[Lipid peroxides and arteriosclerosis]. / Perekisi lipidov i ateroskleroz

The significance of free radical oxidation of phospholipids in tissues of animals with experimental atherosclerosis was investigated. By using modern physico-chemical methods an elevated content of polyunsaturated fatty acids and other lipids peroxides was discovered in the blood and the aorta of rabbits with experimental atheromatosis. The human blood demonstrated a low level of protective enzymatic systems and a high content of products secondary to peroxidal oxidation of the lipids. The mechanism accounting for the action of lipids peroxides on the vascular wall resulting in the formation of atheromatous plaques is considered.

[Lipid peroxides and atherosclerosis. The content of lipid peroxidation products in the blood in ischemic heart disease]. / Perekisi lipidov i ateroskleroz. Soderzhanie produktov perekisnogo okisleniia lipidov v krovi bol'nykh ishemicheskoi bolezni'iu serdtsa.

It was established that the content of primary (acylhydroperoxide) and secondary (intermollecular "seams" in aminophospholipids) products of lipid peroxide oxidation in blood of patients with ischemic heart disease is increased against the background of hyperlipidemia and hypercholesterolemia. It is suggested that intensification of lipid peroxide oxidation may play a role in the pathogenesis of atherosclerosis.

[Lipid peroxides and atherosclerosis. Free radical peroxidation of polyene lipids in the blood in ischemic heart disease]. / Perekisi lipidov i ateroskleroz, Svobodnoradikal'noe perekisnoe okislenie polienovykh lipidov v krovi bol'nykh ishemicheskoi bolezn'iu serdtsa.

Examination of blood in 114 males (35--60 years of age) with stage III of lipid hydroperoxides, acylhydroperoxides, intermol ecular cross-links in the aminophosphatides and secondary products of lipoperoxidation was increased considerably as compared to that in practically healthy males (30) of the same age. The activity of blood glutathione lipoperoxidase in the examined group of patients was sharply reduced.

[Oxidation state of membrane phospholipids and activity of the microsomal system of cholesterol hydroxylation in the liver of animals during atherogenesis]. / Stepen'okislennosti membrannykh fosfolipidov i aktivnost' mikrosomal'noi sistemy gidroksilirovaniia kholesterina v pecheni zhivotnykh pri aterogeneze.

Content of cholesterol in various tissues of experimental animals /blood plasma, thoracal department of aorta, liver microsomes/ was increased during atherogenesis depending on the level of aorta impairment. In atherogenesis content of both primary and secondary products of lipid peroxidation was also increased in microsomal membranes of rabbit and mini-pig liver tissue; the increase in the rate of microsomal lipid oxidation was accompanied by a decrease in the activity of membrane-bound microsomal 7 alpha-hydroxylase of cholesterol.

[Increase in the lipid peroxide content in the blood and aortas of rabbits with experimental atherosclerosis]. / Uvelichenie soderzhaniia perekisei lipidov v krovi i aortakh krolikov s éksperimental'nym aterosklerozom

Content of lipid peroxides was estimated in blood and aorta of intact rabbits by means of highly sensitive polarographic method, using a mercuric electrode. Content of lipoperoxides in blood and aorta was increased 4.4- and 3.6-fold, respectively, in animals with pronounced experimental atherosclerosis. The oxidation of lipoperoxides caused a distinct increase in content of intermolecular bindings in amino-containing phospholipids in aorta of rabbits with pronounced atheromatosis.

[Role of structural factor in the kinetics of free-radical oxidation of lipids in the membranes]. / O roli strukturnogo faktora v kinetike svobodnoradikal'nogo okisleniia lipidov v membranakh

Kinetics of free radical oxidation of lipids (formation of primary molecular products - hydroperoxides, secondary products - carbonyl substances and "fluorescent pigments") was studied in various membrane fragments, which were distinctly differentiated by their fatty acid composition. The non-enzymatic catalysis of lipoperoxidation in these systems was initiated by addition of Fe2+ + ascorbic acid. The membrane fragments were isolated from microsomes and mitochondria of rat liver tissue, external segments of frog retina rods, sarcoplasmic reticulum of rabbit and carp skeletal muscles and from microsomes of rabbit brain. In membrane structures the free radical oxidation of lipids developed following the same pattern as the liquid-phase oxidation of alkenes. But in phospholipids of biomembranes the rate of hydroperoxides formation did not correlate with the level of unsaturation of their polyene acyls. The rate of peroxidation of unsaturated fatty acids was distinctly determined by their structural organization in membranes.

[Role of lipid peroxides in the pathogenesis of arteriosclerosis. Detoxication of lipid peroxides by the glutathione-peroxidase system in the aorta]. / Rol' perekisei lipidov v patogenese ateroskleroza. Detoksikatsiia lipoperekisei glutation-peroksidaznoi sistemoi v aorte

In aorta of intact rabbits the high activity of glutathione-peroxidase, which detoxicates lipoperoxides, was observed. In aorta of animals with pronounced experimental atheromatosis the enzyme activity did not distinctly differ from the control values. The animals with high initial content of glutathione-peroxidase in aorta were shown to be less subjected to the impairment in alimentary atherosclerosis.

[Free radical peroxidation of liver mitochondrial and microsomal phospholipids in rat postnatal development]. / Svobodnoradikal'noe perekisnoe okislenie mitokhondrial'nykh i mikrosomal'nykh fosfolipidov pecheni v protsesse postnatal'nogo razvitiia krys.

Activities of both non-enzymic (ascorbate-dependent) and enzymic NADPH-dependent) peroxidation of microsomal and mitochondrial phospholipids are found to be increased in rat liver during postnatal development. It is suggested, that microsomal NADPH-dependent phospholipid dioxygenase forming a chemical modification of membrane polyenic acyls, can be a factor regulating the activities of membrane-linked enzymes under normal physiological processes.