Non-invasive Microneedle Application Increases Ceramide and Natural Moisturizing Factors in a Reconstructed Human Skin Model.

Recently, microneedling as a cosmetic product has attracted attention as one way to improve skin barrier function and moisturizing function to reduce wrinkle formation. However, some cases of erythema and edema have been reported as side effects. In order to develop safer microneedle cosmetics, we investigated whether microneedles can improve skin barrier function and moisturizing function even when applied in a non-invasive manner that does not penetrate the stratum corneum. We established the condition of non-penetrating microneedle application on reconstructed human full-thickness skin models and examined the effect on the skin models when microneedles were applied under this condition. Microneedle application increased the gene expression of serine palmitoyltransferase long chain base subunit (SPTLC) 3, filaggrin, and transglutaminase 1. The amount of ceramide produced by SPTLC was also increased by microneedle application. Gene expression of filaggrin-degrading enzymes and the amount of free amino acids, a product of filaggrin degradation, were also increased by microneedling. These results suggest that non-invasive microneedle application can improve skin barrier function and moisturizing function by increasing the amount of ceramide and natural moisturizing factors.

Biomechanical analysis for total mesialization of the maxillary dentition: A finite element study.

INTRODUCTION: The purpose of this study was to analyze and clarify tooth movement during mesialization of the whole maxillary dentition with various force angulations (FAs). METHODS: A finite element method was used to simulate the long-term orthodontic movement of the maxillary dentition by accumulating the initial displacement of teeth produced by elastic deformation of the periodontal ligament. A mesial force of 3 N was applied to the maxillary second molar at 5 different FAs (-30°, -15°, 0°, 15°, and 30°) to the occlusal plane. RESULTS: At an FA of 28°, the line of action of the force passed through the center of resistance of the maxillary whole dentition. With all FAs, the central incisors and molars tipped labially and mesially, respectively. The tipping angles gradually decreased as the FAs shifted from -30° to 30°. The molars tipped lingually with FAs of -30° and -15°, whereas they tipped buccally with FAs of 0°, 15°, and 30°. The molars tended to rotate mesiolingually more as the angle of force increased toward an FA of 30°. The occlusal plane rotated counterclockwise with FAs of -30°, -15°, and 0°, whereas it rotated clockwise with FAs of 15° and 30°. With an FA of 30°, buccal tipping and mesiolingual rotation of the molars, and the change in the occlusal plane angle decreased when the transpalatal arch (TPA) was fixed to the first molars and decreased, even more when the TPA was fixed to the second molars rather than the first molars, when a thicker TPA was used, and when the TPA was fixed to both molars rather than a single molar. CONCLUSIONS: There was a correlation between tooth movement during mesialization of the whole maxillary dentition and the angle at which the force was applied.

Biomechanical analysis for total distalization of the maxillary dentition: A finite element study.

INTRODUCTION: This study aimed to identify the tooth movement patterns relative to various force angulations (FAs) when distalizing the total maxillary dentition. METHODS: Long-term orthodontic movement of the maxillary dentition was simulated by accumulating the initial displacement of teeth produced by elastic deflection of the periodontal ligament using a finite element analysis. Distalization forces of 3 N were applied to the archwire between the maxillary canine and first premolar at 5 different FAs (-30°, -15°, 0°, 15°, and 30°) to the occlusal plane. RESULTS: Maxillary incisors and molars showed lingual and distal tipping at all FAs, respectively. At a force angulation of 30°, almost bodily distalization of the total maxillary dentition occurred, but incisors showed considerable lingual tipping because of the effect of clearance gap (0.003-in, 0.022 × 0.025-in bracket slot, 0.019 × 0.025-in archwire) and elastic deflection of the archwire. Medial displacement of the maxillary anterior teeth occurred because of lingual tipping during distalization. The occlusal plane rotated clockwise at all FAs because of extrusion of the maxillary incisors and intrusion of the maxillary second molars, and the amounts decreased as FA increased. CONCLUSIONS: Tooth movement patterns during distalization of the total maxillary dentition were recognized. With an understanding of the mechanics, a proper treatment plan can be established.

Biomechanical analysis for total mesialization of the mandibular dentition: A finite element study.

OBJECTIVES: To clarify the mechanics of tooth movement in mesialization of the whole mandibular dentition when changing the force angulation. SETTING: A finite element method was used to simulate long-term movements of the whole mandibular dentition. MATERIAL AND METHODS: Tooth movement was simulated by accumulating the initial displacement, which was produced by elastic deformation of the periodontal ligament. Mesial forces of 3 N were applied to the second molar bracket at -30°, -15°, 0°, 15° and 30° to the occlusal plane. RESULTS: The whole dentition and occlusal plane were rotated depending on the direction of the force with respect to the centre of resistance (CR). At a force angulation of -30°, the line of action of the force passed near the CR, and the whole dentition translated without rotation of the occlusal plane. The second molar tipped buccally due to a clearance gap between the archwire and bracket slot. When increasing a force angulation from -30°, the line of action of the force passed above the CR, and thereby, the occlusal plane rotated clockwise. This rotation of the whole dentition induced tipping of the individual teeth. Buccal tipping of the molar due to an elastic deformation of the archwire was prevented by using a lingually pre-bent archwire. CONCLUSIONS: Careful selection of force angulation and biomechanics is essential to obtain proper tooth movement in total mesialization of the mandibular dentition.

Biomechanical analysis for total distalization of the mandibular dentition: A finite element study.

INTRODUCTION: The aim of this finite element study was to analyze and clarify the mechanics of tooth movement patterns for total distalization of the mandibular dentition based on force angulation. METHODS: Long-term orthodontic movement of the mandibular dentition was simulated by accumulating the initial displacement of teeth produced by elastic deformation of the periodontal ligament. RESULTS: Displacement of each tooth was caused by movement of the whole dentition, elastic deflection of the archwire, and clearance gap between the archwire and bracket slot. The whole dentition was rotated clockwise or counterclockwise when the line of action of the force passed below or above the center of resistance. Elastic deflection of the archwire induced a lingual tipping of the anterior teeth. It became larger when increasing the magnitude of angulation. The archwire could be rotated within the clearance gap between the archwire and the bracket slot, and thereby the teeth tipped. CONCLUSIONS: Mechanics of total mandibular distalization was clarified. Selective use of force angulation with a careful biomechanical understanding can achieve proper distalization of the whole mandibular dentition.

A finite element simulation of initial movement, orthodontic movement, and the centre of resistance of the maxillary teeth connected with an archwire.

The purpose of this article is to simulate long-term movement of maxillary teeth connected with an archwire and to clarify the difference between the initial tooth movement and the long-term orthodontic movement. Initial tooth movement was calculated based on the elastic deformation of the periodontal ligament. Orthodontic tooth movement was simulated based on the bone remodeling law of the alveolar bone, while consequentially updating the force system. In the initial tooth movement, all teeth tipped individually due to an elastic deflection of the archwire. In the long-term movement, the maxillary teeth moved as one united body, as if the archwire were a rigid material. Difference of both movement patterns was due to the change in force system during tooth movement. The long-term movement could not be predicted from the initial tooth movement. Movement pattern and location of the centre of resistance in the long-term movement were almost the same as those in the initial tooth movement as calculated by assuming the archwire to be a rigid material.

Distal movement of the mandibular dentition with temporary skeletal anchorage devices to correct a Class III malocclusion.

This case report describes the treatment of an 18-year-old man with a skeletal Class III pattern and a full-step Class III malocclusion. The orthodontic treatment included distal movement of the mandibular dentition with temporary skeletal anchorage devices. The total active treatment time was 30 months. His occlusion and facial appearance were significantly improved by the orthodontic treatment. Posttreatment records 2 years later showed excellent results with good occlusion and facial balance.

Finite element analysis of the effect of force directions on tooth movement in extraction space closure with miniscrew sliding mechanics.

INTRODUCTION: Miniscrews placed in bone have been used as orthodontic anchorage in extraction space closure with sliding mechanics. The movement patterns of the teeth depend on the force directions. To move the teeth in a desired pattern, the appropriate direction of force must be selected. The purpose of this article is to clarify the relationship between force directions and movement patterns. METHODS: By using the finite element method, orthodontic movements were simulated based on the remodeling law of the alveolar bone. The power arm length and the miniscrew position were varied to change the force directions. RESULTS: When the power arm was lengthened, rotation of the entire maxillary dentition decreased. The posterior teeth were effective for preventing rotation of the anterior teeth through an archwire. In cases of a high position of a miniscrew, bodily tooth movement was almost achieved. The vertical component of the force produced intrusion or extrusion of the entire dentition. CONCLUSIONS: Within the limits of the method, the mechanical simulations demonstrated the effect of force direction on movement patterns.

Esthetic orthodontic treatment with a double J retractor and temporary anchorage devices.

This clinical article reports an esthetic treatment option for managing a Class II malocclusion in an adult. The patient, a woman aged 24 years 2 months, had crowding and a convex profile. She was treated with maxillary first premolar extractions, a double J retractor, and temporary skeletal anchorage devices in the maxillary arch. Posttreatment records after 2 years showed excellent results with good occlusion and long-term stability.

Numerical simulations of canine retraction with T-loop springs based on the updated moment-to-force ratio.

The purpose of this study was to develop a new finite element method for simulating long-term tooth movements and to compare the movement process occurring in canine retraction using a T-loop spring having large bends and with that having small bends. Orthodontic tooth movement was assumed to occur in the same manner as the initial tooth movement, which was controlled by the moment-to-force (M/F) ratios acting on the tooth. The M/F ratios were calculated as the reaction forces from the spring ends. For these M/F ratios, the teeth were moved based on the initial tooth movements, which were calculated by using the bilinear elastic model of the periodontal ligament. Repeating these calculations, the teeth were moved step by step while updating the M/F ratio. In the spring with large bends, the canine at first moved bodily, followed by root distal tipping. The bodily movement was quickly achieved, but over a short distance. In the spring with small bends, the canine at first rotated and root mesial tipping occurred, subsequently the canine uprighted and the rotation decreased. After a long time elapsed, the canine moved bodily over a long distance. It was found that the long-term tooth movement produced by the T-loop springs could be simulated by the method proposed in this study. The force system acting on the teeth and the movement type remarkably changed during the long-term tooth movement. The spring with large bends could move the canine bodily faster than that with small bends.

Numeric simulations of en-masse space closure with sliding mechanics.

INTRODUCTION: En-masse sliding mechanics have been typically used for space closure. Because of friction created at the bracket-wire interface, the force system during tooth movement has not been clarified. METHODS: Long-term tooth movements in en-masse sliding mechanics were simulated with the finite element method. RESULTS: Tipping of the anterior teeth occurred immediately after application of retraction forces. The force system then changed so that the teeth moved almost bodily, and friction occurred at the bracket-wire interface. Net force transferred to the anterior teeth was approximately one fourth of the applied force. The amount of the mesial force acting on the posterior teeth was the same as that acting on the anterior teeth. Irrespective of the amount of friction, the ratio of movement distances between the posterior and anterior teeth was almost the same. By increasing the applied force or decreasing the frictional coefficient, the teeth moved rapidly, but the tipping angle of the anterior teeth increased because of the elastic deflection of the archwire. CONCLUSIONS: Finite element simulation clarified the tooth movement and the force system in en-masse sliding mechanics. Long-term tooth movement could not be predicted from the initial force system. The friction was not detrimental to the anchorage. Increasing the applied force or decreasing the friction for rapid tooth movement might result in tipping of the teeth.

Palatal en-masse retraction of segmented maxillary anterior teeth: A finite element study.

OBJECTIVE: The aim of this finite element study was to clarify the mechanics of tooth movement in palatal en-masse retraction of segmented maxillary anterior teeth by using anchor screws and lever arms. METHODS: A three-dimensional finite element method was used to simulate overall orthodontic tooth movements. The line of action of the force was varied by changing both the lever arm height and anchor screw position. RESULTS: When the line of action of the force passed through the center of resistance (CR), the anterior teeth showed translation. However, when the line of action was not perpendicular to the long axis of the anterior teeth, the anterior teeth moved bodily with an unexpected intrusion even though the force was transmitted horizontally. To move the anterior teeth bodily without intrusion and extrusion, a downward force passing through the CR was necessary. When the line of action of the force passed apical to the CR, the anterior teeth tipped counterclockwise during retraction, and when the line of action of the force passed coronal to the CR, the anterior teeth tipped clockwise during retraction. CONCLUSIONS: The movement pattern of the anterior teeth changed depending on the combination of lever arm height and anchor screw position. However, this pattern may be unpredictable in clinical settings because the movement direction is not always equal to the force direction.

Effects of transpalatal arch on molar movement produced by mesial force: a finite element simulation.

INTRODUCTION: The transpalatal arch (TPA), which splints together 2 maxillary molars, has been believed to preserve anchorage. The purpose of this study was to clarify this effect from a mechanical point of view. METHODS: The finite element method was used to simulate the movement of anchor teeth subjected to mesial forces with and without a TPA. RESULTS: In the initial movement produced by elastic deformation of the periodontal ligament, stress magnitude in the periodontal ligament was not changed by the TPA. In the orthodontic movement produced by bone remodeling, the mesial force tipped the anchor teeth irrespective of the TPA. The tipping angles of anchor teeth with and without the TPA were almost the same. The anchor teeth without the TPA were rotated in the occlusal plane and moved transversely. CONCLUSIONS: The TPA had no effect on the initial movement. In the orthodontic movement, the TPA had almost no effect, preserving anchorage for mesial movement. However, the TPA prevented rotational and transverse movements of the anchor teeth. These results are valid when the assumptions used in this calculation are satisfied.

Prediction of optimal bending angles of a running loop to achieve bodily protraction of a molar using the finite element method.

OBJECTIVE: The purpose of this study was to predict the optimal bending angles of a running loop for bodily protraction of the mandibular first molars and to clarify the mechanics of molar tipping and rotation. METHODS: A three-dimensional finite element model was developed for predicting tooth movement, and a mechanical model based on the beam theory was constructed for clarifying force systems. RESULTS: When a running loop without bends was used, the molar tipped mesially by 9.6° and rotated counterclockwise by 5.4°. These angles were almost similar to those predicted by the beam theory. When the amount of tip-back and toe-in angles were 11.5° and 9.9°, respectively, bodily movement of the molar was achieved. When the bend angles were increased to 14.2° and 18.7°, the molar tipped distally by 4.9° and rotated clockwise by 1.5°. CONCLUSIONS: Bodily movement of a mandibular first molar was achieved during protraction by controlling the tip-back and toe-in angles with the use of a running loop. The beam theory was effective for understanding the mechanics of molar tipping and rotation, as well as for predicting the optimal bending angles.

A numerical simulation of tooth movement produced by molar uprighting spring.

INTRODUCTION: Uprighting a tipped molar by using an uprighting spring is a fundamental orthodontic treatment technique. However, mechanical analyses have not been carried out for molar uprighting, and the mechanism of tooth movement has not been clarified. The purposes of this article were to clarify these mechanisms and to demonstrate the usefulness of mechanical simulations by which the effects of many factors on tooth movement can be estimated quantitatively. METHODS: A 3-dimensional finite element method was used to simulate the uprighting of a second molar with a molar uprighting spring. The effects of a retainer and spring-arm bending on the tooth movements were shown quantitatively. RESULTS: The retainer was useful to reduce the movement of anchor teeth. The same effect could be achieved by bending the spring arm in the lingual direction, but the molar was greatly rotated in the occlusal plane and was tipped in the buccal direction. CONCLUSIONS: The usefulness of a mechanical simulation method to predict orthodontic tooth movement in clinical situations was demonstrated.

Calculation of natural frequencies of teeth supported with the periodontal ligament.

Natural frequencies and vibration modes of four kinds of teeth were calculated by using a mechanical model. The alveolar bone and the tooth were assumed as rigid bodies, while the periodontal ligament was assumed as an elastic spring. All the natural frequencies were within a range of 1 to 10 kHz. The first natural frequencies of four teeth were about 1.5 kHz, and decreased as the root length decreased. Their vibration modes were tipping movements of the root. The natural frequency of the twisting vibration mode, or rotating movement around the tooth axis, was affected by root configuration. When subjected to a periodic force, the tooth and periodontal ligament would vibrate with the corresponding resonance mode. This phenomenon may be used as a method for the diagnosis and the treatment of a periodontal tissue.

A numerical simulation of orthodontic tooth movement produced by a canine retraction spring.

Tooth movements produced by a canine retraction spring were calculated. Although a gable bend and an anti-rotational bend were incorporated into the spring, the canine tipped and rotated initially. Retraction force decreased and moment-to-force ratio increased after the spring legs closed. Then, the initial tipping and rotation began to be corrected. As a result, the canine moved almost bodily after a prolonged period of time. Such tooth movements cannot be estimated from the initial force system. The gable bend decreased tipping movement, but increased rotational movement. On the other hand, the anti-rotational bend decreased rotational movement but increased tipping movement. In other words, one bend decreased the effect of the other, when both bends were incorporated in the spring.

A numerical simulation of tooth movement by wire bending.

INTRODUCTION: In orthodontic treatment, wires are bent and attached to teeth to move them via elastic recovery. To predict how a tooth will move, the initial force system produced from the wire is calculated. However, the initial force system changes as the tooth moves and may not be used to predict the final tooth position. The purpose of this study was to develop a comprehensive mechanical, 3-dimensional, numerical model for predicting tooth movement. METHODS: Tooth movements produced by wire bending were simulated numerically. The teeth moved as a result of bone remodeling, which occurs in proportion to stress in the periodontal ligament. RESULTS: With an off-center bend, a tooth near the bending position was subjected to a large moment and tipped more noticeably than the other teeth. Also, a tooth far from the bending position moved slightly in the mesial or the distal direction. With the center V-bend, when the second molar was added as an anchor tooth, the tipping angle and the intrusion of the canine increased, and movement of the first molar was prevented. When a wire with an inverse curve of Spee was placed in the mandibular arch, the calculated tendency of vertical tooth movements was the same as the measured result. In these tooth movements, the initial force system changed as the teeth moved. Tooth movement was influenced by the size of the root surface area. CONCLUSIONS: Tooth movements produced by wire bending could be estimated. It was difficult to predict final tooth positions from the initial force system.

The effects of friction and flexural rigidity of the archwire on canine movement in sliding mechanics: a numerical simulation with a 3-dimensional finite element method.

INTRODUCTION: The purposes of this article were to clarify the combined effect of friction and an archwire's flexural rigidity on canine movement in sliding mechanics, and to explain how to select a suitable archwire and force level for efficient bodily movement. METHODS: A numerical simulation was carried out by using a 3-dimensional finite element method. RESULTS: As the frictional force decreased, both the net force acting on and the moving speed of the canine increased. The elastic deformation of the archwire increased, and the moving pattern of the canine changed from bodily movement to tipping, although there was no clearance between the archwire and the bracket slot. When a light wire was used, wire deformation increased, and the canine experienced greater tipping. These effects of friction and wire properties on canine movement (tipping and rotational angles) are combined by using a single parameter, EI/P, where EI is the flexural rigidity of the archwire, and P is the net force acting on the canine. A suitable combination of EI and P will cause canine bodily movement. The EI is determined from the archwire's size and material. CONCLUSIONS: We propose a method for estimating a suitable combination in this article.

Radiocesium removal system for environmental water and drainage.

With the development of the nuclear power generation, it is expected that severe pollution of environmental water by radiocesium (r-Cs) may occur. We developed a r-Cs removal system with a continuous stirring tank reactor (CSTR) and r-Cs adsorbent of non-woven fiber immobilizing Prussian blue nanoparticles (PBN). Results confirmed that this system can remove r-Cs from environmental water with a removal rate higher than 80% at processing speed of 2 m3/h. In this study, the processing speed and processing capacity of this system were confirmed using kinetic and equilibrium analyses of Cs adsorption behavior on PBN. The equilibrium of Cs adsorption was analyzed using a Langmuir equation. Results show that the maximum adsorption capacity was 160 mg/g (PBN). The kinetic data were well fitted using a pseudo-first order kinetic model. This rate constant was correlated to the PBN/liquid ratio in the system.