RESUMO
Potato fruit juice (PFJ) is a by-product from industrial starch production. It still contains several valuable components such as amino acids, minerals and proteins. An economic technology for the isolation and purification of different native potato proteins is the ion exchange chromatography, which can be performed either by classical bed chromatography or by membrane adsorption chromatography (MA-IEX). An already published MA-IEX process for the downstreaming of PFJ is based on the following steps: prefiltration/microfiltration, fractionation with MA-IEX, ultra-/diafiltration and finally drying. In order to further minimize process complexity and costs, new MA-IEX-modules were designed and tested in this research project to facilitate the processing of crude, particle-containing solutions using a tangential flow through the membranes. Modules with fleece polymer spacers and extruded polymer spacers, as well as different spacer channel sizes were tested for their binding capacities and their long-term stability. An optimized setup was found for the technical scale. Modules with extruded polymer spacers channel size 250 µm show the highest binding capacities (anion exchanger approx. 0.34 mg/cm(2), cation exchanger approx. 0.16 mg/cm(2)), while the modules with extruded polymer spacers channel size 480 µm show the best long-term stability with 23 passes without intermediary cleaning.
Assuntos
Proteínas de Plantas/isolamento & purificação , Polímeros/química , Solanum tuberosum/química , Adsorção , Cromatografia , Frutas/química , Proteínas de Plantas/química , Amido/químicaRESUMO
During the symposium discussions were held in workshops on how the SAMS can support the transition of "molecular medicine" from research into practice. This transition is currently happening in prenatal diagnostics. With the help of molecular medicine doctors are at an early stage able to diagnose a variety of diseases, preliminary stages and dispositions of diseases--even with the unborn. Gene-diagnostics stands as a promising method of investigation, with no therapeutic equivalent yet. Here the SAMS should as soon as possible develop guidelines for the responsible use of gene-diagnostic methods; besides, the Academy should work towards connecting the use of gene-diagnostic methods to certain professional requirements. Furthermore, the SAMS is expected to ensure good basic and further training in the field of "molecular medicine" and to support "evidence-based medicine".
Assuntos
Genética Médica , Biologia Molecular/tendências , Pesquisa/tendências , Sociedades Médicas , Medicina Baseada em Evidências/tendências , Doenças Genéticas Inatas/diagnóstico , Doenças Genéticas Inatas/terapia , Genética Médica/normas , Genética Médica/tendências , Humanos , Garantia da Qualidade dos Cuidados de Saúde , Pesquisa/normas , SuíçaRESUMO
We have analyzed the mechanism of sex determination in the germ line of Drosophila by manipulating three parameters: (1) the ratio of X-chromosomes to sets of autosomes (X:A); (2) the state of activity of the gene Sex-lethal (Sxl), and (3) the sex of the gonadal soma. To this end, animals with a ratio of 2X:2A and 2X:3A were sexually transformed into pseudomales by mutations at the sex-determining genes Sxl (Sex-lethal), tra (transformer), tra-2 (transformer-2), or dsx (double-sex). Animals with the karyotype 2X;3A were also transformed into pseudofemales by the constitutive mutation SxlM1. The sexual phenotype of the gonads and of the germ cells was assessed by phase-contrast microscopy. Confirming the conclusions of Steinmann-Zwicky et al. (Cell 57, 157, 1989), we found that all three parameters affect sex determination in germ cells. In contrast to the soma in which sex determination is completely cell-autonomous, sex determination in the germ line has a non-autonomous component inasmuch as the sex of the soma can influence the sexual pathway of the germ cells. Somatic induction has a clear effect on 2X;2A germ cells that carry a Sxl+ allele. These cells, which form eggs in an ovary, can enter spermatogenesis in testes. Mutations that cause partial loss of function or gain of function of Sxl thwart somatic induction and, independently of the sex of the soma, dictate spermatogenesis or oogenesis, respectively. Somatic induction has a much weaker effect on 2X;3A germ cells. This ratio is essentially a male signal for germ cells which consistently enter spermatogenesis in testes, even when they carry SxlM1. In a female soma, however, SxlM1 enables the 2X;3A germ cells to form almost normal eggs. Our results show that sex determination in the germ line is more complex than in the soma. They provide further evidence that the state of Sxl, the key gene for sex determination and dosage compensation in the soma, also determines the sex of the germ cells, and that, in the germ line, the state of activity of Sxl is regulated not only by the X:A ratio, but also by somatic inductive stimuli.
Assuntos
Drosophila/genética , Gametogênese/fisiologia , Análise para Determinação do Sexo , Animais , Feminino , Gametogênese/genética , Regulação da Expressão Gênica/genética , Genótipo , Células Germinativas/fisiologia , Camadas Germinativas/fisiologia , Gônadas/fisiologia , Masculino , Mutação , FenótipoRESUMO
Different human carcinoma cell lines were screened for the presence of Ca2(+)-binding oncomodulin. A specific polyclonal antibody was raised against a synthetic peptide (amino acids 99-108) of oncomodulin coupled to hemocyanin. Extracts of tumor cell lines (several human, one rat) were analyzed for the presence of oncomodulin by immunoblotting. A strong immunoreaction of oncomodulin was obtained in chemically transformed rat fibroblasts (T14c) in contrast to all human tumor cell lines investigated, where no immunoreaction was obtained. These results suggest that oncomodulin cannot be used in diagnostics of human tumors.
Assuntos
Proteínas de Ligação ao Cálcio/metabolismo , Carcinoma/metabolismo , Proteínas de Neoplasias/metabolismo , Humanos , Técnicas Imunológicas , Técnicas In Vitro , Proteínas de Neoplasias/imunologia , Fragmentos de Peptídeos/imunologia , Células Tumorais CultivadasRESUMO
The pleural human mesothelioma cell line ZL5 established in our laboratory exhibits an unusual phenotype with adherent and floating cells. ZL5 cells grow in a chemically defined medium (ACL3*) and can be maintained over 3 weeks in protein-free basal medium alone (RPMI). Basal medium conditioned by ZL5 cells possesses a mitogenic activity with an autocrine effect, as measured by cell counting and by a 3H-thymidine incorporation assay. Moreover, the conditioned medium affects the DNA synthesis of a variety of other lung-derived cells. The active principle of medium conditioned by ZL5 cells is not identical to the defined growth-factors EGF, PDGF, and TGF-beta, known to stimulate the growth of normal human mesothelial cells: treatment with these factors does not mimic the effect of conditioned medium on ZL5 cells. Our observations suggest that the mesothelioma cell line ZL5 produces an unknown autocrine mitogen.
Assuntos
Mesotelioma/patologia , Mitógenos/metabolismo , Adenocarcinoma , Adulto , Animais , Carcinoma de Células Escamosas , Divisão Celular , Linhagem Celular , Bandeamento Cromossômico , Meios de Cultura , Replicação do DNA , Humanos , Cariotipagem , Pulmão , Masculino , Mesotelioma/genética , Mesotelioma/metabolismo , Camundongos , Camundongos Nus , Mitógenos/isolamento & purificação , Transplante de Neoplasias , Derrame Pleural/patologia , Transplante Heterólogo , Células Tumorais CultivadasRESUMO
Epidermal growth factor and its receptor (EGFR) constitute an important and well-characterized mitogenic system in various ectodermal tissues including glial cells. Over-expression of the EGFR due to gene amplification has been reported in primary brain tumours of glial origin. Using a monoclonal antibody to the EGFR and immunohistochemical analysis, we examined the expression and distribution of EGFR in 103 astrocytic tumours. In addition, selected tumours were studied by Western blotting using a polyclonal antibody to EGFR and by Southern blot analysis. Glioblastomas (WHO grade IV) showed EGFR expression in 37% of cases, whereas pilocytic (WHO grade I), low-grade (WHO grade II) or anaplastic astrocytoma (WHO grade III) were invariably EGFR negative. Generally, there was a close correlation between the presence of EGFR gene amplification and over-expression of receptor protein. Different patterns of immunoreactive cells and significant intratumour heterogeneity of EGFR expression were observed in glioblastomas. The specific association of EGFR over-expression with glioblastoma may provide a useful diagnostic tool for distinguishing anaplastic astrocytoma (WHO grade III) and glioblastoma multiforme (WHO grade IV).