RESUMO
AIM: To study the characteristics of health state in family-members of ENT-patients with identified Chlamydia infection. METHODS: We have examined 245 members of 87 families of otorhinolaryngological patients with verified (44 families) and without (44 families) Chlamydia infection. Laboratory methods of the research included the identification of the following types: Chlamydia trachomatis and Chlamydophila pneumoniae. Chlamydia diagnostics involved both direct immune fluorescence and immune enzyme analyses as well as polymerase chain reaction. Health state in ENT-patient family-members has been evaluated by the complex method based on the special questionnaire for every family-member aimed at determining the health category, the calculation of health average coefficient for each family member followed by the determination of family identity with some definite health category. RESULTS: in the families of otorhinolaryngological patients with verified Chlamydia infection there have been revealed considerably more family-members with acute and chronic pathology of different organs and systems associated with functional disorders. The health status of family members of ENT patients with respiratory Chlamydia was significantly worse than in families of patients with negative laboratory results for Chlamydia, which was manifested by a much smaller number of healthy family members and a large number of people with risk factors for health.
Assuntos
Infecções por Chlamydia , Chlamydophila pneumoniae , Chlamydia trachomatis , Família , Saúde da Família , Humanos , Fatores de RiscoRESUMO
The present study included 201 adult patients presenting with exacerbation of chronic maxillary sinusitis. The presence of Chlamydia trachomatis and Chl. pneumoniae was verified by the direct immunofluorescencetechnique and polymerase chain reaction. The study material consisted of swipes und swabs from the mucous membrane of the middle nasal passage. The information from the patients was collected with the use of a questionnaire specially elaborated for the purpose of this study. The correlation relationships were established by means of gamma-statistics. The method is based on the calculation of the integral index characterizing the risk of development of chlamydial infection using the scoring scale for the evaluation of the clinical and anamnestic characteristics of the patients. The assessment of the risk of chlamydial colonization by the anamnestic method makes it possible to enhance the effectiveness of clinical diagnostics of chlamydial infection and thereby provides a basis for the prescription of the adequate anti-chlamydial treatment facilitating reduction of the frequency of complications and preventing dissemination of the causative factor of the disease. Moreover, this approach creates the conditions for the targeted selection of the patients to be referred to the laboratory verification of Chlamydia. Highoperating performance and effectiveness characteristics of the clinic-anamnestic diagnostics make it a method of choice for the wide application in the clinical practice.
Assuntos
Antibacterianos/uso terapêutico , Infecções por Chlamydia , Chlamydia trachomatis , Chlamydophila pneumoniae , Sinusite Maxilar , Adulto , Técnicas Bacteriológicas/métodos , Infecções por Chlamydia/complicações , Infecções por Chlamydia/diagnóstico , Infecções por Chlamydia/tratamento farmacológico , Infecções por Chlamydia/microbiologia , Chlamydia trachomatis/efeitos dos fármacos , Chlamydia trachomatis/isolamento & purificação , Chlamydophila pneumoniae/efeitos dos fármacos , Feminino , Humanos , Masculino , Sinusite Maxilar/diagnóstico , Sinusite Maxilar/tratamento farmacológico , Sinusite Maxilar/microbiologia , Sinusite Maxilar/fisiopatologia , Testes de Sensibilidade Microbiana/métodos , Pessoa de Meia-Idade , Medição de Risco , Prevenção Secundária , Resultado do TratamentoRESUMO
The objective of the present study was to develop a system of laboratory diagnostics ofchlamydial infection in the mucous membrane of the nose and paranasal sinuses in the patients presenting with chronic sinusitis. A total of 168 subjects at the age varying from 18 to 65 years were available for the examination based at the Department of Otorhinolaryngologyduring the period of exacerbation of chronic sinusitis. Chlamydia were identified by simultaneously using two direct (polymerase chain reaction and direct immunofluorescence) and one indirect (immunoenzymatic assay) methods. The study has demonstrated the high degree of probability of the detection of chlamydial infection based on the above complex of the laboratory methods. It is concluded that the use of the proposed algorithm in practical medicine makes it possible to improve the quality of laboratory verification of chlamydial infection and thereby to choose the adequate diagnostic strategy for the patients suspected of having chlamydial infection and to evaluate the effectiveness of the therapeutic measures being taken.
RESUMO
Medical and social state of health in family persons was assessed on the basis of a questionnaire. Developed by the author's questionnaire included two sections, reflecting the state of health, health risk factors and social characteristics of the family members of ENT patients. In the article there is presented an analysis of the medical and social state of 44 families of patients with diseases of the upper respiratory tract associated with chlamydial infection. The comparison was performed with 43 families of ENT patients with unconfirmed respiratory chlamydia. Diagnosis of Chlamydia infection complex was carried out with the use of laboratory methods (direct immunofluorescence and enzyme-linked immunosorbent assays, polymerase chain reaction). The health and social status of families with hearth respiratory chlamydia were shown to be significantly worse compared with families with the lack of the latter
Assuntos
Infecções por Chlamydia/epidemiologia , Família , Infecções Respiratórias/epidemiologia , Adulto , Criança , Infecções por Chlamydia/etiologia , Infecções por Chlamydia/microbiologia , Controle de Doenças Transmissíveis , Humanos , Incidência , Otorrinolaringopatias/complicações , Otorrinolaringopatias/epidemiologia , Infecções Respiratórias/etiologia , Infecções Respiratórias/microbiologia , Fatores de Risco , Federação Russa/epidemiologia , Fatores SocioeconômicosRESUMO
AIM: To explore the detection rate of chlamydia infection and immunological features of children with acute maxillary sinusitis. PATIENTS AND METHODS: Clinical epidemiologic characteristics of the course of acute maxillary sinusitis were studied in the way of medical examination provided for 58 children in the ages from 3 to 15 years, patients of ENT department. In order to identify Chlamydia structures and specific antibodies we used direct (polymerase-chain reaction, direct immune fluorescence) and indirect (immune enzyme analysis) methods. The research for cellular link of immunity was carried out by indirect immune fluorescence technique, using monoclonal antibodies. RESULTS: The authors report on high level of Chlamydia infection in epithelium of nasal mucosa, and also type structure of verified Chlamydia, clinical signs and peculiarities of immune response in children with acute maxillary sinusitis associated with this infection. CONCLUSIONS: Chlamydia was detected in 48% of children with acute maxillary sinusitis infection with prevalence of Chlamydophila pneumoniae. Optimal for detection of chlamydia infection is a disease duration not exceeding 2 weeks. The special features of clinical and immunological manifestations in children with acute sinusitis, paired with Chlamydia infection are the more frequent complaints of pain in the affected sinuses, expressed symptoms of intoxication, and the presence of an imbalance in cellular immunity, manifested a relative lymphopenia and activation of T-lymphocytes CD8+.
Assuntos
Antibacterianos/uso terapêutico , Infecções por Chlamydia/microbiologia , Chlamydia/isolamento & purificação , Sinusite Maxilar/microbiologia , Doença Aguda , Adolescente , Criança , Pré-Escolar , Infecções por Chlamydia/tratamento farmacológico , Infecções por Chlamydia/epidemiologia , Feminino , Seguimentos , Humanos , Incidência , Masculino , Sinusite Maxilar/tratamento farmacológico , Sinusite Maxilar/epidemiologia , Sibéria/epidemiologiaRESUMO
The objective of the present study was to evaluate the medico-social status of 44 families having patients with ENT pathology; special attention was given to the patients with this condition and concomitant verified Chlamydia infection in the upper respiratory tract. The results of their examination were compared with those obtained in the control group of 43 families including the patients with ENT pathology and unconfirmed respiratory chlamydiasis. Chlamydia infection was diagnosed by combining standard laboratory techniques such as direct immunofluorescent and immunoenzymatic assays alomg with polymerase chain reaction. The results of the present study indicate that the medical and social status of the families having a member with respiratory chlamydiasis is inferior to that of the families unaffected by this infection.
Assuntos
Infecções por Chlamydia/epidemiologia , Chlamydophila pneumoniae/isolamento & purificação , Família , Infecções Respiratórias/epidemiologia , Adulto , Criança , Pré-Escolar , Infecções por Chlamydia/diagnóstico , Infecções por Chlamydia/microbiologia , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Mucosa Respiratória/microbiologia , Infecções Respiratórias/diagnóstico , Infecções Respiratórias/microbiologia , Fatores de Risco , Sibéria/epidemiologia , Fatores SocioeconômicosRESUMO
The objective of the present study was to estimate the prevalence and specific features of chlamydial infection within an organized group of children of pre-school age. The data concerning the frequency of chlamydial infection of the upper respiratory tract in the children attending pre-school facilities are reported. The species composition of identified Chlamydia depending on the sex of the children is described. It is shown that acute and chronic ENT pathology in the patients presenting with chlamydial infection occurs much more frequently than in the unaffected children. These results were obtained during the examination of 121 children. Chlamydia was identified by means of the direct immunofluorescence technique.
Assuntos
Infecções por Chlamydia/epidemiologia , Chlamydia trachomatis/isolamento & purificação , Mucosa Respiratória/microbiologia , Infecções Respiratórias/epidemiologia , Criança , Pré-Escolar , Infecções por Chlamydia/diagnóstico , Infecções por Chlamydia/microbiologia , Feminino , Humanos , Masculino , Prevalência , Infecções Respiratórias/diagnóstico , Infecções Respiratórias/microbiologia , Sibéria/epidemiologiaRESUMO
The objective of the present study was to elaborate the complex of measures intended to optimize medical care for the patients suffering respiratory chlamydiosis. The adequate organizational measures concerning the prophylactic and therapeutic treatment of inflammatory pathology of the upper respiratory tract associated with Chlamydia infection have not been proposed up to the present time and need to be urgently developed. The importance of this issue has been confirmed in a study involving 245 subjects that demonstrated the imperfection of the structure of the currently operating system of outpatient and hospital-based service provided to the patients with respiratory chlamydiosis. The authors propose the program that defines priority activities in the field under discussion and outline possible ways to reduce morbidity and the number of cases.
Assuntos
Infecções por Chlamydia , Chlamydia/patogenicidade , Atenção à Saúde/normas , Gerenciamento Clínico , Infecções Respiratórias , Adolescente , Adulto , Criança , Pré-Escolar , Infecções por Chlamydia/diagnóstico , Infecções por Chlamydia/epidemiologia , Infecções por Chlamydia/terapia , Atenção à Saúde/métodos , Humanos , Pessoa de Meia-Idade , Infecções Respiratórias/epidemiologia , Infecções Respiratórias/microbiologia , Infecções Respiratórias/terapia , Fatores de Risco , Adulto JovemRESUMO
At present the issues in regard to Chlamydia infection are not only limited by urogenital system. By the way optimal organization and non-urogenital chlamydiosis treatment strategy (with respiratory tract involvement in particular) have not been worked out yet and require immediate solutions. Due to new knowledge on respiratory chlamidiosis the authors discuss scientific background for future development of complex measures and main directions of health care support strategy for patients with upper respiratory associated with Chlamydia infection.
Assuntos
Infecções por Chlamydia , Chlamydia/isolamento & purificação , Técnicas de Laboratório Clínico/métodos , Necessidades e Demandas de Serviços de Saúde , Doenças Respiratórias , Adulto , Pré-Escolar , Infecções por Chlamydia/diagnóstico , Infecções por Chlamydia/epidemiologia , Infecções por Chlamydia/microbiologia , Infecções por Chlamydia/terapia , Técnicas de Laboratório Clínico/normas , Feminino , Apoio ao Planejamento em Saúde/organização & administração , Prioridades em Saúde , Humanos , Masculino , Avaliação das Necessidades/organização & administração , Melhoria de Qualidade , Doenças Respiratórias/diagnóstico , Doenças Respiratórias/epidemiologia , Doenças Respiratórias/microbiologia , Doenças Respiratórias/terapia , Fatores de Risco , Federação Russa/epidemiologiaRESUMO
The objective of this study was to elucidate specific distribution patterns and manifestations of respiratory chlamydiosis affecting the upper respiratory tract in different groups of the patients (both children and adults) presenting with otorhinolaryngological problems. Chlamydia were detected with the use of a combination of laboratory methods including PCR, direct immunofluorescent and imunoenzymatic assays. The study included 1329 patients. The data obtained suggest the high prevalence of chlamydial infection of the upper respiratory tract in different groups of the population. The species composition of the identified bacteria was shown to depend on the age and sex of the affected subjects.
Assuntos
Infecções por Chlamydia/epidemiologia , Chlamydophila pneumoniae/isolamento & purificação , Infecções Respiratórias , Adolescente , Pré-Escolar , Técnicas de Laboratório Clínico/métodos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Sistema Respiratório/microbiologia , Infecções Respiratórias/epidemiologia , Infecções Respiratórias/microbiologia , Sibéria/epidemiologiaRESUMO
The aim of the present study was to elucidate the frequency of chlamydial infection in the members of the families having the confirmed disease and concomitant ENT pathologies. Another objective was the comparative analysis of otorhinolaryngological morbidity in the families with and without the carriers of respiratory chlamydiasis. A total of 87 families were involved in the study. Chlamydial infection was diagnosed by a set of laboratory methods including direct immunofluoresent and immunoenzyme analysis as well as polymerase chain reaction (PVR). It was shown that the presence of a patient suffering from respiratory chlamydiasis in the family creates the risk of infection for its remaining members. Moreover the frequency of ENT morbidity increases in the families that serve as a hotbed of chlamydiasis of the upper respiratory tract.
Assuntos
Infecções por Chlamydia , Chlamydia trachomatis , Chlamydophila pneumoniae , Transmissão de Doença Infecciosa/prevenção & controle , Infecções Respiratórias , Adulto , Criança , Infecções por Chlamydia/epidemiologia , Infecções por Chlamydia/fisiopatologia , Infecções por Chlamydia/prevenção & controle , Chlamydia trachomatis/isolamento & purificação , Chlamydia trachomatis/patogenicidade , Chlamydophila pneumoniae/isolamento & purificação , Chlamydophila pneumoniae/patogenicidade , Controle de Doenças Transmissíveis , Saúde da Família , Feminino , Humanos , Masculino , Otorrinolaringopatias/complicações , Otorrinolaringopatias/fisiopatologia , Sistema Respiratório/microbiologia , Infecções Respiratórias/complicações , Infecções Respiratórias/epidemiologia , Infecções Respiratórias/microbiologia , Infecções Respiratórias/fisiopatologia , Fatores de Risco , Sibéria/epidemiologiaRESUMO
AIM: Comparative study of microflora of nasal passages during acute and chronic inflammatory nasal diseases with and without Chlamydia infection. MATERIALS AND METHODS: Microflora of nasal mucosa obtained from 104 patients with acute maxillary sinusitis and chronic nasal diseases (sinusitis and rhinitis) with and without detected Chlamydia infection. Verification of the latter was performed by PCR and direct immunofluorescent method. Microbiological isolation was performed on blood agar using method of sectors. Identification of streptococci, bacteria from Enterobacteriaceae family, staphylococci, and bacteria from Neisseria genus was performed by conventional methods. RESULTS: During acute maxillary sinusitis associated with Chlamydia infection, depletion of bacterial species diversity was observed with increase of quantity of staphylococci. Also, increase of number of staphylococcal strains with pathogenic properties in patients with Chlamydia carriage was observed. CONCLUSION: It could be suggested that Chlamydia, pathogenic, and opportunistic bacteria are antagonists, which compete for ecologic niche and substrate in host organism. Decrease of number of staphylococci is a response on invasion and multiplication of Chlamydia on mucosa of nasal passages.
Assuntos
Bactérias/isolamento & purificação , Infecções por Chlamydia/complicações , Chlamydia trachomatis , Mucosa Nasal/microbiologia , Rinite/microbiologia , Sinusite/microbiologia , Bactérias/patogenicidade , Carga Bacteriana , Infecções por Chlamydia/diagnóstico , Doença Crônica , Humanos , Rinite/complicações , Sinusite/complicações , VirulênciaRESUMO
The present work was designed to analyse the results of a questionnaire study including 104 patients with acute and chronic pathology of nasal cavity and paranasal sinuses associated with chlamydial infection and in its absence. It is shown that either group, regardless of infection, is dominated by patients at high medical and social risk of health conditions. Respondents with confirmed chlamydial infection were found to be distinctive for the poorer health status and subject to a broader range of negative social factors.
Assuntos
Infecções por Chlamydia/epidemiologia , Chlamydia/isolamento & purificação , Mucosa Nasal/microbiologia , Seios Paranasais/microbiologia , Rinite/epidemiologia , Sinusite/epidemiologia , Adolescente , Adulto , Idoso , Infecções por Chlamydia/diagnóstico , Humanos , Incidência , Pessoa de Meia-Idade , Mucosa Nasal/patologia , Seios Paranasais/patologia , Rinite/diagnóstico , Rinite/microbiologia , Fatores de Risco , Sibéria/epidemiologia , Sinusite/diagnóstico , Sinusite/microbiologia , Fatores Socioeconômicos , Adulto JovemRESUMO
This paper presents characteristics of prevalence and structure of chronic otorhinolaringologic pathologies among native and alien urban residents of the Republic of Khakassia. A total of 2,607 subjects were selected and examined at random. Results of the cross-section study suggest high prevalence of ENT organ diseases among the examined population regardless of ethnic background. Comparative analysis of prevalence indices in the Europeoids and the Khakass revealed a number of specific differences in the epidemiology of ear, nose, and throat diseases between the two groups.
Assuntos
Etnicidade , Otorrinolaringopatias/etnologia , Migrantes , População Urbana , Adolescente , Adulto , Idoso , Doença Crônica , Humanos , Pessoa de Meia-Idade , Prevalência , Sibéria/epidemiologia , Adulto JovemRESUMO
This paper reports results of the study of nasal mucosal microflora in patients with chronic sinusitis (CS) and rhinitis (CR). The study revealed dysbacteriosis of nasal mucosa in the patients of both groups. At the same time, the structure of mucosal microbiocenoses was not significantly different in patients with CS and CR. Moreover, the study has demonstrated enhanced pathogenic activity of Staphylococcus bacteria in both CS and CR.
Assuntos
Mucosa Nasal/microbiologia , Rinite/microbiologia , Sinusite/microbiologia , Infecções Estafilocócicas/microbiologia , Staphylococcus/isolamento & purificação , Adulto , Doença Crônica , Humanos , Pessoa de Meia-Idade , Staphylococcus/patogenicidade , Adulto JovemRESUMO
Comparative analysis of results of clinical and laboratory evaluation of patients with chronic nasal or nasal sinuses' diseases (chronic rhinitis or maxillary sinusitis) associated or not associated with Chlamydia infection was performed. It was shown that in patients infected with Chlamydia, along with unidirectional changes typical for all patients irrespective from presence or absence of Chlamydia, the features of immune response against these infectious agents take place.
Assuntos
Infecções por Chlamydia/imunologia , Chlamydia/imunologia , Sinusite Maxilar/imunologia , Rinite/imunologia , Adolescente , Adulto , Anticorpos Antibacterianos/sangue , Especificidade de Anticorpos , Complexo Antígeno-Anticorpo/sangue , Complexo Antígeno-Anticorpo/imunologia , Antígenos de Bactérias/imunologia , Antígenos CD/análise , Infecções por Chlamydia/sangue , Doença Crônica , Humanos , Linfócitos/sangue , Linfócitos/imunologia , Sinusite Maxilar/microbiologia , Pessoa de Meia-Idade , Rinite/microbiologiaRESUMO
Quantitative and qualitative composition of microflora of nasal mucosa as well as carriage of staphylococci was assessed in patients with chronic and acute forms of maxillary sinusitis. Changes in microflora of nasal mucosa and presence of pathogenic and persistence-associated characteristics of staphylococci in both forms of maxillary sinusitis were revealed. Increase of resistance staphylococci to antibiotics in patients with chronic form of maxillary sinusitis was shown.
Assuntos
Sinusite Maxilar/microbiologia , Mucosa Nasal/microbiologia , Staphylococcus/classificação , Staphylococcus/isolamento & purificação , Antibacterianos/farmacologia , Técnicas de Tipagem Bacteriana , Doença Crônica , Contagem de Colônia Microbiana , Farmacorresistência Bacteriana , Humanos , Seio Maxilar/microbiologia , Staphylococcus/efeitos dos fármacosRESUMO
The examination of 169 children aged 3-15 years admitted to ENT department for exacerbation of chronic adenoiditis has shown high chlamydial contamination of the pharyngeal mucosa. Identification of chlamydial structures and specific antibodies was performed with application of direct (polymerase chain reaction, immunofluorescence) and indirect (EIA) methods. Specific structure of the verified pathogens as well as clinical manifestations of adenoiditis associated with chlamydial infection and characteristics of immune response of the child to chlamydia are described.
Assuntos
Tonsila Faríngea/microbiologia , Infecções por Chlamydia/microbiologia , Adolescente , Criança , Pré-Escolar , Infecções por Chlamydia/epidemiologia , Doença Crônica , Feminino , Humanos , Incidência , Masculino , PrevalênciaRESUMO
Analysis of microbial composition of nasal mucosa in patients with allergic rhinitis revealed marked changes in microflora compared with healthy subjects. Specifically, the overall diversity of staphylococci decreased while the abundance of S. aureus increased. The number of strains with pathogenic and persisting properties in patients with allergic rhinitis was greater than in controls.
Assuntos
Bactérias/isolamento & purificação , Infecções Bacterianas/microbiologia , Mucosa Nasal/microbiologia , Rinite Alérgica Perene/microbiologia , Sinusite/microbiologia , Adolescente , Adulto , Humanos , Pessoa de Meia-Idade , Prognóstico , Adulto JovemRESUMO
Overall quantity of microorganisms and their relative prevalence on nasal mucous membrane in patients with allergic rhinosinusitis are determined. Microbial flora in studied group was characterized qualitatively and quantitatively. Obtained results showed that there were changes of microbiocenosis of nasal mucous membrane during allergic rhinosinusitis and in characteristics of species in Staphylococcus genus.