RESUMO
In this paper we report the discovery of bacteriolytic activity of an immune system cytokine mediator, interleukin-2. Bacteriolytic activity of interleukin-2 was compared with a well-known bacteriolytic enzyme - chicken egg white lysozyme - by monitoring the lysis of the Gram-negative bacterium Escherichia coli, the Gram-positive coccus Micrococcus luteus, and the Gram-positive spore-forming bacillus Bacillus subtilis. It was found that interleukin-2 has greater specificity to the Gram-negative bacterium E. coli than does lysozyme. In contrast to chicken egg white lysozyme, interleukin-2 does not lyse the Gram-positive coccus M. luteus and the Gram-positive spore-forming bacillus B. subtilis. These results give a new understanding of the biological functions of interleukin-2, a regulatory protein that plays a role in oncological and infectious diseases.
Assuntos
Interleucina-2/metabolismo , Bacillus subtilis/metabolismo , Bacteriólise , Escherichia coli/metabolismo , Humanos , Micrococcus luteus/metabolismo , Muramidase/metabolismoRESUMO
In the present work the studies ofbacteriolytic factors from sheep blood plasma have been performed. Three novel enzymes have been identified and characterized. Two of them have a molecular weight 15 +/- 2 kDa and able to lyse the gram-negative Escherichia coli bacteria. The third enzyme has a molecular weight 34 +/- 4 kDa and is able to lyse both gram-negative Escherichia coli and gram-positive Micrococcus luteus bacteria. The bacteriolytic reactions have been studied for all three enzymes; particularly, pH-optima have been identified with respect to the substrate. To identify the enzymes trypsinolysis and consequent MALDI-TOF mass spectrometry studies were performed. The results were compared to data from publicly available databases, such as Swiss-Prot, NCBI, MSDB.
Assuntos
Antibacterianos/química , Bacteriólise , Muramidase/química , Peptídeo Hidrolases/química , Plasma/enzimologia , Ovinos/sangue , Animais , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Bacillus subtilis/efeitos dos fármacos , Parede Celular/química , Cromatografia em Gel , Cromatografia por Troca Iônica , Bases de Dados Factuais , Escherichia coli/efeitos dos fármacos , Micrococcus luteus/efeitos dos fármacos , Peso Molecular , Muramidase/isolamento & purificação , Muramidase/farmacologia , Peptídeo Hidrolases/isolamento & purificação , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por MatrizRESUMO
Bacteriophage enzyme preparations exolysin and endolysin were studied. Exolysin (a phage-associated enzyme) was obtained from tail fraction and endolysin from phage-free cytoplasmic fraction of disintegrated Salmonella enteritidis cells. A new method for purification of these enzymes was developed, and their molecular masses were determined. The main catalytic properties of the studied enzymes (pH optimum and specificity to bacterial substrates) were found to be similar. Both enzymes lyse Escherichia coli cells like chicken egg lysozyme, but more efficiently lyse S. enteritidis cells and cannot lyse Micrococcus luteus, a good substrate for chicken egg lysozyme. Similar properties of exolysin and endolysin suggest that these enzymes are structurally similar or even identical.
Assuntos
Endopeptidases/química , Fagos de Salmonella/enzimologia , Proteínas Virais/química , Animais , Biocatálise , Galinhas , Endopeptidases/isolamento & purificação , Endopeptidases/metabolismo , Escherichia coli/metabolismo , Muramidase/metabolismo , Salmonella enteritidis/efeitos dos fármacos , Especificidade por Substrato , Proteínas Virais/isolamento & purificação , Proteínas Virais/metabolismoRESUMO
To simulate in vitro the conditions under which enzymes act in vivo, enzyme molecules have been entrapped in hydrated reverse micelles of a surfactant in organic solvents. In this system the catalytic activity of one of the enzymes studied (peroxidase) became much higher than in water, and the specificity of the other enzyme (alcohol dehydrogenase) was dramatically altered.
Assuntos
Catálise , Enzimas/metabolismo , Água , Oxirredutases do Álcool/metabolismo , Cinética , Micelas , Peroxidases/metabolismo , SolventesRESUMO
The influence of biomembrane lipids on the catalytic activity of a peripheral membrane enzyme, acid phosphatase (AP), was studied in a reverse micellar system. It was found that the interaction of AP with lipids led to a number of kinetic effects depending on lipid nature on enzyme function. The observed effects might be caused by the formation of lipoprotein complexes as well as by the influence of lipids on structure and properties of the micellar matrix. The results are important for clear understanding of molecular mechanisms of regulation of the catalytic activity of the membrane-associated enzyme in vivo. These data can also be used as a physicochemical basis for application of AP in medical fields as a diagnostic tool for diseases caused by changes in lipid metabolism, e.g. urinary, orthopedic, and allergic diseases.
Assuntos
Fosfatase Ácida/química , Lipídeos/química , Micelas , Proteínas de Plantas/química , Fosfatase Ácida/metabolismo , Algoritmos , Anisotropia , Catálise/efeitos dos fármacos , Dicroísmo Circular , Cinética , Lipídeos/farmacologia , Lipoproteínas/química , Lipoproteínas/metabolismo , Proteínas de Plantas/metabolismo , Tensoativos/química , Tensoativos/metabolismo , Triticum/enzimologiaRESUMO
An effective approach to the stabilization of hydrolytic enzymes (alkaline proteinase and cellulases) via the complex formation with chitosan for their further use as detergent components has been developed. Interaction with chitosan results in a 35-50% increase in the level of catalytic activity of the enzymes after incubation for 60 min under the conditions of detergent use (alkaline pH, increased temperature, the presence of anionic surfactants) as compared to the system in the absence of chitosan both due to the enzyme stabilization and the increase of the starting level of catalytic activity. A twofold decrease of the enzyme inactivation constant is observed under the aforementioned conditions in the case of alkaline proteinase. In the case of cellulase preparation, the method for the control of the concentration of the active enzyme in the system modeling synthetic detergents has been suggested. The method is based on the enzymatic destruction of the stabilizing agent, chitosan, by enzymes of the cellulase complex. The destruction of chitosan removed the stabilizing effect, thus resulting in the inactivation of cellulases. The developed approaches allow for the widening of the field of the possible application of enzymes as detergent components.
Assuntos
Proteínas de Bactérias/química , Celulase/química , Quitosana/química , Detergentes/química , Endopeptidases/química , Estabilidade Enzimática , Temperatura Alta , Concentração de Íons de HidrogênioRESUMO
The effect of various compounds on the activity and stability of a phage-associated enzyme lysing cells of streptococci of groups A and C (PlyC) was investigated. Substantial inhibition of the enzyme activity was revealed at an increased ionic strength (in the presence of NaCl) and upon the addition of carbohydrates (mono-, di-, and polysaccharides), i.e., agents stabilizing many enzymes. It was established that the enzyme activity was substantially reduced in the presence of positively charged polyelectrolytes and surfactants, whereas incubation with micelle-forming substances and negatively charged polyelectrolytes led to PlyC activation and stabilization. It was shown that, in the mycelial polyelectrolyte composition M16, the enzyme retained its activity for 2 months; while in a buffer solution under the same conditions (pH 6.3, room temperature), it practically completely lost its activity in 2 days. Characteristics of the enzyme thermal inactivation were found, in particular, its semiinactivation time at various temperatures; these allowed us to estimate its behavior at any temperature and to recommend conditions for its storage and use.
Assuntos
Bacteriófagos/enzimologia , Streptococcus pyogenes/efeitos dos fármacos , Proteínas Virais/farmacologia , Contagem de Colônia Microbiana , Estabilidade Enzimática , Concentração de Íons de Hidrogênio , Micelas , Nefelometria e Turbidimetria , Concentração Osmolar , Infecções Estreptocócicas/tratamento farmacológico , Infecções Estreptocócicas/prevenção & controle , Tensoativos , Temperatura , Fatores de Tempo , Proteínas Virais/químicaRESUMO
The bacteriolytic activity of interleukin-2 and chicken egg lysozyme in the presence of various substances has been studied. Glycine and lysine do not affect the activity of interleukin-2 but increase that of lysozyme, showing a bell-shape concentration dependence peaking at 1.5 mM glycine and 18 mM lysine. Arginine and glutamate activate both interleukin-2 and lysozyme with a concentration dependence of the saturation type. Aromatic amino acids have almost no effect on the activity of both interleukin-2 and lysozyme. Aromatic amines, tryptamine, and tyramine activate interleukin-2 but inhibit lysozyme. Peptide antibiotics affect interleukin and lysozyme similarly and exhibit maximum activity in the micromolar range of antibiotics. Taurine has no effect on the activity of interleukin-2 and lysozyme. Mildronate showed no influence on lysozyme, but it activated interleukin-2 with the activity maximum at 3 mM. EDTA activates both interleukin-2 and lysozyme at concentrations above 0.15 mM.
RESUMO
The bacteriolytic activity of interleukin-2 and hen egg white lysozyme against 34 different species of microorganisms has been studied. It was found that 6 species of microorganisms are lysed in the presence of interleukin-2. All interleukin-2-sensitive microorganisms belong either to the Enterobacteriaceae, Bacillaceae, or the Lactobacillaceae family. It was also found that 12 species of microorganisms are lysed in the presence of lysozyme, and 16 species of microorganisms are lysed in the presence of sodium dodecyl sulfate (SDS). The bacteriolytic activity of interleukin-2 and lysozyme was studied at various pH values.
RESUMO
Regulation of the supramolecular organization and the catalytic activity of GM1-galactosidase (EC 3.2.1.23) and neuraminidase (EC 3.2.1.18) from human kidney was studied in a system of hydrated reversed micelles of Aerosol OT in octane. It was shown that both the catalytic activity and the oligomeric structure of the GM1-galactosidase in reversed micelles depend on the [H2O]/[Aerosol OT] molar ratio (w(o)). GM1-galactosidase 64-67 kDa monomers, 260 kDa tetramers, and 660 kDa octamers were obtained in systems with w(o) = 0-20, 25-30 and 30-40, respectively. The association of GM1-galactosidase monomers into an octamer results in the cooperative increase in enzymatic activity. 'Protective protein', a component of the GM1-galactosidase-neuraminidase native complex, was found to improve this association significantly.
Assuntos
Neuraminidase/química , beta-Galactosidase/química , Glicolipídeos/química , Humanos , Rim/enzimologia , Lisossomos/enzimologia , Micelas , Neuraminidase/isolamento & purificação , Octanos , Conformação Proteica , beta-Galactosidase/isolamento & purificaçãoRESUMO
Micellar enzymology, a new trend in molecular biology, studies catalysis by enzymes entrapped in hydrated reversed micelles composed of surfactants (phospholipids, detergents) in organic solvents. The key research problems of micellar enzymology and its relation to enzyme membranology are discussed.
Assuntos
Enzimas/fisiologia , Técnicas In Vitro , Cinética , Substâncias Macromoleculares , Membranas/enzimologia , Micelas , Solubilidade , Especificidade por Substrato , Termodinâmica , ÁguaRESUMO
1. The possibility of stabilizing water-soluble enzymes against the inactivation action of organic solvents by means of surfactants has been studied. Several enzymes (alpha-chymotrypsin (EC 3.4.21.1), trypsin (EC 3.4.21.4), pyrophosphatase (EC 3.6.1.1), peroxidase (EC 1.11.1.7), lactate dehydrogenase (EC 1.1.1.27) and pyruvate kinase (EC 2.7.1.40)) were used to demonstrate that enzymes can be entrapped into reversed micelles formed by surfactants (Aerosol OT, cetyltrimethylammonium bromide, Brij 56) in an organic solvent (benzene, chloroform, octane, cyclohexane). The enzymes solubilized in this way retain their catalytic activity and substrate specificity. 2. A kinetic theory has been put forward that describes enzymatic reactions occurring in a micelle-solvent pseudobiphasic system. In terms of this theory, an explanation is given for the experimental dependence of the Michaelis-Menten equation parameters on the concentrations of the components of a medium (water, organic solvent, surfactant) and also on the combination of the signs of charges in the substrate molecule and on interphase (++, +-, --). 3. The results obtained by us may prove important for applications of enzymes in organic synthesis and for studying the state and role of water in the structure of biomembranes and active centres of enzymes.
Assuntos
Coloides , Enzimas/metabolismo , Micelas , Solventes/farmacologia , Tensoativos/farmacologia , Benzeno/farmacologia , Inibidores Enzimáticos , Cinética , Modelos Químicos , ÁguaRESUMO
Structure and dynamic properties of free poly(methacrylic acid) (PMA) and PMA complexed with alpha-chymotrypsin (CT) were studied using the time resolved fluorescence anisotropy technique. We have found that the interaction of PMA with CT induces the formation of a quasi-regular structure of PMA. At a CT/PMA weight ratio of 4:1 the interaction with CT leads to formation of approximately four equal segments of polyelectrolyte, each binding one CT molecule and characterized by an independent rotational mobility. Increase of the CT/PMA weight ratio above 8:1 gives rise to the overall rotation of the whole enzyme-polyelectrolyte complex. In water-ethanol mixtures the mobility of PMA segments containing CT decreases and the structure of the complex becomes even more rigid due to enhancement of the electrostatic interaction between CT and PMA. Formation of the compact and quasi-regular structure of the complex is perhaps the main reason behind the enhancement of enzyme stability and suppression of enzyme aggregation in water-organic cosolvent mixtures.
Assuntos
Quimotripsina/química , Ácidos Polimetacrílicos/química , Animais , Sítios de Ligação , Bovinos , Dicroísmo Circular , Estabilidade Enzimática , Etanol/química , Polarização de Fluorescência , Isoxazóis/química , Estrutura Molecular , Conformação Proteica , Pirenos/química , Solventes/químicaRESUMO
A heterodimeric enzyme (gamma-glutamyltransferase) was studied in the reversed micellar medium of Aerosol OT (AOT) in octane. As was shown earlier, the size (radius) of inner cavity of the AOT-reversed micelles is determined by their hydration degree, i.e., [H2O]/[AOT] molar ratio, in the system. Owing to this, the dependence of hydrolytic, transpeptidation and autotranspeptidation activities of the enzyme on the hydration degree was investigated using L- and D-isomers of gamma-glutamyl(3-carboxy-4-nitro)anilide and glycylglycine as substrates. For all of the reaction types, the observed dependences are curves with three optima. The optima are found at the hydration degrees, [H2O]/[AOT] = 11, 17 and 26 when the inner cavity radii of reversed micelles are equal to the size of light (Mr 21,000) and heavy (Mr 54,000) subunits of gamma-glutamyltransferase, and to their dimer (Mr 75,000), respectively. Ultracentrifugation experiments showed that a change of the hydration degree resulted in a reversible dissociation of the enzyme to light and heavy subunits. The separation of light and heavy subunits of gamma-glutamyltransferase formed in reversed micelles was carried out and their catalytic properties were studied. The two subunits catalyze hydrolysis and transpeptidation reactions; autotranspeptidation reaction is detected only in the case of the heavy subunit. These findings imply that the reversed micelles of surfactants in organic solvents function as the matrices with adjustable size permitting to regulate the supramolecular structure and the catalytic activity of oligomeric enzymes.
Assuntos
Coloides , Micelas , gama-Glutamiltransferase/metabolismo , Catálise , Centrifugação , Ácido Dioctil Sulfossuccínico , Modelos Químicos , Complexos Multienzimáticos , Octanos , Solventes , Tensoativos , gama-Glutamiltransferase/análiseRESUMO
Hydrophilized and hydrophobized forms of the lipase from Mucor miehei were obtained by its chemical modification with cellobiose and N-hydroxysuccinimidyl palmitate with a modification degree of 4 in both cases. A comparative analysis of the regulation of the catalytic activities of the native and modified lipases was carried out in the system of reversed micelles of OT aerosol (AOT) in isooctane. The level of catalytic activity of all the lipase preparations in the micellar medium was found to be higher than that in aqueous solution. The chemical modification of lipase did not result in a change in the regulation of the oligomeric composition of the enzyme controlled by the degree of micelle hydration omega0 (micelle size). The kcat dependences on omega0 for each lipase preparation exhibit two maxima, corresponding to the functioning of lipase monomers and tetramers. The changes in the hydrophilic-lipophilic balance of the lipase surface significantly affect the character of the regulation of enzyme activity due to changes in the surfactant concentration (the number of micelles). The lipase hydrophobization results in a decrease in the enzyme activation effect with an increase in the AOT concentration in comparison with the native lipase. The lipase hydrophilization dramatically decreases the activity of lipase tetramer when the AOT concentration is increased. The catalytic activity of the monomer of hydrophilized lipase is practically independent of the AOT concentration. Kinetic data indicate a mixed type of activation of both oligomeric forms of the native and the hydrophobized lipase by AOT molecules and the noncompetitive type of the activation and AOT inhibition of the monomer and the tetramer of the hydrophilized lipase, respectively. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2005, vol. 31, no. 6; see also http://www.maik.ru.
Assuntos
Celobiose/química , Ácido Dioctil Sulfossuccínico , Lipase/química , Micelas , Palmitatos/química , Ácidos Palmíticos/química , Succinimidas/química , Catálise , Interações Hidrofóbicas e Hidrofílicas , Mucor/enzimologia , OctanosRESUMO
Using reversed micelles of surfactants solvated by water-organic cosolvent mixtures as a matrix for enzyme entrapping, it is possible to fix the highly reactive alpha-chymotrypsin form. The reactivity of alpha-chymotrypsin towards nonspecific substrates increases to the extent comparable with that observed in reactions involving specific substrates.
Assuntos
Quimotripsina , Animais , Bovinos , Quimotripsina/metabolismo , Técnicas In Vitro , Cinética , Micelas , Movimento (Física) , Pâncreas/enzimologia , Conformação Proteica , Solventes , Relação Estrutura-AtividadeRESUMO
A comparative study of native and artificially glycosylated alpha-chymotrypsin in reversed micelles of Aerosol OT in octane was carried out. D-Glucosamine and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide were used as modifying agents to yield glycosylated enzyme. Unlike the native alpha-chymotrypsin, the modified protein tended to form reversible oligomeric structures, revealed by the appearance of an additional maximum (characteristic of dimeric forms of protein functioning) as a result of the enzyme catalytic activity being dependent on the AOT hydration degree. Dependence of the enzyme catalytic activity on the surfactant concentration in the case of the modified enzyme was similar to that of glycoproteins, and suggests its membrane affinity. The role of carbohydrate moieties in the functioning of glycoproteins is discussed.
Assuntos
Quimotripsina/química , Quimotripsina/metabolismo , Glicoproteínas/química , Glicoproteínas/metabolismo , Animais , Bovinos , Glucosamina , Glicosilação , Indicadores e Reagentes , Cinética , Substâncias Macromoleculares , Pâncreas/enzimologiaRESUMO
The regularities of their functioning of enzyme, water-soluble and membrane forms, in the systems of the reversed micelles of surfactants in organic solvents are compared. Using as examples gamma-glutamyltransferase (in AOT reversed micelles in octane) and aminopeptidase (in Brij 96 reversed micelles in cyclohexane), the principal difference in the catalytic activity regulation of water-soluble and membrane forms is demonstrated. The catalytic activity of the membrane form depends considerably on the surfactant concentration at the constant degree of hydration, whereas the activity of the water-soluble form is constant under these conditions. The catalytic activity dependence on the surfactant concentration is regarded as a test for enzyme membrane activity.
Assuntos
Aminopeptidases/análise , Membrana Celular/enzimologia , gama-Glutamiltransferase/análise , Animais , Encéfalo/enzimologia , Carcinoma Hepatocelular/enzimologia , Catálise , Bovinos , Cicloexanos , Neoplasias Hepáticas , Micelas , Octanos , Solubilidade , ÁguaRESUMO
The stability of alpha-chymotrypsin covalently modified with a strongly hydrophilic modifier, pyromellitic dianhydride, against solvent-induced denaturation in water-organic solvent binary mixtures has been studied. It was found that the hydrophilization results in a strong stabilization of the enzyme against denaturation by organic solvents. The stabilizing effect is explained in terms of the enhanced ability of the hydrophilized enzyme to keep its hydration shell, which is indispensable for supporting the native protein conformation, from denaturing stripping by organic solvents.
Assuntos
Quimotripsina/química , Solventes , Benzoatos/farmacologia , Fenômenos Químicos , Físico-Química , Estabilidade de Medicamentos , Metanol/farmacologia , Estrutura Molecular , Desnaturação Proteica , Soluções , Termodinâmica , ÁguaRESUMO
The phenomenon of regulation of the catalytic activity of enzymes via changing their oligomeric composition in the system of reversed micelles of sodium bis(2-ethylhexyl)sulfosuccinate (AOT) in octane was studied using alpha-chymotrypsin (CT) from bovine brain and alkaline phosphatase (AP) from calf intestinal mucosa. The dependences of the enzyme catalytic activity on the AOT hydration degree (Wo = [H2O]/[AOT]), the parameter determining the radius (rc) of the inner cavity of micelles, usually represent the bell-shaped curves. The maximal catalytic activity is observed at such Wo when rc is equal to the size of the enzyme molecule. The position of this maximum strictly correlates with the enzyme oligomeric composition. Thus, in the case of CT this is observed at Wo = 12 when rc is equal to the radius (rp) of the CT globule. In the case of artificially produced conjugate containing six cross-linked CT molecules, this is observed at Wo = 43 when rc is equal to the radius of the sphere surrounding the absolute octahedron composed of six CT globules. The dependence of the catalytic activity of AP on Wo represents a curve with two maxima that are observed when rc is equal to rp of either AP monomer (Wo = 17) or AP dimer (Wo = 25). Ultracentrifugation experiments revealed that variation of Wo causes a change in the oligomeric composition of AP - its transition from monomeric (Wo less than 20) to dimeric form (Wo greater than 20). Hence, the observed maxima correspond to functioning of different oligomeric forms of AP.