RESUMO
A novel optical-induced dielectrophoresis (ODEP) method employing a pressure-driven flow for the continuous separation of microparticles is presented in this study. By applying alternate current electric field on conductive indium tin oxide substrate and projecting the light geometry into the photoconductive layer, an inhomogeneous electric field is locally induced. The particles experience the dielectrophoretic force when passing through the lighting area, where the strongest electrical field gradient exists. By optimizing the structure of the lighting pattern, a stronger nonuniform electric field gradient is generated which predicts the separation of 1 and 3 µm polystyrene particles. Moreover, the effects of key parameters, including the light pattern geometry, applied voltage, and flow rate, were investigated in this study, leading to the successful sorting of 700 nm and 1 µm particles. To further examine the separation sensitivity and practicability of the proposed ODEP microfluidic method, the isolation of two different types of circulating tumor cells from T-cells and red blood cells are demonstrated, providing a novel method for the manipulation and separation of microparticles and nanoparticles.