RESUMO
Pulmonary administration of drugs has recently gained attention because it exhibits numerous advantages compared to oral or intravenous administration. The administration of aerosols for inhalation to animals, however, remains a critical challenge and only a few methods of administration have been developed. Herein, we compare the regional distribution of aerosols in the lungs of wild type rats after pulmonary administration using three different methods: (a) The Penn-Century MicroSprayer® Aerosolizer; (b) an in-house designed aerosol generation system; and (c) the Aeroneb® Lab Micropump Nebulizer. Both the regional distribution and the fraction of aerosol deposited in the lungs were determined by means of radiolabelling of the aerosol followed by in vivo and ex vivo Positron Emission Tomography (PET) imaging and dissection and gamma counting. Endotracheal insufflation using the PennCentury MicroSprayer resulted in >85% of the administered dose accumulated in the lungs, with a non-uniform distribution of the radioactivity in different lobes and a low animal-to-animal reproducibility. Administration using the in-house designed and the Aeroneb nebulizers resulted in a uniform distribution over the lungs, but only a small fraction of the nebulized activity (ca. 0.1%) was deposited in the lungs.
Assuntos
Aerossóis , Pulmão/metabolismo , Tomografia por Emissão de Pósitrons/métodos , Compostos Radiofarmacêuticos/administração & dosagem , Administração por Inalação , Animais , Feminino , Nebulizadores e Vaporizadores , Preparações Farmacêuticas/administração & dosagem , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Distribuição TecidualRESUMO
El cuidado debebés prematuros y de sus padres ha sido la razón de ser del programa Madre-Bebé-Canguro. En este artículoqueremos visibilizar este empeño desde que se creó en Colombia y destacar algunos de los difíciles estadosemocionales del equipo de salud que atiende este servicio, en medio de la COVID-19, en condiciones de intensasensibilidad y peligro real. Enfrentar la incertidumbre, el riesgo de muerte, en escenarios tan vulnerables comola prematuridad, promueve la creatividad humana y la dedicación prioritaria al cuidado emocional y físico. Elsufrimiento emocional que abarcó tantas esferas en la cotidianidad y sus respuestas creativas hicieron de estasexperiencias modelos de abordajes sensibles y amorosos para cuidar la vida, en toda su extensión.(AU)
Caring for prematurebabies and their parents has been the fundemental purpose of the Mother-Baby Kangaroo Programme. Since it wascreated in Colombia, we want to make this endeavour visible and highlight some of the difficult emotional states of thehealth team that attends to this service, in the midst of COVID-19, in conditions of intense sensitivity and real danger.Facing uncertainty, the risk of death, in scenarios as vulnerable as prematurity, promotes human creativity and prioritydedication to emotional and physical care. The emotional suffering that encompassed so many spheres in everyday lifeand their creative responses made these experiences models of sensitive and loving approaches to caring for life in itsfullest extent.(AU)
La cura dels nadons pre-maturs i dels seus pares ha estat la raó de ser del programa mare-nadó-cangur. En aquest article volem visibilitzaraquest esforç des que es va crear a Colòmbia i ressaltar alguns dels difícils estats emocionals de lequip de salut queatén aquest servei, enmig de la COVID-19, en condicions de sensibilitat intensa i perill real. Enfrontar la incertesa, el riscde mort, en escenaris tan vulnerables com la prematuritat, promou la creativitat humana i la dedicació prioritària a lacura emocional i física. El patiment emocional que va recórrer tantes esferes a la vida quotidiana i les seves respostescreatives van fer daquestes experiències models dabordatge sensibles i amorosos per a la cura de la vida, en tota laseva extensió.(AU)