no depression symptom group when the task was easy and then exhibited more quickly decrease in response bias to reward as task difficulty increased. The no depression symptom group showed a decrease in response bias only in the high-difficulty condition. Further regression analyses showed that, the Feedback-related negativity (FRN) and theta oscillation could predict response bias change in the low-difficulty condition, the FRN and oscillations of theta and delta could predict response bias change in the medium and high-difficulty conditions. LIMITATIONS: The electrophysiological responses to loss and reward were not recorded in the same task as the reinforcement learning behaviors. CONCLUSIONS: College students with depression symptom are more sensitive to task difficulty during reinforcement learning. The FRN, and oscillations of theta and delta could predict reward leaning behavior.

Heading perception from optic flow occurs at both perceptual representation and working memory stages with EEG evidence.

Psychophysical studies have demonstrated that heading perception from optic flow occurs in perceptual and post-perceptual stages. The post-perception stage is a complex concept, containing working memory. The current study examined whether working memory was involved in heading perception from optic flow by asking participants to conduct a heading perception task and recording their scalp EEG. On each trial, an optic flow display was presented, followed by a blank display. Participants were then asked to report their perceived heading. We know that participants would tend to automatically forget previous headings when they learned that previously presented headings were unrelated to the current heading perception to save cognitive resources. As a result, we could not decode previous headings from the EEG data of current trials. More importantly, if we successfully decoded previous headings when the blank display (optic flow) was presented, then working memory (perceptual representation stage) was involved in heading perception. Our results showed that the decoding accuracy was significantly higher than the chance level when the optic flow and blank displays were presented. Therefore, the current study provided electrophysiological evidence that heading perception from optic flow occurred in the perceptual representation and working memory stages, against the previous perceptual claim.

The influence of the diffusion of responsibility effect on outcome evaluations: electrophysiological evidence from an ERP study.

Previous studies have revealed that personal responsibility has an influence on outcome evaluation, although the way this influence works is still unclear. This study imitated the phenomenon of responsibility diffusion in a laboratory to examine the influence of the effect of responsibility diffusion on the processing of outcome evaluation using the event-related potential (ERP) technique. Participants of the study were required to perform the gambling task individually in the high-responsibility condition and with others in the low-responsibility scenario. Self-rating results showed that the participants felt more responsible for monetary loss and believed that they had more contributions to the monetary gains in the high-responsibility condition than in the low-responsibility situation. Both the feedback-related negativity (FRN) and the P300 were sensitive to the responsibility level, as evidenced by the enhanced amplitudes in the high-responsibility condition for both components. Further correlation analysis showed a negative correlation between FRN amplitudes and subjective rating scores (i.e., the higher the responsibility level, the larger the FRN amplitude). The results probably indicate that the FRN and P300 reflect personal responsibility processing under the social context of diffusion of responsibility.

The influence of context condition on outcome evaluation in experimental conditions: Even vs. neutral.

Feedback-related negativity (FRN) is an event-related brain potential that is elicited during outcome evaluation. Studies have found that FRN reflects a good vs. bad classification; more importantly, FRN reflects this classification in a context-dependent manner, which means that the outcome evaluation is obviously influenced by its embedded context. In the current study, we examined how the context conditions of even (i.e., the feedback was +4 or -4) and neutral (i.e., the feedback was always 0) affect the outcome evaluation in experimental conditions (gain and loss). The experimental conditions of gain (i.e., the feedback was +4 or 0) and loss (i.e., the feedback was 0 or -4) were presented with the even condition as the context in one section and with the neutral condition as the context in another section. The ERP (event-related potential) results of the two experimental conditions showed that the unfavorable outcome evoked more negative FRN than the favorable outcome in both even and neutral sections, however, the amplitude difference between unfavorable and favorable outcomes was greater in neutral section than in even section. Furthermore, the favorable outcomes evoked more positive FRN in the neutral section than in the even section. These results indicate that the context condition modulates outcome evaluation, in neutral context, the discrimination between favorable and unfavorable outcome is better, which might due to the facilitated identification of favorable outcomes in neutral context.

Categorical perception of facial expressions in individuals with non-clinical social anxiety.

BACKGROUND AND OBJECTIVES: According to the well-established categorical perception (CP) of facial expressions, we decode complicated expression signals into simplified categories to facilitate expression processing. Expression processing deficits have been widely described in social anxiety (SA), but it remains to be investigated whether CP of expressions are affected by SA. The present study examined whether individuals with SA had an interpretation bias when processing ambiguous expressions and whether the sensitivity of their CP was affected by their SA. METHODS: Sixty-four participants (high SA, 30; low SA, 34) were selected from 658 undergraduates using the Interaction Anxiousness Scale (IAS). With the CP paradigm, specifically with the analysis method of the logistic function model, we derived the categorical boundaries (reflecting interpretation bias) and slopes (reflecting sensitivity of CP) of both high- and low-SA groups while recognizing angry-fearful, happy-angry, and happy-fearful expression continua. RESULTS: Based on a comparison of the categorical boundaries and slopes between the high- and low-SA groups, the results showed that the categorical boundaries between the two groups were not different for any of the three continua, which means that the SA does not affect the interpretation bias for any of the three continua. The slopes for the high-SA group were flatter than those for the low-SA group for both the angry-fearful and happy-angry continua, indicating that the high-SA group is insensitive to the subtle changes that occur from angry to fearful faces and from happy to angry faces. LIMITATIONS: Since participants were selected from a sample of undergraduates based on their IAS scores, the results cannot be directly generalized to individuals with clinical SA disorder. CONCLUSIONS: The study indicates that SA does not affect interpretation biases in the processing of anger, fear, and happiness, but does modulate the sensitivity of individuals' CP when anger appears. High-SA individuals perceive angry expressions in a less categorical manner than the low-SA group, but no such difference was found in the perception of happy or fearful expressions.

Give Me a Chance! Sense of Opportunity Inequality Affects Brain Responses to Outcome Evaluation in a Social Competitive Context: An Event-Related Potential Study.

People are strongly motivated to pursue social equality during social interactions. Previous studies have shown that outcome equality influences the neural activities of monetary feedback processing in socioeconomic games; however, it remains unclear whether perception of opportunity equality affects outcome evaluation even when outcomes are maintained equal. The current study investigated the electrophysiological activities of outcome evaluation in different instructed opportunity equality conditions with event-related potentials (ERPs). Participants were asked to play a competitive dice game against an opponent to win money. Opportunity equality was manipulated in three conditions, depending on whether participants were allowed the opportunity to throw less, equal, or more dice compared to their opponents. Although participants received a winning outcome with approximately 50% chance in all equality conditions, they selectively exhibited sensitivity to the less-dice condition by reporting stronger feelings of unfairness and unpleasantness than in the equal and more-dice conditions. In line with the behavioral results, larger reward positivity amplitudes were elicited by the monetary outcome in the less-dice condition than in the other two conditions, reflecting intensified reward prediction error (RPE) signals under negative emotional arousal. Further, P3 amplitudes were enhanced following reward feedback only in the unequal conditions, perhaps due to the high-level motivational and affective processing associated with resolving conflict between social norms and self-interest. The present findings elucidate the complex temporal course of outcome evaluation processes in different opportunity equality conditions.

Are we sensitive to valence differences in emotionally negative stimuli? Electrophysiological evidence from an ERP study.

The present study investigated whether the human brain is sensitive to valence differences in emotionally negative stimuli by recording event-related potentials (ERPs) for extremely negative (EN), moderately negative (MN), and neutral pictures while subjects perform a standard/deviant categorization task, irrespective of the emotional valence of the deviants. Using the same design, we also investigated the sensitivity of the human brain to valence differences in emotionally positive stimuli. Experiment 1 showed that EN stimuli elicited more negative deflections than MN stimuli in the early P2 and N2, later P3, and slow negative wave (SNW) components. In contrast, there were no differences in amplitude or latency in these components during the extremely positive (EP) and moderately positive (MP) conditions of Experiment 2. This suggests that humans are only sensitive to valence differences in negative stimuli, and that these negative valences could be processed differentially throughout the information processing stream even when individuals are highly engaged in a non-emotional task.

Detecting perceptual conflict by the feedback-related negativity in brain potentials.

The feedback-related negativity (FRN) in brain potentials is typically observed for the outcome evaluation concerning one's performance or monetary reward. Using a task in which the participant guesses whether the first stimulus (S1) would have the same color as the subsequently presented second stimulus (S2), this study demonstrates that the FRN to S2, which serves as feedback to the guessing, is also sensitive to the conflict between perceptual representations of S1 and S2 in working memory. The FRN effect for the feedback concerning the correctness of one's performance is modulated by the congruency between perceptual properties of the stimuli. The anterior cingulate cortex, which generates the FRN, is thus a general conflict-monitoring device detecting both response and perceptual conflicts.

Attentional resources modulate error processing-related brain electrical activity: Evidence from a dual-task design.

Attention plays an important role in the processing of error, but only a few studies have explored the relationship between them. The current study used a dual-task paradigm, combining the classic flanker task with a working memory load task, to explore how changes in the amount of attentional resources modulate error negativity (Ne) and error positivity (Pe). The results showed that the reduction of attentional resources overall caused a decrease in Pe amplitude, especially in the late stage of Pe, which had a significant diminution in amplitude. However, changes in the amount of attentional resources did not cause significant changes in the Ne amplitude. These results suggest that the early stage of error processing in the Ne time window is less affected by attention, but the Pe stage is regulated by attentional resources, especially in the late Pe stage.

Total Saponin from Anemone flaccida Fr. Schmidt Prevents Bone Destruction in Experimental Rheumatoid Arthritis via Inhibiting Osteoclastogenesis.

Anemone flaccida Fr. Schmidt is used in the clinical compound prescription for the treatment of rheumatoid arthritis (RA) in China and has the traditional use of draining dampness, diminishing swelling, and relieving pain. Total saponins (TS) are the characteristic components and also the main active ingredients of A. flaccida. Previous reports indicated that TS possess anti-inflammatory and immunoregulatory properties; however, the effects of TS on bone destruction of RA have not been evaluated. In this study, our data first showed the therapeutic effects of TS on severity of arthritis and arthritis progression in collagen-induced arthritis (CIA) rats. Then, by microfocal computed tomography (CT) quantification, TS significantly increased bone mineral density, bone volume fraction, and trabecular thickness and decreased trabecular separation of inflamed joints both at peri-articular and extra-articular locations. TS also diminished the level of the bone resorption marker CTX-I and simultaneously increased the bone formation marker osteocalcin in sera of CIA rats. Interestingly, TS prevented bone destruction by reducing the number of osteoclasts in inflamed joints, reducing the expression of receptor activator of nuclear factor-κF (RANK) ligand (RANKL) and RANK, increasing the expression of osteoprotegerin (OPG), at both mRNA and protein levels, and decreasing the ratio of RANKL to OPG in inflamed joints and sera of CIA rats. This was further confirmed in the co-culture system of human fibroblast-like synovial and peripheral blood mononuclear cells. In addition, TS inhibited the levels of pro-inflammatory cytokines implicated in bone resorption, such as interleukin-1ß (IL-1ß), tumor necrosis factor-α (TNFα), IL-6, IL-17, and IL-23 in sera and joints. These findings offer convincing evidence that TS attenuate RA partially by preventing both focal bone destruction and systemic bone loss. This anti-erosive effect results in part from inhibiting osteoclastogenesis by regulating the RANKL/RANK/OPG signaling pathway. The suppression of systemic and local pro-osteoclastogenic cytokines by TS was also highly effective.

Triterpenoid Saponin W3 from Anemone flaccida Suppresses Osteoclast Differentiation through Inhibiting Activation of MAPKs and NF-κB Pathways.

Excessive bone resorption by osteoclasts within inflamed joints is the most specific hallmark of rheumatoid arthritis. A. flaccida has long been used for the treatment of arthritis in folk medicine of China; however, the active ingredients responsible for the anti-arthritis effects of A. flaccida are still elusive. In this study, W3, a saponin isolated from the extract of A. flaccida was identified as the major active ingredient by using an osteoclast formation model induced by receptor activator of nuclear factor kappa-B ligand (RANKL). W3 dose-dependently suppressed the actin ring formation and lacunar resorption. Mechanistic investigation revealed that W3 inhibited the RANKL-induced TRAF6 expression, decreased phosphorylation of mitogen-activated protein kinases (MAPKs) and IκB-α, and suppressed NF-κB p65 DNA binding activity. Furthermore, W3 almost abrogated the expression of c-Fos and nuclear factor of activated T cells (NFATc1). Therefore, our results suggest that W3 is a potential agent for treating lytic bone diseases although further evaluation in vivo and in clinical trials is needed.

Right hemisphere advantage in processing Cantonese level and contour tones: evidence from dichotic listening.

The brain lateralization pattern of Cantonese tonal processing was examined with the dichotic listening (DL) paradigm. Three factors were manipulated systematically in the study. First, the processing of level tones was compared with that of contour tones. Second, the influence of a linguistic context in tonal processing was studied by contrasting the patterns of brain lateralization for real syllables, pseudo-syllables, and hums. Finally, the discrimination and the identification tasks were used to test how processing depth might modulate the results obtained. A right hemisphere advantage (RHA) was obtained regardless of tone type, stimulus type, and task. In addition, the performance on level tones was in general better than that on contour tones. These findings suggest that Cantonese speakers are highly sensitive to the acoustic features of lexical tones, which supports the acoustic view about tonal processing.

Attitude toward money modulates outcome processing: an ERP study.

Love of money (LOM) is concerned with the attitude toward money, which can be measured by the LOM scale through affective, behavioral, and cognitive dimensions. Research has observed that monetary attitude was tightly related to reward processing and could affect economic behavior. This study examined how monetary attitude modulated risky behavior and the underlying neural mechanisms of reward processing using event-related potential (ERP) technique. We compared both the risk level and brain responses of a high-level LOM (HLOM) group to a low-level LOM (LLOM) group using a simple gambling task. The behavioral results showed that the HLOM group was more risky than the LLOM group, particularly after loss. The feedback-related negativity (FRN) was measured as the difference wave (gain-related ERP was subtracted from loss-related ERP). The FRN difference wave was larger in the HLOM group than that in the LLOM group. The P3 in the HLOM group was more positive than that in the LLOM group. These results suggest that monetary attitude can modulate both the underlying neural mechanisms and behavioral performance in a reward-related task. The HLOM participants are more sensitive to gain/loss than the LLOM participants.

Cortical dynamics of semantic processing during sentence comprehension: evidence from event-related optical signals.

Using the event-related optical signal (EROS) technique, this study investigated the dynamics of semantic brain activation during sentence comprehension. Participants read sentences constituent-by-constituent and made a semantic judgment at the end of each sentence. The EROSs were recorded simultaneously with ERPs and time-locked to expected or unexpected sentence-final target words. The unexpected words evoked a larger N400 and a late positivity than the expected ones. Critically, the EROS results revealed activations first in the left posterior middle temporal gyrus (LpMTG) between 128 and 192 ms, then in the left anterior inferior frontal gyrus (LaIFG), the left middle frontal gyrus (LMFG), and the LpMTG in the N400 time window, and finally in the left posterior inferior frontal gyrus (LpIFG) between 832 and 864 ms. Also, expected words elicited greater activation than unexpected words in the left anterior temporal lobe (LATL) between 192 and 256 ms. These results suggest that the early lexical-semantic retrieval reflected by the LpMTG activation is followed by two different semantic integration processes: a relatively rapid and transient integration in the LATL and a relatively slow but enduring integration in the LaIFG/LMFG and the LpMTG. The late activation in the LpIFG, however, may reflect cognitive control.