Simultaneous dairy wastewater treatment and bioelectricity production in a new microbial fuel cell using photosynthetic Synechococcus.

Photosynthetic microbial fuel cell (PMFC) is a novel technology, which employs organic pollutants and organisms to produce electrons and biomass and capture CO2 by bio-reactions. In this study, a new PMFC was developed based on Synechococcus sp. as a biocathode, and dairy wastewater was used in the anode chamber. Different experiments including batch feed mode, semi-continuous feed mode, Synechococcus feedstock to the anode chamber, Synechococcus-Chlorella mixed system, the feedstock of treated wastewater to the cathode chamber, and use of extra nutrients in the anodic chamber were performed to investigate the behavior of the PMFC system. The results indicated that the PMFC with a semi-continuous feed mode is more effective than a batch mode for electricity generation and pollutant removal. Herein, maximum power density, chemical oxygen demand removal, and Coulombic efficiency were 6.95 mW/m2 (450 Ω internal resistance), 62.94, and 43.16%, respectively, through mixing Synechococcus sp. and Chlorella algae in the batch-fed mode. The maximum nitrate and orthophosphate removal rates were 98.83 and 68.5%, respectively, wherein treated wastewater in the anode was added to the cathode. No significant difference in Synechococcus growth rate was found between the cathodic chamber of PMFC and the control cultivation cell. The heating value of the biocathode biomass at maximum Synechococcus growth rate (adding glucose into the anode chamber) was 0.2235 MJ/Kg, indicating the cell's high ability for carbon dioxide recovery. This study investigated not only simultaneous bioelectricity production and dairy wastewater in a new PMFC using Synechococcus sp. but also studied several operational parameters and presented useful information about their effect on PMFC performance.

Exposure risk assessment, pollution level, and source identification of arsenic in soil: A case study of the Bardsir Plain (southeastern Iran).

In this study, we investigated the total arsenic concentration in the soil and the related human exposure risks in the central part of the Bardsir Plain in southeastern Iran. The results show that the average total arsenic concentration in agricultural soil is 50.26 mg/kg, which is 2.5 times higher than the maximum acceptable limit (20 mg/kg) recommended by the European Community. The natural portion of the arsenic concentration was larger than the anthropogenic portion. The high total arsenic concentration could be due to a combination of geogenic sources and irrigation with polluted groundwater. The average values of Igeo, Ipoll, and IB for agricultural soils were 1.10, 0.14, and 0.15, respectively; which are characterized as moderately polluted. The average non-carcinogenic hazard (HI) values for children and adults were 2.27 and 0.24, respectively, suggesting that children are exposed to non-carcinogenic risks. The total carcinogen risk (CR) value was 1.16E-04, which indicates a high risk of harmful effects to inhabitants.

Measurement and ecological risk assessment of heavy metals accumulated in sediment and water collected from Gomishan international wetland, Iran.

This study aimed to measure and ecologically assess heavy metals, including As, Cr, Pb, Cd, and Ni in water and sediment samples taken from Gomishan, an international wetland located in Golestan, Iran. Four sampling stations were selected to cover all parts of the wetland. The analyses of the heavy metals were performed by ICP-MS. Based on the content of the heavy metals in the sediments, the values of risks for individual heavy metals, as Er, and for total heavy metals, as IR, were estimated. Igeo and EF also presented the soil quality in terms of accumulated contamination. The average content of the heavy metals in water was 23.12, 4.14, 10.04, 6.71, and 94.48 µg/L for As, Cd, Cr, Ni, and Pb, respectively. The heavy metal concentrations in sediments were decreased in the following order: Pb (2130 ppb) > As (655 ppb) > Cr (295 ppb) > Ni (148.8 ppb) > Cd (148.8 ppb). The potential risk values for individual heavy metals were in the low range, Er < 40, except for Cd, which mostly posed a moderate ecological risk. The values of EF and Igeo showed that the sediments sampled from the Gomishan wetland were minimally enriched and contaminated. As the Gomishan wetland has a moderate risk of heavy metal contamination, conservative and monitoring activities should be performed.

The thermodynamic stability, potential toxicity, and speciation of metals and metalloids in Tehran runoff, Iran.

Surface runoff is the most significant source of water in dry cities like Tehran. The surface runoff is polluted by heavy metals, which their risk level is a function of their speciation. Herein, Tehran runoff quality and the speciation of metals and metalloids were investigated. The results of quality showed that oxidation-reduction potential (Eh) and pH ranged from + 186 to + 230 mV and from 7.31 to 10.29, respectively. Cluster analysis indicated that Cr, Si, Mn, Fe, Pb, Se, Th, Ba, Ni, Li, and Sr had similar behaviors and origins, and salinity played an active role in restricting their concentrations. Eh and dissolved oxygen (DO) negatively affected the concentrations of all the studied elements. The speciation model (according to HSC Chemistry program) exhibited that all the studied elements are stable; however, in two cases, they would become unstable (pH < 7, Eh < - 480 mV or Eh > 1100 mV) and (pH > 10, Eh < - 570 mV or Eh > 970 mV). Also, Ba, Cd, Li, Mn, Al, As, Sr, Cr, Si, and Se are present in bioavailable species and As and Cd in the runoff exist in high toxic oxidation states of + 3 and + 2, respectively. The linear regression of Cu, Co, Cd, Zn, and As with Eh provided a good fit, and all of these metals were significant at levels 1 and 5%. Finally, it is recommended to continuously monitor the Eh-pH changes for investigating the potential toxicity of metals and predicting the metal pollution by regression equations in any other stations.

Impacts of land use and land cover change on the interactions among multiple soil-dependent ecosystem services (case study: Jiroft plain, Iran).

The spatial and temporal distribution pattern is an outstanding feature of the relationship among ecosystem services (ESs) that explains links between human activities and disturbed chemical composition of ecosystems. This study investigated the spatiotemporal variation of land use/cover changes (LUCC) and quantifies the change in four essential ecosystem services with an emphasis on soil (nutrient delivery ratio, carbon storage, crop production, and water yield) and their relationships in the Jiroft plain, Iran, during 1996-2016 through analytical tools including Land Change Modeler, and the Integrated Valuation of Ecosystem Services and Tradeoff. During the 20-year concentrate period, there was a considerable overall gain in cropland (5396 km2) and urban (1787 km2), loss of unused land (5692 km2), water (2088 km2), and forest (1083 km2). As a result of LUCC, while crop production and nutrient delivery ratio showed a rising trend, overall carbon storage and water yield decreased. The spatiotemporal trade-off between carbon storage and crop production, the temporal trade-off between crop production and water yield, and synergy between water yield and crop production were widespread in Jiroft plain. These results showed that the interaction among ESs mutates over time and can be changed under planning and policies. This study will enrich the research of the geographical distribution of ESs interaction in dryland ecosystems to provide practical ecosystem management under local conditions.

Contamination and cancer risk assessment of polycyclic aromatic hydrocarbons (PAHs) in urban dust from different land-uses in the most populated city of Iran.

Due to population growth and the considerable increase in usage of the resources, Human environment quality has been highly threatened by pollutants in recent decades. Polycyclic aromatic hydrocarbons (PAHs) are widespread, persistent organic pollutants which are of great concern due to their carcinogenicity. The present study is the first investigation that assesses contamination, sources and cancer risk of 16 priority PAHs proposed by US EPA in urban dust samples (n = 80) taken in different land-uses of Tehran metropolis, the capital of Iran. Gas chromatography-mass spectrometry (GC-MS) is used to measure PAHs concentrations. The results showed that the average concentration of the total 16 PAHs and the average Benzo[a]pyrene total potency equivalency were 566 µg kg-1 dry weight and 36.4 µg kg-1, respectively. In the commercial and residential land-uses high molecular weight (HMW) PAHs were dominated, whereas in green lands, light molecular weight (LMW) PAHs showed maximum contribution. The highest concentration of total PAHs were observed in the commercial areas due to limited air circulation and heavy traffic loads. Incremental Lifetime Cancer Risk (ILCR) model was applied to evaluate the cancer risk of exposure to PAHs contaminated dust. Based on the results, Tehran's residents (children and adults) in various land-uses except for green lands, are in high potential cancer risk of PAHs via ingestion and dermal contact exposure routs. Ace, Chr, Pyr, and BghiP which are indicators of traffic emissions, were found to be predominant PAH contributors in urban dust of commercial areas. Also, Ace, Fl, Phe, and BghiP which are derived from fossil fuel combustion, were mainly observed in the industrial land-use. Based on the results of factor analysis and diagnostic ratios, Diesel/gasoline engine vehicular emissions and combustion were found to be the main sources of PAHs in urban dust of Tehran.

Removal of mercury from contaminated saline wasters using dithiocarbamate functionalized-magnetic nanocomposite.

In this study, an efficient adsorbent was proposed for the removal of mercury from saline water contaminated with mercury ions. Fe3O4 nanoparticles were modified using tetraethylenepentamine and carbon disulfide to incorporate dithiocarbamate functional group on the surface of the adsorbent. CHNS analysis confirmed successful modification of magnetic nanoparticles. The XRD pattern of adsorbent indicated a proper match with the standard XRD pattern of cubic Fe3O4. The saturation magnetization of final adsorbent was 27 emu g-1. The morphology of bare and silica-coated Fe3O4 and final product were investigated using FE-SEM analysis. For optimizing the adsorption process, response surface methodology was applied, which was resulted in a significant quadratic model. The effect of adsorbent dosage and initial concentration of Hg (II) was much more significant than that of pH. Different concentrations of dissolved solids up to 2000 mg L-1 had no adverse effect on the adsorption process due to the strong interaction between dithiocarbamate functional group of adsorbent and Hg (II). The least values of RMSE (0.0950) and χ2 (0.0009) were observed for Radke-Prausnitz, Redlich-Peterson, and UT isotherms. Maximum adsorption capacities calculated using Langmuir and UT models were 109.5 and 95.07 mg g-1, respectively. The investigation of adsorption isotherm was conducted at the pH range of 2.0-6.5. The results showed an increase in the adsorption capacity by increasing pH. Thermodynamic studies demonstrated that the nature of the adsorption process was spontaneous and endothermic. Recovery of adsorbent was successfully carried out using HCl 0.5 mol L-1. The prepared adsorbent was successfully applied for mercury removal from a real groundwater.

Study on non-linear equilibrium, kinetics and thermodynamic of deltamethrin removal in aqueous solution using modified magnetic iron oxide nanoparticles.

The purpose of modification of magnetic iron oxide nanoparticles is an eco-friendly, emerging and economical method for removing deltamethrin in the aqueous solution and wastewater effluents when compared with other adsorbent methods. Modified magnetic iron oxide nanoparticles were synthesized by co-precipitation and then coupled with 3-hydroxytyraminium chloride. The nano-sorbent was characterized by thermogravimetric analysis, elemental analysis, transmission electron microscopy, scanning electron microscope, Fourier transform infrared spectroscopy, zero point charge and surface area determination. Batch studies were conducted and adsorption equilibrium, kinetic and thermodynamic non-linear models were carried out. The resulting equilibrium data were tested with Langmuir and Freundlich non-linear isotherm models, and the results showed that the Langmuir isotherm fitted the data well. Kinetic studies were done with different initial deltamethrin concentrations, adsorbent dosage and temperature, and the data were assimilated with pseudo-first order, pseudo-second order and intra-particle diffusion kinetic equations, and it was found that the studied nano-sorbent processes followed the pseudo-second order kinetic equation. Thermodynamic analysis was also carried out to estimate the changes in free energy (ΔG0), enthalpy (ΔH0), and entropy (ΔS0). The thermodynamic parameters revealed that the adsorption of deltamethrin into the nano-sorbent was spontaneous, feasible and showed an endothermic process.

Developing an environmental water quality monitoring program for Haraz River in Northern Iran.

Water quality management plans are an indispensable strategy for conservation and utilization of water resources in a sustainable manner. One common industrial use of water is aquaculture. The present study is an attempt to use statistical analyses in order to prepare an environmental water quality monitoring program for Haraz River, in Northern Iran. For this purpose, the analysis of a total number of 18 physicochemical parameters was performed at 15 stations during a 1-year sampling period. According to the results of the multivariate statistical methods, the optimal monitoring would be possible by only 3 stations and 12 parameters, including NH3, EC, BOD, TSS, DO, PO4, NO3, TDS, temperature, turbidity, coliform, and discharge. In other words, newly designed network, with a total number of 36 measurements (3 stations × 12 parameters = 36 parameters), could achieve exactly the same performance as the former network, designed based on 234 measurements (13 stations × 18 parameters = 234 parameters). Based on the results of cluster, principal component, and factor analyses, the stations were divided into three groups of high pollution (HP), medium pollution (MP), and low pollution (LP). By clustering the stations, it would be possible to track the water quality of Haraz River, only by one station at each cluster, which facilitates rapid assessment of the water quality in the river basin. Emphasizing on three main axes of monitoring program, including measurement parameters, sampling frequency, and spatial pattern of sampling points, the water quality monitoring program was optimized for the river basin based on natural conditions of the study area, monitoring objectives, and required financial resources (a total annual cost of about US $2625, excluding the overhead costs).

Stimulation of the hydrolytic stage for biogas production from cattle manure in an electrochemical bioreactor.

Electrical current in the hydrolytic phase of the biogas process might affect biogas yield. In this study, four 1,150 mL single membrane-less chamber electrochemical bioreactors, containing two parallel titanium plates were connected to the electrical source with voltages of 0, -0.5, -1 and -1.5 V, respectively. Reactor 1 with 0 V was considered as a control reactor. The trend of biogas production was precisely checked against pH, oxidation reduction potential and electrical power at a temperature of 37 ± 0.5°C amid cattle manure as substrate for 120 days. Biogas production increased by voltage applied to Reactors 2 and 3 when compared with the control reactor. In addition, the electricity in Reactors 2 and 3 caused more biogas production than Reactor 4. Acetogenic phase occurred more quickly in Reactor 3 than in the other reactors. The obtained results from Reactor 4 were indicative of acidogenic domination and its continuous behavior under electrical stimulation. The results of the present investigation clearly revealed that phasic electrical current could enhance the efficiency of biogas production.

A novel pollution index based on the bioavailability of elements: a study on Anzali wetland bed sediments.

In this research, we study on the distribution of several elements in bed sediments of Anzali wetland. Anzali, one of the most important international wetlands, is located on the southern coast of the Caspian Sea in Iran. This wetland receives discharges of domestic, agricultural, and industrial wastewater, which affect the distribution of elements. Our contribution in this study is threefold. First, we measured the total concentration of metals as well as their chemical partitioning and bioavailability in the sediments. Second, we calculated anthropogenic portions of metals in the sediment of this area. The results reveal anthropogenic portion of metals as Mo > Mn > Cd > As > Zn > Hg > Co > Sn > Cu > V > Ag > Ni > Pb > Fe > Cr > Al, respectively. We evaluated the intensity of pollution by using an enrichment factor, the geo-accumulation index and the pollution index. All these indices do not take into consideration the bioavailability of the elements. As our third and most important contribution, we introduced a new formula that takes into account the bioavailability of different elements. In comparison with aforementioned pollution indices, our newly introduced pollution index has a higher Pearson correlation with anthropogenic portion of metals. This high-correlation coefficient shows that our proposed pollution index is an effective indicator for determining the level of pollution, while other indices preserve their own merits.

Mechanisms and controlling factors of heavy metals removal by electroflocculation in estuarine environments.

Estuaries play a crucial role in preventing the influx of metals from rivers into seas, thereby offering potential insights for the water purification industry. This study seeks to identify the key parameters (including pH, electricity conductivity (EC), and Eh) influencing the removal efficiency of Mn, Zn, Cu, Co, and Ni during natural and electro-flocculation processes in the Siahroud River estuary. The experiments were conducted in three stages, each representing varying salinity levels and voltage conditions, to determine the most effective parameters for metal removal. The findings revealed that heavy metal flocculation rates were highest at lower salinities (0.5 to 1.5 PSU), with no significant improvement in contaminant removal observed with increasing voltage. Electro-flocculation efficiency was found to be more dependent on Eh. Overall, the flocculation processes reduced the annual total dissolved metal content from 14.84 to 6.46 tons, underscoring the potential of this method in water quality management.

Speciation characteristics, ecological risk assessment, and source apportionment of heavy metals in the surface sediments of the Gomishan wetland.

Metal contamination is one of the worldwide environmental issues. The main aim of this study was to evaluate the concentration, probable environmental risk, and source of investigated elements (Al, As, Co, Cr, Cu, Fe, Mn, Ni, and Zn) in sediments and water of the Gomishan wetland. Sediment contamination indices revealed sediments were solely polluted by As. The potential ecological risk index (RI), toxic risk index (TRI), and chemical speciation assessments indicated no major ecological hazards for investigated metals. Correlation analysis and principal component analysis (PCA) indicated that all studied metals in the Gomishan wetland sediments derived from natural sources. HPI, and HEI indices showed that the water quality in terms of hazardous components was inappropriate for aquatic life.

Biodiesel production through transesterification of waste Pistacia- Terebinthus Oil by pharmaceutical waste as a heterogeneous catalyst: A sustainable solution for reducing external costs.

A catalyst from the pharmaceutical waste of calcium and magnesium tablets was synthesized for biodiesel production from waste Pistacia-Terebinthus (PT) oil with the aim of creating added value and presenting a new approach for the management of such wastes. For this purpose, magnesium and calcium tablet wastes with a mass ratio of 70:30 (wt%) were calcined. The catalyst was investigated by several methods, such as thermal gravimetric analysis, X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, Brunauer-Emmett-Teller analysis, X-ray photoelectron spectroscopy, and CHNS/O elemental analysis. The high specific surface area of the catalyst confirms that the utilized synthesis method resulted in the formation of a high number of active sites in its structure, which allows it to function as a suitable catalyst for this reaction. Furthermore, the impact of effective parameters on the treansestrification reaction was optimized and investigated by designing the experiments and applying the RSM method. The maximum mass yield of 96 % was obtained in optimal conditions (temperature of 70 °C, catalyst loading of 4.498 wt%, methanol:oil ratio of 1.968 (vol:vol), and reaction time of 120 min). The reusability of the catalyst was investigated in four successive cycles. The mass yield of the last test declined from 96 % to 71.4 %. Gas chromatography-mass spectrometry analysis of the produced biofuel revealed that it comprises 91.37 % methyl ester compounds (64.28 % 12-Octadecenoic Acid, Methyl Ester). To evaluate the external costs of biofuel (B100) and compare it with diesel, combustion simulation was done with Diesel-RK software, which showed that its external costs were 0.05388 (€/Lit fuel) less than those of diesel.

Investigating heavy metal pollution in Anzali coastal wetland sediments: A statistical approach to source identification.

In this study, the pollution and bioavailability of heavy metals in the sediments of Anzali Wetland were measured by analyzing data from sequential chemical extraction of sediments, risk assessment code (RAC), and sediment pollution indices. The average RAC results indicated that the risk from Zn, Cr, Cu, and Hg was low, while the risk from Pb, Ni, As, and Cd was moderate. To identify the sources of heavy metal pollution in the sediments of Anzali Wetland, multivariate statistical techniques such as Pearson correlation analysis, cluster analysis (CA), and principal component analysis (PCA) were employed. The results of the statistical analyses at a high significance level revealed that Zn, Cr, Cu, Pb, Ni, and As were attributed to natural sources. Additionally, the statistical analyses demonstrated that the concentrations of Cd and Hg in the sediments of Anzali Wetland were influenced by non-oil organic sources and atmospheric deposition, respectively.

Development and application of novel risk indices for assessing heavy metal pollution in aquatic sediments.

This study presents the development of a new sediment individual risk index (SIRI) and sediment complex risk index (SCRI) to assess heavy metal pollution in Anzali Wetland sediments. SIRI incorporates total metal concentrations, bioavailability, and sediment quality guidelines. SCRI, derived through principal component analysis (PCA), integrates SIRI for a comprehensive risk assessment. The newly developed indices were systematically classified. Results showed varying risk levels with SIRI values of Zn, Cr, Cu, Pb, Ni, As, Cd, and Hg as 0.82, 1.32, 0.98, 0.71, 1.41, 1.37, 0.79, and 0.79, respectively. Pb, Cd, and Hg posed very low risk, Cu and Zn posed low risk, and Cr, Ni, and As posed moderate risk. SCRI yielded an index value of 1.02, indicating a moderate level of risk for the studied stations. Pearson correlation analysis validated SCRI with a highly significant and strong correlation coefficient (0.923) with metal bioavailability, serving as a risk indicator.

The effects of socioeconomic parameters on household solid-waste generation and composition in developing countries (a case study: Ahvaz, Iran).

Environment problems associated with the generation of waste are part of societal changes where households play an important role. These societal changes influence the size, structure and characteristic of given households. For the effective planning of solid-waste handling infrastructure, it is essential to know the quantity of waste generation and its composition. This paper presents the findings of a study carried out in an urban municipal area in Iran to determine the household solid-waste generation rate and waste composition based on field surveys and to determine the related socioeconomic parameters. The dependent variables were waste generation and composition, and independent variables were family size, family employment, age, number of room and education. Over 400 sample households were selected for the study using a stratified random sampling methodology and from five different socioeconomic groups. Waste collected from all groups of households were segregated and weighted. Waste generation rate was 5.4 kg/household/day. Household solid waste comprised of ten categories of wastes and with the largest component (76.9%). The generation and composition of household solid waste were correlated with family size, education level and households income. This paper adequately suggests new insights concerning the role of socioeconomic parameters in affecting the generation of household waste.

Bioaccumulation of trace elements in different tissues of three commonly available fish species regarding their gender, gonadosomatic index, and condition factor in a wetland ecosystem.

In the present research, accumulation of Cd, Co, Cr, Cu, Mn, Ni, Pb, V, and Zn were determined in sediments and fillet, liver, kidney, gonads, and gills of three commonly fish species in the largest wetland ecosystem that is located in southwest of Iran; Shadegan wetland. Shadegan is one of the most important wetland that posses various fauna and flora but suffers inputs from agricultural and industrial activities. So, sediment samples and fish species including Barbus grypus, Barbus sharpeyi, and Cyprinus carpio were collected during winter 2009. Results showed that mean concentrations of trace elements (except Ni and Co) were high in liver and gills of B. grypus. Also trace elements had the most accumulation in liver of B. sharpeyi except for Cd (0.26 mg kg(- 1) d.w.) and Mn (13.45 mg kg(- 1) d.w.) that were high in gills. Beside, kidney is determined as target tissue for Ni and V in B. grypus and for Pb in C. carpio, due to their high concentration. Zn levels in all tissues of C. carpio showed the highest concentrations in comparison to other fish species. Generally, accumulations of most of the studied elements in B. grypus and B. sharpeyi were higher in females than in males, while in fillet and gonads of C. carpio, this trend was inverted. Bioaccumulation factors (BAFs) were determined for different tissues of fish species with respect to elemental concentrations in sediment. BAFs results indicated that Zn, Pb, and Cu have higher BAF than other elements. Also this investigation demonstrated that trace elements have different affinities with condition factor of studied fish species. Gonadosomatic index (GSI) and Pb showed positive correlation together in both B. sharpeyi and B. grypus, respectively, in females and males. Moreover, females of C. carpio showed significantly positive relation of GSI and all studied elements.

Assessment and investigation on the fate of organochlorine pesticides in water and sediments of international Amir-kalaye wetland in north of Iran.

A total of 48 water samples and 24 sediment samples were collected at four sampling stations along the wetland during four seasons from 2009 to 2010 and analyzed by gas chromatograph-electron capture detector (GC-ECD). In water the total concentration of OCPs was 0.33, 0.01, 0.1 and 0.07 mg/L in summer, autumn, winter and spring, respectively. The most frequent OCP compounds detected were endrin and chlordane (0.08 and 0.07 mg/L), heaxachlorobenzene and chlordane (0.06, 0.02 mg/L), and chlordane (0.07 mg/L) in summer, winter and spring, respectively. The maximum concentration of ΣOCPs was found in samples collected from station 1 in summer (0.26 mg/L). In sediments the total concentrations of OCPs were 15.84 and 2.62 mg/g-dry weight (dw) in summer and winter, respectively. Chlordane was the most frequently found OCP compound, followed by lindane, 9.92 and 2.47 mg/g-dry weight (dw), respectively, in summer. While, lindane (2.52 mg/g-dw) and endosulfan I (0.1 mg/g-dw) were the highest OCP compounds detected in winter. The results obtained in this study show that there still exist a variety of organochlorine pesticide residues in the water and sediments from the Amir-kalaye wetland in Iran.

Determining the appropriate mixing ratio in a multi-substrate anaerobic digestion of organic solid wastes employing Taguchi method.

The current research proposed a method for optimally combining feed input ratios in order to improve the quantity and quality of daily biogas production through optimizing the variable response level in the Taguchi method. The anaerobic digestion (AD) process of an existing plant in Iran was simulated through a set of two-stage pilot reactors under mesophilic temperature conditions in order to achieve optimal operational performance. Three common substrates (organic fraction of municipal solid wastes, fruit and vegetable wastes, and horse manure) along with two recirculated materials; the post-digestion sludge and the secondary digester slurry, were investigated in 16 experimental runs based on four different pre-surface hypotheses. Comparison of the results of daily biogas energy (J/d/g-VS) in Run#9, to which the actual yield of hydrogen sulfide was minimal in parallel to a methane yield above 100 mL/g-VS, with the result of the optimal run with the ratios provided by the model, showed that the daily biogas energy was improved by 50% comparing to the control Run that had similar conditions to which was being applied in the full-scale existing AD plant.