Sildenafil prevents right ventricular hypertrophy and improves heart rate variability in rats with pulmonary hypertension secondary to experimental diabetes.

Chronic treatment with sildenafil (SILD) is an effective protector on the development of cardiovascular complications of pulmonary hypertension (PH) and diabetes. However, to date, no studies have evaluated the effect of SILD on cardiopulmonary pathophysiology during PH secondary to type 1 diabetes. AIM: The present study aimed to evaluate the beneficial effects of chronic SILD treatment on pulmonary arterial pressure, right ventricular hypertrophy (RVH) and cardiac autonomic dysfunction in rats with PH secondary to diabetes. METODOLOGY: Male Sprague Dawley rats were randomly distributed into the control group (saline), diabetic group (60 mg/kg with streptozotocin), SILD-treated control group (20 mg/kg) and SILD-treated diabetic group. RESULTS: After 8 weeks the type 1 diabetic animals presented PH, endothelial dysfunction of the pulmonary arteries, electrocardiographic alterations, RVH and overexpression of phosphodiesterase type 5 in the heart. In type 1 diabetic animals, SILD treatment prevented the development of PH, endothelial dysfunction and RVH. SILD treatment also prevented alterations in the corrected QT period and heart rate variability and prevented overexpression of phosphodiesterase type 5. CONCLUSION: Our results indicate for the first time that SILD treatment prevents pulmonary arterial endothelial dysfunction, pulmonary hypertension, right ventricular hypertrophy and improves heart rate variability in type 1 diabetic rats.

Cardiovascular effects of isorhamnetin and quercetin in isolated rat and porcine vascular smooth muscle and isolated rat atria.

Isorhamnetin and quercetin produced endothelium-independent vasodilator effects in rat aorta, rat mesenteric arteries, rat portal vein and porcine coronary arteries. The effects of the two flavonoids were similar in arteries stimulated by noradrenaline, KCl, U46619 or phorbol esters but the two flavonoids were more potent in the coronary arteries than in the aorta. At high concentrations, they also induced a positive inotropic effect in isolated rat atria. Therefore, at least part of the in vivo effects of quercetin may result from its conversion to isorhamnetin which is the main metabolite of quercetin found in plasma. The arterial, venous and coronary vasodilator effects may contribute to the protective effects of flavonoids in ischaemic heart disease observed in epidemiological studies.

Respuesta de las arterias pulmonares de lechón al óxido nítrico y a dadores de óxido nítrico: Evolución con la edad postnatal y modulación por el estrés oxidativo / Nitric oxide- and nitric oxide donor-induced responses in piglet pulmonary arteries: Postnatal maturation and modulation by oxidative stress

En el presente estudio hemos analizado la influencia de la edad postnatal y los cambios en el nivel de estrés oxidativo sobre la vasodilatación pulmonar in vitro inducida por el NO endógeno, el NO exógeno y donadores de NO. Se han utilizado las arterias pulmonares procedentes de lechones de 1 día y de 2 semanas de edad para el registro de la fuerza contráctil. En las arterias pulmonares de lechones de 1 y 15 días de edad, el estrés oxidativo basal modula la acción vasodilatadora del NO, siendo la NAD(P)H oxidasa de la adventicia la principal fuente endógena del anión superóxido. La vasodilatación inducida por el NO de origen endotelial y por el NO exógeno aumenta con la edad, posiblemente por un incremento en la actividad de la ciclooxigenasa-1 en los primeros momentos de vida extrauterina que modularía el efecto vasodilatador del NO. Finalmente, encontramos que el NO y los donadores de NO, SNAP y nitroprusiato (SNP), difieren en la cinética y distribución regional de la liberación del NO, lo que influye en la susceptibilidad para la inactivación por el anión superóxido y por la oxihemoglobina (AU)