RESUMO
After lindane was administered to rats in a single oral dose, the time profile of the degree of conjugation of the main phenolic metabolites was evaluated. In all urine samples 2,3-dichlorophenol, 2,4,5- and 2,4,6-trichlorophenol, and 2,3,5,6-tetrachlorophenol were constantly present. 2,3,5,6-Tetrachlorophenol and then 2,4,6-trichlorophenol were the prevalent metabolites in all cases. The degree of conjugation did not correlate with the dissociation constant of individual chlorinated phenols. Phenol conjugation declined with time after administration.
Assuntos
Clorofenóis/urina , Hexaclorocicloexano/metabolismo , Administração Oral , Animais , Clorofenóis/metabolismo , Cromatografia Gasosa/métodos , Eletroquímica , Hexaclorocicloexano/administração & dosagem , Masculino , Ratos , Ratos Endogâmicos , Fatores de TempoRESUMO
A toxicological method of sensitive and specific confirmation of methamphetamine and other primary and secondary amines in biological samples after extractive perfluorobenzoylation is described. The method is based on the principle of gas chromatography with parallel specific nitrogen detection and electron capture detection. The other alternative is the gas chromatography combined with mass spectrometry in chemical ionization mode. The method described allows the detection of methamphetamine and amphetamine in urine in concentrations below 10 ng/ml.