The baseline diameter of the inferior vena cava measured by sonography increases with age in normovolemic children.

OBJECTIVES: To evaluate normative sonographic measurements of the inferior vena cava (IVC) diameter in healthy pediatric patients. METHODS: We performed a prospective observational study of a convenience sample of healthy patients between the ages of 0 and 22 years presenting to a pediatric emergency department. Exclusion criteria included abnormal vital signs, pregnancy, or illnesses thought to influence volume status. During quiet respiration, the maximum and minimum IVC diameters were measured in the sagittal plane distal to the hepatic vein-IVC junction. As second measurements, the maximum diameters of the IVC and aorta were measured in the transverse plane distal to the insertion of the left renal vein into the IVC. RESULTS: From February 2013 through April 2014, 63 children (51% female; mean age, 11 years) were enrolled. There were 20 children in each age group of 2 to 7, 7 to 12, and 12 to 22 years. The correlations between IVC and aortic diameters as a function of age were calculated using the Spearman rank correlation coefficient. The correlation coefficients were all statistically significant (P < .001): sagittal maximum IVC diameter (0.81), sagittal minimum IVC diameter (0.79), transverse maximum IVC diameter (0.79), and transverse maximum aortic diameter (0.81). CONCLUSIONS: This pilot study of sonographic measurements of the IVC diameter in normovolemic children suggests a statistically significant positive correlation between age and IVC diameter. Future studies should focus on multicenter enrollment, children in the youngest age group, and the development of normative growth curves for the IVC by age, sex, and body mass index.

Quantitative analysis of the microvasculature growing in the fibrin interface between a skin graft and the recipient site.

Current tissue engineering techniques have failed to provide an established microvasculature in skin substitutes, a requisite for the maintenance of graft viability and rapid revascularization subsequent to graft transplantation in vivo. To improve outcomes for both conventional skin grafts and skin substitutes, the existing knowledge gap concerning the spatio-temporal mechanisms of skin graft revascularization must be abrogated. The current knowledge gap is due, at least in part, to a lack of appropriate diagnostic methods to quantify skin graft revascularization. To enhance the understanding of skin graft revascularization, we quantitatively evaluated revascularization of autologous skin grafts in a rat model by quantifying 2- and 3-dimensional vascular metrics in the fibrin interface 3, 7, and 10 days after transplantation. In this study, the fibrin interface appeared to be completely replaced with fibrovascular tissue by postoperative day 10. Although the mean vessel diameter was about 10 mum for the time points sampled, the mean vessel number, area, and volume fraction increased about 2.5-fold from postoperative day 3 to 7 and then decreased about 1.27-fold at postoperative day 10. There was no significant difference between 2- and 3-dimensional vascular metrics based on Bland-Altman analysis. In conclusion, these data establish a standard for metrics of vessels growing in the fibrin interface of a rat autologous skin graft and its donor site and suggests that once the blood supply has been restored to a viable transplant, the number, area, and volume fractions of vessels decrease to levels found at postoperative day 3.