Capillary-Driven Rise of Well-Wetting Liquid on the Outer Surface of Cylindrical Nozzles.

Well-wetting liquids exiting small-diameter nozzles in the dripping regime can partially rise up along the outer nozzle surfaces. This is problematic for fuel injectors and other devices such as direct-contact heat and mass exchangers that incorporate arrays of nozzles to distribute liquids. We report our experimental and numerical study of the rising phenomenon for wide ranges of parameters. Our study shows that the interplay of three dimensionless numbers (the Bond number, the Weber number, and the Ohnesorge number) governs the capillary-driven rise dynamics. In general, as the flow rate or the viscosity increases, the capillary-driven rise height over each dripping period becomes smaller. We identify liquid flow rates below which the temporal evolution of the meniscus positions can be well approximated by a quasistatic model based on the Young-Laplace equation. Our analysis reveals two critical Bond numbers that give nozzle sizes, which correspond to the maximum meniscus rise and the onset of capillary-driven rise cessation. These critical Bond numbers are characterized as a function of the contact angle, regardless of the fluid type. Our study leads to a more efficient and optimized nozzle design in systems using wetting liquids by reducing both the risks of contamination and high pressure drop in such devices.

Susceptibility of Primary Sensory Cortex to Spreading Depolarizations.

UNLABELLED: Spreading depolarizations (SDs) are recognized as actors in neurological disorders as diverse as migraine and traumatic brain injury (TBI). Migraine aura involves sensory percepts, suggesting that sensory cortices might be intrinsically susceptible to SDs. We used optical imaging, MRI, and field potential and potassium electrode recordings in mice and electrocorticographic recordings in humans to determine the susceptibility of different brain regions to SDs. Optical imaging experiments in mice under isoflurane anesthesia showed that both cortical spreading depression and terminal anoxic depolarization arose preferentially in the whisker barrel region of parietal sensory cortex. MRI recordings under isoflurane, ketamine/xylazine, ketamine/isoflurane, and urethane anesthesia demonstrated that the depolarizations did not propagate from a subcortical source. Potassium concentrations showed larger increases in sensory cortex, suggesting a mechanism of susceptibility. Sensory stimulation biased the timing but not the location of depolarization onset. In humans with TBI, there was a trend toward increased incidence of SDs in parietal/temporal sensory cortex compared with other regions. In conclusion, SDs are inducible preferentially in primary sensory cortex in mice and most likely in humans. This tropism can explain the predominant sensory phenomenology of migraine aura. It also demonstrates that sensory cortices are vulnerable in brain injury. SIGNIFICANCE STATEMENT: Spreading depolarizations (SDs) are involved in neurologic disorders as diverse as migraine and traumatic brain injury. In migraine, the nature of aura symptoms suggests that sensory cortex may be preferentially susceptible. In brain injury, SDs occur at a vulnerable time, during which the issue of sensory stimulation is much debated. We show, in mouse and human, that sensory cortex is more susceptible to SDs. We find that sensory stimulation biases the timing but not the location of the depolarizations. Finally, we show a relative impairment of potassium clearance in sensory cortex, providing a potential mechanism for the susceptibility. Our data help to explain the sensory nature of the migraine aura and reveal that sensory cortices are vulnerable in brain injury.

Effects of Nozzle Geometry on the Fluid Dynamics of Thin Liquid Films Flowing down Vertical Strings in the Rayleigh-Plateau Regime.

Thin-liquid films flowing down vertical strings undergo instability, creating wavy film profiles and traveling beads. Previous studies assumed that the liquid film thickness and velocity profiles within the healing length from a nozzle were specified by the Nusselt solution, independent of the nozzle geometry. As a result, the influence of the nozzle diameter on the flow characteristics, such as the liquid bead size, spacing, and traveling speed, was largely overlooked. We report an experimental and numerical simulation study on liquid-film flows in the Rayleigh-Plateau regime while systematically varying the nozzle diameter from 0.5 to 3.2 mm at different mass flow rates (0.02, 0.04, 0.06, and 0.08 g/s). We find that the nozzle diameter does have a strong influence on the flow regime and the flow characteristics. We identify the thickness of a nearly flat portion of a liquid film that precedes the onset of instability, which we term the preinstability thickness, as a critical flow parameter that governs the size, spacing, and frequency of liquid beads that develop downstream. By defining the liquid film aspect ratio α in terms of the preinstability thickness, we capture a flow transition from the Rayleigh-Plateau (RP) instability regime to the isolated droplet regime. Improved understanding of the flow regimes and characteristics assists in the systematic design and optimization of a wide variety of processes and devices, including fiber coating and direct contact heat and mass exchangers.

A Combined Experimental and Numerical Modeling Study of the Deformation and Rupture of Axisymmetric Liquid Bridges under Coaxial Stretching.

The deformation and rupture of axisymmetric liquid bridges being stretched between two fully wetted coaxial disks are studied experimentally and theoretically. We numerically solve the time-dependent Navier-Stokes equations while tracking the deformation of the liquid-air interface using the arbitrary Lagrangian-Eulerian (ALE) moving mesh method to fully account for the effects of inertia and viscous forces on bridge dynamics. The effects of the stretching velocity, liquid properties, and liquid volume on the dynamics of liquid bridges are systematically investigated to provide direct experimental validation of our numerical model for stretching velocities as high as 3 m/s. The Ohnesorge number (Oh) of liquid bridges is a primary factor governing the dynamics of liquid bridge rupture, especially the dependence of the rupture distance on the stretching velocity. The rupture distance generally increases with the stretching velocity, far in excess of the static stability limit. For bridges with low Ohnesorge numbers, however, the rupture distance stay nearly constant or decreases with the stretching velocity within certain velocity windows due to the relative rupture position switching and the thread shape change. Our work provides an experimentally validated modeling approach and experimental data to help establish foundation for systematic further studies and applications of liquid bridges.

Minimum conditions for the induction of cortical spreading depression in brain slices.

Cortical spreading depression (CSD) occurs during various forms of brain injury such as stroke, subarachnoid hemorrhage, and brain trauma, but it is also thought to be the mechanism of the migraine aura. It is therefore expected to occur over a range of conditions including the awake behaving state. Yet it is unclear how such a massive depolarization could occur under relatively benign conditions. Using a microfluidic device with focal stimulation capability in a mouse brain slice model, we varied extracellular potassium concentration as well as the area exposed to increased extracellular potassium to determine the minimum conditions necessary to elicit CSD. Importantly, we focused on potassium levels that are physiologically plausible (≤145 mM; the intracellular potassium concentration). We found a strong correlation between the threshold concentration and the slice area exposed to increased extracellular potassium: minimum area of exposure was needed with the highest potassium concentration, while larger areas were needed at lower concentrations. We also found that moderate elevations of extracellular potassium were able to elicit CSD in relatively small estimated tissue volumes that might be activated under noninjury conditions. Our results thus show that CSD may be inducible under the conditions that expected in migraine aura as well as those related to brain trauma.

Cortical sensory plasticity in a model of migraine with aura.

The migraine attack is characterized by alterations in sensory perception, such as photophobia or allodynia, which have in common an uncomfortable amplification of the percept. It is not known how these changes arise. We evaluated the ability of cortical spreading depression (CSD), the proposed mechanism of the migraine aura, to shape the cortical activity that underlies sensory perception. We measured forepaw- and hindpaw-evoked sensory responses in rat, before and after CSD, using multielectrode array recordings and two-dimensional optical spectroscopy. CSD significantly altered cortical sensory processing on a timescale compatible with the duration of the migraine attack. Both electrophysiological and hemodynamic maps had a reduced surface area (were sharpened) after CSD. Electrophysiological responses were potentiated at the receptive field center but suppressed in surround regions. Finally, the normal adaptation of sensory-evoked responses was attenuated at the receptive field center. In summary, we show that CSD induces changes in the evoked cortical response that are consistent with known mechanisms of cortical plasticity. These mechanisms provide a novel neurobiological substrate to explain the sensory alterations of the migraine attack.

Thermal management and control of wearable devices.

The emergence of wearable devices over the recent decades has motivated numerous studies aimed at developing flexible or stretchable materials and structures for their electronic or optoelectronic functionalities. Like in conventional devices, electronic and optoelectronic components in wearable devices must be kept within certain temperature ranges to ensure reliability, performance, and/or functionality. But this must be accomplished without requiring any bulky heat sinks or other heat transfer augmentation elements. At the same time, the proximity of wearable devices to the human skin poses additional requirements of thermal comfort and safety. A growing body of literature is now focusing on the thermal management or control of wearable devices and related development of new materials and structures. The present article aims to provide a broad overview of such materials and structures and offer suggestions for future research directions.

Desalinating a real hyper-saline pre-treated produced water via direct-heat vacuum membrane distillation.

Membrane distillation (MD) is an emerging thermal desalination technology capable of desalinating waters of any salinity. During typical MD processes, the saline feedwater is heated and acts as the thermal energy carrier; however, temperature polarization (as well as thermal energy loss) contributes to low distillate fluxes, low single-pass water recovery and poor thermal efficiency. An alternative approach is to integrate an extra thermal energy carrier as part of the membrane and/or module assembly, which can channel externally provided heat directly to the membrane-feedwater interface and/or along the feed channel length. This direct-heat delivery has been demonstrated to increase single-pass water recovery and enhance the overall thermal efficiency. We developed a bench-scale direct-heated vacuum MD (DHVMD) process to desalinate pre-treated oil and gas "produced water" with an initial total dissolved solids of 115,500 ppm at a feed temperature ranging between 24 and 32 °C. We evaluated both water flux and specific energy consumption (SEC) as a function of water recovery. The system achieved a 50% water recovery without significant scaling, with an average flux >6 kg m-2 hr-1 and a SEC as low as 2,530 kJ kg-1. The major species of mineral scales (i.e., NaCl, CaSO4, and SrSO4) that limited the water recovery to 68% were modeled in terms of thermodynamics and identified by scanning electron microscopy and energy-dispersive X-ray spectroscopy. In addition, we further developed and employed a physics-based process model to estimate temperature, salinity, water transport and energy flows for full-scale vacuum MD and DHVMD modules. Model results show that a direct-heat input rate of 3,600 W can increase single-pass water recovery from 2.1% to 3.1% while lowering the thermal SEC from 7,800 kJ kg-1 to 6,517 kJ kg-1 in an unoptimized module. Finally, the scaling up potential of DHVMD process is briefly discussed.

Experimental study of a string-based counterflow wet electrostatic precipitator for collection of fine and ultrafine particles.

Wet electrostatic precipitators (WESP) have been widely studied for collecting fine and ultrafine particles, such as diesel particulate matter (DPM), which have deleterious effects on human health. Here, we report an experimental and numerical simulation study on a novel string-based two-stage WESP. Our new design incorporates grounded vertically aligned polymer strings, along which thin films of water flow down. The water beads, generated by intrinsic flow instability, travel down the strings and collect charged particles in the counterflowing gas stream. We performed experiments using two different geometric configurations of WESP: rectangular and cylindrical. We examined the effects of the WESP electrode bias voltage, air stream velocity, and water flow rate on the number-based fractional collection efficiency for particles of diameters ranging from 10 nm to 2.5 µm. The collection efficiency improves with increasing bias voltages or decreasing airflow rates. At liquid-to-gas (L/G) as low as approximately 0.0066, our design delivers a collection efficiency over 70% even for fine and ultrafine particles. The rectangular and cylindrical configurations exhibit similar collection efficiencies under nominally identical experimental conditions. We also compare the water-to-air mass flow rate ratio, air flow rate per unit collector volume, and collection efficiency of our string-based design with those of previously reported WESPs. The present work demonstrates a promising design for a highly efficient, compact, and scalable two-stage WESPs with minimal water consumption.Implications: Wet Electrostatic Precipitators (WESPs) are highly effective for collecting fine particles in exhaust air streams from various sources such as diesel engines, power plants, and oil refineries. However, their large-scale adoption has been limited by high water usage and reduced collection efficiencies for ultrafine particles. We perform experimental and numerical investigation to characterize the collection efficiency and water flow rate-dependence of a new design of WESP. The string-based counterflow WESP reported in this study offers number-based collection efficiencies >70% at air flow rates per collector volume as high as 4.36 (m3/s)/m3 for particles of diameters ranging from 10 nm - 2.5 µm, while significantly reducing water usage. Our work provides a basis for the design of more compact and water-efficient WESPs.

Finger-Powered Electro-Digital-Microfluidics.

Portable microfluidic devices are promising for point-of-care (POC) diagnosis and bio- and environmental surveillance in resource-constrained or non-laboratory environments. Lateral-flow devices, some built off paper or strings, have been widely developed but the fixed layouts of their underlying wicking/microchannel structures limit their flexibility and present challenges in implementing multistep reactions. Digital microfluidics can circumvent these difficulties by addressing discrete droplets individually. Existing approaches to digital microfluidics, however, often require bulky power supplies/batteries and high voltage circuits. We present a scheme to drive digital microfluidic devices by converting mechanical energy of human fingers to electrical energy using an array of piezoelectric elements. We describe the integration our scheme into two promising digital microfluidics platforms: one based on the electro-wetting-on-dielectric (EWOD) phenomenon and the other on the electrophoretic control of droplet (EPD). Basic operations of droplet manipulations, such as droplet transport, merging and splitting, are demonstrated using the finger-powered digital-microfluidics.

Heterogeneous incidence and propagation of spreading depolarizations.

Spreading depolarizations are implicated in a diverse set of neurologic diseases. They are unusual forms of nervous system activity in that they propagate very slowly and approximately concentrically, apparently not respecting the anatomic, synaptic, functional, or vascular architecture of the brain. However, there is evidence that spreading depolarizations are not truly concentric, isotropic, or homogeneous, either in space or in time. Here we present evidence from KCl-induced spreading depolarizations, in mouse and rat, in vivo and in vitro, showing the great variability that these depolarizations can exhibit. This variability can help inform the mechanistic understanding of spreading depolarizations, and it has implications for their phenomenology in neurologic disease.

EWOD (electrowetting on dielectric) digital microfluidics powered by finger actuation.

We report finger-actuated digital microfluidics (F-DMF) based on the manipulation of discrete droplets via the electrowetting on dielectric (EWOD) phenomenon. Instead of requiring an external power supply, our F-DMF uses piezoelectric elements to convert mechanical energy produced by human fingers to electric voltage pulses for droplet actuation. Voltage outputs of over 40 V are provided by single piezoelectric elements, which is necessary for oil-free EWOD devices with thin (typically <1 µm) dielectric layers. Higher actuation voltages can be provided using multiple piezoelectric elements connected in series when needed. Using this energy conversion scheme, we confirmed basic modes of EWOD droplet operation, such as droplet transport, splitting and merging. Using two piezoelectric elements in series, we also successfully demonstrated applications of F-DMF for glucose detection and immunoassay. Not requiring power sources, F-DMF offers intriguing paths for various portable and other microfluidic applications.

Development and characterization of a microfluidic chamber incorporating fluid ports with active suction for localized chemical stimulation of brain slices.

We report a novel microfluidic chamber incorporating fluid ports with active suction to achieve localized chemical stimulation of brain slices. A two-level soft-lithography process is used to fabricate fluid ports with integrated injection and suction holes that are connected to underlying microchannels. Fluorescence imaging, particle tracking velocimetry, and cell staining are used to characterize flows around a fluid port with or without active suction to validate effective localization of injected chemicals. To demonstrate biological applicability of the chamber, we show an induction of cortical spreading depression (CSD) waves in mouse brain slices through controlled focal delivery of potassium chloride solution.