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1.
Eur J Clin Microbiol Infect Dis ; 30(9): 1067-73, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21318732

RESUMO

The use of bacteriophages, instead of antibodies, in the ELISA-based detection of bacterial strains was tested. This procedure appeared to be efficient, and specific strains of Salmonella enterica and Escherichia coli could be detected. The sensitivity of the assay was about 10(5) bacterial cells/well (10(6)/ml), which is comparable with or outperforms other ELISA tests detecting intact bacterial cells without an enrichment step. The specificity of the assay depends on the kind of bacteriophage used. We conclude that the use of bacteriophages in the detection and identification of bacteria by an ELISA-based method can be an alternative to the use of specific antibodies. The advantages of the use of bacteriophages are their environmental abundance (and, thus, a possibility to isolate various phages with different specificities) and the availability of methods for obtaining large amounts of phage lysates, which are simple, rapid, cheap, and easy.


Assuntos
Técnicas Bacteriológicas/métodos , Infecções por Escherichia coli/diagnóstico , Escherichia coli/isolamento & purificação , Infecções por Salmonella/diagnóstico , Fagos de Salmonella/crescimento & desenvolvimento , Salmonella enterica/isolamento & purificação , Ensaio de Imunoadsorção Enzimática/métodos , Escherichia coli/virologia , Humanos , Salmonella enterica/virologia , Sensibilidade e Especificidade
2.
Biochemistry (Mosc) ; 76(7): 780-90, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21999539

RESUMO

The O-polysaccharide of Salmonella Telaviv was obtained by mild acid degradation of the lipopolysaccharide and studied by chemical methods (sugar and methylation analyses, Smith degradation, de-O-acetylation) and NMR spectroscopy. The structure of the O-polysaccharide was established. The repeating units that are proximal to the lipopolysaccharide core region mostly have a digalactose side chain and lack glucose, whereas those at the other end of the chain mostly do bear glucose but are devoid of the disaccharide side chain. This is the first structure established for the O-polysaccharide of a Salmonella serogroup O:28 (formerly M) strain characterized by subfactors O28(1) and O28(2). Knowledge of this structure and the structure of the O-polysaccharide of Salmonella Dakar (O28(1), O28(3)) established earlier is crucial for determination of the exact structures associated with subfactors O28(1), O28(2), and O28(3) and elucidation of the genetic basis of the close relationship between Escherichia coli O71 and S. enterica O:28 O-antigens.


Assuntos
Antígenos O/química , Salmonella enterica/química , Acetilglucosamina/análogos & derivados , Acetilglucosamina/análise , Sequência de Carboidratos , Dados de Sequência Molecular , Ressonância Magnética Nuclear Biomolecular , Salmonella enterica/imunologia
3.
FEMS Immunol Med Microbiol ; 21(4): 253-9, 1998 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-9752997

RESUMO

Lipopolysaccharide of Salmonella haarlem was hydrolyzed and the products separated. Native O-polysaccharide antigen was oxidised with sodium periodate followed by reduction with sodium borohydride. Native and chemically modified antigens were the subject of immunochemical studies. Monoclonal antibodies against S. haarlem and polyclonal rabbit antisera against S. haarlem, S. typhi and S. anatum bacteria were produced. The serological relationship between the lipopolysaccharide of Salmonella bacteria belonging to the two different groups D2 and E1 was demonstrated using haemagglutination reactions, inhibition of haemagglutination and immunoblotting. Cross-reactions were observed in haemagglutination reactions and in immunoblotting between antisera to S. haarlem, S. typhi and S. anatum. Factors 3,9,46 were found in the S. haarlem strain and the sugar composition for the epitopes of each factor was determined.


Assuntos
Epitopos de Linfócito B/imunologia , Antígenos O/imunologia , Salmonella/imunologia , Animais , Sequência de Carboidratos , Epitopos de Linfócito B/química , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Antígenos O/química , Coelhos
4.
FEMS Immunol Med Microbiol ; 21(4): 243-52, 1998 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-9752996

RESUMO

Lipopolysaccharide (LPS) of Salmonella haarlem was hydrolyzed and the products separated. The structure of the O-specific polysaccharide (OPS) was found from sugar and methylation analyses. Rhamnose, mannose, galactose and tyvelose were detected and their linkage modes were established. The structure was confirmed by 1H, homonuclear and heteronuclear correlations and 13C NMR spectra. Anomeric configurations were assigned by chromium trioxide oxidation and proton coupled 13C spectra. Sugar sequence was established from specific carbon shift data and nuclear Overhauser effect spectroscopy. The repeating unit structure of S. haarlem OPS as --> 3)-alpha-D-Galp-(1 --> 6)-[alpha-Tyvp-(1 --> 3)]-beta-D-Manp-(1 --> 4)-alpha-L-Rhap was estimated. No structural heterogeneity of the antigen was found.


Assuntos
Epitopos de Linfócito B/química , Antígenos O/química , Salmonella/química , Configuração de Carboidratos , Sequência de Carboidratos , Epitopos de Linfócito B/imunologia , Dados de Sequência Molecular , Antígenos O/imunologia , Salmonella/imunologia
5.
Int J Food Microbiol ; 21(1-2): 21-30, 1994 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-8155475

RESUMO

The epidemiological situation connected with S. enteritidis in Poland, in the years 1961-1991, is described. During this period there were two increases in infections and food poisonings, which were of an epidemiological character. The first epidemic, in 1962-1976, affected primarily small children and spread by contact in a hospital environment. It caused serious diseases, or dangerous complications in already existing illnesses, with high mortality. A few foci of later food poisonings were caused by infected meat or meat by-products. The second epidemic, which began in 1980 or 1981 and still exists, has already affected about 500,000 persons. It has often concerned cases of sporadic infections, mainly in the case of small children, but it has not been of a hospital epidemic character. It has been far more frequently associated with food poisoning outbreaks caused by contaminated ice-cream, cream cakes, eggs, mayonnaise and, less frequently, by meat and meat by-products. Attention is drawn to the large number of humans transmitting S. enteritidis infections in Poland.


Assuntos
Surtos de Doenças , Microbiologia de Alimentos , Intoxicação Alimentar por Salmonella/epidemiologia , Infecções por Salmonella/epidemiologia , Salmonella enteritidis , Adolescente , Adulto , Fatores Etários , Animais , Criança , Pré-Escolar , Ovos/microbiologia , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Carne/microbiologia , Polônia/epidemiologia , Salmonelose Animal/epidemiologia , Salmonella enteritidis/isolamento & purificação
6.
Bull Inst Marit Trop Med Gdynia ; 36(1-4): 103-15, 1985.
Artigo em Inglês | MEDLINE | ID: mdl-3841818

RESUMO

The activity of the Yersinia pestis phage on the plague envelope antigen has been described. The purified envelope antigen was shown to possess the receptor properties. The phage caused changes of viscosity and hemagglutination properties of the antigen. The phage-treated antigen showed the additional precipitation arc in the immunoelectrophoretic investigations. The adsorption of the Yersinia pestis phage on the sheep and human erythrocytes was examined. The human red cells of group B exhibited the highest receptor properties for the Yersinia pestis phage.


Assuntos
Bacteriófagos/metabolismo , Eritrócitos/microbiologia , Receptores Virais/metabolismo , Yersinia pestis/metabolismo , Adsorção , Animais , Antígenos de Bactérias/imunologia , Antígenos de Superfície/imunologia , Eritrócitos/metabolismo , Hemaglutinação , Humanos , Imunoeletroforese , Imunoeletroforese Bidimensional , Ovinos , Viscosidade , Yersinia pestis/imunologia
7.
Bull Inst Marit Trop Med Gdynia ; 44-45(1-4): 89-93, 1993.
Artigo em Inglês | MEDLINE | ID: mdl-7580356

RESUMO

As a result of fusion of in vitro immunized mouse splenocytes and myeloma Sp2/0 cells, stable hybridomas secreting monoclonal antibodies against Vi antigen were obtained. The monoclonal antibodies were of IgM class. Reactivity of monoclonal antibodies to Vi antigen of different origin was tested. The reactivity was found to be independent of O-acetylation degree of Vi antigen.


Assuntos
Anticorpos Monoclonais/isolamento & purificação , Antígenos de Bactérias/imunologia , Escherichia coli/imunologia , Imunoglobulina M/imunologia , Polissacarídeos Bacterianos , Salmonella typhi/imunologia , Animais , Anticorpos Monoclonais/classificação , Reações Antígeno-Anticorpo , Antígenos de Bactérias/química , Antígenos de Bactérias/isolamento & purificação , Citrobacter/imunologia , Hemaglutinação , Técnicas In Vitro , Camundongos , Camundongos Endogâmicos BALB C , Baço/imunologia , Células Tumorais Cultivadas
8.
Bull Inst Marit Trop Med Gdynia ; 41(1-4): 145-8, 1990.
Artigo em Inglês | MEDLINE | ID: mdl-2135918

RESUMO

The occurrence of S. enteritidis phage types in Poland during the years 1981-1990 was examined. The strains were isolated from man, animals, and from food-stuffs. The majority of strains belongs to the phage types 7 and 1. The comparison with the phage-types isolated in 1970-1975 in Poland is done. The stability of the phage types in reported outbreaks is considered.


Assuntos
Tipagem de Bacteriófagos , Intoxicação Alimentar por Salmonella/microbiologia , Salmonella enteritidis/classificação , Animais , Microbiologia de Alimentos , Humanos , Polônia , Fagos de Salmonella/isolamento & purificação
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