RESUMO
PURPOSE: To detect protein expressions of insulin-like growth factor II messenger ribonucleic acid-binding protein 3 (IMP3) and vascular endothelial growth factor (VEGF) in osteosarcoma tissues and explore the relationships of protein expressions of IMP3 and VEGF with stage, microvessel density (MVD) and pulmonary metastasis of osteosarcoma patients. METHODS: Immunohistochemical staining was used to detect protein expressions of IMP3 and VEGF in 37 cases of osteosarcoma, and their correlations with stage, MVD and pulmonary metastasis of osteosarcoma patients were analyzed in combination with clinical data. RESULTS: Protein expressions of IMP3 and VEGF in osteosarcoma were significantly higher than those in normal tissues adjacent to the tumor (p<0.05, p<0.05). The distribution of stage III in osteosarcoma patients with high protein expressions of IMP3 and VEGF increased. With the enhancement of protein expressions of IMP3 and VEGF, MVD elevated, the probability of pulmonary metastasis increased, and the survival time decreased markedly in osteosarcoma patients. CONCLUSIONS: High expressions of IMP3 and VEGF are positively correlated with stage, MVD and pulmonary metastasis of osteosarcoma, and can be applied as potential indicators of the malignant degree of osteosarcoma and the prognosis of patients.
Assuntos
Neoplasias Pulmonares/secundário , Densidade Microvascular/genética , Osteossarcoma/genética , Fator A de Crescimento do Endotélio Vascular/metabolismo , Adolescente , Adulto , Criança , Feminino , Humanos , Masculino , Metástase Neoplásica , Ribonucleoproteínas Nucleolares Pequenas/metabolismo , Adulto JovemRESUMO
The complete mitochondrial genome of the juvenile Southern bluefin tuna Thunnus maccoyii collected from Sea of Japan was determined by next-generation sequencing. The mitogenome is a circular molecule 16,529 bp in length, including the typical structure of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. The TAS, central CSB and CSB were detected in the control region. The gene contents of the mitogenome are identical to those observed in most bony fishes.
Assuntos
Genoma Mitocondrial , Atum/genética , Animais , Códon de Iniciação , Códon de Terminação , DNA Mitocondrial/química , DNA Mitocondrial/isolamento & purificação , DNA Mitocondrial/metabolismo , Proteínas de Peixes/química , Proteínas de Peixes/genética , Sequenciamento de Nucleotídeos em Larga Escala , Fases de Leitura Aberta/genética , RNA Ribossômico/química , RNA Ribossômico/genética , RNA de Transferência/química , RNA de Transferência/genética , Análise de Sequência de DNARESUMO
The complete mitochondrial genome of the juvenile Pacific bluefin tuna Thunnus orientalis collected from Sea of Japan was determined by next-generation sequencing. The mitogenome is a circular molecule 16,529 bp in length, including the typical structure of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. The termination-associated sequence (TAS), central conserved sequence blocks (CSB) and CSB were detected in the control region. The gene contents of the mitogenome are identical to those observed in most bony fishes.
Assuntos
Genoma Mitocondrial , Atum/genética , Animais , Pareamento de Bases/genética , Sequência de Bases , DNA Mitocondrial/genética , Fases de Leitura Aberta/genética , Oceano Pacífico , RNA de Transferência/genéticaRESUMO
The complete mitochondrial genome of the juvenile Atlantic bluefin tuna Thunnus thynnus collected from Sea of Japan was determined by next-generation sequencing. The mitogenome is a circular molecule 16,529 bp in length, including the typical structure of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. The TAS, central CSB and CSB were detected in the control region. The gene contents of the mitogenome are identical to those observed in most bony fishes.