RESUMO
It has previously been shown that phenylarsine oxide (PhAsO), an inhibitor of protein internalization, also inhibits stereospecific uptake of D-glucose and 2-deoxyglucose in both basal and insulin-stimulated rat adipocytes. This inhibition of hexose uptake was found to be dose-dependent. PhAsO rapidly inhibited sugar transport into insulin-stimulated adipocytes, but at low concentrations inhibition was transient. Low doses of PhAsO (1 microM) transiently inhibit stereospecific hexose uptake and near total (approx. 90%) recovery of transport activity occurs within 20 min. Interestingly, once recovered, the adipocytes can again undergo rapid inhibition and recovery of transport activity upon further treatment with PhAsO (1 microM). In addition, PhAsO is shown to inhibit cytochalasin B binding to plasma membranes from insulin-stimulated adipocytes in a concentration-dependent manner which parallels the dose-response inhibition of hexose transport by PhAsO. The data presented suggest a direct interaction between the D-glucose transporter and PhAsO, resulting in inhibition of transport. The results are consistent with the current recruitment hypothesis of insulin activation of sugar transport and indicate that a considerable reserve of intracellular glucose carriers exists within fat cells.
Assuntos
Tecido Adiposo/efeitos dos fármacos , Arsenicais/farmacologia , Glucose/metabolismo , Proteínas de Transporte de Monossacarídeos/antagonistas & inibidores , Tecido Adiposo/metabolismo , Animais , Ligação Competitiva , Transporte Biológico/efeitos dos fármacos , Citocalasina B/antagonistas & inibidores , Camundongos , Ratos , Estereoisomerismo , Fatores de TempoAssuntos
Papillomaviridae , Infecções por Papillomavirus/virologia , Neoplasias do Colo do Útero/virologia , DNA Viral/análise , DNA Viral/genética , Feminino , Papillomavirus Humano 16/genética , Papillomavirus Humano 6/genética , Humanos , Papillomaviridae/genética , Infecções por Papillomavirus/epidemiologia , Infecções por Papillomavirus/terapia , Reação em Cadeia da Polimerase , Prevalência , Fatores de Risco , Trabalho Sexual , Tunísia/epidemiologia , Neoplasias do Colo do Útero/epidemiologia , Neoplasias do Colo do Útero/terapiaRESUMO
Uterine rupture was seen in a total of 1.1 per cent of 10,012 pregnancies involving scarred uteri. Rupture frequency during pregnancy was estimated as 0.4 per cent, although this is doubtless an underestimate due to the occasionally asymptomatic nature of the condition. Maternal mortality was nil. Fetal mortality related to uterine rupture was also nil, but psychomotor backwardness was seen in one case of uterine rupture after completion of 30 weeks of pregnancy. These results and those of the literature show that maternal and fetal risks linked to uterine rupture are significant during pregnancy and very small during confinement. The indications for systematic prophylactic cesarean section are discussed. An increased frequency of uterine tests could decrease the overall rate of cesarean sections without danger to mother or baby.