Functional Analysis of Vibrio vulnificus Orthologs of Escherichia coli RraA and RNase E.

RNase E plays an important role in the degradation and processing of RNA in Escherichia coli. The enzymatic activity of RNase E is controlled by the protein inhibitors RraA and RraB. The marine pathogenic bacterium Vibrio vulnificus also contains homologs of RNase E and RraA, designated as RNase EV, RraAV1, and RraAV2. Here, we report that RraAV1 actively inhibits the enzymatic activity of RNase EV in vivo and in vitro by interacting with the C-terminal domain of RNase EV. Coexpression of RraAV1 reduced ribonucleolytic activity in the cells overproducing RNase EV and consequently restored normal growth of these cells. An in vitro cleavage assay further demonstrated that RraAV1 efficiently inhibits the ribonucleolytic activity of RNase EV on BR10 + hpT, a synthetic oligonucleotide containing the RNase E cleavage site of RNA I. Our findings suggest that RraAV1 plays an active role in RNase EV-mediated RNA cleavage in V. vulnificus.

Effects of country animosity of angry Koreans on Japan: A focus on export regulation on Korea.

Nowadays, Korea and Japan are in conflict arising from export restrictions launched by Japan on Korea, which have provoked a boycott of Japanese products in Korea, and even tourism to Japan. Animosity performs a momentous role in the context of crisis management communication. Hence, this article aims to investigate factors impacting boycott intention to visit Japan, with economic animosity being a principal mediating variable, whose antecedents and consequences have been probed into. A total of 333 respondents' survey data were collected and analyzed via SEM for the verification of research hypotheses. The findings manifest that ethnic identity engenders significant direct positive bearings upon economic animosity and boycott news, and boycott news significantly positively affects economic animosity; boycott news serves as the mediating role between ethnic identity and economic animosity. Additionally, the outcomes denote that economic animosity exerts a significant positive impact on boycott visit intention, economic animosity negatively affects Japanese government trust, and Japanese government trust negatively bears upon boycott visit intention; Japanese government trust mediates between economic animosity and boycott intention to visit Japan. Consequently, the research makes contributions to furnishing empirical evidence for influencing factors of boycott visit intention and enriching the literature on the antecedents and consequences of animosity.

Role of gambling media exposure in influencing trajectories among college students.

In the study, a model of the relationship between mass media depictions of gambling and subsequent gambling attitudes and behavioral intentions of college students was developed. A survey was conducted with 229 undergraduate students (79.5% female, mean age = 20.5, SD = 2.04) enrolled in three different communication courses at a large southeastern university. Through structural equation analysis, the six hypotheses of this study were analyzed using the method of maximum likelihood with AMOS 6. The model was consistent with the hypotheses that media exposure impacts gambling attitudes and behavioral intentions both positively and negatively, depending on the valence of the gambling depiction. Theoretical and practical implications of mass media exposure and gambling behaviors are discussed, and future research directions are outlined.

Torso, a Drosophila receptor tyrosine kinase, plays a novel role in the larval fat body in regulating insulin signaling and body growth.

Torso is a receptor tyrosine kinase whose localized activation at the termini of the Drosophila embryo is mediated by its ligand, Trunk. Recent studies have unveiled a second function of Torso in the larval prothoracic gland (PG) as the receptor for the prothoracicotropic hormone, which triggers pupariation. As such, inhibition of Torso in the PG prolongs the larval growth period, thereby increasing the final pupa size. Here, we report that Torso also acts in the larval fat body, regulating body size in a manner opposite from that of Torso in PG. We confirmed the expression of torso mRNA in the larval fat body and its reduction by RNA interference (RNAi). Fat body-specific knockdown of torso, by either of the two independent RNAi transgenes, significantly decreased the final pupal size. We found that torso knockdown suppresses insulin/target of rapamycin (TOR) signaling in the fat body, as confirmed by repression of Akt and S6K. Notably, the decrease in insulin/TOR signaling and decrease of pupal size induced by the knockdown of torso were rescued by the expression of a constitutively active form of the insulin receptor or by the knockdown of FOXO. Our study revealed a novel role for Torso in the fat body with respect to regulation of insulin/TOR signaling and body size. This finding exemplifies the contrasting effects of the same gene expressed in two different organs on organismal physiology.