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1.
Nature ; 589(7841): 306-309, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33208949

RESUMO

CrAss-like phages are a recently described expansive group of viruses that includes the most abundant virus in the human gut1-3. The genomes of all crAss-like phages encode a large virion-packaged protein2,4 that contains a DFDxD sequence motif, which forms the catalytic site in cellular multisubunit RNA polymerases (RNAPs)5. Here, using Cellulophaga baltica crAss-like phage phi14:2 as a model system, we show that this protein is a DNA-dependent RNAP that is translocated into the host cell along with the phage DNA and transcribes early phage genes. We determined the crystal structure of this 2,180-residue enzyme in a self-inhibited state, which probably occurs before virion packaging. This conformation is attained with the help of a cleft-blocking domain that interacts with the active site and occupies the cavity in which the RNA-DNA hybrid binds. Structurally, phi14:2 RNAP is most similar to eukaryotic RNAPs that are involved in RNA interference6,7, although most of the phi14:2 RNAP structure (nearly 1,600 residues) maps to a new region of the protein fold space. Considering this structural similarity, we propose that eukaryal RNA interference polymerases have their origins in phage, which parallels the emergence of the mitochondrial transcription apparatus8.


Assuntos
Bacteriófagos/classificação , Bacteriófagos/enzimologia , RNA Polimerases Dirigidas por DNA/química , RNA Polimerases Dirigidas por DNA/metabolismo , Flavobacteriaceae/virologia , Bacteriófagos/genética , Domínio Catalítico , Sistema Livre de Células , Cristalografia por Raios X , DNA de Cadeia Simples/biossíntese , DNA de Cadeia Simples/genética , RNA Polimerases Dirigidas por DNA/genética , Evolução Molecular , Regulação Viral da Expressão Gênica , Genes Virais/genética , Modelos Biológicos , Modelos Moleculares , Domínios Proteicos , Subunidades Proteicas/química , Subunidades Proteicas/metabolismo , Interferência de RNA , Transcrição Gênica
2.
Nature ; 580(7805): 658-662, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32350467

RESUMO

R-type bacteriocins are minimal contractile nanomachines that hold promise as precision antibiotics1-4. Each bactericidal complex uses a collar to bridge a hollow tube with a contractile sheath loaded in a metastable state by a baseplate scaffold1,2. Fine-tuning of such nucleic acid-free protein machines for precision medicine calls for an atomic description of the entire complex and contraction mechanism, which is not available from baseplate structures of the (DNA-containing) T4 bacteriophage5. Here we report the atomic model of the complete R2 pyocin in its pre-contraction and post-contraction states, each containing 384 subunits of 11 unique atomic models of 10 gene products. Comparison of these structures suggests the following sequence of events during pyocin contraction: tail fibres trigger lateral dissociation of baseplate triplexes; the dissociation then initiates a cascade of events leading to sheath contraction; and this contraction converts chemical energy into mechanical force to drive the iron-tipped tube across the bacterial cell surface, killing the bacterium.


Assuntos
Pseudomonas aeruginosa , Piocinas/química , Piocinas/metabolismo , Bacteriófago T4/química , Bacteriófago T4/metabolismo , Microscopia Crioeletrônica , Cristalografia por Raios X , Genes Bacterianos/genética , Modelos Moleculares , Subunidades Proteicas/química , Subunidades Proteicas/genética , Subunidades Proteicas/metabolismo , Pseudomonas aeruginosa/química , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/metabolismo , Especificidade por Substrato , Sistemas de Secreção Tipo VI/química , Sistemas de Secreção Tipo VI/metabolismo
3.
EMBO J ; 38(3)2019 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-30606715

RESUMO

Contractile injection systems (bacteriophage tails, type VI secretions system, R-type pyocins, etc.) utilize a rigid tube/contractile sheath assembly for breaching the envelope of bacterial and eukaryotic cells. Among contractile injection systems, bacteriophages that infect Gram-positive bacteria represent the least understood members. Here, we describe the structure of Listeria bacteriophage A511 tail in its pre- and post-host attachment states (extended and contracted, respectively) using cryo-electron microscopy, cryo-electron tomography, and X-ray crystallography. We show that the structure of the tube-baseplate complex of A511 is similar to that of phage T4, but the A511 baseplate is decorated with different receptor-binding proteins, which undergo a large structural transformation upon host attachment and switch the symmetry of the baseplate-tail fiber assembly from threefold to sixfold. For the first time under native conditions, we show that contraction of the phage tail sheath assembly starts at the baseplate and propagates through the sheath in a domino-like motion.


Assuntos
Bacteriófagos/fisiologia , Bacteriófagos/ultraestrutura , Listeria/virologia , Conformação Proteica , Proteínas da Cauda Viral/química , Microscopia Crioeletrônica , Cristalografia por Raios X , Infecções , Listeria/crescimento & desenvolvimento , Modelos Moleculares , Proteínas da Cauda Viral/metabolismo
4.
Nature ; 533(7603): 346-52, 2016 05 19.
Artigo em Inglês | MEDLINE | ID: mdl-27193680

RESUMO

Several systems, including contractile tail bacteriophages, the type VI secretion system and R-type pyocins, use a multiprotein tubular apparatus to attach to and penetrate host cell membranes. This macromolecular machine resembles a stretched, coiled spring (or sheath) wound around a rigid tube with a spike-shaped protein at its tip. A baseplate structure, which is arguably the most complex part of this assembly, relays the contraction signal to the sheath. Here we present the atomic structure of the approximately 6-megadalton bacteriophage T4 baseplate in its pre- and post-host attachment states and explain the events that lead to sheath contraction in atomic detail. We establish the identity and function of a minimal set of components that is conserved in all contractile injection systems and show that the triggering mechanism is universally conserved.


Assuntos
Bacteriófago T4/química , Bacteriófago T4/ultraestrutura , Proteínas Estruturais Virais/química , Proteínas Estruturais Virais/ultraestrutura , Microscopia Crioeletrônica , Cristalografia por Raios X , Modelos Moleculares , Conformação Proteica
5.
Proteins ; 89(12): 1633-1646, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34449113

RESUMO

Critical assessment of structure prediction (CASP) conducts community experiments to determine the state of the art in computing protein structure from amino acid sequence. The process relies on the experimental community providing information about not yet public or about to be solved structures, for use as targets. For some targets, the experimental structure is not solved in time for use in CASP. Calculated structure accuracy improved dramatically in this round, implying that models should now be much more useful for resolving many sorts of experimental difficulties. To test this, selected models for seven unsolved targets were provided to the experimental groups. These models were from the AlphaFold2 group, who overall submitted the most accurate predictions in CASP14. Four targets were solved with the aid of the models, and, additionally, the structure of an already solved target was improved. An a posteriori analysis showed that, in some cases, models from other groups would also be effective. This paper provides accounts of the successful application of models to structure determination, including molecular replacement for X-ray crystallography, backbone tracing and sequence positioning in a cryo-electron microscopy structure, and correction of local features. The results suggest that, in future, there will be greatly increased synergy between computational and experimental approaches to structure determination.


Assuntos
Biologia Computacional/métodos , Microscopia Crioeletrônica , Cristalografia por Raios X , Modelos Moleculares , Proteínas/química , Conformação Proteica , Software
7.
Mol Microbiol ; 108(1): 6-15, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29405518

RESUMO

Contractile tail bacteriophages, or myobacteriophages, use a sophisticated biomolecular structure to inject their genome into the bacterial host cell. This structure consists of a contractile sheath enveloping a rigid tube that is sharpened by a spike-shaped protein complex at its tip. The spike complex forms the centerpiece of a baseplate complex that terminates the sheath and the tube. The baseplate anchors the tail to the target cell membrane with the help of fibrous proteins emanating from it and triggers contraction of the sheath. The contracting sheath drives the tube with its spiky tip through the target cell membrane. Subsequently, the bacteriophage genome is injected through the tube. The structural transformation of the bacteriophage T4 baseplate upon binding to the host cell has been recently described in near-atomic detail. In this review we discuss structural elements and features of this mechanism that are likely to be conserved in all contractile injection systems (systems evolutionary and structurally related to contractile bacteriophage tails). These include the type VI secretion system (T6SS), which is used by bacteria to transfer effectors into other bacteria and into eukaryotic cells, and tailocins, a large family of contractile bacteriophage tail-like compounds that includes the P. aeruginosa R-type pyocins.


Assuntos
Bacteriófago T4/química , Bacteriófago T4/fisiologia , Proteínas da Cauda Viral/química , Proteínas da Cauda Viral/fisiologia , Bacteriófago T4/genética , Evolução Biológica , Membrana Celular/química , Membrana Celular/metabolismo , Genoma Viral , Bactérias Gram-Negativas/química , Bactérias Gram-Negativas/genética , Bactérias Gram-Negativas/fisiologia , Piocinas/química , Piocinas/metabolismo , Sistemas de Secreção Tipo VI/química , Sistemas de Secreção Tipo VI/genética , Sistemas de Secreção Tipo VI/fisiologia , Proteínas da Cauda Viral/genética , Difração de Raios X
8.
Nature ; 500(7462): 350-353, 2013 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-23925114

RESUMO

The bacterial type VI secretion system (T6SS) is a large multicomponent, dynamic macromolecular machine that has an important role in the ecology of many Gram-negative bacteria. T6SS is responsible for translocation of a wide range of toxic effector molecules, allowing predatory cells to kill both prokaryotic as well as eukaryotic prey cells. The T6SS organelle is functionally analogous to contractile tails of bacteriophages and is thought to attack cells by initially penetrating them with a trimeric protein complex called the VgrG spike. Neither the exact protein composition of the T6SS organelle nor the mechanisms of effector selection and delivery are known. Here we report that proteins from the PAAR (proline-alanine-alanine-arginine) repeat superfamily form a sharp conical extension on the VgrG spike, which is further involved in attaching effector domains to the spike. The crystal structures of two PAAR-repeat proteins bound to VgrG-like partners show that these proteins sharpen the tip of the T6SS spike complex. We demonstrate that PAAR proteins are essential for T6SS-mediated secretion and target cell killing by Vibrio cholerae and Acinetobacter baylyi. Our results indicate a new model of the T6SS organelle in which the VgrG-PAAR spike complex is decorated with multiple effectors that are delivered simultaneously into target cells in a single contraction-driven translocation event.


Assuntos
Proteínas de Bactérias/química , Proteínas de Bactérias/metabolismo , Sistemas de Secreção Bacterianos/genética , Repetições de Microssatélites/fisiologia , Acinetobacter/genética , Acinetobacter/metabolismo , Ligação Proteica , Vibrio cholerae/genética , Vibrio cholerae/metabolismo
9.
Mol Microbiol ; 105(3): 385-398, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28513100

RESUMO

Bacteriophages recognize and bind to their hosts with the help of receptor-binding proteins (RBPs) that emanate from the phage particle in the form of fibers or tailspikes. RBPs show a great variability in their shapes, sizes, and location on the particle. Some RBPs are known to depolymerize surface polysaccharides of the host while others show no enzymatic activity. Here we report that both RBPs of podovirus G7C - tailspikes gp63.1 and gp66 - are essential for infection of its natural host bacterium E. coli 4s that populates the equine intestinal tract. We characterize the structure and function of gp63.1 and show that unlike any previously described RPB, gp63.1 deacetylates surface polysaccharides of E. coli 4s leaving the backbone of the polysaccharide intact. We demonstrate that gp63.1 and gp66 form a stable complex, in which the N-terminal part of gp66 serves as an attachment site for gp63.1 and anchors the gp63.1-gp66 complex to the G7C tail. The esterase domain of gp63.1 as well as domains mediating the gp63.1-gp66 interaction is widespread among all three families of tailed bacteriophages.


Assuntos
Bacteriófago P22/fisiologia , Esterases/metabolismo , Adsorção/fisiologia , Animais , Bacteriófago P22/química , Bacteriófagos/fisiologia , Cristalografia por Raios X , Escherichia coli/metabolismo , Esterases/genética , Cavalos/microbiologia , Modelos Moleculares , Polissacarídeos Bacterianos/metabolismo , Ligação Proteica , Conformação Proteica , Proteínas da Cauda Viral/metabolismo
10.
Mol Cell ; 34(3): 375-86, 2009 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-19450535

RESUMO

The tailed bacteriophage phi29 has 12 "appendages" (gene product 12, gp12) attached to its neck region that participate in host cell recognition and entry. In the cell, monomeric gp12 undergoes proteolytic processing that releases the C-terminal domain during assembly into trimers. We report here crystal structures of the protein before and after catalytic processing and show that the C-terminal domain of gp12 is an "autochaperone" that aids trimerization. We also show that autocleavage of the C-terminal domain is a posttrimerization event that is followed by a unique ATP-dependent release. The posttranslationally modified N-terminal part has three domains that function to attach the appendages to the phage, digest the cell wall teichoic acids, and bind irreversibly to the host, respectively. Structural and sequence comparisons suggest that some eukaryotic and bacterial viruses as well as bacterial adhesins might have a similar maturation mechanism as is performed by phi29 gp12 for Bacillus subtilis.


Assuntos
Fagos Bacilares/ultraestrutura , Multimerização Proteica , Processamento de Proteína Pós-Traducional , Estrutura Quaternária de Proteína , Proteínas Estruturais Virais/química , Proteínas Estruturais Virais/metabolismo , Trifosfato de Adenosina/metabolismo , Sequência de Aminoácidos , Fagos Bacilares/química , Domínio Catalítico , Cristalografia por Raios X , Modelos Moleculares , Dados de Sequência Molecular , Alinhamento de Sequência , Proteínas Estruturais Virais/genética
11.
Environ Sci Technol ; 50(24): 13520-13528, 2016 12 20.
Artigo em Inglês | MEDLINE | ID: mdl-27709908

RESUMO

Common water disinfectants like chlorine have been reported to select for resistant viruses, yet little attention has been devoted to characterizing disinfection resistance. Here, we investigated the resistance of MS2 coliphage to inactivation by chlorine dioxide (ClO2). ClO2 inactivates MS2 by degrading its structural proteins, thereby disrupting the ability of MS2 to attach to and infect its host. ClO2-resistant virus populations emerged not only after repeated cycles of ClO2 disinfection followed by regrowth but also after dilution-regrowth cycles in the absence of ClO2. The resistant populations exhibited several fixed mutations which caused the substitution of ClO2-labile by ClO2-stable amino acids. On a phenotypic level, these mutations resulted in a more stable host binding during inactivation compared to the wild-type, thus resulting in a greater ability to maintain infectivity. This conclusion was supported by cryo-electron microscopy reconstruction of the virus particle, which demonstrated that most structural modification occurred in the putative A protein, an important binding factor. Resistance was specific to the inactivation mechanism of ClO2 and did not result in significant cross-resistance to genome-damaging disinfectants. Overall, our data indicate that resistant viruses may emerge even in the absence of ClO2 pressure but that they can be inactivated by other common disinfectants.


Assuntos
Compostos Clorados , Desinfecção , Cloro , Colífagos , Desinfetantes , Óxidos
12.
Mol Microbiol ; 92(1): 84-99, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24673724

RESUMO

Recognition of the bacterial host and attachment to its surface are two critical steps in phage infection. Here we report the identification of Gp108 as the host receptor-binding protein of the broad host-range, virulent Listeria phage A511. The ligands for Gp108 were found to be N-acetylglucosamine and rhamnose substituents of the wall teichoic acids of the bacterial cell wall. Transmission electron microscopy and immunogold-labelling allowed us to create a model of the A511 baseplate in which Gp108 forms emanating short tail fibres. Data obtained for related phages, such as Staphylococcus phages ISP and Twort, demonstrate the evolutionary conservation of baseplate components and receptor-binding proteins within the Spounavirinae subfamily, and contractile tail machineries in general. Our data reveal key elements in the infection process of large phages infecting Gram-positive bacteria and generate insights into the complex adsorption process of phage A511 to its bacterial host.


Assuntos
Proteínas de Bactérias/metabolismo , Listeria/virologia , Myoviridae/fisiologia , Proteínas Virais/metabolismo , Parede Celular/metabolismo , Genoma Viral , Microscopia Eletrônica de Transmissão , Myoviridae/classificação , Ramnose/metabolismo , Ácidos Teicoicos/metabolismo
14.
Nat Commun ; 15(1): 756, 2024 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-38272938

RESUMO

A contractile sheath and rigid tube assembly is a widespread apparatus used by bacteriophages, tailocins, and the bacterial type VI secretion system to penetrate cell membranes. In this mechanism, contraction of an external sheath powers the motion of an inner tube through the membrane. The structure, energetics, and mechanism of the machinery imply rigidity and straightness. The contractile tail of Agrobacterium tumefaciens bacteriophage Milano is flexible and bent to varying degrees, which sets it apart from other contractile tail-like systems. Here, we report structures of the Milano tail including the sheath-tube complex, baseplate, and putative receptor-binding proteins. The flexible-to-rigid transformation of the Milano tail upon contraction can be explained by unique electrostatic properties of the tail tube and sheath. All components of the Milano tail, including sheath subunits, are crosslinked by disulfides, some of which must be reduced for contraction to occur. The putative receptor-binding complex of Milano contains a tailspike, a tail fiber, and at least two small proteins that form a garland around the distal ends of the tailspikes and tail fibers. Despite being flagellotropic, Milano lacks thread-like tail filaments that can wrap around the flagellum, and is thus likely to employ a different binding mechanism.


Assuntos
Bacteriófagos , Sistemas de Secreção Tipo VI , Bacteriófagos/genética , Agrobacterium tumefaciens/genética , Sistemas de Secreção Tipo VI/metabolismo , Membrana Celular/metabolismo
15.
J Am Chem Soc ; 135(17): 6562-9, 2013 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-23560397

RESUMO

Bicyclic peptide ligands were found to have good binding affinity and target specificity. However, the method applied to generate bicyclic ligands based on phage-peptide alkylation is technically complex and limits its application to specialized laboratories. Herein, we report a method that involves a simpler and more robust procedure that additionally allows screening of structurally more diverse bicyclic peptide libraries. In brief, phage-encoded combinatorial peptide libraries of the format X(m)CX(n)CX(o)CX(p) are oxidized to connect two pairs of cysteines (C). This allows the generation of 3 × (m + n + o + p) different peptide topologies because the fourth cysteine can appear in any of the (m + n + o + p) randomized amino acid positions (X). Panning of such libraries enriched strongly peptides with four cysteines and yielded tight binders to protein targets. X-ray structure analysis revealed an important structural role of the disulfide bridges. In summary, the presented approach offers facile access to bicyclic peptide ligands with good binding affinities.


Assuntos
Compostos Bicíclicos com Pontes/química , Cisteína/química , Peptídeos/química , Alquilação , Sequência de Aminoácidos , Aminoácidos/química , Técnicas de Química Combinatória , Cristalização , Dissulfetos , Eletroforese em Gel de Poliacrilamida , Ligantes , Modelos Moleculares , Dados de Sequência Molecular , Oxirredução , Biblioteca de Peptídeos , Difração de Raios X
16.
EMBO J ; 28(7): 821-9, 2009 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-19229296

RESUMO

The contractile tail of bacteriophage T4 is a molecular machine that facilitates very high viral infection efficiency. Its major component is a tail sheath, which contracts during infection to less than half of its initial length. The sheath consists of 138 copies of the tail sheath protein, gene product (gp) 18, which surrounds the central non-contractile tail tube. The contraction of the sheath drives the tail tube through the outer membrane, creating a channel for the viral genome delivery. A crystal structure of about three quarters of gp18 has been determined and was fitted into cryo-electron microscopy reconstructions of the tail sheath before and after contraction. It was shown that during contraction, gp18 subunits slide over each other with no apparent change in their structure.


Assuntos
Bacteriófago T4/metabolismo , Proteínas da Cauda Viral/química , Clonagem Molecular , Microscopia Crioeletrônica , Cristalografia por Raios X , Escherichia coli/genética , Escherichia coli/metabolismo , Modelos Moleculares , Estrutura Terciária de Proteína , Proteínas da Cauda Viral/genética , Proteínas da Cauda Viral/isolamento & purificação
17.
Chembiochem ; 14(11): 1316-22, 2013 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-23828687

RESUMO

Improving the binding affinity and/or stability of peptide ligands often requires testing of large numbers of variants to identify beneficial mutations. Herein we propose a type of mutation that promises a high success rate. In a bicyclic peptide inhibitor of the cancer-related protease urokinase-type plasminogen activator (uPA), we observed a glycine residue that has a positive ϕ dihedral angle when bound to the target. We hypothesized that replacing it with a D-amino acid, which favors positive ϕ angles, could enhance the binding affinity and/or proteolytic resistance. Mutation of this specific glycine to D-serine in the bicyclic peptide indeed improved inhibitory activity (1.75-fold) and stability (fourfold). X-ray-structure analysis of the inhibitors in complex with uPA showed that the peptide backbone conformation was conserved. Analysis of known cyclic peptide ligands showed that glycine is one of the most frequent amino acids, and that glycines with positive ϕ angles are found in many protein-bound peptides. These results suggest that the glycine-to-D-amino acid mutagenesis strategy could be broadly applied.


Assuntos
Aminoácidos/metabolismo , Glicina/metabolismo , Peptídeos/metabolismo , Sítios de Ligação , Domínio Catalítico , Cristalografia por Raios X , Bases de Dados de Proteínas , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/química , Inibidores Enzimáticos/metabolismo , Humanos , Cinética , Ligantes , Mutagênese Sítio-Dirigida , Biblioteca de Peptídeos , Peptídeos/química , Peptídeos/genética , Ligação Proteica , Estabilidade Proteica , Proteólise , Especificidade por Substrato , Ativador de Plasminogênio Tipo Uroquinase/antagonistas & inibidores , Ativador de Plasminogênio Tipo Uroquinase/metabolismo
18.
J Virol ; 86(19): 10384-98, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22787233

RESUMO

Bacteriophage phi92 is a large, lytic myovirus isolated in 1983 from pathogenic Escherichia coli strains that carry a polysialic acid capsule. Here we report the genome organization of phi92, the cryoelectron microscopy reconstruction of its virion, and the reinvestigation of its host specificity. The genome consists of a linear, double-stranded 148,612-bp DNA sequence containing 248 potential open reading frames and 11 putative tRNA genes. Orthologs were found for 130 of the predicted proteins. Most of the virion proteins showed significant sequence similarities to proteins of myoviruses rv5 and PVP-SE1, indicating that phi92 is a new member of the novel genus of rv5-like phages. Reinvestigation of phi92 host specificity showed that the host range is not limited to polysialic acid-encapsulated Escherichia coli but includes most laboratory strains of Escherichia coli and many Salmonella strains. Structure analysis of the phi92 virion demonstrated the presence of four different types of tail fibers and/or tailspikes, which enable the phage to use attachment sites on encapsulated and nonencapsulated bacteria. With this report, we provide the first detailed description of a multivalent, multispecies phage armed with a host cell adsorption apparatus resembling a nanosized Swiss army knife. The genome, structure, and, in particular, the organization of the baseplate of phi92 demonstrate how a bacteriophage can evolve into a multi-pathogen-killing agent.


Assuntos
Bacteriófagos/genética , Bacteriófagos/metabolismo , Adsorção , Algoritmos , Biologia Computacional/métodos , Microscopia Crioeletrônica/métodos , Escherichia coli/metabolismo , Escherichia coli/virologia , Genoma , Genoma Bacteriano , Genômica , Especificidade de Hospedeiro , Modelos Genéticos , Conformação Molecular , Dados de Sequência Molecular , Fases de Leitura Aberta , RNA de Transferência/metabolismo , Salmonella/metabolismo , Salmonella/virologia , Análise de Sequência de DNA , Espectrometria de Massas em Tandem/métodos
19.
bioRxiv ; 2023 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-36865152

RESUMO

The contractile tail of bacteriophage P2 functions to drive the tail tube across the outer membrane of its host bacterium, a prerequisite event for subsequent translocation of phage genomic DNA into the host cell. The tube is equipped with a spike-shaped protein (product of P2 gene V , gpV or Spike) that contains a membrane-attacking Apex domain carrying a centrally positioned Fe ion. The ion is enclosed in a histidine cage that is formed by three symmetry-related copies of a conserved HxH (histidine, any residue, histidine) sequence motif. Here, we used solution biophysics and X-ray crystallography to characterize the structure and properties of Spike mutants in which the Apex domain was either deleted or its histidine cage was either destroyed or replaced with a hydrophobic core. We found that the Apex domain is not required for the folding of full-length gpV or its middle intertwined ß-helical domain. Furthermore, despite its high conservation, the Apex domain is dispensable for infection in laboratory conditions. Collectively, our results show that the diameter of the Spike but not the nature of its Apex domain determines the efficiency of infection, which further strengthens the earlier hypothesis of a drill bit-like function of the Spike in host envelope disruption.

20.
Commun Biol ; 6(1): 921, 2023 09 08.
Artigo em Inglês | MEDLINE | ID: mdl-37684529

RESUMO

Large gaps exist in our understanding of how bacteriophages, the most abundant biological entities on Earth, assemble and function. The structure of the "neck" region, where the DNA-filled capsid is connected to the host-recognizing tail remains poorly understood. We describe cryo-EM structures of the neck, the neck-capsid and neck-tail junctions, and capsid of the Agrobacterium phage Milano. The Milano neck 1 protein connects the 12-fold symmetrical neck to a 5-fold vertex of the icosahedral capsid. Comparison of Milano neck 1 homologs leads to four proposed classes, likely evolved from the simplest one in siphophages to more complex ones in myo- and podophages. Milano neck is surrounded by the atypical collar, which covalently crosslinks the tail sheath to neck 1. The Milano capsid is decorated with three types of proteins, a minor capsid protein (mCP) and two linking proteins crosslinking the mCP to the major capsid protein. The extensive network of disulfide bonds within and between neck, collar, capsid and tail provides an exceptional structural stability to Milano.


Assuntos
Bacteriófagos , Capsídeo , Proteínas do Capsídeo , Bacteriófagos/genética , Espinhas Dendríticas , Agrobacterium
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