The impact of glycine and glutamate, as components of glutathione precursors, on the productivity, digestive performance and blood profile of weaning pigs.

The impact of glycine and glutamate, as components of glutathione (GSH) precursors, was studied as a factor in determining the growth rate of weaning pigs, their digestion of nutrient supplements and their blood concentration levels. There were 180 crossbred weaning pigs with an average body weight (BW) of 7.94 ± 1.53 kg (five pigs per pen [two barrows and three gilts]; nine pens per treatment) that were randomly assigned to one of four diets. We used a basal diet as the control, TRT1 as the treatment with 0.10% precursor of GSH, TRT2 as the treatment with 0.20% precursor of GSH and TRT3 as the treatment with 0.30% precursor of GSH. The BW of weaning pigs exhibited a linear increase on days 7 (p < 0.001), 21 (p < 0.001) and 42 (p < 0.009) following the supplementation with the GSH precursor. Supplementation with GSH precursor led to a consistent and gradual increase in average daily gain (ADG) on days 8-21, 22-42 and overall, as indicated by a significant linear trend (p < 0.05). G: F was linearly increased (p < 0.05) on days 22-42 and overall with the increment in the precursor of GSH supplementation. However, GSH precursor supplementation did not have any impact on nutrient digestibility and blood profile in the treatment group. In summary, the administration of GSH precursor improved BW, ADG and G: F ratio while not affecting nutrient digestibility or blood profile.

Effects of supplementation of graded level of glutamic acid to crude protein reduced diets on the performance of growing pigs.

A total of 150 growing pigs ([Landrace × Yorkshire] × Duroc) with an initial average body weight (BW) of 24.45 kg were used in a 6-week trial to estimate the optimum lysine to glutamic acid ratio in pigs fed low-protein diets supplemented with increasing level of synthetic glutamic acid (Glu). Pigs were randomly allotted to 5 dietary treatments consisting of either control diet (CON) formulated to have 157 g crude protein (CP) or negative control diets (NC, NC1, NC2 and NC3) with 20 g CP reduction and addition of Glu (1.1, 3.9, 6.8 and 9.6 g/kg feed respectively). Supplementing the increasing level of Glu to low CP diets did not exert any linear or quadratic responses in the growth performance parameters as well as nutrient digestibility. The serum creatinine concentration in pigs receiving CON diet showed trends (p = 0.063) in increment compared with pigs receiving NC diet. However, with the increase in the supplementation of Glu, there were no linear or quadratic responses on serum blood urea nitrogen (BUN) and creatinine concentrations. There was a tendency in the reduction (p = 0.088, p = 0.064) of backfat thickness and lean percentage, respectively, at week 3 and a trend in the reduction (p = 0.092) in lean percentage at week 6 in pigs fed NC diet compared with those fed CON diet. The increase in the supplemental level of Glu tended to show quadratic responses in the backfat thickness and lean percentage at week 3 and 6. In conclusion, the growth performance parameters as well as carcass traits with Lys: Glu ratio 1: 2.71 were very close with the mean values of CON diet indicating that 6.8 g Glu when supplemented to 2% CP reduced diet could achieve the comparable growth performance and carcass trait as that of standard basal diet.

Glutamic acid supplementation recovers the reduced performance of weanling pigs fed reduced crude protein diets.

This study was conducted to evaluate the supplementation of glutamic acid (Glu) to reduced protein diets on the performance of weanling pigs. One hundred and eighty crossbred weanling pigs ([Yorkshire × Landrace] × Duroc, 21 d old) having similar body weight (BW) of 6.45 kg were randomly allotted to 1 of 6 dietary treatments (5 pigs per pen [2 barrows and 3 gilts]; 6 pens per treatment) based on BW and sex during a 6-week trial. Dietary treatments consisted of positive control (PC) diet formulated to have 226.9, 205.6, and 188.8 g crude protein (CP) during phases 1, 2, and 3, respectively, and negative control (NC) diets with 20 g CP reduction from PC diets and addition of Glu with increasing levels, resulting in the calculated Lys-to-Glu ratios of 1:2.25, 1:2.30.1:2.35, 1:2.40, and 1:2.45, designated as NC, NC1, NC2, NC3, and NC4, respectively. The BW of pigs receiving PC diet was higher (P < 0.05) than those receiving NC diet at d 7, 21 and 42. A higher (P < 0.05) average daily gain (ADG) from d 1 to 7, 8 to 21, 22 to 42 and during the overall experiment period was observed in pigs fed PC than NC diet. Pigs fed NC diets including the graded level of Glu linearly increased (P < 0.05) BW at d 42, ADG and gain-to-feed ratio (G:F) during the overall experimental period. In addition, trends in linear increase in BW (P = 0.056) at d 7 and ADG from d 1 to 7 and d 22 to 42 (linear effect, P = 0.081, P = 0.058 respectively) were observed. A tendency in the linear increment of NH3 (P = 0.082) at d 21 and linear reduction in methyl mercaptans (P = 0.054) emission at d 42 was observed in pigs fed NC diets supplemented with graded level of Glu. In conclusion, supplementing the reduced protein diet with Glu enhanced the growth performance in weanling pigs suggesting that supplementation of Glu can compensate the reduction of 2% CP in the basal diets.

Effect of increasing levels of threonine relative to lysine on the performance and meat quality of finishing pigs.

OBJECTIVE: The present study aimed to evaluate the effects of varying standardized ileal digestible lysine:threonine (SID Lys:Thr) ratio in the diet on the performance and meat quality of finishing pigs. METHODS: In total 192 crossbred pigs ([Landrace×Yorkshire]×Duroc, 17 weeks old), with an initial body weight (BW) of 70.6±3.9 kg were used in an 8-wk trial. Pigs were randomly allotted to one of six dietary treatments based on their initial BW and sex (8 replications; 4 pigs per pen, 2 barrows and 2 gilts). The pigs in the 6 treatments were fed diets having different SID Lys:Thr ratios such as 1:0.65, 1:0.66, 1:0.67, 1:0.68, 1:0.69, and 1:0.70. RESULTS: A linear increment (p<0.05) in average daily gain (ADG) and trends in reduction in feed conversion ratio (FCR) were observed during day 29 to 56 of the experiment and the apparent total tract digestibility (ATTD) of dry matter tended to increase linearly (p = 0.094) at the end of the experiment (day 56) with the increase in the dietary SID Lys:Thr ratios. The backfat thickness and lean percentage increased (linear effect, p<0.05) on day 28. In addition, at day 56, a linear (p<0.05) increment in lean percentage was observed. Significant quadratic responses (p = 0.02) for pH and drip loss at day 7 (p = 0.02), a linear increase (p<0.05) in cooking loss and drip loss at day 7, and a trend in quadratic response (p = 0.07) in the lightness of meat color (L*) were observed, whereas other meat quality indices were unaffected by varying the SID Lys:Thr ratios. CONCLUSION: The SID Lys:Thr ratio for maximum ADG, minimum FCR and enhanced digestibility was found to be 0.70. However, for carcass trait and meat quality, the SID Lys:Thr ratio of 0.65 was enough.

Chemotherapy and interferon-alpha treatment of Erdheim-Chester disease.

Erdheim-Chester disease (ECD) is a rare non-Langerhans cell histiocytosis of an unknown origin. The prognosis of ECD is variable, and it mainly depends on the involved anatomic sites. The treatment modalities have not been standardized. Interferon-alpha (IFN) has been reported to be effective in the management of ECD. We report here on an uncommon case with ECD in a 17-year-old female who had multiple lesions in the whole body and she was treated with chemotherapy and IFN. She has remained disease-free for 2 years after the completion of treatment.

A case of congenital duodenal web causing duodenal stenosis in a down syndrome child: endoscopic resection with an insulated-tip knife.

A 35-month-old girl visited our hospital with repetitive vomiting and abdominal distention; this was especially aggravated after the introduction of solid and semisolid foods. At 5 months of age, the patient, who had Down's syndrome, had undergone surgery for ventricular septal defect, atrial septal defect, and patent ductus arteriosus, and had subsequently been frequently hospitalized for respiratory infections and other viral infectious diseases. After her admission, the abdominal distension improved with fasting and intravenous fl uid therapy. Radiograph from a small-bowel series revealed a thin fi lling defect with a dilated duodenal bulb in the distal region of the second portion of the duodenum, suggesting a duodenal web, and endoscopy revealed duodenal stenosis. We therefore performed endoscopic resection with an insulated-tip knife because of the history of prior operations, fasting problems after operations, and respiratory infections. Seven days later, scar formation was noted on the second portion of the duodenum, the scope passed well at the excision site, and no retained food material was noted on the follow-up endoscopy. After the procedure, the patient's abdominal distention and repetitive vomiting subsided, and she was discharged with the ability to eat eat an age-appropriate normal diet. There were no specifi c symptoms or other complications for 1 year after the procedure.