RESUMO
BACKGROUND: Parasitic wasps constitute one of the largest group of venomous animals. Although some physiological effects of their venoms are well documented, relatively little is known at the molecular level on the protein composition of these secretions. To identify the majority of the venom proteins of the endoparasitoid wasp Chelonus inanitus (Hymenoptera: Braconidae), we have randomly sequenced 2111 expressed sequence tags (ESTs) from a cDNA library of venom gland. In parallel, proteins from pure venom were separated by gel electrophoresis and individually submitted to a nano-LC-MS/MS analysis allowing comparison of peptides and ESTs sequences. RESULTS: About 60% of sequenced ESTs encoded proteins whose presence in venom was attested by mass spectrometry. Most of the remaining ESTs corresponded to gene products likely involved in the transcriptional and translational machinery of venom gland cells. In addition, a small number of transcripts were found to encode proteins that share sequence similarity with well-known venom constituents of social hymenopteran species, such as hyaluronidase-like proteins and an Allergen-5 protein.An overall number of 29 venom proteins could be identified through the combination of ESTs sequencing and proteomic analyses. The most highly redundant set of ESTs encoded a protein that shared sequence similarity with a venom protein of unknown function potentially specific of the Chelonus lineage. Venom components specific to C. inanitus included a C-type lectin domain containing protein, a chemosensory protein-like protein, a protein related to yellow-e3 and ten new proteins which shared no significant sequence similarity with known sequences. In addition, several venom proteins potentially able to interact with chitin were also identified including a chitinase, an imaginal disc growth factor-like protein and two putative mucin-like peritrophins. CONCLUSIONS: The use of the combined approaches has allowed to discriminate between cellular and truly venom proteins. The venom of C. inanitus appears as a mixture of conserved venom components and of potentially lineage-specific proteins. These new molecular data enrich our knowledge on parasitoid venoms and more generally, might contribute to a better understanding of the evolution and functional diversity of venom proteins within Hymenoptera.
Assuntos
Etiquetas de Sequências Expressas , Parasitos/genética , Parasitos/metabolismo , Proteômica/métodos , Venenos de Vespas/metabolismo , Vespas/genética , Vespas/metabolismo , Sequência de Aminoácidos , Animais , Teorema de Bayes , Bases de Dados Genéticas , Eletroforese em Gel de Poliacrilamida , Biblioteca Gênica , Proteínas de Insetos/química , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , Filogenia , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Venenos de Vespas/química , Venenos de Vespas/genética , Vespas/enzimologiaRESUMO
Polydnaviruses (PDVs) are unique symbiotic viruses associated with parasitic wasps; they replicate only in the calyx cells of a wasp's ovaries and are transferred at oviposition along with the parasitoid egg into the lepidopteran host. The DNA packaged in the viral particles encodes factors that manipulate the host's immune defences and development to benefit the parasitoid. PDVs are found in two subfamilies of ichneumonids (ichnoviruses) and in braconids of the microgastroid complex (bracoviruses). We recently showed that the latter derive from an ancestral nudivirus, as 24 nudivirus-related genes were identified in ovaries of two distantly related braconids at the stage of virion formation. Here, we present a comprehensive analysis of the viral particle proteins of the Chelonus inanitus bracovirus (CiBV). Proteins of purified CiBV particles were analysed by mass spectrometry and amino acid sequences matched to the existing ovarian-cDNA database. In addition, transcript quantities of identified genes were measured by quantitative real-time PCR in female pupae at the onset and peak of virion formation and at corresponding stages in male pupae. This combined approach allowed the identification of 44 CiBV particle proteins: 16 were nudivirus-related, three had similarity to ovarian proteins of another braconid, 11 had similarity to cellular proteins and 14 had no similarity to known proteins. The transcripts of all of them increased in female, but not male, pupae. These data confirm the important contribution of nudivirus genes but also indicate the presence of many lineage- or species-specific proteins possibly involved in the parasitoid-host interaction.
Assuntos
Himenópteros/virologia , Polydnaviridae/química , Proteínas Virais/análise , Vírion/química , Animais , Perfilação da Expressão Gênica , Biblioteca Gênica , Genes Virais , Espectrometria de Massas , Polydnaviridae/isolamento & purificação , Pupa/virologia , Proteínas Virais/genética , Vírion/isolamento & purificaçãoRESUMO
Very few obligatory relationships involve viruses to the remarkable exception of polydnaviruses (PDVs) associated with tens of thousands species of parasitic wasps that develop within the body of lepidopteran larvae. PDV particles, injected along with parasite eggs into the host body, act by manipulating host immune defences, development and physiology, thereby enabling wasp larvae to survive in a potentially harmful environment. Particle production does not occur in infected tissues of parasitized caterpillars, but is restricted to specialized cells of the wasp ovaries. Moreover, the genome enclosed in the particles encodes almost no viral structural protein, but mostly factors used to manipulate the physiology of the parasitized host. We recently unravelled the viral nature of PDVs associated with braconid wasps by characterizing a large set of nudivirus genes residing permanently in the wasp chromosome(s). Many of these genes encode structural components of the bracovirus particles and their expression pattern correlates with particle production. They constitute a viral machinery comprising a large number of core genes shared by nudiviruses and baculoviruses. Thus bracoviruses do not appear to be nudiviruses remnants, but instead complex nudiviral devices carrying DNA for the delivery of virulence genes into lepidopteran hosts. This highlights the fact that viruses should no longer be exclusively considered obligatory parasites, and that in certain cases they are obligatory symbionts.
Assuntos
Polydnaviridae/genética , Vírion/genética , Vespas/virologia , Sequência de Aminoácidos , Animais , Sequência Conservada , DNA Viral , Feminino , Genoma Viral , Dados de Sequência Molecular , Nucleocapsídeo/genética , Nucleocapsídeo/fisiologia , Ovário/metabolismo , Ovário/virologia , Polydnaviridae/fisiologia , Polydnaviridae/ultraestrutura , Alinhamento de Sequência , Transcrição Gênica , Proteínas do Envelope Viral/genética , Vírion/fisiologiaRESUMO
Larvae of endoparasitoids undergo extensive morphological changes and often have special features to allow their development inside the host. We present the first detailed study on the development of the anal vesicle and the gut. The analyses reveal that the anal vesicle is first seen on the dorsal side of the abdomen as an internal structure covered by a membrane. The morphology of the abdomen then changes intensively: new segments are formed and the anal vesicle develops from a crest of large cells to a protrusion. Towards the end of the first instar, the anal vesicle is fully evaginated and no longer covered by a membrane; the large epithelial cells have microvilli on their apical side which suggests uptake of nutrients from the host's haemolymph. When the larva has moulted to the second instar, the ultrastructure of the anal vesicle begins to change and shows signs of degeneration. In this stage the epithelium of the midgut is fully developed and has a brush border which suggests that nutrient uptake occurs now primarily through the midgut. The anal vesicle then degenerates completely. The salivary glands are prominent already in first instar larvae and appear to produce and release a host regulatory 212 kD protein.
Assuntos
Canal Anal/fisiologia , Proteínas de Insetos/fisiologia , Metamorfose Biológica/fisiologia , Glândulas Salivares/fisiologia , Spodoptera/parasitologia , Vespas/fisiologia , Canal Anal/crescimento & desenvolvimento , Canal Anal/ultraestrutura , Animais , Western Blotting , Interações Hospedeiro-Parasita , Imuno-Histoquímica , Larva/crescimento & desenvolvimento , Larva/fisiologia , Larva/ultraestrutura , Microscopia Confocal , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Glândulas Salivares/crescimento & desenvolvimento , Glândulas Salivares/ultraestrutura , Vespas/crescimento & desenvolvimento , Vespas/ultraestruturaRESUMO
Chelonus inanitus is a solitary egg-larval endoparasitoid, which feeds on host haemolymph during its internal phase. Parasitization induces in the host Spodoptera littoralis a precocious onset of metamorphosis and a developmental arrest in the prepupal stage. At this stage the parasitoid larva emerges from the host and consumes it. We show here that parasitization and the co-injected polydnaviruses affect the nutritional physiology of the host mainly in the last larval instar. Polydnaviruses cause a reduced uptake of food and an increase in the concentration of free sugars in the haemolymph and of glycogen in whole body. The parasitoid larva, along with polydnaviruses, causes a reduction of proteins in the host's plasma and an accumulation of lipids in whole body. Dilution of host haemolymph led to a reduced concentration of lipid in parasitoid larvae and a reduced survival rate. Thus, a sufficient concentration of nutrients in the host's haemolymph appears to be crucial for successful parasitoid development. Altogether, the data show that the parasitoid and the polydnavirus differentially influence host nutritional physiology and that the accumulated lipids and glycogen are taken up by the parasitoid in its haematophagous stage as well as through the subsequent external host feeding.
Assuntos
Fenômenos Fisiológicos da Nutrição Animal , Hemolinfa/química , Polydnaviridae , Spodoptera/parasitologia , Vespas/crescimento & desenvolvimento , Vespas/virologia , Animais , Proteínas Sanguíneas , Ingestão de Alimentos/fisiologia , Eletroforese em Gel Bidimensional , Glicogênio/metabolismo , Interações Hospedeiro-Parasita/fisiologia , Larva/parasitologia , Larva/fisiologia , Lipídeos/análise , Spodoptera/fisiologiaRESUMO
The egg-larval parasitoid Chelonus inanitus induces in its host Spodoptera littoralis two major developmental effects, namely a precocious onset of metamorphosis followed by a developmental arrest in the prepupal stage. Along with each egg, the wasp injects polydnavirus and venom into the host egg. The polydnavirus has been shown to play a major role in inducing the developmental arrest while the parasitoid larva is instrumental in inducing the precocious onset of metamorphosis. Here we report that experimental dilution of haemolymph of polydnavirus-containing larvae can partially prevent the developmental arrest while injection of native, but not of heat-treated, haemolymph or plasma from polydnavirus-containing larvae into nonparasitized larvae could induce developmental arrest in 14-15% of the larvae. This illustrates that heat-labile factors present in haemolymph play a role in causing developmental arrest. Injection of parasitoid medium increased the proportion of larvae entering metamorphosis precociously while injection of antibodies against a parasitoid-released protein had the opposite effect; this indicates that this protein and possibly other parasitoid-released substances are involved in inducing the precocious onset of metamorphosis. Analysis of the plasma proteome of nonparasitized, parasitized and polydnavirus-containing larvae revealed that the developmental effects are associated with only minor differences: eleven low abundant viral or virus-induced proteins and five parasitoid-released proteins were seen at specific stages of the host.
Assuntos
Perfilação da Expressão Gênica , Hemolinfa/metabolismo , Proteínas de Insetos/metabolismo , Polydnaviridae/fisiologia , Spodoptera/parasitologia , Spodoptera/virologia , Animais , Anticorpos Antivirais/metabolismo , Regulação para Baixo , Hemolinfa/efeitos da radiação , Interações Hospedeiro-Parasita , Metamorfose Biológica/fisiologia , Spodoptera/metabolismo , Spodoptera/efeitos da radiação , Raios XRESUMO
Chelonus inanitus (Braconidae) is a solitary egg-larval parasitoid which lays its eggs into eggs of Spodoptera littoralis (Noctuidae); the parasitoid larva then develops in the haemocoel of the host larva. Host embryonic development lasts approx. 3.5 days while parasitoid embryonic development lasts approx. 16 h. All stages of host eggs can be successfully parasitized, and we show here that either the parasitoid larva or the wasp assures that the larva eventually is located in the host's haemocoel. (1) When freshly laid eggs, up to almost 1-day-old, are parasitized, the parasitoid hatches while still in the yolk and enters the host either after waiting or immediately through the dorsal opening. (2) When 1-2-day-old eggs are parasitized, the host embryo has accomplished final dorsal closure and is covered by an embryonic cuticle when the parasitoid hatches; in this case the parasitoid larva bores with its moving abdominal tip into the host. (3) When 2.5-3.5-day-old eggs are parasitized, the wasp oviposits directly into the haemocoel of the host embryo; from day 2 to 2.5 the embryo is still very small and the wasps, after probing, often restrain from oviposition for a few hours.
Assuntos
Oviposição/fisiologia , Spodoptera/parasitologia , Vespas/fisiologia , Animais , Interações Hospedeiro-Parasita , Larva/parasitologia , Larva/fisiologia , Larva/ultraestrutura , Microscopia Eletrônica de Varredura , Óvulo/citologia , Óvulo/parasitologia , Spodoptera/embriologia , Spodoptera/ultraestrutura , Vespas/embriologia , Vespas/ultraestruturaRESUMO
In situ hybridizations show that 5 min after parasitization, polydnavirus DNA is in close vicinity of the parasitoid egg, but 5 h later also in the yolk and partially in the host embryo. Fifteen hours after parasitization, the viral DNA is seen all over the host embryo and hardly in the yolk. The tissue distribution of the viral DNA was analysed and quantified by dot blots in the fifth instar parasitized larvae. On a per host basis, haemocytes and fat body contained the highest amount of viral DNA, while nervous tissue, intestinal tract and carcass contained less. Of the three viral segments tested, all were found in all tissues. Relative to the quantity of host DNA, viral DNA was most abundant in haemocytes, about five times less abundant in fat body and nervous tissue and about 25 times less abundant in intestinal tract. The total quantity of viral DNA per host was 444+/-145 pg which is similar to the quantity injected by the wasp; thus, the viral DNA persists throughout parasitization. The parasitoid larva contains 820+/-80 pg viral DNA integrated in the genome. This illustrates that the dose of viral DNA injected in virions represents approximately one third of the total viral genomic information present in a host at a late stage of parasitism.
Assuntos
DNA Viral/genética , Himenópteros/virologia , Larva/virologia , Óvulo/virologia , Spodoptera/virologia , Animais , Sequência de Bases , Primers do DNA , Hibridização In SituRESUMO
Chelonus inanitus (Braconidae) is a solitary egg-larval parasitoid of Spodoptera littoralis (Noctuidae). Along with the egg it also injects polydnaviruses (CiV) and venom, which are prerequisites for successful parasitoid development. CiV protects the parasitoid from encapsulation by the host's immune system and induces a developmental arrest in the prepupal stage. The polydnavirus genome consists of several double-stranded circular DNA segments. Proviral DNA is integrated in the wasp's genome and virus replication is restricted to the wasp's ovary. Here, the analysis of eight CiV genes located on five different segments revealed four patterns of expression in the course of parasitization: early, late, persistent but variable, and early and late. The comparison between parasitized and CiV/venom only containing hosts indicated that the presence of the parasitoid larva modulates transcript levels. Haemocytes, fat body and nervous tissue contained viral transcripts, values being highest in haemocytes. Small amounts of CiV transcripts were also observed in parasitoid larvae and pupae, suggesting transcription from the proviral integrated form of viral DNA. This is the first comparative analysis of the expression patterns of several viral genes in both parasitized and CiV/venom only containing hosts over the entire period of parasitization, and it reveals intricate interactions between the parasitoid, the polydnavirus and the host.
Assuntos
Himenópteros/fisiologia , Polydnaviridae/genética , Spodoptera/parasitologia , Animais , DNA Viral/genética , DNA Viral/isolamento & purificação , Regulação Viral da Expressão Gênica , Interações Hospedeiro-Parasita , Himenópteros/crescimento & desenvolvimento , Himenópteros/virologia , Larva , Óvulo , Reação em Cadeia da Polimerase , Pupa , RNA Viral/isolamento & purificação , Transcrição GênicaRESUMO
The egg-larval parasitoid Chelonus inanitus injects bracoviruses (BVs) and venom along with the egg into the host egg; both components are essential for successful parasitoid development. All stages of eggs of its natural host, Spodoptera littoralis, can be successfully parasitized, i.e. from mainly a yolk sphere to a fully developed embryo. Here, we show that the venom contains at least 25 proteins with masses from 14kDa to over 300kDa ranging from acidic to basic. The majority is glycosylated and their persistence in the host is short when old eggs are parasitized and much longer when young eggs are parasitized. Physiological experiments indicated three different functions. (1) Venom synergized the effect of BVs in disrupting host development when injected into third instar larvae. (2) Venom had a transient paralytic effect when injected into sixth instar larvae. (3) In vitro experiments with haemocytes of fourth instar larvae suggested that venom alters cell membrane permeability. We propose that venom promotes entry of BVs into host cells and facilitates placement of the egg in the embryo's haemocoel when old eggs are parasitized. The multifunctionality of the venom might thus be essential in enabling parasitization of all stages of host eggs.
Assuntos
Spodoptera/parasitologia , Venenos de Vespas/química , Vespas/química , Animais , Interações Hospedeiro-Parasita , Proteínas de Insetos/química , Larva/crescimento & desenvolvimento , Larva/metabolismo , Larva/parasitologia , Larva/virologia , Peso Molecular , Óvulo/crescimento & desenvolvimento , Óvulo/metabolismo , Óvulo/parasitologia , Óvulo/virologia , Polydnaviridae/fisiologia , Spodoptera/crescimento & desenvolvimento , Spodoptera/metabolismo , Spodoptera/virologia , Venenos de Vespas/metabolismo , Vespas/metabolismoRESUMO
In the solitary egg-larval parasitoid Chelonus inanitus (Braconidae) both polydnavirus and the parasitoid larva manipulate host development. Parasitization leads to a premature drop in juvenile hormone titre and a precocious onset of metamorphosis in the 5th larval instar. The C. inanitus bracovirus (CiBV) alone causes a reduction in host ecdysteroid titres at the pupal cell formation stage and prevents pupation. Here we report three new findings. (1) We show that parasitization causes a reduction in haemolymph ecdysteroid titre immediately after the moult to the 5th instar; similarly low values were seen in nonparasitized larvae after the moult to the 6th instar. These data along with parasitoid removal experiments indicate that the low ecdysteroid titre after the moult is a very early sign of the upcoming metamorphosis. (2) In vitro experiments with prothoracic glands and brain extracts showed that CiBV affects both prothoracic glands and prothoracicotropic hormone after the stage of pupal cell formation. (3) In the haemolymph of parasitized larvae the ecdysteroid titre increased in the late cell formation stage, i.e. immediately before egression of the parasitoid. In vitro experiments showed that late 2nd instar parasitoids release ecdysteroids and are thus very likely responsible for the rise in host ecdysteroids.
Assuntos
Ecdisteroides/metabolismo , Interações Hospedeiro-Parasita , Polydnaviridae/fisiologia , Spodoptera/parasitologia , Spodoptera/virologia , Vespas/crescimento & desenvolvimento , Animais , Hemolinfa/metabolismo , Larva/crescimento & desenvolvimento , Larva/fisiologia , Estágios do Ciclo de Vida , Muda , Spodoptera/crescimento & desenvolvimento , Spodoptera/metabolismo , Vespas/fisiologiaRESUMO
Many species of parasitoid wasps inject polydnavirus particles in order to manipulate host defenses and development. Because the DNA packaged in these particles encodes almost no viral structural proteins, their relation to viruses has been debated. Characterization of complementary DNAs derived from braconid wasp ovaries identified genes encoding subunits of a viral RNA polymerase and structural components of polydnavirus particles related most closely to those of nudiviruses--a sister group of baculoviruses. The conservation of this viral machinery in different braconid wasp lineages sharing polydnaviruses suggests that parasitoid wasps incorporated a nudivirus-related genome into their own genetic material. We found that the nudiviral genes themselves are no longer packaged but are actively transcribed and produce particles used to deliver genes essential for successful parasitism in lepidopteran hosts.
Assuntos
DNA Viral , Polydnaviridae/genética , Vespas/virologia , Sequência de Aminoácidos , Animais , Baculoviridae/genética , Evolução Biológica , DNA Viral/análise , Etiquetas de Sequências Expressas , Feminino , Genoma de Inseto , Dados de Sequência Molecular , Ovário/virologia , Polydnaviridae/fisiologia , Proteínas Estruturais Virais/genética , Vírion/genética , Integração ViralRESUMO
Polydnaviruses are only found in symbiotic association with parasitic wasps within the families Ichneumonidae and Braconidae (ichnoviruses and bracoviruses). They have a segmented genome consisting of circular double-stranded DNA. In the proviral linear form they are integrated in the wasp's genome; in two bracoviruses, segments were found to be clustered. Proviral segments have direct terminal repeats. Segment excision has been proposed to occur through juxtaposition of these repeats by formation of a loop and recombination; one copy of the repeat then ends up in the circular segment and one in the rejoined DNA. Here we analysed the excision/circularization site of four segments of the Chelonus inanitus bracovirus (CiV) and found that they are similar to the two already known sites; on the basis of the combined data an extended excision site motif was found. Analyses of segment flanking sequences led to the first identification of one complete and several partial spacers between proviral segments in a polydnavirus. The spacer between the proviral segments CiV14 and CiV22.5 has a length of 2065 bp; the terminal repeats of CiV14 and CiV22.5 were seen to have an opposite orientation and from this a model on the spacial organization of the loops of the proviral cluster is proposed. Through various approaches it was shown that spacers are not excised or injected into the host. Measurement of relative abundances of various segments in proviral and excised form indicates for the first time that abundant segments are present in multiple copies in the proviral form.
Assuntos
Genoma Viral , Polydnaviridae/genética , Provírus/genética , Vespas/virologia , Animais , Sequência de Bases , DNA Viral/genética , Feminino , Dados de Sequência Molecular , Polydnaviridae/química , Provírus/químicaRESUMO
Polydnaviruses are symbiotic viruses of endoparasitic wasps, which are formed in their ovary and injected along with the eggs into the host. They manipulate the host in a way to allow successful parasitoid development. A hallmark of polydnaviruses is their segmented genome consisting of several circles of double-stranded DNA. We are studying the solitary egg-larval parasitoid Chelonus inanitus (Braconidae) parasitizing Spodoptera littoralis (Noctuidae). The polydnavirus of Chelonus inanitus (CiV) protects the parasitoid larva from encapsulation by the host's immune system, slightly modifies host nutritional physiology, and induces a developmental arrest of the host in the prepupal stage. Here we present data on newly identified CiV genes and their expression patterns in the course of parasitization. None of these genes has similarity to other genes and so far no gene families could be found. A rough estimation of transcript quantities revealed that even the most highly expressed CiV genes reach maximal values, which are 250 times lower than actin. This indicates that the CiV-induced alterations of the host are brought about by a concerted action of low levels of transcripts. In an overview, we show the expression patterns of all CiV genes analysed up to now; they indicate that several genes with similar expression patterns (early, persistent, intermediate, or late) are grouped together on the same segment. This is the first observation of this type. It suggests that one function of the segmentation of the polydnavirus genome may be the grouping together of genes, which are regulated in a similar manner.
Assuntos
Regulação Viral da Expressão Gênica , Himenópteros/metabolismo , Himenópteros/virologia , Polydnaviridae/genética , Transcrição Gênica , Proteínas Virais/genética , Animais , Antioxidantes/metabolismo , Corpo Adiposo , Perfilação da Expressão Gênica , Hemolinfa , Larva/virologia , Longevidade , Malondialdeído/metabolismo , Especificidade de Órgãos , Óvulo/virologiaRESUMO
Successful parasitism of some endoparasitic wasps depends on an obligately symbiotic association with polydnaviruses. These unique viruses have a segmented genome consisting of circles of double-stranded (ds) DNA and do not replicate in the parasitized host. They are produced in the wasp's ovary and injected into the host along with the egg. Chelonus inanitus is an egg-larval parasitoid; its polydnavirus (CiV) has been shown to protect the parasitoid larva from the host's immune system and to induce developmental arrest in the prepupal stage. The genome of CiV consists of at least 10-12 segments and five have been sequenced up to now. Here, the complete (CiV12g2) or partial (CiV12g1, CiV16.8g1) cloning of three new CiV genes is reported. All three occur only on one viral segment and have no similarity to other known polydnavirus genes, with the exception of a high similarity of CiV12g1 to CiV14g1 and CiV12g2 to CiV14g2. Furthermore, the first attempt of in vivo application of RNA interference to study the function of polydnavirus genes is shown. Injection of dsRNA of two late- and one early- and late-expressed CiV genes into CiV/venom-containing host eggs partially rescued last-instar larvae from developmental arrest. Injection of the same dsRNAs into parasitized eggs partially reduced parasitoid survival, mainly by preventing the successful emergence of the parasitoid from the host. These viral genes thus seem to be involved in inducing developmental arrest and in keeping the cuticle soft, which appears to be necessary for parasitoid emergence and host feeding.
Assuntos
Clonagem Molecular , Regulação Viral da Expressão Gênica , Polydnaviridae/genética , Interferência de RNA , Proteínas Virais/metabolismo , Vespas/virologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Genes Virais , Interações Hospedeiro-Parasita , Larva/virologia , Dados de Sequência Molecular , Óvulo/virologia , Polydnaviridae/metabolismo , Pupa/virologia , Spodoptera/crescimento & desenvolvimento , Spodoptera/virologia , Proteínas Virais/genética , Vespas/crescimento & desenvolvimentoRESUMO
Polydnaviruses are unique symbiotic viruses that are formed only in calyx cells in the ovary of parasitic wasps in the families Braconidae and Ichneumonidae; accordingly, two genera, Bracovirus and Ichnovirus are recognized. We have presented a detailed ultrastructural analysis of ovary and calyx cell differentiation and virion morphogenesis, together with the first data on virion release in a bracovirus. Differentiation of the ovary into germarium/vitellarium and the calyx region begins immediately after pupation. In the periphery and central part of the calyx, some cells and their nuclei begin to enlarge and the DNA content increases. The calyx cell nuclei then further increase and become highly lobulated, nuclear pores become very abundant and the cytoplasm is rich in ribosomes. This suggests synthesis and import of viral envelope proteins as viral envelopes appear in the nuclei shortly later. The appearance of viral envelopes is accompanied by a swelling of the nucleus and a change in electron density. Thereafter, the calyx cells reach the final stage with a highly swollen nucleus containing virogenic stroma and mature virions with nucleocapsids. Up to this stage, the DNA content of nuclei increases 120-fold and the volume 45-fold. The mature calyx cells are positioned in the vicinity of the oviduct lumen; for release of virions first the nuclear and then the plasma membrane disintegrate. On the border of the oviduct lumen, cells of an epithelial layer become phagocytic and remove debris, leading to a calyx fluid that contains only densely packed virions.
Assuntos
Ovário/citologia , Ovário/crescimento & desenvolvimento , Polydnaviridae/fisiologia , Vírion/metabolismo , Vespas/virologia , Animais , Diferenciação Celular , DNA/metabolismo , DNA Viral/metabolismo , Feminino , Morfogênese , Ovário/ultraestrutura , Ovário/virologia , Polydnaviridae/genética , Simbiose , Vírion/fisiologia , Replicação Viral , Vespas/crescimento & desenvolvimentoRESUMO
Chelonus inanitus (Braconidae, Hymenoptera) is a solitary egg-larval parasitoid of Spodoptera littoralis. Along with the egg the female wasp injects polydnaviruses, which are prerequisites for successful parasitoid development. The polydnavirus genome is segmented and consists of double-stranded circular DNA. Proviral DNA is integrated in the wasp's genome; virus replication is restricted to the wasp's ovary and does not occur in the parasitized host. The polydnavirus of C. inanitus (CiV) protects the parasitoid larva from encapsulation by the host's immune system and causes a developmental arrest of the host in the prepupal stage. Here we report on the first two cloned CiV genes, which are named CiV14g1 and CiV14g2 because of their localization on segment CiV14. The cDNA of CiV14g1 has a size of 2036 bp; the gene contains seven exons interrupted by six introns of similar size and encodes a putative polypeptide of 548 amino acids. The cDNA of CiV14g2 has a size of 618 bp; the gene consists of three exons and encodes a putative peptide of 77 amino acids. Transcript quantities of both genes are very low up to the penultimate larval instar of the host. In the last instar, at the stage of pupal cell formation, CiV14g1 expression increases about 5-fold and CiV14g2 expression about a 1000-fold. These are the first data to show strong upregulation of polydnavirus genes towards the end of parasitization. These two genes might be involved in the reduction of host ecdysteroids observed at this stage.
Assuntos
Genes Virais , Himenópteros/virologia , Polydnaviridae/genética , Spodoptera/parasitologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Northern Blotting , Southern Blotting , Regulação Viral da Expressão Gênica , Biblioteca Gênica , Dados de Sequência MolecularRESUMO
Polydnaviruses (genera Ichnovirus and Bracovirus) have a segmented genome of circular double-stranded DNA molecules, replicate in the ovary of parasitic wasps and are essential for successful parasitism of the host. Here we show the first detailed analysis of various segments of a bracovirus, the Chelonus inanitus virus (CiV). Four segments were sequenced and two of them, CiV12 and CiV14, were found to be closely related while CiV14.5 and CiV16.8 were unrelated. CiV12, CiV14.5 and CiV16.8 are unique while CiV14 occurs also nested in another larger segment. All four segments are predicted to contain genes and predictions could be substantiated in most cases. Comparison with databases revealed no significant similarities at either the nucleotide or amino acid level. Inverted repeats with identities between 77% and 92% and lengths between 26 bp and 100 bp were found on all segments outside of predicted genes. Hybridization experiments indicate that CiV12 and CiV14 are both flanked by other virus segments, suggesting that proviral CiV segments are clustered in the genome of the wasp. The integration/excision site of CiV14 was analysed and compared to that of CiV12. On both termini of proviral CiV12 and CiV14 as well as in the excised circular molecule and the rejoined DNA a very similar repeat of 14 bp was found. A model to illustrate where the terminal repeats might recombine to yield the circular molecule is presented. Excision of CiV12 and CiV14 is restricted to the female and sets in at a very specific time-point in pupal-adult development.
Assuntos
Polydnaviridae/genética , Vespas/virologia , Animais , Sequência de Bases , Sítios de Ligação , DNA Viral , Feminino , Dados de Sequência Molecular , Família Multigênica , Análise de Sequência de DNA , Spodoptera/parasitologia , Integração ViralRESUMO
Polydnaviruses are unique symbiotic viruses that are replicated in the calyx cells of the ovary of some parasitic wasps. They have a segmented genome of circular double-stranded DNA and are injected along with the wasp's egg into the host, where they are essential for successful parasitism. Polydnaviruses replicate from integrated proviral DNA, and after excision of viral segments, flanking DNA is rejoined. Little is known about ovarian morphogenesis, the mode of amplification of the viral DNA and the involvement of ecdysteroids. Here we have analysed these parameters in the course of pupal-adult development in the braconid wasp Chelonus inanitus. Immediately after pupation, ovarian cells proliferated and calyx cells began to differentiate; at this stage ecdysteroids, in particular 20-hydroxyecdysone, were highest. Thereafter, calyx cells began to increase in size and DNA content and eventually became gigantic. Amplification of non-viral DNA (actin) and viral DNA in its integrated and excised form and of corresponding rejoined flanking regions was measured by quantitative real-time PCR. In the early phase of calyx cell differentiation, copy numbers of actin and integrated viral DNA increased to a similar extent. This, along with the increase in nuclear volume and DNA content in the absence of extensive cell proliferation, suggested polyploidization of the early stage calyx cells. In the following phase, integrated viral DNA was selectively and intensively amplified and eventually excised and circularized. As copy numbers of excised circular viral DNA and rejoined flanking DNA reached similarly high levels, excised viral DNA appeared not to replicate. After adult eclosion, amplification of viral DNA declined.