[Effect of fatty component in ration and coenzyme Q10 on indices of rat lipid metabolism in ontogenesis].

The studies made on rats of 1, 3, 6 and 12 months with fish, palm and linseed oil included in the ration in combination of KoQ10, showed that beginning from young age till 12 months fatty acid composition of liver depended of fat component in the ration. Long-term consumption of fish fat with age results in significant increase in omega 3 fatty acids. In this case the omega 6 fatty acids level remained rather high being indicative of organism adaptation and inclusion of compensatory mechanisms supporting the required level of omega 6. With rat age the content of of KoQ10 in liver of rats of the control group and animals fed by of KoQ10 and palm oil with of KoQ10 decreased by 15-27%, while the consumption of linseed oil and fish fat with tended to increase the content of KoQ10 by 30 and 35%, respectively.

[Amaranths oil: peculiarities of chemical composition and influence on lipid metabolism by rats].

In experiment on rats it was shown a hypocholesterolemic effect of amaranth oil. The decrease of cholesterol lever in rat blood was not accompanied by its increasing in a liver. The turn from a model of hypercholesterolemia to the diet with amaranth oil also prodused decreasing of cholesterol lever in rat plasma and liver as compared with control animals. In the composition of rat liver sterins the increasing of plant sterins was observed that probably was due to squalene presense in amaranth oil.

[Changes in the fatty acid composition of erythrocyte and platelet membranes after diet therapy in patients with hyperlipoproteinemia type II]. / Izmenenie sostava zhirnykh kislot membran éritrotsitov i trombotsitov pod vliianiem dietoterapii u bol'nykh giperlipoproteidemiei II tipa.

Fatty acid spectrum of blood plasma, erythrocyte, and thrombocyte membranes as well as its alterations after diet therapy, containing polyunsaturated/saturated fatty acids at the ratio of I and less than 350 mg of cholesterol per a day, was studied in patients with hereditary hypercholesterolemia using gas chromatography. Content of linoleic acid was increased and that of oleic and arachidonic acids--decreased in lipids of the patients blood plasma as compared with healthy persons; an increase of linoleic acid was also detected in thrombocyte lipids. Alterations in fatty acid spectrum of erythrocyte membranes were less pronounced. After the course of diet therapy within I month a further increase in content of linoleic acid was observed in blood plasma. Under these conditions efficiency of essential fatty acids incorporation into lipid structures of thrombocyte membranes was higher and of erythrocytes--lower in the patients as compared with healthy persons. Possible alimentary correction of fatty acid spectrum in blood lipids is discussed.

[The effect of diets containing omega-3 polyunsaturated fatty acids on biochemical indicators and the fatty acid composition of the cell membranes in patients with ischemic heart disease and hereditary hyperlipidemia]. / Vliianie diety, soderzhashchei polinenasyshchennye zhirnye kisloty semeistva omega3, na biokhimicheskie pokazateli i zhirno-kislotnyi sostav kletochnykh membran bol'nykh ishemicheskoi bolezn'iu serdtsa i semeinoi giperlipidemiei.

A decrease in content of total cholesterol, triglycerides, fibrinogen, and atherogeneity index as well as increase in fibrinolytic activity and prolongation of the hemorrhage time were observed in blood serum of 20 patients with ischemic heart disease and hyperlipidemia of the IIa and IIb types maintained on anti-atherosclerotic diet containing 20 g of ichthyenic oil within 4 weeks. Relative content of polyunsaturated fatty acids omega 6 was decreased while the content of omega 3 fatty acids was distinctly increased in erythrocytes and blood platelets membranes, thus demonstrating the high efficiency of ichthyenic oil.

[Changes in fatty acid composition of the plasma and erythrocytes in osteochondrosis]. / Izmenenie sostava zhirnykh kislot plazmy krovi i éritrotsitov pri osteokhondroze.

Alteration of fatty acid composition was studied in blood plasma and erythrocytes of 14 patients with osteochondrosis under conditions of relief-diet therapy. During the period of full starvation distinct decrease in content of linoleic and eicosatrienic acids as well as an increase in content of palmitic, oleic and arachidonic acids were detected in blood plasma. Shifts in the ratio linoleic/arachidonic acids towards an increase in content of arachidonic acid appear to occur as a result of adaptation to stress caused by hunger. These alterations in the fatty acid spectrum of blood plasma, observed during starvation, were normalized within two weeks of recreative diet therapy. Deficiency in polyunsaturated fatty acids was not found in erythrocyte membranes under conditions of the relief-diet therapy course used.

[Lipid peroxidation upon the use of omega-3 polyunsaturated fatty acids in the treatment of hypertension and ischemic heart disease]. / Perekisnoe okislenie lipidov pri ispol'zovanii v lechenii gipertonii i ishemicheskoi bolezni serdtsa polinenasyshchennykh zhirnykh kislot semeistva omega3.

Anti-atherosclerotic diet containing 10 or 20 g of ichthyenic oil, used for treatment of 29 patients with cardiovascular diseases, caused an increase in content of polyunsaturated omega 3 fatty acids species in the patients erythrocyte membranes; this pattern may be used as a sensitive criterion of evaluation of the diet fatty component effect. Despite an increase of oxidation potential substrates in tissues, elevation of malonyl dialdehyde in blood plasma and hemolysis of erythrocytes in presence of H2O2 were not observed. This suggests absence of lipid peroxidation induction in adequate consumption of antioxidants.

[Changes in lipid metabolic indices in children with chronic kidney diseases under the influence of diet therapy]. / Izmeneniia nekotorykh pokazatelei lipidnogo obmena u detei s khronicheskimi zabolevaniiami pochek pod vliianiem dietoterapii.

Variations in lipid metabolism were studied in 20 children with chronic glomerulonephritis (CGN) associated with the nephrotic syndrome and 14 children with chronic renal insufficiency given protein deficient therapeutic diets. Suggestive abnormalities of lipid metabolism involved hyperlipidemia, hypercholesterinemia and hypertriglyceridemia (more sharply pronounced in patients with CGN associated with the nephrotic syndrome) as well as hyperlipoproteidemia, chiefly of types IY and IIB. Disproportions in lipoproteid spectra of the plasma towards increase in atherogenous beta- and pre-beta-lipoproteids are characteristic for patients of both groups but sharply pronounced in CRI. These patients also show a reduced metabolization efficacy coefficient (MEC) of essential fatty acids of food to the lipid structures of erythrocyte membranes. As a results of the treatment the lipid metabolism returned to normal in most patients with CGN and in part of the patients with CRI. In order to raise the efficacy of therapeutic diets during normalization of lipid metabolism in CRI it is recommended that the fat and carbohydrate components of the diet may be changed qualitatively with due regard for the types of hyperlipoproteidemia.

[Experimental effect of the quality of various diets on blood vessel walls]. / Izuchenie vliianiia kachestvenno razlichnykh ratsionov na stenku krovenosnykh sosudov v éksperimente.

In experiments on rats it was shown that the rations containing casein (18 and 5% calorific value) and fatty component completely substituted for butter (30 and 50% calorific value), in the presence of hypersholesterolemia, hyper-beta-lipoproteinemia and hypo-alpha-lipoproteinemia, induced changes in the elasticity of the aorta, coronary and intrarenal arteries. Swelling, separation, disappearance of elasticity and tinctorial properties of elastic fibers of tunica media vasorum and internal elastic membrane were observed in the animals given the above rations during 1-6 months. In the coronary and intrarenal arteries these changes were attended by vacuolization and destruction of the medial smooth muscle cells. The vascular alterations were most early and drastically manifest in the animals that received the ration with 5% protein and 50% butter. No vascular changes were noted in the animals given sunflower oil in similar amounts and during the same period as butter, with 18% protein in the ration. Such action of sunflower oil was not pronounced when the ration contained 5% protein, large amount of cholesterol (1% of the ration mass) or trans-isomers of unsaturated fatty acids.

[Comparative study of the therapeutic effect of diets with qualitatively and quantitatively different fat content on children with cirrhosis of the liver]. / Sravnitel'naia otsenka lechebnogo deistviia diet s razlichnym v kachestvennom i kolichestvennom otnoshenii soderzhaniem zhira pri tsirrozakh pecheni u detei.

A total of 107 children with liver cirrhosis aged from 7 to 12 were kept under observation. Four types of diets with different variants of the fatty component were used in the course of their treatment. The diet No 5 ration contained a physiological set of basic nutrients (with vegetable oil comprising 12% of the total amount of fat in the ration). The diet 52 carried a higher amount of the vegetable oil with its proportion reaching 22% of the total quantity of fat at the expense of a lesser share of butter. The total amount of fat was consistent with the age-specific standard rate. The diets 53 and 54 has the amount of fat raised by 15% with respect to the physiological level through additional introduction of vegetable oil and butter "Zdorovie", respectively. The fat-enriched diets were well tolerated with no manifestations of steatorrhea in evidence. Against the background of all the diets applied the great majority of the patients demonstrated an improvement of both the clinical data (general conditions, reduction in the size of the liver) and a number of characteristics reflecting the functional capacity of the liver (normalization of the blood serum bilirubin, of proteinogram findings). Following the use of diets with an elevated fat content there could be observed a well-marked tendency toward normalization of metabolic rates. In particular the level of the blood serum cholesterol esthers was rising; the plasma and lipids of the erythrocytes stroma showed an increased proportion of arachidonic acid with simultaneous fall of the icosatrienic acid share. This may ascribed to a higher content of polyunsaturated fatty acids in the ration. The most convenient way of introducing polyunsaturated fatty acids in the ration is the dietetic butter "Zdorovie". This justifies recommending it in dietotherapy of children suffering from liver cirrhosis.

[Effect of the quality characteristics of the fatty component of the diet on the fatty acid makeup of the erythrocytic and thrombocytic membranes in healthy persons]. / Vliianie kachestvennykh osobennostei zhirovogo komponenta ratsiona na zhirnokislotnyi sostav membran éritrotsitov i trombotsitov u zdorovykh liudei.

Practically healthy males received rations in which principal sources of fat in the descending order were butter, sunflower and mustard oil. The influence of qualitative properties of the ration's fat on the lipids metabolism in the plasma and the coefficient of the metabolization effectiveness of essential fatty acids (CEM) of the food into the membranes lipids was studied. The CEM was calculated by studying the fatty acids composition of the erythrocytes and thrombocytes stroma. Inclusion in the ration of butter alone, raised the cholesterol level, a fall of the plasma phospholipids level and a significant drop of the CEM. Substitution of sunflower oil for butter led to normalization of these figures. Mustard oil had no effect. Changes in the CEM of the erythrocytes stroma proceeded slowly, significant differences having been discovered only after 6 weeks of the butter consumption. The thrombocytes CEM changed quickly, a stable fatty acids composition of thrombocytes with this ration being established by the end of the 2nd week. The qualitative properties of the ration's fat exercise a sufficiently strong influence on the effective metabolization of fatty acids in the food into the lipid structures of the erythrocytes and thrombocytes membranes in a healthy subject, it being characterized by the CEM changes.

[Effect of diets of different isomer composition of fatty acids on the blood vessel wall, blood lipids and platelet aggregation]. / Vliianie ratsionov s razlichnym izomernym sostavom zhirnykh kislot na stenku krovenosnykh sosudov, lipidy krovi i agregatsiiu trombotsitov.

It was established in rat experiments that prolonged (4.5 months) use of the diet with a high content of spatial (trans-) and positional isomers of fatty acids exerted an adverse effect on the laboratory animals. They showed the impairment of the hemostasis integrity; namely of the walls of blood vessels (aorta, intraorgan arteries of the heart and kidneys), blood lipids, fatty acid composition and aggregation properties of platelets. The animals developed alterations similar to those occurring with the use of the diets rich in saturated fatty acids. The vessels manifested dystrophic alterations of elasticity and smooth muscle cells, whereas blood an increase in the content of atherogenic lipoproteins associated with a high content of total lipids and cholesterol. The ADP-induced enhancement of platelet aggregation was observed in experiments in vitro.

[Effects of anti-atherosclerosis diet containing omega 3 fatty acids on the lipid spectrum of blood and cell membranes in patients with ischemic heart disease and essential hypertension]. / Vliianie protivoateroskleroticheskoi diety, soderzhashchei zhirnye kisloty semeistva omega 3, na lipidnyi spektr syvorotki krovi i kletochnykh membran u bol'nykh ishemicheskoi bolezn'iu serdtsa i gipertonicheskoi bolezn'iu.

A total of 27 patients received an anti-atherosclerotic diet containing fatty fish Greenland halibut, 100 g/day. Significant reduction of the total cholesterol level, cholesterol levels of very low and low density proteins, triglycerides, atherogenic index, elevation of blood fibrinolytic activity in the presence of lowered levels of arachidonic acid and an increased relative content of omega 3 polyunsaturated fatty acids (PUFA) were recorded in membranes of red blood cells and platelets. The results of the investigations conducted have shown that even rather low doses of omega 3 PUFA introduced in the presence of the hypolipidemic diet are conductive to the alimentary correction of the blood serum lipid spectrum of the fatty-acid content in cellular membrane structural lipids.

[Optimization of dietary fat composition in erosive and ulcerative diseases of the gastroduodenal area]. / Optimizatsiia zhirovoi chasti ratsiona pri érozivno-iazvennykh porazheniiakh gastroduodenal'noi zony.

Fish oil preparation "Polyen" was used for treatment 21 patients with ulcerative diseases of the stomach or duodenum. The cicatrization of ulcer was diagnosed in 85% of patients treated by "Polyen" and in 60% of those who did not take fish oil. "Polyen" influenced fatty acid composition of erythrocyte membranes and lipid peroxidation. Authors draw a conclusion that omega-3 PUFA's can stimulate the reparative processes.

[Clinico-metabolic effects of fish oil in patients with ischemic heart disease, familial hyperlipoproteinemia and hypertension]. / Izuchenie kliniko-metabolicheskikh éffektov ikhtienovogo masla u bol'nykh ishemicheskoi bolezn'iu serdtsa, semeinoi giperlipoproteidemiei, gipertonicheskoi bolezn'iu.

Fifty-five patients with ischemic heart disease, familial hyperlipoproteinemia type IIb and essential hypertension kept anti-atherosclerosis diet incorporating 20 g of ichthyenic oil for 4 weeks. The diet resulted in positive shifts in clinical manifestations, a fall in blood levels of total cholesterol, triglycerides, atherogenic coefficient. The clotting time and duration of hemorrhage proved longer. As to fatty acid composition of red cell and platelet membranes, proportion of omega-6 reduced, while the share of omega-3 rose. This may underly inhibited synthesis of PGE2 and PGF2 alpha noted in the examinees.

[Use of the fish oil "Polyen" in pediatric practice]. / Opyt primeneniia ryb'ego zhira "polien" v pediatricheskoi praktike.

The effects of supplementation of basic diets with 'Polyen' in daily doses of 2.5-4.0 g during 30-40 days together with antioxidants were studied in patients with kidney diseases (diet No7, n = 14), different allergies (hypoallergenic diet, n = 37) and in control children (n = 12). It was shown positive dynamics in fatty acid contents in plasma and red blood cell membranes, in humoral and cell immunity indicators and in improving of clinical symptoms of diseases. 'Polyen' is recommended for wide use in pediatric practice.

[Effect of nutrition on the structural and functional organization of thrombocytes]. / Vliianie pitaniia na strukturno-funktsional'nuiu organizatsiiu trombotsitov.

Measurements were made of the composition of fatty acids contained by platelet lipids in practically healthy persons depending on the diets. Both the amount of essential fatty acids in the diet and their appurtenance to the omega-6 or omega-3 families were established to influence the composition of platelet fatty acids. The conclusion is drawn about the necessity of taking into consideration that diet influence while using platelet as a test system for estimating physiological and pathological conditions of the body.

[The effect of the omega 6/omega 3 PUFA ratios in the rations of rats on the eicosanoid content in the blood plasma and liver]. / Vliianie sootonoshenii PNZhK omega 6/omega 3 v ratsionakh krys na soderzhanie éikozanoidov v plazme krovi i pecheni.

Purified diets for rats contained 24% fat, with presented with the mixture of sunflower and fish oils whose combination yielded ratios of omega 6 to omega 3 fatty acids equal to 99 0, 49 0, 5 2, 1.9 and 0.12. The alterations in fatty acid composition of blood plasma and the liver were noticed, which manifested in increased omega 3 fatty acid levels in proportion to their dietary content. The elevation of PUFA proportion in the diet increased TBA-reactive substances in the liver. Using radioimmunoassay the levels of PGF2 alpha, TxA2 and PGI2 were determined. The decrease of arachidonic acid as part of tissue lipids caused an inhibition of PGF2 alpha formation in the liver, TxA2 and PGI2 in blood plasma, that showed a possibility of influence the ratio omega 6/omega 3 PUFA in a diet on eicosanoid synthesis by substrate regulation. These results suggest that for characteristics of dietary fat the ratio omega 6/omega 3 PUFA as a criterion reflecting the biological action of two families of essential fatty acids should not be used.