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1.
J Pharmacol Exp Ther ; 349(1): 75-84, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24492651

RESUMO

Nasal congestion is one of the most troublesome symptoms of many upper airways diseases. We characterized the effect of selective α2c-adrenergic agonists in animal models of nasal congestion. In porcine mucosa tissue, compound A and compound B contracted nasal veins with only modest effects on arteries. In in vivo experiments, we examined the nasal decongestant dose-response characteristics, pharmacokinetic/pharmacodynamic relationship, duration of action, potential development of tolerance, and topical efficacy of α2c-adrenergic agonists. Acoustic rhinometry was used to determine nasal cavity dimensions following intranasal compound 48/80 (1%, 75 µl). In feline experiments, compound 48/80 decreased nasal cavity volume and minimum cross-sectional areas by 77% and 40%, respectively. Oral administration of compound A (0.1-3.0 mg/kg), compound B (0.3-5.0 mg/kg), and d-pseudoephedrine (0.3 and 1.0 mg/kg) produced dose-dependent decongestion. Unlike d-pseudoephedrine, compounds A and B did not alter systolic blood pressure. The plasma exposure of compound A to produce a robust decongestion (EC(80)) was 500 nM, which related well to the duration of action of approximately 4.0 hours. No tolerance to the decongestant effect of compound A (1.0 mg/kg p.o.) was observed. To study the topical efficacies of compounds A and B, the drugs were given topically 30 minutes after compound 48/80 (a therapeutic paradigm) where both agents reversed nasal congestion. Finally, nasal-decongestive activity was confirmed in the dog. We demonstrate that α2c-adrenergic agonists behave as nasal decongestants without cardiovascular actions in animal models of upper airway congestion.


Assuntos
Agonistas de Receptores Adrenérgicos alfa 2/farmacologia , Descongestionantes Nasais/farmacologia , Receptores Adrenérgicos alfa 2/metabolismo , Rinite Vasomotora/tratamento farmacológico , Administração Intranasal , Administração Oral , Agonistas de Receptores Adrenérgicos alfa 2/administração & dosagem , Agonistas de Receptores Adrenérgicos alfa 2/farmacocinética , Agonistas de Receptores Adrenérgicos alfa 2/uso terapêutico , Antagonistas de Receptores Adrenérgicos alfa 2/farmacologia , Animais , Gatos , Modelos Animais de Doenças , Cães , Relação Dose-Resposta a Droga , Técnicas In Vitro , Masculino , Descongestionantes Nasais/administração & dosagem , Descongestionantes Nasais/farmacocinética , Descongestionantes Nasais/uso terapêutico , Mucosa Nasal/irrigação sanguínea , Mucosa Nasal/efeitos dos fármacos , Mucosa Nasal/metabolismo , Rinite Vasomotora/metabolismo , Suínos , Vasoconstrição/efeitos dos fármacos
2.
Auton Neurosci ; 117(1): 17-24, 2005 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-15620566

RESUMO

The clonidine mydriasis model in rats has been widely applied in preclinical research to characterize alpha(2)-adrenoceptor antagonistic properties of drugs. The present study was undertaken to pharmacologically determine if imidazoline I(1) receptors are also involved in this model system. Sigmoid dose-response curves for pupillary dilation were produced in pentobarbital anesthetized rats by intravenous administration of increasing doses of agonists (guanabenz for alpha(2)-adrenoceptors, clonidine for both alpha(2)-adrenoceptors and imidazoline I(1) receptors, and rilmenidine for imidazoline I(1) receptors). Two antagonists (RS 79948 for alpha(2)-adrenoceptors and efaroxan for imidazoline I(1) receptors) were used to antagonize the mydriasis elicited by those three agonists, with antagonistic potencies calculated. In additional experiments, we examined the effect of the selective imidazoline I(1) receptor antagonist, AGN 192403, on clonidine-induced mydriasis. The results showed that pupillary response curves elicited by guanabenz, clonidine and rilmenidine were competitively antagonized by both RS 79948 (0.03-1 mg/kg) and efaroxan (0.03-1 mg/kg) in a dose-related fashion. The potencies of either antagonist against the three agonists were not significantly different. AGN 192403 (5 mg/kg) did not significantly shift the clonidine mydriasis curve. These results suggest that imidazoline I(1) receptors are not functionally involved in the rat clonidine mydriasis model and support this in vivo system as a useful model for studies of alpha(2)-adrenoceptors.


Assuntos
Clonidina , Midríase/metabolismo , Receptores de Droga/fisiologia , Agonistas alfa-Adrenérgicos , Antagonistas Adrenérgicos alfa/farmacologia , Animais , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Interações Medicamentosas , Receptores de Imidazolinas , Masculino , Midríase/induzido quimicamente , Fenoxibenzamina/farmacologia , Pupila/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley
3.
J Appl Physiol (1985) ; 92(2): 617-21, 2002 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11796672

RESUMO

Experiments were undertaken to characterize a noninvasive chronic, model of nasal congestion in which nasal patency is measured using acoustic rhinometry. Compound 48/80 was administered intranasally to elicit nasal congestion in five beagle dogs either by syringe (0.5 ml) in thiopental sodium-anesthetized animals or as a mist (0.25 ml) in the same animals in the conscious state. Effects of mast cell degranulation on nasal cavity volume as well as on minimal cross-sectional area (A(min)) and intranasal distance to A(min) (D(min)) were studied. Compound 48/80 caused a dose-related decrease in nasal cavity volume and A(min) together with a variable increase in D(min). Maximal responses were seen at 90-120 min. Compound 48/80 was less effective in producing nasal congestion in conscious animals, which also had significantly larger basal nasal cavity volumes. These results demonstrate the utility of using acoustic rhinometry to measure parameters of nasal patency in dogs and suggest that this model may prove useful in studies of the actions of decongestant drugs.


Assuntos
Anestesia , Cavidade Nasal/fisiopatologia , Obstrução Nasal/fisiopatologia , Ventilação Pulmonar , Animais , Cães , Relação Dose-Resposta a Droga , Masculino , Obstrução Nasal/induzido quimicamente , Ventilação Pulmonar/efeitos dos fármacos , Valores de Referência , Rinometria Acústica , Fatores de Tempo , p-Metoxi-N-metilfenetilamina/administração & dosagem , p-Metoxi-N-metilfenetilamina/farmacologia
4.
Eur J Pharmacol ; 440(1): 53-9, 2002 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-11959088

RESUMO

The aim of this study was to determine the relative contribution of alpha(1)- and alpha(2)-adrenoceptors involved in sympathetic-evoked vasoconstrictor responses in tissues perfused by the lingual arterial circulation in pentobarbital anesthetized cats. Blood flow in the lingual artery was measured by ultrasonic flowmetry. Laser-Doppler flowmetry was utilized to measure oral tissue vasoconstrictor responses in the maxillary gingiva and from the surface of the tongue. Electrical stimulation of the preganglionic superior cervical sympathetic nerve resulted in frequency-dependent blood flow decreases at all three sites. These responses were stable over time and were uniformly antagonized by administration of phentolamine (0.3 - 3.0 mg kg(-1)). The selective alpha(1)-adrenoceptor antagonist, prazosin (10 - 300 microg kg(-1)), attenuated vasoconstriction in the lingual artery and gingiva, but was ineffective in blocking vasoconstriction in the tongue. Subsequent administration of rauwolscine (300 microg kg(-1)) antagonized remaining vasoconstrictor responses. In contrast, rauwolscine (10 - 300 microg kg(-1)), given alone, blocked evoked vasoconstriction in the tongue, and was without effect on gingival or lingual artery vasoconstrictor responses. Subsequent administration of prazosin (300 microg kg(-1)) largely antagonized remaining neurally elicited responses. These results suggest that neural vasoconstrictor responses in some regional vascular beds in the cat oral cavity are mediated by both alpha(1)- and alpha(2)-adrenoceptors. In contrast, tongue surface vasoconstrictor responses to sympathetic nerve activation appear to be mediated primarily by alpha(2)-adrenoceptors.


Assuntos
Artérias/fisiologia , Gengiva/irrigação sanguínea , Receptores Adrenérgicos alfa/fisiologia , Língua/irrigação sanguínea , Antagonistas Adrenérgicos alfa/farmacologia , Animais , Artérias/efeitos dos fármacos , Artérias/inervação , Velocidade do Fluxo Sanguíneo , Gatos , Relação Dose-Resposta a Droga , Estimulação Elétrica , Feminino , Gengiva/inervação , Masculino , Fentolamina/farmacologia , Prazosina/farmacologia , Receptores Adrenérgicos alfa/efeitos dos fármacos , Simpatectomia , Sistema Nervoso Simpático/fisiologia , Sistema Nervoso Simpático/cirurgia , Língua/inervação , Vasoconstrição/efeitos dos fármacos , Ioimbina/farmacologia
5.
Eur J Pharmacol ; 471(2): 135-40, 2003 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-12818701

RESUMO

Previously, we reported that the alpha(1A)-adrenoceptor, but not the alpha(1D)-adrenoceptor, mediates pupillary dilation elicited by sympathetic nerve stimulation in rats. This study was undertaken to further characterize the alpha-adrenoceptor subtypes mediating pupillary dilation in response to both neural and agonist activation. Pupillary dilator response curves were generated by intravenous injection of norepinephrine in pentobarbital-anesthetized rats. Involvement of alpha(1)-adrenoceptors was established as mydriatic responses were inhibited by systemic administration of nonselective alpha-adrenoceptor antagonists, phentolamine (0.3-3 mg/kg) and phenoxybenzamine (0.03-0.3 mg/kg), as well as by the selective alpha(1)-adrenoceptor antagonist, prazosin (0.3 mg/kg). The alpha(2)-adrenoceptor antagonist, rauwolscine (0.5 mg/kg), was without antagonistic effects. alpha(1A)-Adrenoceptor selective antagonists, 2-([2,6-dimethoxyphenoxyethyl]aminomethyl)-1,4-benzodioxane (WB-4101; 0.1-1 mg/kg) and 5-methylurapidil (0.1-1 mg/kg), the alpha(1B)-adrenoceptor selective antagonist, 4-amino-2-[4-[1-(benzyloxycarbonyl)-2(S)- [[(1,1-dimethylethyl)amino]carbonyl]-piperazinyl]-6,7-dimethoxyquinazoline (L-765314; 0.3-1 mg/kg), as well as the alpha(1D)-adrenoceptor selective antagonist, 8-[2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl]-8-azaspiro[4.5]decane-7,9-dione (BMY-7378; 1 mg/kg), were used to delineate the adrenoceptor subtypes involved. Mydriatic responses to norepinephrine were significantly antagonized by intravenous administration of both WB-4101 and 5-methylurapidil, but neither by L-765314 nor by BMY-7378. L-765314 (0.3-3 mg/kg, i.v.) was also ineffective in inhibiting the mydriasis evoked by cervical sympathetic nerve stimulation. These results suggest that alpha(1B)-adrenoceptors do not mediate sympathetic mydriasis in rats, and that the alpha(1A)-adrenoceptor is the exclusive subtype mediating mydriatic responses in this species.


Assuntos
Midríase/induzido quimicamente , Prazosina/análogos & derivados , Pupila/efeitos dos fármacos , Receptores Adrenérgicos alfa 1/efeitos dos fármacos , Receptores Adrenérgicos alfa 1/fisiologia , Fibras Adrenérgicas/efeitos dos fármacos , Fibras Adrenérgicas/fisiologia , Antagonistas Adrenérgicos alfa/administração & dosagem , Antagonistas Adrenérgicos alfa/farmacocinética , Animais , Dioxanos/administração & dosagem , Dioxanos/farmacocinética , Relação Dose-Resposta a Droga , Estimulação Elétrica , Injeções Intravenosas , Masculino , Midríase/prevenção & controle , Norepinefrina/administração & dosagem , Norepinefrina/farmacocinética , Fenoxibenzamina/administração & dosagem , Fenoxibenzamina/farmacocinética , Fentolamina/administração & dosagem , Fentolamina/farmacocinética , Piperazinas/administração & dosagem , Piperazinas/farmacocinética , Prazosina/administração & dosagem , Prazosina/farmacocinética , Ratos , Ratos Sprague-Dawley , Ioimbina/administração & dosagem , Ioimbina/farmacocinética
6.
Eur J Pharmacol ; 489(3): 207-13, 2004 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-15087245

RESUMO

Serotonin (5-HT)(1A) receptor agonists have been reported to produce mydriasis in mice, and miosis in rabbits and humans. However, the underlying mechanisms for this action are unclear. This study was undertaken in an attempt to explore the mechanism by which 5-HT(1A) receptors are involved in the modulation of pupillary size in pentobarbital-anesthetized rats. Intravenous administration of the 5-HT(1A) receptor agonist, (2R)-(+)-8-hydroxy-2-(di-n-propylamino)tetralin hydrobromide (8-OH-DPAT; 0.003-3 mg/kg), elicited dose-dependent pupillary dilation, which was not affected by section of the preganglionic cervical sympathetic nerve. 8-OH-DPAT-elicited mydriatic responses were attenuated by the selective 5-HT(1A) receptor antagonist, N-[2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl]-N-2-pyridinylcyclohexanecarboxamide maleate (WAY 100635; 0.3-1 mg/kg, i.v.), as well as by the selective alpha(2)-adrenoceptor antagonist, (8aR,12aS,13aS)-5,8,8a,9,10,11,12,12a,13,13a-dechydro-3-methoxy-12-(ethylsulfonyl)-6H-isoquino[2,1-g][1,6]naphthyridine hydrochloride (RS 79948; 0.3 mg/kg, i.v.), but not by the selective alpha(1)-adrenoceptor antagonist, prazosin (0.3 mg/kg, i.v.). Mydriatic responses elicited by the alpha(2)-adrenoceptor agonist, guanabenz (0.003-0.3 mg/kg, i.v.), were not antagonized by WAY 100635 (0.3-1 mg/kg, i.v.). To determine whether central nervous system (CNS) 5-HT(1A) receptors, like alpha(2)-adrenoceptors, are involved in reflex mydriasis, voltage response curves of pupillary dilation were constructed by stimulation of the sciatic nerve in anesthetized rats. WAY 100635 (1 mg/kg, i.v.) did not antagonize the evoked reflex mydriasis, which, however, was blocked by RS 79948 (0.3 mg/kg, i.v.). Taken together, these results suggest that 8-OH-DPAT produces pupillary dilation in anesthetized rats by stimulating CNS 5-HT(1A) receptors, which in turn trigger the release of norepinephrine, presumably from the locus coeruleus. The latter reduces parasympathetic neuronal tone to the iris sphincter muscle by stimulation of postsynaptic alpha(2)-adrenoceptors within the Edinger-Westphal nucleus. Unlike alpha(2)-adrenoceptors, 5-HT(1A) receptors in the CNS do not mediate reflex mydriasis evoked by sciatic nerve stimulation.


Assuntos
8-Hidroxi-2-(di-n-propilamino)tetralina/farmacocinética , Midríase/induzido quimicamente , 8-Hidroxi-2-(di-n-propilamino)tetralina/administração & dosagem , 8-Hidroxi-2-(di-n-propilamino)tetralina/antagonistas & inibidores , Antagonistas de Receptores Adrenérgicos alfa 2 , Animais , Relação Dose-Resposta a Droga , Estimulação Elétrica , Guanabenzo/farmacologia , Injeções Intravenosas , Isoquinolinas/farmacologia , Masculino , Midríase/prevenção & controle , Naftiridinas/farmacologia , Piperazinas/farmacologia , Pupila/efeitos dos fármacos , Pupila/fisiologia , Piridinas/farmacologia , Ratos , Ratos Sprague-Dawley , Receptor 5-HT1A de Serotonina/administração & dosagem , Receptor 5-HT1A de Serotonina/fisiologia , Receptores Adrenérgicos alfa 2/administração & dosagem , Receptores Adrenérgicos alfa 2/fisiologia , Nervo Isquiático/patologia , Nervo Isquiático/fisiologia , Antagonistas do Receptor 5-HT1 de Serotonina
7.
J Pharmacol Toxicol Methods ; 47(1): 11-7, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12387934

RESUMO

INTRODUCTION: The present experiments were undertaken to pharmacologically characterize a noninvasive, chronic, experimental dog model of nasal congestion with the overall goal of developing an effective tool for studying the mechanism of action of nasal decongestant drugs. METHODS: Nasal patency was measured using acoustic rhinometry with chlorpheniramine and d-pseudoephedrine used as test agents. Solubilized compound 48/80 was administered as an intranasal mist to a single naris, to elicit nasal congestion in five conscious beagle dogs. Effects of localized degranulation of mast cells on nasal cavity volume, with and without pretreatment with oral decongestant drugs, were measured before and after compound 48/80 administration. Each series of experiments were repeated with a minimum 2-week rest period between trials. RESULTS: Compound 48/80 caused a decrease of nasal cavity volume (to about 50% of control). Maximal responses were seen at 90-120 min after 48/80 administration and were of similar magnitude when trials were repeated. Oral administration of the adrenergic agonist, d-pseudoephedrine (3 mg/kg), as well as the histamine H(1) receptor antagonist, chlorpheniramine (10 mg/kg), reduced compound 48/80-induced nasal congestion with the greater effect seen with alpha-adrenoceptor blockade. DISCUSSION: These results demonstrate the utility of using acoustic rhinometry to measure parameters of nasal patency in the conscious dog, and suggest that this model may provide an effective tool with which to study the actions of decongestant drugs in preclinical investigations using conscious preparations. As this technology is noninvasive, replicate determinations can be made in the same experimental subjects. Both alpha-adrenoceptor agonism and, to a lesser extent, histamine H(1) receptor antagonism appear to block compound 48/80-induced nasal congestion in this model.


Assuntos
Obstrução Nasal/fisiopatologia , Otolaringologia/métodos , Administração Oral , Administração Tópica , Animais , Clorfeniramina/administração & dosagem , Clorfeniramina/uso terapêutico , Modelos Animais de Doenças , Cães , Quimioterapia Combinada , Efedrina/administração & dosagem , Efedrina/uso terapêutico , Antagonistas dos Receptores Histamínicos H1/administração & dosagem , Antagonistas dos Receptores Histamínicos H1/uso terapêutico , Masculino , Obstrução Nasal/tratamento farmacológico , p-Metoxi-N-metilfenetilamina/administração & dosagem , p-Metoxi-N-metilfenetilamina/farmacologia
8.
J Ocul Pharmacol Ther ; 18(2): 115-25, 2002 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-12002665

RESUMO

A new technique using ultrasonic flowmetry was developed in order to directly measure blood flow in the long posterior ciliary artery (LPCA) of anesthetized cats. Basal LPCA blood flow averaged about 0.6 ml/min and was stable over the experimental period. Electrical stimulation of the cervical preganglionic cervical sympathetic nerve produced frequency-dependent anterior segment ocular vasoconstrictor responses. Ipsilateral nictitating membrane contractions were simultaneously measured as a well-established index of neural sympathetic activation. LPCA frequency-response relationships were shifted to the right in comparison with those for the nictitating membrane. When elicited at two min intervals, submaximal evoked responses of both systems were stable for more than 90 min. Ocular vasoconstrictor and nictitating membrane responses were blocked in a dose-dependent fashion by intravenous treatment with the non-selective a-adrenoceptor antagonist, phentolamine (0.3-3.0 mg/kg), as well as with the selective alpha1-adrenoceptor antagonist, prazosin (3-30 microg/kg). In contrast, neither evoked response was further antagonized by subsequent administration of the alpha2-adrenoceptor antagonist, yohimbine (500 microg/kg). These results demonstrate the usefulness of ultrasonic flowmetry to study mechanisms controlling ocular anterior segment circulation and suggest that, as previously established for the nictitating membrane and anterior choroid, adrenergic neurogenic vasoconstriction in tissues perfused by the LPCA is mediated predominantly by alpha1-adrenoceptors.


Assuntos
Corpo Ciliar/irrigação sanguínea , Antagonistas Adrenérgicos alfa/farmacologia , Animais , Artérias/diagnóstico por imagem , Artérias/fisiologia , Gatos , Estimulação Elétrica , Feminino , Masculino , Membrana Nictitante/fisiologia , Fentolamina/farmacologia , Prazosina/farmacologia , Fluxo Sanguíneo Regional/fisiologia , Sistema Nervoso Simpático/fisiologia , Ultrassonografia , Ioimbina/farmacologia
9.
J Ocul Pharmacol Ther ; 20(6): 479-88, 2004 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-15684808

RESUMO

The aim of this study was to identify receptors that mediate reflex mydriasis in pentobarbital-anesthetized rabbits, in which the cervical sympathetic nerve was sectioned unilaterally. Voltage-response curves of pupillary dilation were generated bilaterally by stimulation of the sciatic nerve. Evoked mydriatic responses were mediated mainly by efferent parasympathetic innervation, and, to a lesser extent, by sympathetic innervation. The alpha-adrenergic antagonist, phenoxybenzamine (0.3 mg/kg, intravenously (i.v.)), antagonized mydriasis of the neurally intact eye, but not that on the sympathectomized side. The alpha2-adrenergic antagonist, RS 79948 (0.3 mg/kg, i.v.), potentiated mydriasis of the normal eye, but was without either a potentiating or inhibitory effect on the mydriasis of the sympathectomized eye. In addition, the dopamine-receptor antagonist, haloperidol (1 mg/kg, i.v.), inhibited evoked mydriasis of the sympathectomized eye. These results suggest that, unlike some other species (cats and rats), alpha2-adrenoceptors do not mediate reflex mydriasis elicited by sciatic-nerve stimulation in the rabbit, and support the previous finding in humans that dopamine receptors may mediate this response.


Assuntos
Midríase/fisiopatologia , Receptores Adrenérgicos alfa 2/fisiologia , Reflexo Pupilar/fisiologia , Antagonistas de Receptores Adrenérgicos alfa 2 , Antagonistas Adrenérgicos alfa/administração & dosagem , Antagonistas Adrenérgicos alfa/farmacologia , Anestesia , Animais , Antagonistas de Dopamina/administração & dosagem , Antagonistas de Dopamina/farmacologia , Relação Dose-Resposta a Droga , Sinergismo Farmacológico , Estimulação Elétrica , Haloperidol/administração & dosagem , Haloperidol/farmacologia , Injeções Intravenosas , Isoquinolinas/administração & dosagem , Isoquinolinas/farmacologia , Masculino , Modelos Neurológicos , Midríase/induzido quimicamente , Naftiridinas/administração & dosagem , Naftiridinas/farmacologia , Fenoxibenzamina/administração & dosagem , Fenoxibenzamina/farmacologia , Coelhos , Receptores Dopaminérgicos/fisiologia , Reflexo Pupilar/efeitos dos fármacos , Nervo Isquiático/fisiologia , Nervo Isquiático/cirurgia , Simpatectomia
10.
J Ocul Pharmacol Ther ; 19(3): 255-63, 2003 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12828843

RESUMO

This study was undertaken to identify the alpha-adrenergic receptor type responsible for sympathetically evoked mydriasis in pentobarbital-anesthetized rabbits. Frequency-response curves of pupillary dilation were generated by stimulation of the preganglionic cervical sympathetic nerve (1-64 Hz). Evoked mydriatic responses were inhibited by systemic administration of nonselective alpha-adrenergic antagonists, phentolamine (0.3-10 mg/kg) and phenoxybenzamine (0.03-0.3 mg/kg), as well as the selective alpha(1)-adrenergic antagonist, prazosin (0.1-1 mg/kg). The alpha(2)-adrenergic antagonist, RS 79948 (0.3 mg/kg, i.v.) was without inhibitory effect, but potentiated the mydriatic response. In addition, the selective alpha(1A)-adrenoceptor antagonist, 5-methylurapidil (0.1-1 mg/kg, i.v.), antagonized the elicited mydriasis in a dose-dependent fashion. Unlike previous observations that prazosin does not block the adrenoceptor in rabbit iris dilator muscle, our results suggest that prazosin is effective in inhibiting neuronally elicited mydriasis in this species, and that alpha(1A)-adrenoceptors appear to mediate the response.


Assuntos
Antagonistas Adrenérgicos alfa/farmacologia , Midríase/fisiopatologia , Midriáticos/farmacologia , Pupila/efeitos dos fármacos , Sistema Nervoso Simpático/fisiopatologia , Antagonistas de Receptores Adrenérgicos alfa 1 , Antagonistas de Receptores Adrenérgicos alfa 2 , Animais , Relação Dose-Resposta a Droga , Estimulação Elétrica , Isoquinolinas/farmacologia , Masculino , Midríase/metabolismo , Naftiridinas/farmacologia , Fenoxibenzamina/farmacologia , Fentolamina/farmacologia , Piperazinas/farmacologia , Prazosina/farmacologia , Coelhos
11.
J Pharmacol Exp Ther ; 300(2): 521-5, 2002 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11805212

RESUMO

Evidence suggests that in some species (cats, rabbits, and possibly humans) alpha-adrenoceptors in the iris dilator muscle are "atypical" in that they cannot be readily classified by conventional criteria. This study was undertaken in an attempt to characterize the alpha-adrenoceptor subtype(s) mediating sympathetically elicited mydriasis in rats. Frequency-response pupillary dilator curves were generated by stimulation of the preganglionic cervical sympathetic nerve (1-32 Hz) in pentobarbital-anesthetized rats. Evoked responses were inhibited by systemic administration of nonselective alpha-adrenergic antagonists, phentolamine (0.3-10 mg/kg) and phenoxybenzamine (0.03-1 mg/kg). The selective alpha(1)-adrenergic antagonist, prazosin (0.01-1 mg/kg), also was effective, although alpha(2)-adrenergic antagonism with rauwolscine (0.1-1 mg/kg) was not. alpha(1A)-Adrenoceptor-selective antagonists, 2-([2,6-dimethoxyphenoxyethyl]aminomethyl)-1,4-benzodioxane (WB-4101; 0.1-1 mg/kg) and 5-methylurapidil (0.1-1 mg/kg), as well as the alpha(1D)-adrenoceptor-selective antagonist 8-[2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl]-8-azaspiro[4.5]decane-7,9-dione (BMY-7378; 1-3 mg/kg), were used to determine the subtype(s) involved. Evoked mydriasis was significantly antagonized by both WB-4101 and 5-methylurapidil but not by BMY-7378. These results suggest that, unlike some other species, adrenoceptors in the rat iris dilator mediating neurogenic mydriasis are "typical" and, in addition, can be characterized as being primarily of the alpha(1A)-adrenoceptor subtype.


Assuntos
Midriáticos/farmacologia , Pupila/efeitos dos fármacos , Receptores Adrenérgicos alfa 1/fisiologia , Sistema Nervoso Simpático/efeitos dos fármacos , Antagonistas Adrenérgicos alfa/farmacologia , Animais , Estimulação Elétrica , Injeções Intravenosas , Masculino , Midriáticos/antagonistas & inibidores , Ratos , Ratos Sprague-Dawley , Receptores Adrenérgicos alfa 1/efeitos dos fármacos
12.
Am J Rhinol ; 16(1): 49-55, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-11895194

RESUMO

The aim of this project was to develop and pharmacologically characterize an experimental dog model of nasal congestion in which nasal patency is measured using acoustic rhinometry. Solubilized compound 48/80 (0.3-3.0%) was administered intranasally to thiopental anesthetized beagle dogs to elicit nasal congestion via localized mast cell degranulation. Compound 48/80-induced effects on parameters of nasal patency were studied in vehicle-treated animals, as well as in the same animals pretreated 2 hours earlier with oral d-pseudoephedrine or chlorpheniramine. Local mast cell degranulation caused a close-related decrease in nasal cavity volume and minimal cross-sectional area (Amin) together with a highly variable increase in nasal secretions. Maximal responses were seen at 90-120 minutes after 48/80 administration. Oral administration of the adrenergic agonist, d-pseudoephedrine (3.0 mg/kg), significantly antagonized all of the nasal effects of compound 48/80 (3.0%). In contrast, oral administration of the histamine H1 receptor antagonist chlorpheniramine (10 mg/kg) appeared to reduce the increased nasal secretions but was without effect on the compound 48/ 80-induced nasal congestion (i.e., volume and Amin). These results show the effectiveness of using acoustic rhinometry in this anesthetized dog model. The observations that compound 48/80-induced nasal congestion was prevented by d-pseudoephedrine pretreatment, but not by chlorpheniramine, suggest that this noninvasive model system may provide an effective tool with which to study the actions of decongestant drugs in preclinical investigations.


Assuntos
Clorfeniramina/farmacologia , Efedrina/farmacologia , Obstrução Nasal/diagnóstico , Obstrução Nasal/tratamento farmacológico , Otolaringologia/métodos , Acústica , Resistência das Vias Respiratórias/efeitos dos fármacos , Animais , Modelos Animais de Doenças , Cães , Interações Medicamentosas , Masculino , Probabilidade , Valores de Referência , Sensibilidade e Especificidade
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