RESUMO
BACKGROUND: Development of animal models has been one of the most remarkable achievements for Irritable Bowel Syndrome (IBS) research. These models need to exhibit face validity, construct and predictive validity, as well as internal (replicability) and external validity (generalizability). Among these models, chronic stress induced by water avoidance exposure (WA) has been validated in rats through increasing visceral hypersensitivity, motility impairment, anxiety and, colonic immune activity, all related to IBS. OBJECTIVE: To assess the external validity of WA indirectly on colonic motility in Wistar rats. METHOD: Ten male-Wistar rats were exposed to WA and compared with ten Wistar rats not exposed (controls). At the end of each exposure, the quantity of fecal pellets were determined and considered as a sign of autonomic regulation of colonic motility. Data was analyzed with a general linear model for repeated measures. RESULTS: Rats exposed to WA had higher number or pellets than controls: 7.46 ± 0.45 (95%CI: 6.51, 8.41) vs. 2.88 ± 0.45 (1.93, 3.83), p < 0.001. The higher number of pellets was related to WA exposure as there were no other significant interactions. In both groups, the number of pellets was higher during the first day and then decreased progressively. CONCLUSIONS: Chronic stress induced through WA in Wistar rats, exhibits external validity as an experimental model for IBS research and our findings of increased number of fecal pellets coincide with the appearance hypermotility related to IBS. The model is optimum for research studies on this disorder.
Assuntos
Modelos Animais de Doenças , Síndrome do Intestino Irritável , Ratos , Animais , Aprendizagem da Esquiva , Síndrome do Intestino Irritável/etiologia , Masculino , Ratos Wistar , Reprodutibilidade dos Testes , Estresse Psicológico , ÁguaRESUMO
INTRODUCTION: There have been studies in which contralesional inhibitory and ipsilesional excitatory transcranial magnetic stimulation (TMS) has been used as coadjuvant therapy in the motor rehabilitation of the thoracic limb in patients. However, there is no consensus regarding the stimulation variables or their clinical outcomes. AIM: To describe the results of clinical trials where TMS has been applied in rehabilitation in patients with cerebrovascular disease (CVD). PATIENTS AND METHODS: A systematic review of the PubMed database was performed. The articles cataloged as originals in English, whose population had limitation of thoracic limb after CVD were selected. Pilot studies, as well as studies in which patients under pharmacological treatment included any intervention other than physical or occupational therapy were excluded. Given their heterogeneity, it was not possible to apply inferential statistics, only descriptive statistics were use. RESULTS: Seven studies were identified; 259 cases with an age of 64.3 ± 4.28 years (range: 35-89 years) were reported. The TMS protocols, for the most part, were performed by contralesional inhibitory type stimulation. There were positive results in five studies. CONCLUSION: In accordance with the results obtained, we observed that TMS could contribute to the improvement of motor control of the thoracic limb in patients with sequelae due to CVD.
TITLE: Efecto de la estimulacion magnetica transcraneal sobre la recuperacion motora del miembro toracico en la enfermedad vascular cerebral. Revision sistematica.Introduccion. Existen estudios donde se ha utilizado la estimulacion magnetica transcraneal (EMT) inhibitoria contralesional y excitatoria ipsilesional como terapia coadyuvante en la rehabilitacion motora del miembro toracico; sin embargo, no existe un consenso respecto a las variables de estimulacion ni sobre sus resultados clinicos. Objetivo. Describir los resultados de los ensayos clinicos donde se ha aplicado EMT en la rehabilitacion en pacientes con enfermedad vascular cerebral (EVC). Pacientes y metodos. Se realizo la revision sistematica de la base de datos PubMed. Fueron seleccionados los estudios catalogados como originales en idioma ingles, cuya poblacion tuvo afectacion de miembro toracico tras una EVC. Se excluyeron todos los estudios piloto, ademas de estudios que incluyeran pacientes bajo tratamiento farmacologico o alguna intervencion diferente a terapia fisica u ocupacional. Dada la heterogeneidad percibida en los estudios, no fue posible aplicar estadistica inferencial, unicamente se empleo estadistica descriptiva. Resultados. Fueron seleccionados siete estudios. Se identificaron 259 casos con una edad media de 64,3 ± 4,28 años (rango: 35-89 años). Los protocolos de EMT, en su mayoria, se realizaron mediante estimulacion de tipo inhibitoria contralesional. Hubo resultados positivos en cinco estudios. Conclusion. De acuerdo con los resultados obtenidos, existen indicios de que la EMT podria contribuir a la mejoria del control motor del miembro toracico en los pacientes con secuelas por EVC.
Assuntos
Acidente Vascular Cerebral/terapia , Estimulação Magnética Transcraniana , Extremidade Superior/fisiologia , Humanos , Destreza Motora , Recuperação de Função Fisiológica , Reabilitação do Acidente Vascular Cerebral/métodosRESUMO
INTRODUCTION: Since, under certain circumstances, defensive or attacking behaviours display a pattern of motor dominance, as observed in subjects who participate in contact or fighting sports, aggressive behaviour was considered to have a dominant motor pattern. With the aim of preventing the functional problems reported with bilateral lesion procedures involving both the central nucleus of the amygdala and the posteromedial hypothalamus, the decision was made to combine them; thus, an amygdalotomy of the central nucleus of the amygdala and a posteromedial hypothalamotomy were to be performed simultaneously and unilaterally, on the basis of the motor dominance of the patient determined by means of the Edinburgh test. PATIENTS AND METHODS: This study describes the surgical experience in a series of nine patients diagnosed with refractory neuroaggressive syndrome. As part of the study protocol, a magnetic resonance brain scan was performed to rule out the presence of neoplasms, vascular diseases, infections and degenerative disorders. The degree of aggressiveness was quantified using Yudofsky's Overt Aggression Scale. Additionally, manual dominance was determined by means of the Edinburgh test. RESULTS AND CONCLUSIONS: Good control of aggressiveness was seen immediately. In some cases it was necessary to reduce the antipsychotic or benzodiazepine medication, as it was seen to increase aggressiveness. Only one case required a second surgical intervention. Follow-up was achieved in 100% of the cases at 24 months and 78% at 36 months.
TITLE: Tratamiento de la agresividad refractaria mediante amigdalotomia e hipotalamotomia posteromedial por radiofrecuencia.Introduccion. Dado que, en algunas circunstancias, las conductas defensivas o de ataque muestran un patron de dominancia motora, tal como se observa en los sujetos dedicados a los deportes de contacto o de lucha, se considero que la conducta agresiva tiene un patron motor dominante. Con el fin de evitar los problemas funcionales descritos con los procedimientos de lesion bilateral tanto del nucleo central de la amigdala como del hipotalamo posteromedial, se decidio combinarlos; es decir, realizar amigdalotomia del nucleo central de la amigdala e hipotalamotomia posteromedial de manera unilateral y simultanea, basandose en la dominancia motora del paciente mediante la prueba de Edimburgo. Pacientes y metodos. Este estudio muestra la experiencia quirurgica en una serie de nueve pacientes con el diagnostico de sindrome neuroagresivo resistente al tratamiento farmacologico. Dentro del protocolo de estudio, se les realizo resonancia magnetica cerebral para descartar la presencia de neoplasias, enfermedades vasculares, infecciones y trastornos degenerativos. El grado de agresividad se cuantifico mediante la escala global de agresividad de Yudofsky. Adicionalmente, se determino la dominancia manual a traves de la prueba de Edimburgo. Resultados y conclusiones. El buen control de la agresividad se observo de modo inmediato. En algunos casos fue necesario reducir la medicacion de antipsicoticos o benzodiacepinas, ya que aumentaban la agresividad. Solo un caso requirio una segunda cirugia. Se logro seguimiento del 100% de los casos en 24 meses y del 78% en 36 meses.