Non-linear properties of the Achilles tendon determine ankle impedance over a broad range of activations in humans.

Regulating ankle mechanics is essential for controlled interactions with the environment and rejecting unexpected disturbances. Ankle mechanics can be quantified by impedance, the dynamic relationship between an imposed displacement and the torque generated in response. Ankle impedance in the sagittal plane depends strongly on the triceps surae and Achilles tendon, but their relative contributions remain unknown. It is commonly assumed that ankle impedance is controlled by changing muscle activation and, thereby, muscle impedance, but this ignores that tendon impedance also changes with activation-induced loading. Thus, we sought to determine the relative contributions from the triceps surae and Achilles tendon during conditions relevant to postural control. We used a novel technique that combines B-mode ultrasound imaging with joint-level perturbations to quantify ankle, muscle and tendon impedance simultaneously across activation levels from 0% to 30% of maximum voluntary contraction. We found that muscle and tendon stiffness, the static component of impedance, increased with voluntary plantarflexion contractions, but that muscle stiffness exceeded tendon stiffness at very low loads (21±7 N). Above these loads, corresponding to 1.3% of maximal strength for an average participant in our study, ankle stiffness was determined predominately by Achilles tendon stiffness. At approximately 20% MVC for an average participant, ankle stiffness was 4 times more sensitive to changes in tendon stiffness than to changes in muscle stiffness. We provide the first empirical evidence demonstrating that the nervous system, through changes in muscle activations, leverages the non-linear properties of the Achilles tendon to increase ankle stiffness during postural conditions.

Influence of limb position on assessment of nerve mechanical properties by using shear wave ultrasound elastography.

INTRODUCTION: Evaluation of nerve mechanical properties has the potential to improve assessment of nerve impairment. Shear wave velocity, as measured by using shear wave (SW) ultrasound elastography, is a promising indicator of nerve mechanical properties such as stiffness. However, elucidation of external factors that influence SW velocity, particularly nerve tension, is required for accurate interpretations. METHODS: Median and ulnar nerve SW velocities were measured at proximal and distal locations with limb positions that indirectly altered nerve tension. RESULTS: Shear wave velocity was greater at proximal and distal locations for limb positions that induced greater tension in the median (mean increase proximal 89.3%, distal 64%) and ulnar (mean increase proximal 91.1%, distal 37.4%) nerves. DISCUSSION: Due to the influence of nerve tension when SW ultrasound elastography is used, careful consideration must be given to limb positioning.

Conference report on contractures in musculoskeletal and neurological conditions.

Limb contractures are debilitating complications associated with various muscle and nervous system disorders. This report summarizes presentations at a conference at the Shirley Ryan AbilityLab in Chicago, Illinois, on April 19-20, 2018, involving researchers and physicians from diverse disciplines who convened to discuss current clinical and preclinical understanding of contractures in Duchenne muscular dystrophy, stroke, cerebral palsy, and other conditions. Presenters described changes in muscle architecture, activation, extracellular matrix, satellite cells, and muscle fiber sarcomeric structure that accompany or predispose muscles to contracture. Participants identified ongoing and future research directions that may lead to understanding of the intersecting factors that trigger contractures. These include additional studies of changes in muscle, tendon, joint, and neuronal tissues during contracture development with imaging, molecular, and physiologic approaches. Participants identified the requirement for improved biomarkers and outcome measures to identify patients likely to develop contractures and to accurately measure efficacy of treatments currently available and under development.

Skeletal muscle mechanics, energetics and plasticity.

The following papers by Richard Lieber (Skeletal Muscle as an Actuator), Thomas Roberts (Elastic Mechanisms and Muscle Function), Silvia Blemker (Skeletal Muscle has a Mind of its Own: a Computational Framework to Model the Complex Process of Muscle Adaptation) and Sabrina Lee (Muscle Properties of Spastic Muscle (Stroke and CP) are summaries of their representative contributions for the session on skeletal muscle mechanics, energetics and plasticity at the 2016 Biomechanics and Neural Control of Movement Conference (BANCOM 2016). Dr. Lieber revisits the topic of sarcomere length as a fundamental property of skeletal muscle contraction. Specifically, problems associated with sarcomere length non-uniformity and the role of sarcomerogenesis in diseases such as cerebral palsy are critically discussed. Dr. Roberts then makes us aware of the (often neglected) role of the passive tissues in muscles and discusses the properties of parallel elasticity and series elasticity, and their role in muscle function. Specifically, he identifies the merits of analyzing muscle deformations in three dimensions (rather than just two), because of the potential decoupling of the parallel elastic element length from the contractile element length, and reviews the associated implications for the architectural gear ratio of skeletal muscle contraction. Dr. Blemker then tackles muscle adaptation using a novel way of looking at adaptive processes and what might drive adaptation. She argues that cells do not have pre-programmed behaviors that are controlled by the nervous system. Rather, the adaptive responses of muscle fibers are determined by sub-cellular signaling pathways that are affected by mechanical and biochemical stimuli; an exciting framework with lots of potential. Finally, Dr. Lee takes on the challenging task of determining human muscle properties in vivo. She identifies the dilemma of how we can demonstrate the effectiveness of a treatment, specifically in cases of muscle spasticity following stroke or in children with cerebral palsy. She then discusses the merits of ultrasound based elastography, and the clinical possibilities this technique might hold. Overall, we are treated to a vast array of basic and clinical problems in skeletal muscle mechanics and physiology, with some solutions, and many suggestions for future research.

Aging Does Not Alter Ankle, Muscle, and Tendon Stiffness at Low Loads Relevant to Stance.

Older adults have difficulty maintaining balance when faced with postural disturbances, a task that is influenced by the stiffness of the triceps surae and Achilles tendon. Age-related changes in Achilles tendon stiffness have been reported at matched levels of effort, but measures typically have not been made at matched loads, which is important due to age-dependent changes in strength. Moreover, there has been limited investigation into age-dependent changes in muscle stiffness. Here, we investigate how age alters muscle and tendon stiffness and their influence on ankle stiffness. We hypothesized that age-related changes in muscle and tendon contribute to reduced ankle stiffness in older adults and evaluated this hypothesis when either load or effort were matched. We used B-mode ultrasound with joint-level perturbations to quantify ankle, muscle, and tendon stiffness across a range of loads and efforts in seventeen healthy younger and older adults. At matched loads relevant to standing and the stance phase of walking, there was no significant difference in ankle, muscle, or tendon stiffness between groups (all p > 0.13). However, at matched effort, older adults exhibited a significant decrease in ankle (27%; p = 0.008), muscle (37%; p = 0.02), and tendon stiffness (22%; p = 0.03) at 30% of maximum effort. This is consistent with our finding that older adults were 36% weaker than younger adults in plantarflexion (p = 0.004). Together, these results indicate that, at the loads tested in this study, there are no age-dependent changes in the mechanical properties of muscle or tendon, only differences in strength that result in altered ankle, muscle, and tendon stiffness at matched levels of effort.

Recruitment of faster motor units is associated with greater rates of fascicle strain and rapid changes in muscle force during locomotion.

Animals modulate the power output needed for different locomotor tasks by changing muscle forces and fascicle strain rates. To generate the necessary forces, appropriate motor units must be recruited. Faster motor units have faster activation-deactivation rates than slower motor units, and they contract at higher strain rates; therefore, recruitment of faster motor units may be advantageous for tasks that involve rapid movements or high rates of work. This study identified motor unit recruitment patterns in the gastrocnemii muscles of goats and examined whether faster motor units are recruited when locomotor speed is increased. The study also examined whether locomotor tasks that elicit faster (or slower) motor units are associated with increased (or decreased) in vivo tendon forces, force rise and relaxation rates, fascicle strains and/or strain rates. Electromyography (EMG), sonomicrometry and muscle-tendon force data were collected from the lateral and medial gastrocnemius muscles of goats during level walking, trotting and galloping and during inclined walking and trotting. EMG signals were analyzed using wavelet and principal component analyses to quantify changes in the EMG frequency spectra across the different locomotor conditions. Fascicle strain and strain rate were calculated from the sonomicrometric data, and force rise and relaxation rates were determined from the tendon force data. The results of this study showed that faster motor units were recruited as goats increased their locomotor speeds from level walking to galloping. Slow inclined walking elicited EMG intensities similar to those of fast level galloping but different EMG frequency spectra, indicating that recruitment of the different motor unit types depended, in part, on characteristics of the task. For the locomotor tasks and muscles analyzed here, recruitment patterns were generally associated with in vivo fascicle strain rates, EMG intensity and tendon force. Together, these data provide new evidence that changes in motor unit recruitment have an underlying mechanical basis, at least for certain locomotor tasks.

Axial stress determines the velocity of shear wave propagation in passive but not active muscles in vivo.

Ultrasound shear wave elastography can be used to characterize mechanical properties of unstressed tissue by measuring shear wave velocity (SWV), which increases with increasing tissue stiffness. Measurements of SWV have often been assumed to be directly related to the stiffness of muscle. Some have also used measures of SWV to estimate stress, since muscle stiffness and stress covary during active contractions, but few have considered the direct influence of muscle stress on SWV. Rather, it is often assumed that stress alters the material properties of muscle, and in turn, shear wave propagation. The objective of this study was to determine how well the theoretical dependency of SWV on stress can account for measured changes of SWV in passive and active muscles. Data were collected from six isoflurane-anesthetized cats; three soleus muscles and three medial gastrocnemius muscles. Muscle stress and stiffness were measured directly along with SWV. Measurements were made across a range of passively and actively generated stresses, obtained by varying muscle length and activation, which was controlled by stimulating the sciatic nerve. Our results show that SWV depends primarily on the stress in a passively stretched muscle. In contrast, the SWV in active muscle is higher than would be predicted by considering only stress, presumably due to activation-dependent changes in muscle stiffness. Our results demonstrate that while SWV is sensitive to changes in muscle stress and activation, there is not a unique relationship between SWV and either of these quantities when considered in isolation.NEW & NOTEWORTHY Ultrasound shear wave elastography may be an inexpensive way to measure muscle stress in passive muscle. Here, using a cat model we directly measured shear wave velocity (SWV), muscle stress, and muscle stiffness. Our results show that SWV depends primarily on the stress in a passively stretched muscle. In contrast, the SWV in active muscle is higher than would be predicted by considering only stress, presumably due to activation-dependent changes in muscle stiffness.

At matched loads, aging does not alter ankle, muscle, or tendon stiffness.

Older adults have difficulty maintaining balance when faced with postural disturbances, a task that is influenced by the stiffness of the triceps surae and Achilles tendon. Age-related changes in Achilles tendon stiffness have been reported at matched levels of effort, but measures typically have not been made at matched loads, which is important due to age-dependent changes in strength. Moreover, age-dependent changes in muscle stiffness have yet to be tested. Here, we investigate how age alters muscle and tendon stiffness and their influence on ankle stiffness. We hypothesized that age-related changes in muscle and tendon contribute to reduced ankle stiffness in older adults and evaluated this hypothesis when either load or effort were matched. We used B-mode ultrasound with joint-level perturbations to quantify ankle, muscle, and tendon stiffness across a range of loads and efforts in seventeen healthy younger and older adults. At matched loads, there was no significant difference in ankle, muscle, or tendon stiffness between groups (all p>0.13). However, at matched effort, older adults exhibited a significant decrease in ankle (27%; p=0.008), muscle (37%; p=0.02), and tendon stiffness (22%; p=0.03) at 30% of maximum effort. This is consistent with our finding that older adults were 36% weaker than younger adults in plantarflexion (p=0.004). Together these results indicate that, at the loads tested in this study, there are no age-dependent changes in the mechanical properties of muscle or tendon, only differences in strength that result in altered ankle, muscle, and tendon stiffness at matched levels of effort.

Simultaneous Quantification of Ankle, Muscle, and Tendon Impedance in Humans.

OBJECTIVE: Regulating the impedance of our joints is essential for the effective control of posture and movement. The impedance of a joint is governed mainly by the mechanical properties of the muscle-tendon units spanning it. Many studies have quantified the net impedance of joints but not the specific contributions from the muscles and tendons. The inability to quantify both muscle and tendon impedance limits the ability to determine the causes underlying altered movement control associated with aging, neuromuscular injury, and other conditions that have different effects on muscle and tendon properties. Therefore, we developed a technique to quantify joint, muscle, and tendon impedance simultaneously and evaluated this technique at the human ankle. METHODS: We used a single degree of freedom actuator to deliver pseudorandom rotations to the ankle while measuring the corresponding torques. We simultaneously measured the displacement of the medial gastrocnemius muscle-tendon junction with B-mode ultrasound. From these experimental measurements, we were able to estimate ankle, muscle, and tendon impedance using non-parametric system identification. RESULTS: We validated our estimates by comparing them to previously reported measurements of muscle and tendon stiffness, the position-dependent component of impedance, to demonstrate that our technique generates reliable estimates of these properties. CONCLUSION: Our approach can be used to clarify the respective contributions from the muscle and tendon to the net mechanics of a joint. SIGNIFICANCE: This is a critical step forward in the ultimate goal of understanding how muscles and tendons govern ankle impedance during posture and movement.

Mapping the relationships between joint stiffness, modeled muscle stiffness, and shear wave velocity.

Joint stiffness is often measured to make inferences about the stiffness of muscle groups, but little can be gleaned about individual muscles. Decomposing the muscular origins of joint stiffness may inform treatment targets for conditions like spasticity. To complement joint stiffness, shear wave ultrasound elastography has been used to estimate the material properties of individual muscles. If shear wave measures are to be used to assess the muscular origins of joint stiffness, then changes in shear wave velocity should strongly relate to changes in joint stiffness. Here, we estimated the relationships between shear wave velocity in the primary plantar flexors [soleus (SOL) and medial gastrocnemius (MG)] and ankle joint stiffness. Participants performed isometric plantar flexion tasks at a range of activations (0-40%), while joint stiffness and muscle shear wave velocities were obtained. We observed a strong, linear relationship between plantar flexor shear wave velocities and joint stiffness. Remarkably, the parameter estimates of this stiffness-shear wave relationship strongly agreed with theoretical and literature-based estimates [SOL:MG parameter ratios = 2.83 (observed) vs. 2.85 (expected from theoretical stiffness ratio)]. Finally, a musculoskeletal model of the plantar flexors was able to accurately reproduce joint stiffness estimates, and shear wave velocities could explain 80-95% of the variance in modeled muscle stiffness. These findings suggest that shear wave velocity may be used to infer the muscular origins of changes in joint stiffness.NEW & NOTEWORTHY Shear wave velocity is commonly assessed to infer the muscular origins of changes in joint stiffness, but the exact relationship between shear wave velocity changes in muscle and joint stiffness changes remains unknown. Here, we systematically evaluated and quantified this relationship in the plantar flexors. Our results provide evidence for the ability of shear wave velocity to elucidate the muscular origins of joint stiffness changes.

Limited fascicle shortening and fascicle rotation may be associated with impaired voluntary force-generating capacity in pennate muscles of chronic stroke survivors.

BACKGROUND: Muscle weakness is one of the most common motor impairments after stroke. A variety of progressive muscular changes are reported in chronic stroke survivors, and it is now feasible to consider these changes as an added source of weakness. However, the net contributions of such muscular changes towards muscle weakness have not been fully quantified. METHODS: Accordingly, this study aims: (1) to compare muscle architecture of the human medial gastrocnemius between paretic and non-paretic sides in seven chronic hemispheric stroke survivors under passive conditions; (2) to characterize fascicle behavior (i.e., fascicle shortening and fascicle rotation) of the muscle during voluntary isometric contractions; and (3) to assess potential associations between muscle architectural parameters and muscle weakness. Muscle architecture of the medial gastrocnemius (including fascicle length, fascicle pennation angle, and muscle thickness) was characterized using B-mode ultrasonography, and fascicle behavior was then quantified as a function of isometric plantarflexion torque normalized to body mass. FINDINGS: Our experimental results showed that under passive conditions, there was a significant difference in fascicle length and muscle thickness between paretic and non-paretic muscles, but no difference in resting fascicle pennation angle. However, during isometric contraction, both fascicle shortening and fascicle rotation on the paretic side were significantly decreased, compared to the non-paretic side. Moreover, the relative (i.e., paretic/non-paretic) fascicle rotation-shortening ratio (i.e., fascicle rotation per fascicle shortening) was strongly correlated with the relative maximum voluntary isometric plantarflexion torque. INTERPRETATION: This association implies that such fascicle changes could impair the force-generating capacity of the muscle in chronic stroke survivors.

Simultaneous in vivo Estimation of Muscle, Tendon, and Ankle Impedance.

Appropriate regulation of joint impedance is required to successfully navigate our environment. Joint impedance is strongly dependent upon the mechanical properties of the muscles and tendons spanning it. While the impedance of the joint has been well characterized, methods to determine the individual contribution from the muscles and tendons are limited. This is a crucial gap as muscle and tendon impedance can be selectively altered by aging, pathology, or injury. Therefore, we developed an innovative in vivo method that allows for the simultaneous quantification of joint, muscle, and tendon impedance. Stochastic perturbations of ankle angle were applied while a B-mode ultrasound was used to image the displacement of the medial gastrocnemius muscle-tendon junction. Non-parametric system identification was used to quantify ankle impedance and the frequency response function between ankle rotations and muscle-tendon junction displacements. The latter represents, when scaled by Achilles tendon moment arm, the ratio between the net musculotendon impedance and the impedance of the muscle, a relationship we refer to as the impedance ratio. Muscle and tendon impedance can be calculated from these experimental estimates. The ability to simultaneously quantify joint, muscle, and tendon impedance will provide a clearer understanding their respective roles in our ability to navigate our environment, and how changes in those roles may contribute to functional impairments.

Quantifying muscle coactivation in individuals with incomplete spinal cord injury using wavelets.

BACKGROUND: Individuals with incomplete spinal cord injury often have decreased gait function and coactivation of antagonistic muscle pairs. Common ways of quantifying coactivation using electromyographic signals do not consider frequency information in the signal. As electromyographic signals from different motor unit types have different frequency components and muscle fiber type can change in individuals with spinal cord injury, it may be beneficial to consider frequency components. The aims were to demonstrate the utility of using a method which considers temporal and frequency components of the electromyographical signal to quantify coactivation in lower extremity muscles in individuals with incomplete spinal cord injury through 1) comparison with able-bodied individuals and 2) comparison before and after body weight supported treadmill training. METHODS: Frequency decomposition techniques were applied to electromyographical signals to consider the temporal and frequency components of the electromyographical signals to quantify coactivation over a range of frequencies. RESULTS: Our main findings show that correlation coefficients between total EMG intensities of rectus femoris-biceps femoris and medial gastrocnemius-tibialis anterior were significantly different between able-bodied individuals and those with incomplete spinal cord injury (p = 0006, p = 0.01). The correlation spectra of medial gastrocnemius-tibialis anterior of the spinal cord injury group were substantially different than those the able-bodied group, while the EMG normalcy score was significantly different (p = 0.002). We also found that there was a change in coactivation of ankle muscles after body weight supported treadmill training. INTERPRETATION: Our findings indicate that there may be frequency specific differences in muscle coactivation between able-bodied individuals and those with incomplete spinal cord injury. Changes in coactivation were also observed before and after body weight supported treadmill training. These differences may reflect the changes in recruitment patterns of different motor unit types.

Shear wave velocity is sensitive to changes in muscle stiffness that occur independently from changes in force.

Clinical assessments for many musculoskeletal disorders involve evaluation of muscle stiffness, although it is not yet possible to obtain quantitative estimates from individual muscles. Ultrasound elastography can be used to estimate the material properties of unstressed, homogeneous, and isotropic materials by tracking the speed of shear wave propagation; these waves propagate faster in stiffer materials. Although elastography has been applied to skeletal muscle, there is little evidence that shear wave velocity (SWV) can directly estimate muscle stiffness since this tissue violates many of the assumptions required for there to be a direct relationship between SWV and stiffness. The objective of this study was to evaluate the relationship between SWV and direct measurements of muscle force and stiffness in contracting muscle. Data were collected from six isoflurane-anesthetized cats. We measured the short-range stiffness in the soleus via direct mechanical testing in situ and SWV via ultrasound imaging. Measurements were taken during supramaximal activation at optimum muscle length, with muscle temperature varying between 26°C and 38°C. An increase in temperature causes a decrease in muscle stiffness at a given force, thus decoupling the tension-stiffness relationship normally present in muscle. We found that increasing muscle temperature decreased active stiffness from 4.0 ± 0.3 MPa to 3.3 ± 0.3 MPa and SWV from 16.9 ± 1.5 m/s to 15.9 ± 1.6 m/s while force remained unchanged (mean ± SD). These results demonstrate that SWV is sensitive to changes in muscle stiffness during active contractions. Future work is needed to determine how this relationship is influenced by changes in muscle structure and tension.NEW & NOTEWORTHY Shear wave ultrasound elastography is a noninvasive tool for characterizing the material properties of muscle. This study is the first to compare direct measurements of stiffness with ultrasound measurements of shear wave velocity (SWV) in a contracting muscle. We found that SWV is sensitive to changes in muscle stiffness, even when controlling for muscle tension, another factor that influences SWV. These results are an important step toward developing noninvasive tools for characterizing muscle structure and function.

Ultrasound Shear Wave Velocity Varies Across Anatomical Region in Ex Vivo Bovine Ovaries.

The physical properties of the ovarian extracellular matrix (ECM) regulate the function of ovarian cells, specifically the ability of the ovary to maintain a quiescent primordial follicle pool while allowing a subset of follicles to grow and mature in the estrous cycle. Design of a long-term, cycling artificial ovary has been hindered by the limited information regarding the mechanical properties of the ovary. In particular, differences in the mechanical properties of the two ovarian compartments, the cortex and medulla, have never been quantified. Shear wave (SW) ultrasound elastography is an imaging modality that enables assessment of material properties, such as the mechanical properties, based on the velocity of SWs, and visualization of internal anatomy, when coupled with B-mode ultrasound. We used SW ultrasound elastography to assess whole, ex vivo bovine ovaries. We demonstrated, for the first time, a difference in mechanical properties, as inferred from SW velocity, between the cortex and medulla, as measured along the length (cortex: 2.57 ± 0.53 m/s, medulla: 2.87 ± 0.77 m/s, p < 0.0001) and width (cortex: 2.99 ± 0.81 m/s, medulla: 3.24 ± 0.97 m/s, p < 0.05) and that the spatial distribution and magnitude of SW velocity vary between these two anatomical planes. This work contributes to a larger body of literature assessing the mechanical properties of the ovary and related cells and specialized ECMs and will enable the rational design of biomimetic tissue engineered models and durable bioprostheses. Impact Statement Shear wave (SW) ultrasound elastography can be used to simultaneously assess the material properties and tissue structures when accompanied with B-mode ultrasound. We report a quantitative difference in mechanical properties, as inferred from SW velocity, between the cortex and medulla, with SW velocity being 11.4% and 8.4% higher in the medulla than the cortex when measured along the length and width, respectively. This investigation into the spatial and temporal variation in SW velocity in bovine ovaries will encourage and improve design of more biomimetic scaffolds for ovarian tissue engineering.

Built for speed: musculoskeletal structure and sprinting ability.

The musculoskeletal structure of the foot and ankle has the potential to influence human sprinting performance in complex ways. A large Achilles' tendon moment arm improves the mechanical advantage of the triceps surae but also produces larger shortening velocity during rapid plantarflexion, which detracts from the force-generating capacity of the plantarflexors. The lever arm of the ground reaction force that resists the muscular plantarflexor moment during propulsive push-off is constrained in part by the skeletal structure of the foot. In this study, we measured the plantarflexion moment arms of the Achilles' tendon, lateral gastrocnemius fascicle lengths and pennation angles, and anthropometric characteristics of the foot and lower leg in collegiate sprinters and height-matched non-sprinters. The Achilles' tendon moment arms of the sprinters were 25% smaller on average in sprinters than in non-sprinters (P<0.001) whereas the sprinters' fascicles were 11% longer on average (P=0.024). The ratio of fascicle length to moment arm was 50% larger in sprinters (P<0.001). Sprinters were found to have longer toes (P=0.032) and shorter lower legs (P=0.026) than non sprinters. A simple computer simulation of the sprint push-off demonstrated that shorter plantarflexor moment arms and longer toes, like those measured in sprinters, permit greater generation of forward impulse. Simulated propulsion was enhanced in both cases by increasing the ;gear ratio' of the foot, thus maintaining plantarflexor fibre length and reducing peak fibre shortening velocity. Longer toes especially prolonged the time of contact, giving greater time for forward acceleration by propulsive ground reaction force.

Muscle material properties in passive and active stroke-impaired muscle.

Stroke survivors routinely experience long-term motor and sensory impairments. In parallel with neurological changes, material properties of muscles in the impaired limbs, such as muscle stiffness, may also change progressively. However, these stiffness measures are routinely derived from individual joint stiffness, representing whole muscle groups. Here, we use shear wave (SW) ultrasound elastography to measure SW velocity, as a surrogate measure of stiffness, to quantify material properties in individual muscles. Accordingly, the purpose of this study was to compare muscle material properties of the bicep brachii in stroke survivors and in age-matched control subjects by measuring SW velocity at rest and different voluntary activation levels. Our main findings show that at rest, the SW velocity was on average 41% greater in the paretic muscle compared the contralateral non-paretic muscle. The mean passive SW velocity across all subjects were 2.34 ±â€¯0.41 m/s for the non-paretic side, 3.30 ±â€¯1.20 m/s for the paretic side, and 2.24 ±â€¯0.18 for controls. SW velocity was significantly different in muscles of the paretic and non-paretic side (p < 0.001), but not between muscles of the non-paretic and controls (p = 0.47). As voluntary activation increased, SW velocity increased non-linearly, with an average power fit of r2 = 0.83 ±â€¯0.09 for the non-paretic side, r2 = 0.61 ±â€¯0.24 for the paretic side, and r2 = 0.24 ±â€¯0.15 for the healthy age-matched controls. In active muscle (10, 25, 50, 75, 100% maximum voluntary contraction), there was no significant difference in SW velocity between the non-paretic, paretic, and control muscles. These findings suggest that stroke-impaired muscles have potentially altered muscle material properties, specifically stiffness, and that passive and active stiffness may contribute differently to total muscle stiffness.

Changes in shear wave propagation within skeletal muscle during active and passive force generation.

Muscle force can be generated actively through changes in neural excitation, and passively through externally imposed changes in muscle length. Disease and injury can disrupt force generation, but it can be challenging to separate passive from active contributions to these changes. Ultrasound elastography is a promising tool for characterizing the mechanical properties of muscles and the forces that they generate. Most prior work using ultrasound elastography in muscle has focused on the group velocity of shear waves, which increases with increasing muscle force. Few studies have quantified the phase velocity, which depends on the viscoelastic properties of muscle. Since passive and active forces within muscle involve different structures for force transmission, we hypothesized that measures of phase velocity could detect changes in shear wave propagation during active and passive conditions that cannot be detected when considering only group velocity. We measured phase and group velocity in the human biceps brachii during active and passive force generation and quantified the differences in estimates of shear elasticity obtained from each of these measurements. We found that measures of group velocity consistently overestimate the shear elasticity of muscle. We used a Voigt model to characterize the phase velocity and found that the estimated time constant for the Voigt model provided a way to distinguish between passive and active force generation. Our results demonstrate that shear wave elastography can be used to distinguish between passive and active force generation when it is used to characterize the phase velocity of shear waves propagating in muscle.

Inversion-eversion moment arms of gastrocnemius and tibialis anterior measured in vivo.

In this study, the frontal plane moment arms of tibialis anterior (TA) and the lateral and medial heads of gastrocnemius (LG and MG) were determined using ultrasonography of ten healthy subjects. Analysis of variance was performed to investigate the effects of frontal plane angle, muscle activity, and plantarflexion angle on inversion-eversion moment arm for each muscle. The moment arms of each muscle were found to vary with frontal plane angle (all p<0.001). TA and LG exhibited eversion moment arms when the foot was everted, but MG was found to have a slight inversion moment arm in this position. As the ankle rotated from 0 degrees to 20 degrees inversion, the inversion moment arm of each increased, indicating that the three muscles became increasingly effective inverters. In neutral position, the inverter moment arm of MG was greater than that of LG (p=0.001). Muscle activity had a significant effect on both LG and MG moment arm at all frontal plane positions (all p0.005). These results demonstrate the manner in which frontal plane moment arms of gastrocnemius and TA differ across the frontal plane range of motion in healthy subjects. This method for assessing muscle action in vivo used in this study may prove useful for subject-specific planning of surgical treatments for frontal plane foot and ankle deformities.

Shear Waves Reveal Viscoelastic Changes in Skeletal Muscles After Hemispheric Stroke.

We investigated alterations in material properties such as elasticity and viscoelasticity of stroke-affected muscles using ultrasound induced shear waves and mechanical models. We used acoustic radiation force to generate shear waves along fascicles of biceps muscles and measured their propagation velocity. The shear wave data were collected in muscles of 13 hemiplegic stroke survivors under passive conditions at 90°, 120°, and 150° elbow flexion angles. In a viscoelastic medium, as opposed to a purely elastic medium, the shear wave propagation velocity depends on the frequency content of the induced wave. Therefore, in addition to the shear wave group velocity (GpV), we also estimated a frequency-dependent phase velocity (PhV). We found significantly higher GpVs and PhVs in stroke-affected muscles ( ). The velocity data were used to estimate shear elasticity and viscosity using an elastic and viscoelastic material models. A pure elastic model showed increased shear elasticity in stroke-affected muscles ( ). The Voigt model estimates of viscoelastic properties were also significantly different between the stroke-impaired and non-impaired muscles. We observed significantly larger model-estimated viscosity values on the stroke-affected side at elbow flexion angles of 120° and 150°. Furthermore, the creep behavior (tissue strain resulting from the application of sudden constant stress) of the model was also different between muscles of the paretic and non-paretic side. We speculate that these changes are associated with the structural disruption of muscles after stroke and may potentially affect force generation from muscle fibers as well as transmission of force to tendons.