RESUMO
Greig cephalopolysyndactyly syndrome (GCPS) is an autosomal dominant disorder affecting limb and craniofacial development. Recently, the human GLI3 gene has been proposed to be a candidate gene for GCPS. Here we describe the molecular characterization of extra-toes (Xt), which is a mouse model of GCPS. The Xt heterozygotes show craniofacial defects and a polydactyly phenotype similar to GCPS. We show that a deficiency of Gli3 expression in the XtJ mutant is due to a deletion within the 3' end of the gene. Furthermore, structures affected in the mouse mutant and human syndrome were found to correlate with expression domains of Gli3 in mouse. These results strongly suggest that the deficiency of GLI3 function leads to GCPS.
Assuntos
Ossos Faciais/anormalidades , Deleção de Genes , Íntrons , Crânio/anormalidades , Sindactilia/genética , Alelos , Animais , Southern Blotting , DNA/genética , DNA/isolamento & purificação , Modelos Animais de Doenças , Embrião de Mamíferos/patologia , Ossos Faciais/embriologia , Homozigoto , Humanos , Camundongos , Camundongos Endogâmicos C3H , Camundongos Mutantes , Fenótipo , Crânio/embriologia , Sindactilia/embriologia , SíndromeRESUMO
Huntington's disease (HD) is an autosomal-dominant neurodegenerative disorder caused by a CAG repeat expansion that lengthens a glutamine segment in the novel huntingtin protein. To elucidate the molecular basis of HD, we extended the polyglutamine tract of the mouse homologue, Hdh, by targetted introduction of an expanded human HD CAG repeat, creating mutant HdhneoQ50 and HdhQ50 alleles that express reduced and wild-type levels of altered huntingtin, respectively. Mice homozygous for reduced levels displayed characteristic aberrant brain development and perinatal lethality, indicating a critical function for Hdh in neurogenesis. However, mice with normal levels of mutant huntingtin did not display these abnormalities, indicating that the expanded CAG repeat does not eliminate or detectably impair huntingtin's neurogenic function. Thus, the HD defect in man does not mimic complete or partial Hdh inactivation and appears to cause neurodegenerative disease by a gain-of-function mechanism.
Assuntos
Doença de Huntington/genética , Doença de Huntington/patologia , Proteínas do Tecido Nervoso/genética , Proteínas Nucleares/genética , Sequências Repetitivas de Ácido Nucleico/genética , Alelos , Animais , Diferenciação Celular/genética , Desenvolvimento Embrionário e Fetal/genética , Deleção de Genes , Heterozigoto , Homozigoto , Humanos , Proteína Huntingtina , Masculino , Camundongos , Camundongos Mutantes , Camundongos Transgênicos , Mutagênese Insercional , Proteínas do Tecido Nervoso/metabolismo , Proteínas Nucleares/metabolismo , FenótipoRESUMO
The mouse gene Mash2 encodes a transcription factor required for development of trophoblast progenitors. Mash2-homozygous mutant embryos die at 10 days postcoitum from placental failure. Here we show that Mash2 is genomically imprinted. First, Mash2+/- embryos inheriting a wild-type allele from their father die at the same stage as -/- embryos, with a similar placental phenotype. Second, the Mash2 paternal allele is initially expressed by groups of trophoblast cells at 6.5 and 7.5 days post-coitum, but appears almost completely repressed by 8.5 days post-coitum. Finally, we have genetically and physically mapped Mash2 to the distal region of chromosome 7, within a cluster of imprinted genes, including insulin-2, insulin-like growth factor-2 and H19.
Assuntos
Proteínas de Ligação a DNA/genética , Impressão Genômica , Fatores de Transcrição , Trofoblastos/metabolismo , Alelos , Animais , Sequência de Bases , Fatores de Transcrição Hélice-Alça-Hélice Básicos , Mapeamento Cromossômico , Cruzamentos Genéticos , Primers do DNA/genética , Feminino , Regulação da Expressão Gênica no Desenvolvimento , Ligação Genética , Idade Gestacional , Heterozigoto , Homozigoto , Masculino , Camundongos , Dados de Sequência Molecular , Mutação , GravidezRESUMO
Fanconi anaemia (FA) is an autosomal recessive disease characterized by bone marrow failure, variable congenital malformations and predisposition to malignancies. Cells derived from FA patients show elevated levels of chromosomal breakage and an increased sensitivity to bifunctional alkylating agents such as mitomycin C (MMC) and diepoxybutane (DEB). Five complementation groups have been identified by somatic cell methods, and we have cloned the gene defective in group C (FAC)(7). To understand the in vivo role of this gene, we have disrupted murine Fac and generated mice homozygous for the targeted allele. The -/- mice did not exhibit developmental abnormalities nor haematologic defects up to 9 months of age. However, their spleen cells had dramatically increased numbers of chromosomal aberrations in response to MMC and DEB. Homozygous male and female mice also had compromised gametogenesis, leading to markedly impaired fertility, a characteristic of FA patients. Thus, inactivation of Fac replicates some of the features of the human disease.
Assuntos
Anemia de Fanconi/genética , Infertilidade/genética , Mutação , Animais , Clonagem Molecular , Feminino , Marcação de Genes , Genes Recessivos , Vetores Genéticos , Homozigoto , Infertilidade/patologia , Masculino , Camundongos , Ovário/patologia , Testículo/patologiaRESUMO
A decade ago, chick-quail transplantation studies demonstrated that the junction between the midbrain and hindbrain has the properties of an organizing center capable of patterning the midbrain and cerebellum. Many of the genes that function to pattern these tissues have been identified and extensively studied. Recent experiments have shown that Otx2, Gbx2 and Fgf8 genes play a major role in the positioning and functioning of this organizing center.
Assuntos
Encéfalo/metabolismo , Fatores de Crescimento de Fibroblastos/metabolismo , Proteínas de Homeodomínio/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Organizadores Embrionários/metabolismo , Transativadores/metabolismo , Proteínas de Peixe-Zebra , Animais , Padronização Corporal/genética , Encéfalo/embriologia , Cerebelo/embriologia , Cerebelo/metabolismo , Indução Embrionária/genética , Retroalimentação/fisiologia , Fator 8 de Crescimento de Fibroblasto , Fatores de Crescimento de Fibroblastos/genética , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Homeodomínio/genética , Mesencéfalo/embriologia , Mesencéfalo/metabolismo , Camundongos , Proteínas do Tecido Nervoso/genética , Fatores de Transcrição Otx , Prosencéfalo/embriologia , Prosencéfalo/metabolismo , Proteínas Proto-Oncogênicas/metabolismo , Rombencéfalo/embriologia , Rombencéfalo/metabolismo , Transativadores/genética , Proteínas WntRESUMO
Glucagon-like peptide 1 (GLP1) is postulated to regulate blood glucose and satiety, but the biological importance of GLP1 as an incretin and neuropeptide remains controversal. The regulation of nutrient-induced insulin secretion is dependent on the secretion of incretins, gut-derived peptides that potentiate insulin secretion from the pancreatic islets. To ascertain the relative physiological importance of GLP1 as a regulator of feeding behavior and insulin secretion, we have generated mice with a targeted disruption of the GLP1 receptor gene (GLP1R). These GLP1R-/- mice are viable, develop normally but exhibit increased levels of blood glucose following oral glucose challenge in association with diminished levels of circulating insulin. It is surprising that they also exhibit abnormal levels of blood glucose following intraperitoneal glucose challenge. Intracerebroventricular administration of GLP1 inhibited feeding in wild-type mice but not in GLP1R-/- mice; however, no evidence for abnormal body weight or feeding behavior was observed in GLP1R-/- mice. These observations demonstrate that GLP1 plays a central role in the regulation of glycemia; however, disruption of GLP1/GLP1R signaling in the central nervous system is not associated with perturbation of feeding behavior or obesity in vivo.
Assuntos
Glicemia/análise , Receptores de Glucagon/metabolismo , Animais , Feminino , Deleção de Genes , Glucagon/farmacologia , Peptídeo 1 Semelhante ao Glucagon , Receptor do Peptídeo Semelhante ao Glucagon 1 , Glucose/metabolismo , Glucose/farmacologia , Intolerância à Glucose , Insulina/sangue , Masculino , Camundongos , Fragmentos de Peptídeos/farmacologia , Precursores de Proteínas/farmacologia , Ratos , Receptores de Glucagon/genéticaRESUMO
A strategy was devised for identifying regions of the mouse genome that are transcriptionally active in a temporally and spatially restricted manner during development. The approach is based on the introduction into embryonic stem cells of two types of lacZ reporter constructs that can be activated by flanking mouse genomic sequences. Embryonic stem cells containing the lacZ constructs were used to produce chimaeric mice. Developmental regulation of lacZ expression occurred at a high frequency. Molecular cloning of the flanking endogenous genes and introduction of these potential insertional mutations into the mouse germ line should provide an efficient means of identifying and mutating novel genes important for the control of mammalian development.
Assuntos
Embrião de Mamíferos/metabolismo , Galactosidases/genética , Regulação da Expressão Gênica , Células-Tronco/metabolismo , beta-Galactosidase/genética , Animais , Linhagem Celular , Quimera , Clonagem Molecular , Vetores Genéticos , Células Germinativas , Proteínas de Choque Térmico/genética , Masculino , Camundongos , Regiões Promotoras Genéticas , Transfecção , Transformação GenéticaRESUMO
The two mouse genes, En-1 and En-2, that are homologs of the Drosophila segmentation gene engrailed, show overlapping spatially restricted patterns of expression in the neural tube during embryogenesis, suggestive of a role in regional specification. Mice homozygous for a targeted mutation that deletes the homeobox were viable and showed no obvious defects in embryonic development. This may be due to functional redundancy of En-2 and the related En-1 gene product during embryogenesis. Consistent with this hypothesis, the mutant mice showed abnormal foliation in the adult cerebellum, where En-2, and not En-1, is normally expressed.
Assuntos
Cerebelo/anatomia & histologia , Deleção Cromossômica , Genes Homeobox , Animais , Blastocisto , Linhagem Celular , Cerebelo/embriologia , Cerebelo/patologia , Quimera , Feminino , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Sistema Nervoso/embriologia , FenótipoRESUMO
The related mouse Engrailed genes En-1 and En-2 are expressed from the one- and approximately five-somite stages, respectively, in a similar presumptive mid-hindbrain domain. However, mutations in En-1 and En-2 produce different phenotypes. En-1 mutant mice die at birth with a large mid-hindbrain deletion, whereas En-2 mutants are viable, with cerebellar defects. To determine whether these contrasting phenotypes reflect differences in temporal expression or biochemical activity of the En proteins, En-1 coding sequences were replaced with En-2 sequences by gene targeting. This rescued all En-1 mutant defects, demonstrating that the difference between En-1 and En-2 stems from their divergent expression patterns.
Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Marcação de Genes , Genes Homeobox , Proteínas de Homeodomínio/genética , Proteínas do Tecido Nervoso/genética , Animais , Encéfalo/anormalidades , Encéfalo/embriologia , Quimera , Cruzamentos Genéticos , Feminino , Proteínas de Homeodomínio/fisiologia , Deformidades Congênitas dos Membros , Masculino , Camundongos , Mutação , Proteínas do Tecido Nervoso/fisiologia , Fenótipo , Regiões Promotoras Genéticas , Recombinação Genética , Células-Tronco , Esterno/anormalidadesRESUMO
Huntington's disease (HD) is a dominant neurodegenerative disorder caused by expansion of a CAG repeat in the gene encoding huntingtin, a protein of unknown function. To distinguish between "loss of function" and "gain of function" models of HD, the murine HD homolog Hdh was inactivated by gene targeting. Mice heterozygous for Hdh inactivation were phenotypically normal, whereas homozygosity resulted in embryonic death. Homozygotes displayed abnormal gastrulation at embryonic day 7.5 and were resorbing by day 8.5. Thus, huntingtin is critical early in embryonic development, before the emergence of the nervous system. That Hdh inactivation does not mimic adult HD neuropathology suggests that the human disease involves a gain of function.
Assuntos
Doença de Huntington/genética , Proteínas do Tecido Nervoso/genética , Proteínas Nucleares/genética , Animais , Sequência de Bases , Linhagem Celular , Ectoderma/citologia , Desenvolvimento Embrionário e Fetal , Feminino , Marcação de Genes , Genótipo , Heterozigoto , Homozigoto , Humanos , Proteína Huntingtina , Masculino , Mesoderma/citologia , Camundongos , Camundongos Endogâmicos C57BL , Dados de Sequência Molecular , Proteínas do Tecido Nervoso/fisiologia , Proteínas Nucleares/fisiologia , Fenótipo , Células-Tronco/metabolismoRESUMO
A curious feature of organ and organoid morphogenesis is that in certain cases, spatial oscillations in the thickness of the growing "film" are out of phase with the deformation of the slower-growing "substrate," while in other cases, the oscillations are in phase. The former cannot be explained by elastic bilayer instability, and contradict the notion that there is a universal mechanism by which brains, intestines, teeth, and other organs develop surface wrinkles and folds. Inspired by the microstructure of the embryonic cerebellum, we develop a new model of 2D morphogenesis in which system-spanning elastic fibers endow the organ with a preferred radius, while a separate fiber network resides in the otherwise fluidlike film at the outer edge of the organ and resists thickness gradients thereof. The tendency of the film to uniformly thicken or thin is described via a "growth potential." Several features of cerebellum, +blebbistatin organoid, and retinal fovea morphogenesis, including out-of-phase behavior and a film thickness amplitude that is comparable to the radius amplitude, are readily explained by our simple analytical model, as may be an observed scale invariance in the number of folds in the cerebellum. We also study a nonlinear variant of the model, propose further biological and bioinspired applications, and address how our model is and is not unique to the developing nervous system.
RESUMO
The mouse midbrain-hindbrain constriction is centrally involved in patterning of the midbrain and anterior hindbrain (cerebellum), as revealed by recent genetic studies using mice and embryological studies in chick (reviewed in [1,2]). This region can act as an organizer region to induce midbrain and cerebellar development. Genes such as Engrailed-1, Pax-2 and Pax-5, which are expressed in the embryonic cells that will form the midbrain and the cerebellum, are required for development of these regions. Fate-mapping experiments at early somite stages in chick have revealed that the cerebellar primordium is located both anterior and posterior to the midbrain-hindbrain constriction, whereas midbrain precursors lie more anteriorly. Fate mapping in mice has been complicated by the inaccessibility of the postimplantation embryo. Here, we report the use of a new in vivo approach involving the Cre-IoxP site-specific recombination system [3] to map the fate of cells in the mouse midbrain-hindbrain constriction. We show that cells originating in the mouse dorsal midbrain-hindbrain constriction during embryonic days 9-12 contribute significantly to the medial cerebellum and colliculi. Our data demonstrate the feasibility of using a recombinase-based lineage-tracing system for fate mapping in the mouse.
Assuntos
Desenvolvimento Embrionário e Fetal/genética , Mesencéfalo/embriologia , Recombinação Genética , Rombencéfalo/embriologia , Proteínas Virais , Animais , Embrião de Galinha , Cruzamentos Genéticos , Feminino , Regulação da Expressão Gênica no Desenvolvimento , Genes Reporter , Proteínas de Homeodomínio/genética , Integrases/genética , Óperon Lac , Masculino , Camundongos , Camundongos Transgênicos , Proteínas do Tecido Nervoso/genética , GravidezRESUMO
The mouse Engrailed, Wnt and Pax genes, which are homologues of Drosophila segmentation genes, have provided a critical genetic entry point for dissecting the molecular and cellular control of mesencephalon and metencephalon development in vertebrates. Mutant phenotypes and gene expression data suggest that six members of these gene families are required for early formation of these brain regions. Ectopic transplantation studies have shown that the midbrain-hindbrain-junction protein can act as an organizer and recruit certain host cells to re-establish parts of the entire region. Taken together, these studies indicate that the mesencephalon and metencephalon develop as one independent unit, and that the genetic network regulating development of this region involves conserved genes that control segmentation in Drosophila. By analogy, segmentation of the rest of the brain might best be described in terms of 'genetic units' defined by genetic and transplantation data.
Assuntos
Proteínas de Ligação a DNA/genética , Proteínas de Homeodomínio/genética , Mesencéfalo/embriologia , Proteínas do Tecido Nervoso/genética , Proteínas Nucleares/genética , Proteínas Proto-Oncogênicas/genética , Rombencéfalo/embriologia , Fatores de Transcrição , Proteínas de Peixe-Zebra , Animais , Proteínas de Homeodomínio/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Fator de Transcrição PAX5 , Proteínas WntRESUMO
The mouse has been a slower starter than many other organisms in the race to unravel the genetic control of embryonic development. Recent cloning of putative developmental genes combined with new approaches to manipulating the mouse genome seem set, however, to allow the mammalian embryo to move towards the front of the field.
Assuntos
Genes Reguladores , Mamíferos/crescimento & desenvolvimento , Animais , Clonagem Molecular , Desenvolvimento Embrionário e Fetal , Mamíferos/genética , Camundongos , Morfogênese , Mutação , Recombinação GenéticaRESUMO
We describe the generation of infectious retroviruses containing foreign genes by an in vivo recombination-deletion mechanism. Cotransfection into mouse cells of chimeric plasmids carrying a murine retrovirus 5' long terminal repeat and either the thymidine kinase (tk) gene of herpesvirus or the dominant selectable bacterial gene for neomycin resistance (neo), along with a clone of Moloney murine leukemia virus, results in the generation of infectious thymidine kinase or neomycin-resistant viruses. Expression of the selectable marker in these viruses can be regulated by the homologous transcriptional promoter of the gene, by the promoter contained within the Friend spleen focus-forming virus long terminal repeat, or by the simian virus 40 early region promoter. In all cases, the rescued viruses appeared to arise by recombination in vivo with Moloney murine leukemia virus sequences, resulting in the acquisition of the Moloney 3' long terminal repeat and variable amounts of the 3' adjacent Moloney genome. In two of the thymidine kinase constructs where tk was inserted 200 base pairs downstream from the long terminal repeat, the rescued viruses acquired a large part of the murine leukemia virus genome, including the region involved in packaging genomic RNA into virions. The generation of infectious neomycin-resistant virus is associated with deletions of simian virus 40 splicing and polyadenylation sequences. These results demonstrate that nonhomologous recombination and deletion events can take place in animal cells, resulting in the acquisition or removal of cis-acting sequences required for, or inhibitory to, retrovirus infectivity.
Assuntos
Transformação Celular Viral , Genes Dominantes , Genes Virais , Vírus Auxiliares/genética , Vírus da Leucemia Murina de Moloney/genética , Transdução Genética , Adenina Fosforribosiltransferase/genética , Animais , Sequência de Bases , Enzimas de Restrição do DNA , DNA Recombinante/metabolismo , Vetores Genéticos , Células L/enzimologia , Camundongos , Hibridização de Ácido Nucleico , Plasmídeos , Timidina Quinase/genéticaRESUMO
A series of deletions and insertions utilizing the herpesvirus thymidine kinase gene (tk) were constructed in the murine retrovirus Friend spleen focus-forming virus (SFFV). In all cases, the coding region for the SFFV-specific glycoprotein (gp55), which is implicated in erythroleukemic transformation, was left intact. These SFFV-TK and SFFV deletion vectors were analyzed for expression of tk and gp55 after DNA-mediated gene transfer. In addition, virus rescued by cotransfection of these vectors with Moloney murine leukemia virus was analyzed for infectious TK-transducing virus, gp55 expression, and erythroleukemia-inducing ability. The experiments demonstrated that deletions or insertions within the intron for the gp55 env gene can interfere with expression of gp55 after both DNA-mediated gene transfer and virus infection. In contrast, the gene transfer efficiency of the tk gene was unaffected in the SFFV-TK vectors, and high-titer infectious TK virus could be recovered. Revertant viruses capable of inducing erythroleukemia and expressing gp55 were generated after cotransfection of the SFFV-TK vectors with murine leukemia virus. The revertant viruses lost both tk sequences and the ability to transduce TK- fibroblasts to a TK+ phenotype. These experiments demonstrate that segregation of the TK and erythroleukemia functions can occur in retrovirus vectors which initially carry both markers.
Assuntos
Transformação Celular Viral , Vírus da Leucemia Murina de Friend/genética , Genes Virais , Genes , Vetores Genéticos , Simplexvirus/genética , Timidina Quinase/genética , Transdução Genética , Animais , Deleção Cromossômica , Células L/enzimologia , Camundongos , Fenótipo , Simplexvirus/enzimologia , TransfecçãoRESUMO
Gene targeting provides a means of directly assaying the function of specific genes during mouse nervous system development. Generation of targeted mutant mice has provided the first evidence of developmental roles for genes whose function was suggested based on their expression, but for which appropriate assay systems were lacking. In other cases, where gene function was known, targeted mutations have revealed in which cell population, and at what developmental stage, particular genes are first indispensable. The existing targeted mutants suggest that an early mechanism of pattern formation in mammals involves regional control of proliferation or survival of neural precursors, and that later general functions, such as the control of differentiation of precursors, may be performed by different genes in distinct neural lineages. As many genes display complex temporal and spatial patterns of expression, analysis of the full range of functions of such genes will require the generation of a series of alleles, including stage- and tissue-specific mutations.
Assuntos
Genes , Sistema Nervoso/embriologia , Animais , Diferenciação Celular , Divisão Celular , Sobrevivência Celular , Sistema Nervoso Central/embriologia , Humanos , Camundongos , Camundongos Mutantes Neurológicos , Neurônios/citologia , Células-Tronco/citologiaRESUMO
With the realization that many proto-oncogenes and tumor suppressor genes are expressed and have important functions during mammalian development, it is clear that cancer often involves the inappropriate activation of genetic pathways used during normal development. A signaling cascade that has been of considerable interest to both developmental and cancer biologists involves the Hedgehog (Hh) family of secreted proteins. To date, the only transcription factors shown to be directly downstream of Hh are the zinc-finger containing proteins Cubitus interruptus (Ci) and Gli, in flies and vertebrates, respectively. The identification of many of the genes and proteins involved in Hh signaling has come largely from genetic and biochemical studies in Drosophila. Ci mediates Hh signaling through a Hh-dependent set of protein modifications that alter the activity of Ci on Hh target genes. Recent evidence suggests vertebrate Gli proteins may be similarly regulated. The interest in this pathway has taken on added importance with the identification of mutations in Hh pathway genes, including Gli genes, in several human developmental disorders and cancers. We discuss models for how Gli proteins mediate Hh signaling in both vertebrate development and cancers.
Assuntos
Transformação Celular Neoplásica , Regulação da Expressão Gênica no Desenvolvimento , Genes Supressores de Tumor , Proteínas Oncogênicas/genética , Proto-Oncogenes , Transativadores , Fatores de Transcrição/genética , Animais , Drosophila , Proteínas Hedgehog , Humanos , Proteínas/genética , Proteínas/metabolismo , Vertebrados , Proteína GLI1 em Dedos de ZincoRESUMO
The Drosophila Achaete-Scute Complex genes encode transcriptional regulators belonging to the basic-helix-loop-helix family which control early steps of development of the central and peripheral nervous systems. We have isolated two mouse homologues of Achaete-Scute Complex genes, Mash-1 and Mash-2, by using the conservation of the basic-helix-loop-helix domain in this family. In this article, we analyse the expression of Mash-1 from its onset during neurulation to adult stages by RNA in situ hybridization on whole mounts and sections. As was observed for the rat Mash-1 protein, mouse Mash-1 RNA expression is restricted to cells of the developing central and peripheral nervous systems. We have observed three successive phases in the distribution of Mash-1 transcripts in the developing central nervous system. Initially, between embryonic day 8.5 and 10.5, Mash-1 transcripts are found in restricted domains in the neuroepithelium of the midbrain and ventral forebrain, as well as in the spinal cord. Between embryonic day 10.5 and 12.5, Mash-1 expression pattern changes from a restricted to a widespread one. Mash-1 transcripts are then found at variable levels in the ventricular zone in all regions of the brain. From embryonic day 12.5 to post-natal stages, Mash-1 is also expressed in cells outside of the ventricular zone throughout the brain. In addition, Mash-1 is expressed during development of the olfactory epithelium and neural retina. Overall, its expression pattern suggest that Mash-1 plays a role at early stages of development of specific neural lineages in most regions of the central nervous system and of several lineages in the peripheral nervous system. We have also compared the expression of Mash-1 and mouse Notch because their Drosophila homologues have been shown to interact genetically. The two genes show very similar expression patterns, both spatially and temporally, in the early developing brain and in the retina, suggesting that both genes may participate in the development of the same neural lineages.
Assuntos
Sistema Nervoso Central/embriologia , Drosophila/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Sistema Nervoso Central/crescimento & desenvolvimento , Clonagem Molecular , Desenvolvimento Embrionário e Fetal/genética , Epitélio/embriologia , Epitélio/crescimento & desenvolvimento , Expressão Gênica , Sequências Hélice-Alça-Hélice , Camundongos , Dados de Sequência Molecular , Bulbo Olfatório/embriologia , Bulbo Olfatório/crescimento & desenvolvimento , Estrutura Terciária de Proteína , Retina/crescimento & desenvolvimento , Retina/metabolismo , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido NucleicoRESUMO
During early brain development mouse Engrailed2 (En2) is expressed in a broad band across most of the mid-hindbrain region. Evidence from gene expression data, promoter analysis in transgenic mice and mutant phenotype analysis in mice and zebrafish has suggested that Pax2, 5 and 8 play a critical role in regulating En2 mid-hindbrain expression. Previously, we identified two Pax2/5/8-binding sites in a 1.0 kb En2 enhancer fragment that is sufficient to directed reporter gene expression to the early mid-hindbrain region and showed that the two Pax2/5/8-binding sites are essential for the mid-hindbrain expression in transgenic mice. In the present study we have examined the functional requirements of these two Pax2/5/8-binding sites in the context of the endogenous En2 gene for directing mid-hindbrain expression. The two Pax2/5/8-binding sites were deleted from the En2 locus and replaced with the bacterial neo gene by homologous recombination in mouse embryonic stem cells. After transmitting the mutation into mice, the neo gene was removed by breeding with transgenic mice expressing cre from a CMV promoter. Embryos homozygous for this En2 Pax2/5/8-binding site deletion mutation had a mild reduction in En2 expression in the presumptive mid-hindbrain region at the 5-7 somite stage, when En2 expression is normally initiated. However, from embryonic day 9.0 onwards, the mutant embryos showed En2 expression indistinguishable from that seen in wild type embryos. Furthermore, the mutants did not show the cerebellar defect seen in mice with a null mutation in En2. This result demonstrates that the two Pax2/5/8-binding sites that were deleted, while being required for mid-hindbrain expression in the context of a 1.0 kb En2 enhancer, are only required for proper initiation of expression of the endogenous En2 gene. Interestingly, a comparison of the lacZ RNA and protein expression patterns directed by the 1.0 kb enhancer fragment revealed that lacZ protein was acting as a lineage marker in the mid-hindbrain region by persisting longer than the mRNA. The transgene expression directed by the 1.0 kb enhancer fragment therefore does not mimic the entire broad domain of En2 expression. Taken together, these two studies demonstrate that DNA binding sites in addition to the two Pax2/5/8-binding sites must be necessary for En2 mid-hindbrain expression.