RESUMO
Five new (+)-oleuropeic acid derivatives, eucalmaidins A-E (1-5), together with 12 known compounds (6-17), were isolated from the fresh leaves of Eucalyptus maideni. Structures of the new compounds were determined on the basis of spectroscopic analyses (HSQC, HMBC, and (1)H-(1)H COSY), chemical degradation, and enzymatic hydrolysis. Of the tested compounds, only quercetin showed slight anti-herpes simplex virus 1 (HSV-1) activity in vitro.
Assuntos
Ácidos Cicloexanocarboxílicos/isolamento & purificação , Citotoxinas/isolamento & purificação , Medicamentos de Ervas Chinesas/isolamento & purificação , Eucalyptus/química , Plantas Medicinais/química , Animais , Chlorocebus aethiops , Ácidos Cicloexanocarboxílicos/química , Ácidos Cicloexanocarboxílicos/farmacologia , Citotoxinas/química , Citotoxinas/farmacologia , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Herpesvirus Humano 1/efeitos dos fármacos , Estrutura Molecular , Folhas de Planta/química , Quercetina/farmacologia , EstereoisomerismoRESUMO
To investigate the anti-herpesvirus mechanism of pentagalloylglucose (PGG), we compared the proteomic changes between herpes simplex virus type 1 (HSV-1) infected MRC-5 cells with or without PGG-treatment, and between non-infected MRC-5 cells with or without PGG-treatment by 2-DE and MS-based analysis. Differentially expressed cellular proteins were mainly involved with actin cytoskeleton regulation. Significantly, PGG can down-regulate cofilin1, a key regulator of actin cytoskeleton dynamics. PGG can inhibit HSV-1-induced rearrangements of actin cytoskeleton which is important for infectivity. Furthermore, cofilin1 knockdown by siRNA also inhibited the HSV-1-induced actin-skeleton rearrangements. Both PGG-treatment and cofilin1 knockdown can reduce HSV-1 DNA, mRNA, protein synthesis and virus yields. Altogether, the results suggested that down-regulating cofilin1 plays a role in PGG inhibiting HSV-1 infection. PGG may be a promising anti-herpesvirus agent for drug development.