Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 24
Filtrar
Mais filtros

Base de dados
Tipo de documento
Intervalo de ano de publicação
1.
J Am Chem Soc ; 145(10): 5864-5871, 2023 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-36857108

RESUMO

In recent years, parahydrogen-induced polarization side arm hydrogenation (PHIP-SAH) has been applied to hyperpolarize [1-13C]pyruvate and map its metabolic conversion to [1-13C]lactate in cancer cells. Developing on our recent MINERVA pulse sequence protocol, in which we have achieved 27% [1-13C]pyruvate carbon polarization, we demonstrate the hyperpolarization of [1,2-13C]pyruvate (∼7% polarization on each 13C spin) via PHIP-SAH. By altering a single parameter in the pulse sequence, MINERVA enables the signal enhancement of C1 and/or C2 in [1,2-13C]pyruvate with the opposite phase, which allows for the simultaneous monitoring of different chemical reactions with enhanced spectral contrast or for the same reaction via different carbon sites. We first demonstrate the ability to monitor the same enzymatic pyruvate to lactate conversion at 7T in an aqueous solution, in vitro, and in-cell (HeLa cells) via different carbon sites. In a second set of experiments, we use the C1 and C2 carbon positions as spectral probes for simultaneous chemical reactions: the production of acetate, carbon dioxide, bicarbonate, and carbonate by reacting [1,2-13C]pyruvate with H2O2 at a high temperature (55 °C). Importantly, we detect and characterize the intermediate 2-hydroperoxy-2-hydroxypropanoate in real time and at high temperature.


Assuntos
Peróxido de Hidrogênio , Ácido Pirúvico , Humanos , Ácido Pirúvico/metabolismo , Células HeLa , Hidrogenação , Ácido Láctico
2.
Chemphyschem ; 24(2): e202200615, 2023 01 17.
Artigo em Inglês | MEDLINE | ID: mdl-36106366

RESUMO

The metabolism of malignant cells differs significantly from that of healthy cells and thus, it is possible to perform metabolic imaging to reveal not only the exact location of a tumor, but also intratumoral areas of high metabolic activity. Herein, we demonstrate the feasibility of metabolic tumor imaging using signal-enhanced 1-13 C-pyruvate-d3 , which is rapidly enhanced via para-hydrogen, and thus, the signal is amplified by several orders of magnitudes in less than a minute. Using as a model, human melanoma xenografts injected with signal-enhanced 1-13 C-pyruvate-d3, we show that the conversion of pyruvate into lactate can be monitored along with its kinetics, which could pave the way for rapidly detecting and monitoring changes in tumor metabolism.


Assuntos
Neoplasias , Ácido Pirúvico , Humanos , Ácido Pirúvico/metabolismo , Hidrogênio , Imageamento por Ressonância Magnética/métodos , Isótopos de Carbono
3.
Angew Chem Int Ed Engl ; 62(36): e202306654, 2023 09 04.
Artigo em Inglês | MEDLINE | ID: mdl-37439488

RESUMO

Metabolic magnetic resonance imaging (MRI) using hyperpolarized (HP) pyruvate is becoming a non-invasive technique for diagnosing, staging, and monitoring response to treatment in cancer and other diseases. The clinically established method for producing HP pyruvate, dissolution dynamic nuclear polarization, however, is rather complex and slow. Signal Amplification By Reversible Exchange (SABRE) is an ultra-fast and low-cost method based on fast chemical exchange. Here, for the first time, we demonstrate not only in vivo utility, but also metabolic MRI with SABRE. We present a novel routine to produce aqueous HP [1-13 C]pyruvate-d3 for injection in 6 minutes. The injected solution was sterile, non-toxic, pH neutral and contained ≈30 mM [1-13 C]pyruvate-d3 polarized to ≈11 % (residual 250 mM methanol and 20 µM catalyst). It was obtained by rapid solvent evaporation and metal filtering, which we detail in this manuscript. This achievement makes HP pyruvate MRI available to a wide biomedical community for fast metabolic imaging of living organisms.


Assuntos
Imageamento por Ressonância Magnética , Ácido Pirúvico , Imageamento por Ressonância Magnética/métodos , Solventes/química , Metanol , Água/química
4.
Anal Chem ; 94(5): 2561-2568, 2022 02 08.
Artigo em Inglês | MEDLINE | ID: mdl-35089685

RESUMO

The probing of microscopic environments by hyperpolarized xenon NMR has spurred investigations in supramolecular chemistry as well as important biosensing and molecular imaging applications. While xenon exchange with host structures at micromolar concentrations and below can be readily detected, a quantitative analysis is limited, requiring complementary experimentation by different methodologies and thus lacking completeness and compromising the validity and comparability of numerical results. Here, a new NMR measurement and data analysis approach is introduced for the comprehensive characterization of the host-xenon binding dynamics. The application of chemical exchange saturation transfer of hyperpolarized 129Xe under parametric modulation of the saturation RF amplitude and xenon gas saturation of the solution enables a delineation of exchange mechanisms and, through modeling, a numerical estimation of the various reaction rate constants (and thus magnetization exchange rate constants), the xenon affinity, and the total host molecule concentration. Only the numerical xenon solubility is additionally required for input, a quantity that has a low impact on the measurement uncertainty and is derivable from metrological data collections. Signal calibration by a reference material may thus be avoided, qualifying the method as calibration-free. For demonstration a xenon exchange with the host cucurbit[6]uril at low concentration is investigated, with the numerical results being validated by standard quantitative NMR data obtained at high concentration. The readiness to evaluate xenon exchange for the one sample at hand and in a single experimental attempt by the proposed method may allow comprehensive quantitative studies in supramolecular chemistry, biomacromolecular structure and dynamics, and sensing.


Assuntos
Imageamento por Ressonância Magnética , Xenônio , Calibragem , Espectroscopia de Ressonância Magnética/métodos , Imagem Molecular , Xenônio/química
5.
Angew Chem Int Ed Engl ; 61(34): e202206298, 2022 08 22.
Artigo em Inglês | MEDLINE | ID: mdl-35723041

RESUMO

Hyperpolarization methods in magnetic resonance enhance the signals by several orders of magnitude, opening new windows for real-time investigations of dynamic processes in vitro and in vivo. Here, we propose a field-independent para-hydrogen-based pulsed method to produce rapidly hyperpolarized 13 C-labeled substrates. We demonstrate the method by polarizing the carboxylic carbon of the pyruvate moiety in a purposely designed precursor to 24 % at ≈22 mT. Following a fast purification procedure, we measure 8 % polarization on free [1-13 C]pyruvate in clean water solutions at physiological conditions at 7 T. The enhanced signals allow real-time monitoring of the pyruvate-lactate conversion in cancer cells, demonstrating the potential of the method for biomedical applications in combination with existing or developing magnetic resonance technologies.


Assuntos
Imageamento por Ressonância Magnética , Ácido Pirúvico , Isótopos de Carbono , Hidrogênio , Imageamento por Ressonância Magnética/métodos , Espectroscopia de Ressonância Magnética/métodos , Água
6.
Analyst ; 146(5): 1772-1778, 2021 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-33475626

RESUMO

We employ Parahydrogen Induced Polarization with Side-Arm Hydrogenation (PHIP-SAH) to polarize (1-13C)-pyruvate. We introduce a new method called proton-relayed side-arm hydrogenation (PR-SAH) in which an intermediate proton is used to transfer polarization from the side-arm to the 13C-labelled site of the pyruvate before hydrolysis. This significantly reduces the cost and effort needed to prepare the precursor for radio-frequency transfer experiments while still maintaining acceptable polarization transfer efficiency. Experimentally we have attained on average 4.33% 13C polarization in an aqueous solution of (1-13C)-pyruvate after about 10 seconds of cleavage and extraction. PR-SAH is a promising pulsed NMR method for hyperpolarizing 13C-labelled metabolites in solution, conducted entirely in high magnetic field.

7.
Phys Chem Chem Phys ; 23(3): 2320-2330, 2021 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-33449978

RESUMO

The signal enhancement provided by the hyperpolarization of nuclear spins of metabolites is a promising technique for diagnostic magnetic resonance imaging (MRI). To date, most 13C-contrast agents are hyperpolarized utilizing a complex or cost-intensive polarizer. Recently, the in situ parahydrogen-induced 13C hyperpolarization was demonstrated. Hydrogenation, spin order transfer (SOT) by a pulsed NMR sequence, in vivo administration, and detection was achieved within the magnet bore of a 7 Tesla MRI system. So far, the hyperpolarization of the xenobiotic molecule 1-13C-hydroxyethylpropionate (HEP) and the biomolecule 1-13C-succinate (SUC) through the PH-INEPT+ sequence and a SOT scheme proposed by Goldman et al., respectively, was shown. Here, we investigate further the hyperpolarization of SUC at 7 Tesla and study the performance of two additional SOT sequences. Moreover, we present first results of the hyperpolarization at high magnetic field of 1-13C-phospholactate (PLAC), a derivate to obtain the metabolite lactate, employing the PH-INEPT+ sequence. For SUC and PLAC, 13C polarizations of about 1-2% were achieved within seconds and with minimal equipment. Effects that potentially may explain loss of 13C polarization have been identified, i.e. low hydrogenation yield, fast T1/T2 relaxation and the rarely considered 13C isotope labeling effect.

8.
Angew Chem Int Ed Engl ; 60(38): 20984-20990, 2021 09 13.
Artigo em Inglês | MEDLINE | ID: mdl-34289241

RESUMO

Nuclear magnetic resonance is usually drastically limited by its intrinsically low sensitivity: Only a few spins contribute to the overall signal. To overcome this limitation, hyperpolarization methods were developed that increase signals several times beyond the normal/thermally polarized signals. The ideal case would be a universal approach that can signal enhance the complete sample of interest in solution to increase detection sensitivity. Here, we introduce a combination of para-hydrogen enhanced magnetic resonance with the phenomenon of the RASER: Large signals of para-hydrogen enhanced molecules interact with the magnetic resonance coil in a way that the signal is spontaneously converted into an in-phase signal. These molecules directly interact with other compounds via dipolar couplings and enhance their signal. We demonstrate that this is not only possible for solvent molecules but also for an amino acid.

9.
Phys Chem Chem Phys ; 21(41): 22849-22856, 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31612167

RESUMO

Hyperpolarized metabolites are very attractive contrast agents for in vivo magnetic resonance imaging studies enabling early diagnosis of cancer, for example. Real-time production of concentrated solutions of metabolites is a desired goal that will enable new applications such as the continuous investigation of metabolic changes. To this end, we are introducing two NMR experiments that allow us to deliver high levels of polarization at high concentrations (50 mM) of an acetate precursor (55% 13C polarization) and acetate (17% 13C polarization) utilizing 83% para-state enriched hydrogen within seconds at high magnetic field (7 T). Furthermore, we have translated these experiments to a portable low-field spectrometer with a permanent magnet operating at 1 T. The presented developments pave the way for a rapid and affordable production of hyperpolarized metabolites that can be implemented in e.g. metabolomics labs and for medical diagnosis.


Assuntos
Técnicas de Química Analítica/métodos , Meios de Contraste/síntese química , Campos Magnéticos , Acetatos/química , Acetatos/metabolismo , Meios de Contraste/química , Hidrogênio/química , Imageamento por Ressonância Magnética/instrumentação
10.
Chemphyschem ; 2018 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-29855138

RESUMO

Cucurbit[6]uril and xenon form supramolecular complexes that are of great potential for biosensing by NMR. This host-guest system acts alike a signaler in sensors facilitating the ultrasensitive detection of biomarkers by saturation transfer of chemically exchanging, hyperpolarized 129 Xe. Here, the exchange process is evaluated by NMR exchange spectroscopy utilizing the preparation of anti-parallel longitudinal magnetization with respect to free and host-bound xenon and the variation of xenon concentration. Evidence for dissociative as well as degenerate exchange mechanisms is revealed by a linear regression analysis of the determined exchange rates resulting in rate coefficients of 1131±11 s-1 (2390±70 s-1 ) and 108500±4900 M-1 s-1 (174200±13900 M-1 s-1 ), respectively, and an affinity constant of 289±8 M-1 (278±14 M-1 ) in physiological saline at 298 K (310 K). The results elucidate the supramolecular exchange and underpin the high efficacy for biosensing of this host-guest system. The approach is generally applicable to enhanced host-xenon exchange dynamics, yet slow on the NMR timescale, for quantitative kinetics and biosensing analyses.

11.
Angew Chem Int Ed Engl ; 57(33): 10692-10696, 2018 08 13.
Artigo em Inglês | MEDLINE | ID: mdl-29923285

RESUMO

Hyperpolarization techniques are key to extending the capabilities of MRI for the investigation of structural, functional and metabolic processes in vivo. Recent heterogeneous catalyst development has produced high polarization in water using parahydrogen with biologically relevant contrast agents. A heterogeneous ligand-stabilized Rh catalyst is introduced that is capable of achieving 15 N polarization of 12.2±2.7 % by hydrogenation of neurine into a choline derivative. This is the highest 15 N polarization of any parahydrogen method in water to date. Notably, this was performed using a deuterated quaternary amine with an exceptionally long spin-lattice relaxation time (T1 ) of 21.0±0.4 min. These results open the door to the possibility of 15 N in vivo imaging using nontoxic similar model systems because of the biocompatibility of the production media and the stability of the heterogeneous catalyst using parahydrogen-induced polarization (PHIP) as the hyperpolarization method.


Assuntos
Colina/química , Hidrogênio/química , Nanopartículas Metálicas/química , Ródio/química , Água/química , Aminas/química , Catálise , Deutério/química , Hidrogenação , Isótopos de Nitrogênio/química
12.
Appl Magn Reson ; 44(1-2): 65-80, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23349565

RESUMO

A stand-alone, self-contained and transportable system for the polarization of (129)Xe by spin exchange optical pumping with Rb is described. This mobile polarizer may be operated in batch or continuous flow modes with medium amounts of hyperpolarized (129)Xe for spectroscopic or small animal applications. A key element is an online nuclear magnetic resonance module which facilitates continuous monitoring of polarization generation in the pumping cell as well as the calculation of the absolute (129)Xe polarization. The performance of the polarizer with respect to the crucial parameters temperature, xenon and nitrogen partial pressures, and the total gas flow is discussed. In batch mode the highest (129)Xe polarization of P(Xe) = 40 % was achieved using 0.1 mbar xenon partial pressure. For a xenon flow of 6.5 and 26 mln/min, P(Xe) = 25 % and P(Xe) = 13 % were reached, respectively. The mobile polarizer may be a practical and efficient means to make the applicability of hyperpolarized (129)Xe more widespread.

13.
J Chem Phys ; 137(9): 094503, 2012 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-22957577

RESUMO

Effects of spin-spin interactions on the nuclear magnetic relaxation dispersion (NMRD) of protons were studied in a situation where spin ½ hetero-nuclei are present in the molecule. As in earlier works [K. L. Ivanov, A. V. Yurkovskaya, and H.-M. Vieth, J. Chem. Phys. 129, 234513 (2008); S. E. Korchak, K. L. Ivanov, A. V. Yurkovskaya, and H.-M. Vieth, ibid. 133, 194502 (2010)], spin-spin interactions have a pronounced effect on the relaxivity tending to equalize the longitudinal relaxation times once the spins become strongly coupled at a sufficiently low magnetic field. In addition, we have found influence of (19)F nuclei on the proton NMRD, although in the whole field range, studied protons and fluorine spins were only weakly coupled. In particular, pronounced features in the proton NMRD were found; but each feature was predominantly observed only for particular spin states of the hetero-nuclei. The features are explained theoretically; it is shown that hetero-nuclei can affect the proton NMRD even in the limit of weak coupling when (i) protons are coupled strongly and (ii) have spin-spin interactions of different strengths with the hetero-nuclei. We also show that by choosing the proper magnetic field strength, one can selectively transfer proton spin magnetization between spectral components of choice.


Assuntos
Espectroscopia de Ressonância Magnética/métodos , Prótons , Compostos de Anilina/química
14.
Chemphyschem ; 12(12): 2311-21, 2011 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-21732521

RESUMO

The cis-trans isomerisation of N-benzylideneaniline (NBA) and derivatives containing a central C=N bond has been investigated experimentally and theoretically. Eight different NBA molecules in three different solvents were irradiated to enforce a photochemical trans--(hnu)-->cis isomerisation and the kinetics of the thermal backreaction cis--(Δ)-->trans were determined by NMR spectroscopy measurements in the temperature range between 193 and 288 K. Theoretical calculations using density functional theory and Eyring transition-state theory were carried out for 12 different NBA species in the gas phase and three different solvents to compute thermal isomerisation rates of the thermal back reaction. While the computed absolute rates are too large, they reveal and explain experimental trends. Time-dependent density functional theory provides optical spectra for vertical transitions and excitation energy differences between trans and cis forms. Together with isomerisation rates, the latter can be used to identify "optimal switches" with good photochromicity and reasonable thermal stability.


Assuntos
Compostos de Anilina/química , Compostos de Benzilideno/química , Físico-Química , Iminas/química , Temperatura Baixa , Isomerismo , Cinética , Espectroscopia de Ressonância Magnética , Modelos Teóricos , Processos Fotoquímicos/efeitos da radiação , Teoria Quântica , Solventes/química , Termodinâmica , Raios Ultravioleta
15.
J Chem Phys ; 133(19): 194502, 2010 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-21090863

RESUMO

Effects of scalar spin-spin interactions on the nuclear magnetic relaxation dispersion (NMRD) of coupled multispin systems were analyzed. Taking spin systems of increasing complexity we demonstrated pronounced influence of the intramolecular spin-spin couplings on the NMRD of protons. First, at low magnetic fields where there is strong coupling of spins the apparent relaxation times of the coupled spins become equal. Second, there are new features, which appear at the positions of the nuclear spin level anticrossings. Finally, in coupled spin systems there can be a coherent contribution to the relaxation kinetics present at low magnetic fields. All these peculiarities caused by spin-spin interactions are superimposed on the features in NMRD, which are conditioned by changes of the motional regime. Neglecting the effects of couplings may lead to misinterpretation of the NMRD curves and significant errors in determining the correlation times of molecular motion. Experimental results presented are in good agreement with theoretical calculations.

16.
Chem Sci ; 12(1): 314-319, 2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-34163599

RESUMO

The phenomenon of nuclear magnetic resonance (NMR) is widely applied in biomedical and biological science to study structures and dynamics of proteins and their reactions. Despite its impact, NMR is an inherently insensitive phenomenon and has driven the field to construct spectrometers with increasingly higher magnetic fields leading to more detection sensitivity. Here, we are demonstrating that enzymatic reactions can be followed in real-time at millitesla fields, three orders of magnitude lower than the field of state-of-the-art NMR spectrometers. This requires signal-enhancing samples via hyperpolarization. Within seconds, we have enhanced the signals of 2-13C-pyruvate, an important metabolite to probe cancer metabolism, in 22 mM concentrations (up to 10.1% ± 0.1% polarization) and show that such a large signal allows for the real-time detection of enzymatic conversion of pyruvate to lactate at 24 mT. This development paves the pathways for biological studies in portable and affordable NMR systems with a potential for medical diagnostics.

17.
J Phys Chem B ; 113(20): 7398-406, 2009 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-19438284

RESUMO

Time-resolved chemically induced dynamic nuclear polarization (CIDNP) was applied to the investigation of the photo-oxidation of two sulfur containing peptides, glycylmethionine (Gly-Met) and methionylglycine (Met-Gly). It was established that the reaction of Gly-Met with a photosensitizer, triplet 4-carboxybenzophenone, occurs via electron transfer from the sulfur atom and also from the terminal amino group in its uncharged state. The latter process leads to the formation of nuclear polarization of the alpha-protons of the glycine residue. The sulfur-centered cation radical of Gly-Met formed as a result of triplet quenching participates in the degenerate electron exchange reaction with the parent molecule. The rate constant of this reaction obtained from a simulation of the CIDNP kinetics is 2x10(8) M(-1) s(-1). Two channels of triplet quenching were also found for the Met-Gly peptide at pH values above the pKa of the terminal amino group: electron transfer from the amino group and from the sulfur atom. On the basis of the analysis of the CIDNP spectra and kinetics, it was found that at pH below pKa of the terminal amino group photo-oxidation of Met-Gly leads to the formation of an open-chain S-centered cation radical, which releases a proton from its N-terminal amino group to form a five-membered cyclic radical structure with a three electron bond between the S and N atoms. The rate constant of deprotonation obtained to be 1.8x10(5) s(-1) is in agreement with the pKa=4.7 of the S-centered radical of Met-Gly determined from the pH dependence of nuclear polarization. At pH>pKa, the aminium radicals formed in both peptides as a result of electron transfer from the lone pair of N-terminal amino group undergo deprotonation to the neutral aminyl radical on the submicrosecond time scale. The involvement of the different radicals was confirmed by the dependence of CIDNP on the external magnetic field ranging from 0.1 T to 7 T.


Assuntos
Dipeptídeos/química , Fótons , Água/química , Radicais Livres/química , Concentração de Íons de Hidrogênio , Cinética , Magnetismo , Processos Fotoquímicos , Soluções , Fatores de Tempo
18.
ChemistryOpen ; 7(9): 672-676, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30191091

RESUMO

para-Hydrogen-induced polarization (PHIP) is a method to rapidly generate hyperpolarized compounds, enhancing the signal of nuclear magnetic resonance (NMR) experiments by several thousand-fold. The hyperpolarization of metabolites and their use as contrast agents in vivo is an emerging diagnostic technique. High degrees of polarization and extended polarization lifetime are necessary requirements for the detection of metabolites in vivo. Here, we present pulsed NMR methods for obtaining hyperpolarized magnetization in two metabolites. We demonstrate that the hydrogenation with para-hydrogen of perdeuterated vinyl acetate allows us to create hyperpolarized ethyl acetate with close to 60 % 1H two-spin order. With nearly 100 % efficiency, this order can either be transferred to 1H in-phase magnetization or 13C magnetization of the carbonyl function. Close to 60 % polarization is experimentally verified for both nuclei. Cleavage of the ethyl acetate precursor in a 20 s reaction yields ethanol with approximately 27 % 1H polarization and acetate with around 20 % 13C polarization. This development will open new opportunities to generate metabolic contrast agents in less than one minute.

19.
ChemistryOpen ; 7(5): 344-348, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29761065

RESUMO

Diseases such as Alzheimer's and cancer have been linked to metabolic dysfunctions, and further understanding of metabolic pathways raises hope to develop cures for such diseases. To broaden the knowledge of metabolisms in vitro and in vivo, methods are desirable for direct probing of metabolic function. Here, we are introducing a pulsed nuclear magnetic resonance (NMR) approach to generate hyperpolarized metabolites within seconds, which act as metabolism probes. Hyperpolarization represents a magnetic resonance technique to enhance signals by over 10 000-fold. We accomplished an efficient metabolite hyperpolarization by developing an isotopic labeling strategy for generating precursors containing a favorable nuclear spin system to add para-hydrogen and convert its two-spin longitudinal order into enhanced metabolite signals. The transfer is performed by an invented NMR experiment and 20 000-fold signal enhancements are achieved. Our technique provides a fast way of generating hyperpolarized metabolites by using para-hydrogen directly in a high magnetic field without the need for field cycling.

SELEÇÃO DE REFERÊNCIAS
Detalhe da pesquisa