Fusiform vateritic inclusions observed in European eel (Anguilla anguilla L.) sagittae.

Microscopic inclusions have been observed in 7 out of 106 European eel (Anguilla anguilla L.) sagittae using polarizing microscope and scanning electron microscope meanwhile the annual increments were studied to characterize the age structure of the population living in Lake Balaton. The presence of vaterite, a rare calcium carbonate polymorph was observed in these inclusions using Raman spectroscopy. Vateritic sagittae in wild fish are usually considered as symptom of physiological stress. The observed fusiform inclusions represent a new morphological type of vaterite inclusions in eel otolith. Two alternatives are hypothesized to explain their formation: 1) metabolic disorder, such as erroneous protein synthesis; 2) introduction of an alien protein into the eel's inner ear. The origin and physiological significance of this new morphological type of vateritic inclusions is still an open question. Same as whether it can be found in other species or specific only to eel otoliths.

DOCU-CLIM: A global documentary climate dataset for climate reconstructions.

Documentary climate data describe evidence of past climate arising from predominantly written historical documents such as diaries, chronicles, newspapers, or logbooks. Over the past decades, historians and climatologists have generated numerous document-based time series of local and regional climates. However, a global dataset of documentary climate time series has never been compiled, and documentary data are rarely used in large-scale climate reconstructions. Here, we present the first global multi-variable collection of documentary climate records. The dataset DOCU-CLIM comprises 621 time series (both published and hitherto unpublished) providing information on historical variations in temperature, precipitation, and wind regime. The series are evaluated by formulating proxy forward models (i.e., predicting the documentary observations from climate fields) in an overlapping period. Results show strong correlations, particularly for the temperature-sensitive series. Correlations are somewhat lower for precipitation-sensitive series. Overall, we ascribe considerable potential to documentary records as climate data, especially in regions and seasons not well represented by early instrumental data and palaeoclimate proxies.

Cellulose in Foliage and Changes during Seasonal Leaf Development of Broadleaf and Conifer Species.

Stable isotope approaches are widely applied in plant science and many improvements made in the field focus on the analysis of specific components of plant tissues. Although technical developments have been very beneficial, sample collection and preparation are still very time and labor-consuming. The main objective of this study was to create a qualitative dataset of alpha-cellulose content of leaf tissues of arboreal species. We extracted alpha-cellulose from twelve species: Abies alba Mill., Acer pseudoplatanus L., Fagus sylvatica L., Larix decidua Mill., Picea abies (L.) Karst., Pinus sylvestris L., Quercus cerris L., Quercus petrea (Matt.) Liebl., Quercus pubescens Wild., Quercus robur L., Tilia platyphyllos Scop. and Ulmus glabra Huds. While these species show an increase in cellulose yield from bud break to full leaf development, the rates of increase in cellulose content and the duration of the juvenile phase vary greatly. Moreover, the veins display significantly higher alpha-cellulose content (4 to 11%) compared to blade tissues, which reflects their different structural and biochemical functions. A guide for the mass of sample material required to yield sufficient alpha-cellulose for a standard stable isotope analysis is presented. The additional benefits of the assessment of the mass of required sample material are reduced sample preparation time and its usefulness in preparing samples of limited availability (e.g., herbarium material, fossil samples).

Geostatistical evaluation of the design of the precipitation stable isotope monitoring network for Slovenia and Hungary.

A detailed knowledge of the stable isotope signature of precipitation is the basis of investigations in a variety of scientific fields and applications. To obtain robust and reliable results, the representativity of the currently operating (at least, as of 2018) precipitation stable isotope monitoring stations across Slovenia (n = 8) and Hungary (n = 9) was evaluated on the basis of amount-weighted annual averages with the aim of revealing any redundantly (i.e. over-) represented or un(der)represented areas. In the case of the latter, optimal locations for additional sites were suggested in Slovenia and Hungary. The networks of both countries are design-based systems that need to be fine-tuned for long-term optimized operation. The evaluation of the monitoring network was performed taking into consideration the stations operating in Slovenia and Hungary, as well as closely situated ones operating in neighboring countries. The evaluation was carried out in nine different combinations, using spatial simulated annealing, with regression kriging variance as a quality measure. The results showed that (i) there are over- and un(der)represented areas in the network, an issue requiring remedial action, (ii) the mutual information exchange of the precipitation stable isotope monitoring networks of Slovenia and Hungary increases the precision of precipitation δ18O estimation by ~0.3‰ in a 15-30 km wide zone near the borders, and (iii) by an even greater degree in the neighboring countries' stations. The current research may be termed pioneering in the matter of the detailed geostatistical assessment of spatial representativity of a precipitation stable isotope monitoring network, and as such, can serve as an example for future studies aiming for the spatial optimization of other regional precipitation stable isotope monitoring networks.

Monthly data of stable isotopic composition (δ18O, δ2H) and tritium activity in precipitation from 2004 to 2017 in the Mecsek Hills, Hungary.

The stable isotopic composition (δ18O, δ²H) and tritium activity of monthly aggregated precipitation samples were collected between April 2004 and December 2017 at six sites representing the first published precipitation isotope dataset from the Mecsek Hills (Hungary). The dataset includes 697 stable isotopic and 653 tritium activity concentration data of monthly precipitation samples collected across the Mecsek Hills. At the beginning of the monitoring period, the isotopic composition values suggest an insufficient protection against evaporation and this issue has occasionally reappeared later only in limited periods. These data are presented in brackets in the Supplementary Table and should be disregarded from further analysis until additional verification. This dataset provides isotope hydrological benchmark in comparison with other local and regional datasets of stable isotopes and tritium activities in surface water and groundwater not only in the Mecsek Hills but also in the surroundings. It can support water resource management, and paleoclimatological research. Isotope hydrological evaluation and further discussion on the seasonal trends in the precipitation isotopic characteristics are in progress and the tritium data were used in the derivation of a gridded database (1 × 1 km) of amount-weighted annual mean precipitation tritium activity for the Adriatic-Pannonian Region (AP3H_v1, [1]).

Fallout isotope chronology of the near-surface sediment record of Lake Bolatau.

Fallout isotope (210Pbex,137Cs and 241Am) based dating has been carried out on the near-surface sediment core collected from Lake Bolatau-Feredeu (Bukovina, Romania). The motivation was to improve the chronology of this recent section in connection with significant fluctuations observed in sediment accumulation rates, particle size distribution and primordial radioisotope (i.e. 40K and 232Th) composition. Previously only an extrapolation of a broad-range OxCal age-depth model, which was based on 8 AMS radiocarbon dates from the deeper part of a parallel sediment sequence and tentatively validated for the upper part using the double peaks of the 137Cs activity concentration distribution, was available for the studied section (1-24 cm). Parallel to the previous 137Cs measurement, 210Pb and 226Ra (for a more detailed, 210Pbex-based chronology), 241Am (for an additional time-marker), as well as 40K and 232Th concentrations have also been determined by gamma-spectrometry. In case of the 210Pbex-based chronology, due to a large deviation from a pure exponential distribution, the Constant Flux (CF) model has been used for the calculation of sediment ages and accumulation rates. Although the broad-range OxCal and the CF model were broadly similar down to 22 cm, the 210Pbex-based ages are clearly superior in terms of uncertainty in the uppermost 12 cm, while the broad-range model has smaller uncertainty below 20 cm (>150 years). The CF model gave an average mass accumulation rate of (0.08 ± 0.03) g cm-2 yr-1 for sections 0-11 cm, and (0.03 ± 0.01) g cm-2 yr-1 for sections 12-22 cm, respectively. Significant changes have been observed in the depth distribution of both the particle size distribution and the elemental/isotopic composition of the sediment record, most likely related to the variation observable in the intensity and volume of precipitation in the catchment. The obtained high-resolution records of Lake Bolatau, including multiple radioisotopes, can serve as a regional benchmark for similar studies.

Stable isotope signatures of seasonal precipitation on the Pacific coast of central Panama.

As calculated from data archived in the IAEA-WMO Global Network of Isotopes in Precipitation programme, the amount-weighted local meteoric water line for the Pacific coast of central Panama is: δ(2)H = 7.63(±0.08) × Î´(18)O + 6.51(±0.49). Amount-weighted mean isotopic values were regressed against the sea surface temperature (SST) fields of the adjacent tropical oceans. A negative correlation of precipitation isotope composition with Caribbean SSTs is observed only for the early wet season (May-June), whilst the mid-summer dry period is characterized by positive correlation with eastern Pacific SSTs, similar to the late wet season (October-November). The negative response of May-June rainfall isotopic composition to Caribbean SSTs is explained by a SST-mediated change in stratiform rain fraction from organized convective systems proximal to the Inter Tropical Convergence Zone (ITCZ). The positive correlation for the rest of the wet season, when the organized convective zone of ITCZ and its attached stratiform belt are distant from the Pacific coast of Panama, is interpreted as simple evaporative temperature effect on isotopic fractionation.

Qualitative Distinction of Autotrophic and Heterotrophic Processes at the Leaf Level by Means of Triple Stable Isotope (C-O-H) Patterns.

Foliar samples were harvested from two oaks, a beech, and a yew at the same site in order to trace the development of the leaves over an entire vegetation season. Cellulose yield and stable isotopic compositions (δ(13)C, δ(18)O, and δD) were analyzed on leaf cellulose. All parameters unequivocally define a juvenile and a mature period in the foliar expansion of each species. The accompanying shifts of the δ(13)C-values are in agreement with the transition from remobilized carbohydrates (juvenile period), to current photosynthates (mature phase). While the opponent seasonal trends of δ(18)O of blade and vein cellulose are in perfect agreement with the state-of-art mechanistic understanding, the lack of this discrepancy for δD, documented for the first time, is unexpected. For example, the offset range of 18 permil (oak veins) to 57 permil (oak blades) in δD may represent a process driven shift from autotrophic to heterotrophic processes. The shared pattern between blade and vein found for both oak and beech suggests an overwhelming metabolic isotope effect on δD that might be accompanied by proton transfer linked to the Calvin-cycle. These results provide strong evidence that hydrogen and oxygen are under different biochemical controls even at the leaf level.