Genetic polymorphism and population structure of Echinococcus ortleppi.

The zoonotic cestode Echinococcus ortleppi (Lopez-Neyra and Soler Planas, 1943) is mainly transmitted between dogs and cattle. It occurs worldwide but is only found sporadically in most regions, with the notable exception of parts of southern Africa and South America. Its epidemiology is little understood and the extent of intraspecific variability is unknown. We have analysed in the present study the genetic diversity among 178 E. ortleppi isolates from sub-Saharan Africa, Europe and South America using the complete mitochondrial cox1 (1608 bp) and nad1 (894 bp) DNA sequences. Genetic polymorphism within the loci revealed 15 cox1 and six nad1 haplotypes, respectively, and 20 haplotypes of the concatenated genes. Presence of most haplotypes was correlated to geographical regions, and only one haplotype had a wider spread in both eastern and southern Africa. Intraspecific microvariance was low in comparison with Echinococcus granulosus sensu stricto, despite the wide geographic range of examined isolates. In addition, the various sub-populations showed only subtle deviation from neutrality and were mostly genetically differentiated. This is the first insight into the population genetics of the enigmatic cattle adapted Echinococcus ortleppi. It, therefore, provides baseline data for biogeographical comparison among E. ortleppi endemic regions and for tracing its translocation paths.

Hazard analysis of Arid and semi-Arid (ASAL) regions of Kenya.

INTRODUCTION: This paper describes a situationanalysis on hazards in the Arid and semi-Arid lands of Kenya. The leading hazards affecting the Arid and semi-arid lands are mainly natural and include among others drought, floods, and landslides. Other hazards of importance were found to be war and conflict, HIV/AIDS and fires. Over 80% of these are weather related. OBJECTIVES: The overall objective of this study was to prioritize hazards in the ASAL region. Specifically, the study identified the top ten hazards in the ASAL Districts of Kenya, determined Probability of occurrence; Analyzed the potential impact of the hazard and utilizing multiplier effect prioritized the Hazards using a hypothetical model. METHODOLOGY: This was a descriptive study conducted in over half of the Kenya's ASAL Districts in four regions of Lower and Upper Eastern, North Eastern and part of the Coast region. Six Districts were purposively selected per region with six officers from each District all totaling one hundred and forty four. The sectors where respondents were sourced from were Agriculture, Health, local Government, and Provincial Administration, Environment and NGO. The members through a consensus process analyzed hazards in groups of their respective districts using a tool that had been developed and respondents trained on its use. RESULTS: One hundred and forty four (144) officers from Twenty four Districts in the four regions were recruited. One hundred twenty seven (81%) were male and only 27 (19% ) were female The representation of participants per sector was Governance 25% followed by Civil society organizations 21%, Health 16%, Agriculture and arid lands 15%, Research and scientific institutions 13%. The top Priority Hazards identified using the mean score were Drought and famine (5.4) Epidemics and epizootics (3.8), HIV/AIDS (3.6), War and conflict (2.5), Floods (2.5) CONCLUSIONS: The exercise confirmed the priority hazards in the Arid and semi-arid regions of Kenya and described vulnerability factors that included water scarcity, poverty and low educational levels. The region suffers from a variety of hazards in particular Drought and famine, Epidemics including HIV/AIDS and War and conflict. Environmental degradation though given a low score may be more of a perception. There is need to undertake a comprehensive hazard and Vulnerability analysis at regional and country level to inform interventions and other developmental activities. Women should be targeted at the community and leadership level, and efforts to empower them should be stepped up.

Prevalence of Echinococcus granulosus in some selected sites of Ethiopia.

This study assesses the current prevalence status of Cystic echinococcosis in cattle and sheep and echinococcosis in dogs in Assela, Makale and Debre Zeit areas of Ethiopia. Our target areas represent three different agro-ecological zones in Ethiopia. Of the cattle studied the infected were, 17(13.4%) in Assela, 20(23.3%) in Makale, and 3(10%) in Debre Zeit haboured at least one fertile Hydatid cyst. Among the sheep, 23(59%) haboured fertile cysts and no goat was found to have fertile cysts. There was a significant difference in the percentage of infected sheep with fertile cysts from the same group of cattle originating from the three study areas. The percentage fertility of the total cysts examined were 4.2%, 6.8% and 5.5% in Assela, Makale and Debre Zeit respectively. Out of this 56.5% was from Assela, 65.4%, Makale and 66.7% from Debre Zeit were viable. From the sheep examined, 43.6% had fertile cysts with viability of 90.7%. The lung was the most affected organ in both species. A total of 44 dogs, 14 from Assela, 15 from Makale, and 15 from Debre Zeit were examined for canine echinococcosis. The prevalence of the infection was 7(50%) in Assela, 3(20%) in Makale and 5(33.3%) in Debre Zeit. There was no significant difference between these prevalence rates although noticeable differences in the worm burdens among the three areas were observed.

Effectiveness of incinerators in the management of medical wastes in hospitals within Eldoret municipality.

INTRODUCTION AND OBJECTIVES: Medical waste incinerators release into the air a host of pollutants that have serious adverse consequences on public health and the environment. This study aimed at determining the effectiveness of incinerators in Eldoret municipality in the management of medical wastes in relation to its maintenance and operation with an aim of giving recommendations towards reducing environmental pollution caused by emission of toxic pollutants and safeguarding the health of the incinerator operators as well as the surrounding communities. METHODOLOGY: A cross sectional study was carried out at three health facilities in Eldoret Municipality namely Moi Teaching and Referral Hospital blood Bank Transfusion Centre, Elgon View and Eldoret hospitals. Questionnaires, researcher observation and laboratory investigations of ash samples were used in data collection. The questionnaires were administered to all the personnel operating the incinerators. The ash samples collected were analyzed using Atomic Absorption Spectroscopy. RESULTS: The results showed that the incinerators were operated in substandard conditions due to breakdown of the ignition starter, lack of pollution control equipment, and a low combustion temperature. Laboratory investigation of the ash showed that the levels of the heavy metals tested were high compared to the national and international standards. Forty percent (40%) of the interviewees were aware of the health risks they were exposed to and used protective clothing and equipment. CONCLUSIONS: This study showed that the incinerators investigated are maintained and operated in substandard conditions. The ashes produced contain a wide range of toxic pollutants, including concentrated levels of a number of heavy metals. This poses serious environmental health and occupational safety hazard.

Taxonomic position and geographical distribution of the common sheep G1 and camel G6 strains of Echinococcus granulosus in three African countries.

The taxonomic and phylogenetic status of Echinococcus granulosus strains are still controversial and under discussion. In the present study, we investigated the genetic polymorphism of E. granulosus isolates originating from three countries of Africa, including a region of Algeria, where the common G1 sheep and the camel G6 strains coexist sympatrically. Seventy-one hydatid cysts were collected from sheep, cattle, camels, and humans. Two mitochondrial markers (cox1 and nad1) were used for strain identification. Two nuclear markers (actII and hbx2) were used to study the possible occurrence of cross-fertilization. Despite the heterogeneity observed among the G1 isolates, they were all localized within one robust cluster. A second strong cluster was also observed containing all of the G6 isolates. Both strains appeared as two distinct groups, and no cases of interbreeding were found. Thus, the attribution of a species rank can be suggested. We also found the Tasmanian sheep G2 strain for the first time in Africa. Because of the slight variations observed between the common sheep and the Tasmanian sheep strains, further studies should be carried out to elucidate the epidemiological relevance of this genetic discrimination.