Arsenic methylation capacity, body retention, and null genotypes of glutathione S-transferase M1 and T1 among current arsenic-exposed residents in Taiwan.

In order to elucidate the relationships among arsenic methylation capacity, body retention, and genetic polymorphisms of glutathione S-transferase (GST) M1 and T1, a total of 115 study subjects were recruited from Lanyang Basin located on the northeast coast of Taiwan. Specimens of drinking water, blood, urine, hair and toenail were collected from each study subject. Urinary inorganic and methylated arsenic were speciated by high performance liquid chromatography combined with hydride-generation atomic absorption spectrometry. Arsenic concentration in hair and toenail were quantitated by atomic absorption spectrophotometry. The polymerase chain reaction was used to determine genetic polymorphisms of GST M1 and T1. Arsenic concentrations in urine, hair, and toenail of study subjects were positively correlated with arsenic levels in their drinking water. Percentages of various arsenic species in urine (mean +/- standard error (SE) were 11.8 +/- 1.0, 26.9 +/- 1.2 and 61.3 +/- 1.4, respectively, for inorganic arsenic, monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA). Men and women had similar arsenic methylation capability. No associations were observed between arsenic methylation capability and arsenic content in either drinking water or urine. Ratios of arsenic contents in hair and toenail to urinary arsenic content (mean +/- standard error) were 6.2 +/- 0.7 and 16.5 +/- 1.7, respectively. Genetic polymorphisms of GST M1 and T1 were significantly associated with arsenic methylation. Subjects having the null genotype of GST M1 had an increased percentage of inorganic arsenic in urine, while those with null genotype of GST T1 had an elevated percentage of DMA in urine. Arsenic contents in hair and toenail were significantly correlated with the increase in arsenic concentrations of drinking water and urine, while no significant associations were observed between arsenic contents in hair and toenail and polymorphisms of GST M1 and T1. The relationship between arsenic methylation capability and body retention was modified by genetic polymorphisms of GST M1 and T1. Arsenic contents in hair and toenail were negatively associated with MMA percentage and positively associated with DMA percentage among subjects having null genotypes of GST M1 and T1, but not among those with non-null genotypes.

Congenital depression of the fetal skull: case report and review of literature.

Congenital skull depression in babies delivered by cesarean section (C/S) is an extremely rare occurrence and its etiology is frequently unknown. Such depression may be associated with brain injury and permanent cosmetic deformity. Surgical therapy is not the only choice since cranial CT is useful for identification of intracranial damage. In our case, CT scan revealed no intracranial injury and neurological examination of the baby was normal. Thereafter, the baby was managed expectantly and spontaneous resolution of the skull depression was noted by 14 weeks of age.

Estimation of single-root surface area from true thickness data and from thickness derived from digital dental radiography.

OBJECTIVES: The prognosis of a tooth affected by periodontitis may depend on the amount of bone remaining around the root surface. The aim of this study was to find how the surface area of a single root is related to the true thickness data and the calculated thickness data from digital dental radiographs. METHODS: Eight extracted single-root teeth were digitized three-dimensionally for direct surface area measurement. Meanwhile, they were also exposed to a digital dental X-ray system. The true thickness of the tooth root was measured. The estimated circumference data were calculated from both the measured thickness and the thickness estimated from the digital image and then measured and estimated circumferences were compared. RESULTS: (1) The largest circumference difference mean for measured thickness and for estimated thickness was -4.94% (+/-0.07%) and 23.02% (+/-1.12%) respectively. (2) The largest 95% confidence interval for difference means for measured thickness and for estimated thickness was (-2.82%, -1.87%) and (-5.42%, 2.22%), respectively. CONCLUSIONS: When the thickness data are available, the surface area of a single-root tooth can be estimated to an error of less than 5%. Theoretically, the root thickness can be derived from the projected radiological image of the tooth. However, the error of estimated circumference from digital dental radiography may be over 20%. This error can be minimized if the estimated thickness from digital dental radiography can be made more accurate.