RESUMO
Arterial elastin appears to be a proteinlipid complex with the lipid component being bound to elastin peptide groups. In atherosclerotic lesions the lipid content of elastin increases progressively with increasing severity of atherosclerosis. The increases in the lipid content of plaque elastin are mainly due to large increases in cholesterol with about 80% of the cholesterol being cholesterol ester. This deposition of cholesterol in elastin accounts for a substantial part of the total cholesterol accumulation in atherosclerotic lesions of all stages. The present in vitro study suggests that the mechanism involved in the deposition of lipids in arterial elastin may be an interaction of the elastin protein with serum or arterial low density or very low density lipoproteins (LDL and VLDL) resulting in a transfer of lipids, but not of lipoprotein protein to the elastin. No significant lipid transfer occurred from the high density lipoproteins or chylomicrons. The amount of lipid taken up by plaque elastin was strikingly higher than by normal elastin and consisted mainly of cholesterol with over 80% of the cholesterol being cholesterol ester. The precondition for the lipid accumulation in plaque elastin appeared to be an altered amino acid composition of the elastin protein consisting of an increase in polar amino acids and a reduction in cross-linking amino acids. Subsequent treatment of lipoprotein-incubated arterial elastin with hot alkali and apolipoproteins did not reverse the binding of lipoprotein lipid to diseased elastin.
Assuntos
Arteriosclerose/metabolismo , Elastina/metabolismo , Metabolismo dos Lipídeos , Lipoproteínas/metabolismo , Aminoácidos/análise , Colesterol/metabolismo , Quilomícrons/metabolismo , Elastina/análise , Humanos , Hidrólise , Hiperlipidemias/metabolismo , Técnicas In Vitro , Isótopos de Iodo , Lipoproteínas LDL/metabolismo , Lipoproteínas VLDL/metabolismo , Ligação Proteica , Hidróxido de Sódio/farmacologia , Tripsina/farmacologiaRESUMO
Elastin preparations from intimal layers and the media of normal and atherosclerotic human aortae were analyzed for protein and lipid content. In atherosclerotic aortae, elastin from plaques was compared with elastin from adjacent normal appearing areas of the same aorta. Arterial elastin purified by alkaline extraction appeared to be a protein-lipid complex containing free and ester cholesterol, phospholipids, and triglycerides. The lipid component of normal arterial elastin was small (1-2%). With increasing severity of atherosclerosis, there was a progressive accumulation of lipid in intimal elastin from plaques, reaching a mean lipid content of 37% in severe plaques. The increase in the lipid content of plaque elastic preparations was mainly due to large increases in cholesterol, over 80% of which was cholesteryl ester. This deposition of cholesterol in plaque elastin accounted for 20-34% of the total cholesterol content of the plaque. The increased lipid deposition in plaque elastin was associated with alterations in the amino acid composition of plaque elastin. In elastin from plaque intima, the following polar amino acids were increased significantly: aspartic acid, threonine, serine, glutamic acid, lysine, histidine, and arginine; whereas, cross-linking amino acids: desmosine, isodesmosine, and lysinonorleucine were decreased significantly. The amino acid and lipid composition of elastin from normal appearing aortic areas was comparable to that of normal arterial elastin except for intimal elastin directly adjacent to and medial elastin directly below the most severe plaques.The data indicate that the focal lipid deposition in early atherosclerotic plaques is due to a large extent to lipid accumulations in altered elastin protein of localized intimal areas. Continued lipid deposition in altered elastin appears to contribute substantially to the progressive lipid accumulation in the plaque. The study suggests that elastin of intimal elastic membranes may play an important role in the pathogenesis and progression of atherosclerosis.
Assuntos
Arteriosclerose/metabolismo , Elastina/análise , Lipídeos/análise , Proteínas/análise , Adolescente , Adulto , Idoso , Aorta/análise , Arginina/análise , Arteriosclerose/etiologia , Ácido Aspártico/análise , Colesterol/análise , Colesterol/metabolismo , Elastina/biossíntese , Ésteres/análise , Feminino , Glutamatos/análise , Histidina/análise , Humanos , Pulmão/análise , Lisina/análise , Masculino , Pessoa de Meia-Idade , Fosfolipídeos/análise , Serina/análise , Treonina/análise , Triglicerídeos/análise , TrítioRESUMO
Agents inhibiting calcium deposition into arteries are known to suppress atherosclerosis in animals. However, the precise role of calcium in atherogenesis is unknown. In this study, the specific Ca2+-antagonist lanthanum was used to attempt suppression of experimental atherosclerosis and to gain more insight into the possible effects of calcium on atherogenesis. Rabbits were fed an atherogenic diet with and without increasing doses of LaCl3. All cholesterol-fed rabbits showed marked increases in serum cholesterol and ca2+. Untreated atherogenic animals revealed pronounced gross and microscopic atherosclerosis and striking increases in the aortic content of cholesterol, collagen, "elastin," and calcium as well as of elastin calcium, polar amino acids, and cholesterol. With increasing LaCl3 dosage these abnormalities progressively decreased and were completely abolished at the highest dose. The ingested La3+ was absorbed only in small quantities and had no discernible effect on the calcium and connective tissue content of bone, skin, lung, heart, and skeletal muscle nor on myocardial function (left ventricle pressure and left ventricle dp/dt) or myocardial and muscle content in ATP and creatine phosphate. The data suggest that shifts in arterial Ca2+-distribution may play a decisive part in atherogenesis, and provision of arterial calcium homeostasis by La3+ a pivotal role in its prevention, despite hypercholesteremia. Other inhibitors of calcium deposition into arteries may exert their protective effect by similar mechanisms. However, a direct inhibition of atherogenesis by La3+ cannot entirely be ruled out in this study, although no direct effects of La3+ on tissue metabolism have as yet been reported.
Assuntos
Arteriosclerose/etiologia , Cálcio/fisiologia , Lantânio/farmacologia , Animais , Aorta/metabolismo , Aorta/patologia , Arteriosclerose/metabolismo , Arteriosclerose/patologia , Cálcio/antagonistas & inibidores , Cálcio/metabolismo , Dieta Aterogênica , Lantânio/metabolismo , Masculino , CoelhosRESUMO
Coarctation of the mid-thoracic aorata was surgically produced in mongrel dogs which were sacrificed from 4-12 wk after the operation. As compared to the findings in control animals, the sodium, chloride, and water content of the hypetensive portion of the coarcted thoracic aorta was significantly elevated, whereas the electrolyte and water content of the relatively normotensive portion of the coarcted aorta was normal. The sodium, potassium, and water content of the pulmonary artery, skeletal muscle, and cardiac muscle of the coarcted dog was not altered. These observations suggest that an elevated arterial pressure may influence the electrolyte and water composition of the arteries. The arterial pressure also may influence the content and synthesis of acid mucopolysaccharides (MPS) in the arteries since the content of sulfated MPS and the incorporation of injected radiosulfate into sulfated MPS were significantly increased in the hypertensive portion of the coarcted thoracic aorta but were significantly reduced in the relatively normotensive ("hypotensive") portion of the coarcted aorta. The observed increase in MPS may have been a factor directly responsible for the increase in the sodium content of the hypertensive aorta since MPS can act as polyelectrolytes and bind cations. Although the arterial pressure may influence certain metabolic functions in the arteries, it did not appear to have a direct effect on the arterial lipids since the lipid content of the hypertensive and of the relatively normotensive portions of the coarcted aorta were comparable to the values found in the normal aorta.
Assuntos
Aorta Torácica/análise , Coartação Aórtica/metabolismo , Lipídeos/análise , Artéria Pulmonar/análise , Ácidos Urônicos/análise , Equilíbrio Hidroeletrolítico , Animais , Determinação da Pressão Arterial , Artérias Carótidas/análise , Cães , Artéria Femoral/análise , Hipertensão/metabolismoRESUMO
Oxy-free radicals may be involved in the pathogenesis of accelerated atherosclerosis in hypertension. We evaluated the direct antioxidant potential of probucol in hypertensive arteries by studying the spatial immunohistochemical distribution of three primary antioxidant enzymes (AEs). Nineteen normocholesterolemic New Zealand White rabbits were divided into two groups: normotensive controls (NT; n = 6) and 13 animals rendered hypertensive by surgical coarctation of abdominal aorta. The hypertensive group was subdivided into hypertensive alone (HT; n = 8) and hypertensive treated with 1% probucol (PO) for 9 weeks (HT-P; n = 5). Blood pressure rose significantly in both hypertensive groups (P < .005). At autopsy, both hypertensive groups showed similarly significant increases in mean arterial intima-media thickness (IMT) whether or not they were treated with probucol. However, only HT rabbits revealed significant increases in the intima-media depth penetration of glutathione peroxidase, superoxide dismutase, and catalase AEs. By contrast, in HT-P animals probucol produced significant reductions of immunostaining of all three AEs compared to the HT group (P < .05). Additionally, specific macrophage immunostaining revealed that the arterial wall of HT rabbits had numerous (10 to 12 per high power field) subintimal and medial macrophages as compared to the HT-P animals (1 to 2 per high power field). The blood pressure level correlated significantly with IMT in all three groups, but with depth penetration of the three AEs only in the NT and HT groups. Probucol, therefore, appears to act in concert with the native arterial antioxidant enzymes as a potent free radical scavenger to reduce oxidative stress and thus attenuate the macrophage invasive response in hypertensive arteries.
Assuntos
Antioxidantes/farmacologia , Artérias/patologia , Hipertensão/patologia , Estresse Oxidativo/efeitos dos fármacos , Probucol/farmacologia , Animais , Antioxidantes/metabolismo , Artérias/fisiopatologia , Pressão Sanguínea/efeitos dos fármacos , Colesterol/sangue , Hipertensão/enzimologia , Hipertensão/fisiopatologia , Processamento de Imagem Assistida por Computador , Imuno-Histoquímica , Masculino , CoelhosAssuntos
Artérias/metabolismo , Arteriosclerose/prevenção & controle , Cálcio/metabolismo , Animais , Aorta/patologia , Arteriosclerose/metabolismo , Arteriosclerose/patologia , Cálcio/sangue , Colesterol/sangue , Dieta Aterogênica , Difosfonatos/uso terapêutico , Ácido Etidrônico/uso terapêutico , Lantânio/uso terapêutico , Macaca fascicularis , PamidronatoRESUMO
Treatment of atherosclerosis has mainly focused on decreasing low-density lipoprotein cholesterol (LDL-C). However, recent coronary angiographic trials revealed that aggressive lowering of LDL-C below 100 mg/dl arrests atherosclerosis progression in only 50-60% of patients. Furthermore, quantitative coronary angiography in these trials showed significant regression only in advanced fibrous-fatty plaques (> or = 50% stenosis) and not in the younger, more cell-proliferative lesions (< 50% stenosis). It is clear that lipid-lowering therapy has limited efficacy and there is therefore a need for other drugs, especially anti-proliferative agents, for secondary and primary prevention. To test this hypothesis, a new calcium antagonist, amlodipine, was studied for its anti-atherogenicity in non-human primates because of its known in vitro anti-cell proliferant, cell membrane stabilising and anti-oxidant properties. Amlodipine was found to normalise elevated plasma levels of oxidised LDL without reducing elevated total LDL-C levels in monkeys fed an atherogenic diet which, however, significantly suppressed atherosclerosis progression. These data suggest that amlodipine may be an excellent candidate, in combination with lipid-lowering drugs, for dual therapy of atherosclerotic vascular disease and may also be effective as monotherapy even when LDL-C is not lowered satisfactorily.
Assuntos
Anlodipino/uso terapêutico , Antioxidantes/uso terapêutico , Arteriosclerose/tratamento farmacológico , Lipoproteínas LDL/sangue , Animais , LDL-Colesterol/sangueRESUMO
Treatment of atherosclerosis has focused mainly on decreasing low-density lipoprotein cholesterol (LDL-C). However, recent coronary angiography trials have revealed that aggressive lowering of LDL-C below 100 mg/dl arrests atherosclerosis progression in only 50% to 60% of patients. Furthermore, with quantitative coronary angiography, significant regression occurred only in advanced fibrous-fatty plaques (> or = 50% stenosis) and not in the younger, more cell-proliferative lesions (< 50% stenosis). It is clear that lipid-lowering therapy has limited efficacy; therefore, other drugs, especially antiproliferative agents, may be useful for secondary and primary prevention. To test this hypothesis a new calcium antagonist, amlodipine, which has in vitro antiproliferative, cell membrane stabilizing, and antioxidative properties, was studied to determine whether it has antiatherogenic effects in nonhuman primates. Amlodipine normalized elevated levels of oxidized arterial cholesterol without reducing elevated total plasma cholesterol levels and significantly suppressed atherosclerosis progression in monkeys who had been fed an atherogenic diet. These data suggest that amlodipine may be an excellent candidate, in combination with lipid-lowering drugs, for dual therapy of atherosclerotic vascular disease, and also may be effective monotherapy, even when LDL-C is not lowered satisfactorily.
Assuntos
Anlodipino/uso terapêutico , Bloqueadores dos Canais de Cálcio/uso terapêutico , LDL-Colesterol/sangue , Doença da Artéria Coronariana/prevenção & controle , Hipolipemiantes/uso terapêutico , Animais , Doença da Artéria Coronariana/sangue , Doença da Artéria Coronariana/patologia , Quimioterapia Combinada , Haplorrinos , HumanosRESUMO
Two major features of atherosclerosis may be distinguished: (a) atherosis caused by lipid infiltration in cells and extracellularly and (b) sclerosis caused by connective tissue deposition and by functional disturbance of the endothelium, leading to impairment of endothelium-derived relaxing factor (EDRF)-release and reduced arterial compliance. Atherosis generally rarely causes clinical symptoms and, furthermore, is reversible by lowering of LDL-C. However, the clinically significant human lesion is the fibrous atheromatous plaque. Regression of established fibrous lesions by maximal lowering of LDL-C is slow in native coronary arteries and nonexistent in bypass grafts. However, there is good evidence from recent clinical trials that even in arteries with significant fibrous plaques, functional improvement of sclerosis (increased compliance) can be achieved by reducing maximally LDL-C in spite of remaining structural connective tissue abnormalities, presumably through normalization of EDRF. Atherosclerosis, therefore, appears to be best influenced by lipid lowering when it is in its atherosis phase. In the plaques of advanced atherosclerosis, when connective tissue sclerosis is present, agents working through mechanisms other than lipid lowering appear to be required for plaque reversal.
Assuntos
Arteriosclerose/patologia , Endotélio Vascular/patologia , Músculo Liso Vascular/patologia , Animais , Arteriosclerose/terapia , Doença da Artéria Coronariana/patologia , Doença da Artéria Coronariana/terapia , Seguimentos , HumanosRESUMO
When added to hydrochlorothiazide, enalapril is as effective as captopril in the treatment of moderate to severe hypertension. It is also effective when used as monotherapy, and may be incorporated into a regimen with diuretic and methyldopa or timolol for the treatment of more resistant patients. Enalapril has a good safety profile as measured by frequency of clinical and laboratory adverse experiences.
Assuntos
Enalapril/uso terapêutico , Hipertensão/tratamento farmacológico , Adulto , Idoso , Inibidores da Enzima Conversora de Angiotensina , Pressão Sanguínea/efeitos dos fármacos , Captopril/uso terapêutico , Ensaios Clínicos como Assunto , Relação Dose-Resposta a Droga , Método Duplo-Cego , Quimioterapia Combinada , Enalapril/administração & dosagem , Enalapril/efeitos adversos , Feminino , Humanos , Hidroclorotiazida/uso terapêutico , Hipertensão/enzimologia , Masculino , Metildopa/uso terapêutico , Pessoa de Meia-Idade , Distribuição Aleatória , Estatística como AssuntoAssuntos
Arteriosclerose/etiologia , Cálcio/antagonistas & inibidores , Animais , Arteriosclerose/tratamento farmacológico , Arteriosclerose/patologia , Arteriosclerose/fisiopatologia , Arteriosclerose/prevenção & controle , Cálcio/fisiologia , Colesterol/sangue , Colesterol/metabolismo , Colágeno/metabolismo , Dieta Aterogênica , Difosfonatos/uso terapêutico , Elastina/metabolismo , Ácido Etidrônico/uso terapêutico , Humanos , Lantânio/uso terapêutico , Macaca fascicularis , Masculino , Músculo Liso Vascular/patologia , Músculo Liso Vascular/fisiopatologia , Piperazinas/uso terapêutico , CoelhosAssuntos
Arteriosclerose/patologia , Tecido Conjuntivo/patologia , Animais , Artérias/metabolismo , Artérias/patologia , Arteriosclerose/metabolismo , Arteriosclerose/prevenção & controle , Cálcio/metabolismo , Cálcio/fisiologia , Colesterol na Dieta/farmacologia , Colágeno/metabolismo , Tecido Conjuntivo/efeitos dos fármacos , Tecido Conjuntivo/metabolismo , Elastina/metabolismo , Glicoproteínas/metabolismo , Glicosaminoglicanos/metabolismo , HumanosAssuntos
Artérias/patologia , Arteriosclerose/patologia , Elastina , Metabolismo dos Lipídeos , Proteínas , Aminoácidos , Aorta/patologia , Artérias/análise , Artérias/metabolismo , Arteriosclerose/metabolismo , Autorradiografia , Calcinose/patologia , Fenômenos Químicos , Química , Colesterol , Ensaios Clínicos como Assunto , Elastina/análise , Elastina/metabolismo , Humanos , Hidrólise , Técnicas In Vitro , Lipoproteínas/metabolismo , TrítioAssuntos
Aorta/imunologia , Arteriosclerose/imunologia , Proteínas do Sistema Complemento , Imunoglobulinas/metabolismo , Animais , Aorta/metabolismo , Sítios de Ligação de Anticorpos , Eletroforese das Proteínas Sanguíneas , Radioisótopos de Carbono , Colágeno , Imunofluorescência , Humanos , Soros Imunes , Imunodifusão , Imunoeletroforese , Imunoglobulina A/metabolismo , Imunoglobulina G/metabolismo , Imunoglobulina M/metabolismo , Leucina/metabolismo , Microscopia de Fluorescência , Coelhos/imunologiaRESUMO
Hypertensive stimuli in experimental animals appear to cause early arterial wall hypermetabolism before hypertension is established and before histomorphic changes are marked. Hypermetabolism and the large diffusion distances for oxygen characteristic of large arteries imply that mural hypoxia could occur early in the disease but this has not been studied. Therefore, we measured aortic wall tissue oxygen distributions in male New Zealand White rabbits 2 weeks (1) after production of upper body hypertension by coarctation of the abdominal aorta, (2) after sham operation, and (3) in normal controls. As compared to normal and sham operated animals, blood pressure in the coarctation animals was significantly elevated from control levels, and the oxygen partial pressures were significantly reduced throughout the media. However, the hypoxic mural oxygen tensions in this relatively thin wall were not low enough to terminate classical respiration. At the time of measurement, moderate microscopic pathology was already present, including increased connective tissue production. The accompanying histomorphic changes are compatible with reports of extrarespiratory oxygen sensitivity of connective tissue metabolism.
Assuntos
Artérias/metabolismo , Hipertensão/metabolismo , Oxigênio/metabolismo , Animais , Aorta/patologia , Artérias/patologia , Pressão Sanguínea , Dióxido de Carbono/sangue , Secções Congeladas , Concentração de Íons de Hidrogênio , Hipertensão/patologia , Microeletrodos , Oxigênio/sangue , Coelhos , Análise de RegressãoRESUMO
In 1904, Marchand recognized the consistent association of fatty degeneration and vessel stiffening and introduced the term "atherosclerosis" to indicate this combination. Current research is focused principally on the lipid component, but there is evidence that both aspects are reversible. Atheromatous lipids add significantly to the volume of lesions and thus contribute to vascular obstruction and end-organ damage. Reversal of atherosis has been observed in all the major species used in atherosclerosis research; rabbits, swine, dogs, chicks, pigeons, and subhuman primates. Direct evidence for reversal in humans is based on angiographic trials and is less extensive. One femoral artery and one coronary artery trial indicate that the lesions can be stabilized. CLAS, the largest angiographic trial to date, indicates that coronary lesion reversal is possible. Clinical effects of sclerosis are more subtle, and there is little evidence that sclerosis alone leads to end-organ damage. However, it should be noted that atherosclerotic lesions producing end-organ damage invariably have a major fibrous component. Sclerotic vessels have reduced systolic expansion and abnormally rapid pulse wave propagation, which can be measured noninvasively. Primate studies indicate that sclerosis is induced by hypercholesterolemic diets and is reversible when these diets are withdrawn. Changes in sclerosis may be another useful indicator of the formation and reversal of lesions and may involve changes in EDRF. Future studies of atherosclerosis reversal should use a combination of measures to evaluate both atherosis and sclerosis.
Assuntos
Arteriosclerose/fisiopatologia , Angiografia , Animais , Arteriosclerose/metabolismo , Arteriosclerose/terapia , Vasos Sanguíneos/patologia , Ensaios Clínicos como Assunto , Gorduras na Dieta/metabolismo , Ácidos Graxos/metabolismo , Humanos , Metabolismo dos Lipídeos , Indução de Remissão , EscleroseRESUMO
Thoracic aortae of normal rabbits were perfused with pancreatic elastase in vitro at 37 degrees C and 70 mm Hg pressure in the presence or absence of elastin ligands previously shown to stimulate or inhibit the enzymatic degradation of elastin. Perfusion with elastase results in an average of 3.6 lamellae degraded, whereas addition of sodium linoleate before and during the perfusion with elastase increases this value to 7.9 (P less than 0.001). Conversely, perfusion with the cationic detergent, dodecyltrimethylammonium chloride, completely prevents the degradation of elastic lamellae by elastase. These effects do not reflect alterations of the intrinsic catalytic activity of elastase, but apparently indicate the formation of complexes between the elastin ligands and arterial elastic lamellae, as is consistent with prior studies indicating such interactions between fatty acids or detergents and purified elastin. These studies suggest that agents such as fatty acids may significantly alter the metabolic susceptibility of elastin in vivo and possibly contribute to the degradation of elastic lamellae seen in arteries with advanced atherosclerosis.