A strategy of co-fermentation of distillers dried grains with solubles (DDGS) and lignocellulosic feedstocks as swine feed.

To meet the sustainable development of the swine feed industry, it is essential to find alternative feed resources and develop new feed processing technologies. Distillers dried grains with solubles (DDGS) is a by-product from the ethanol industry consisting of adequate nutrients for swine and is an excellent choice for the swine farming industry. Here, a strategy of co-fermentation of DDGS and lignocellulosic feedstocks for production of swine feed was discussed. The potential of the DDGS and lignocellulosic feedstocks as feedstock for fermented pig feed and the complementary relationship between them were described. In order to facilitate the swine feed research in co-fermentation of DDGS and lignocellulosic feedstocks, the relevant studies on strain selection, fermentation conditions, targeted metabolism, product nutrition, as well as the growth and health of swine were collected and critically reviewed. This review proposed an approach for the production of easily digestible and highly nutritious swine feed via co-fermentation of DDGS and lignocellulosic feedstocks, which could provide a guide for cleaner swine farming, relieve stress on the increasing demand of high-value swine feed, and finally support the ever-increasing demand of the pork market.

Insights into antibiotic resistance gene abundances and regulatory mechanisms induced by ionic liquids during composting.

This study investigated the regulatory mechanism of the evolution of antibiotic resistance genes (ARGs) during the composting process with sawdust and cow manure as raw materials using ionic liquids (ILs) pretreatment. The results showed that genes of MLS, chloramphenicol, tetracycline, beta - lactam as composting gradually decreased. From day0 to day3, MLS in control group (CK) and experimental group (T) decreased by 25.62% and 26.66%, respectively. Tetracycline decreased by 7.21% in CK and by 7.86% in T. Chloramphenicol decreased by 2.85% in CK and 3.34% in T. Beta-lactam decreased by 1.95% in Ck and by 3.69% in T. Mechanism studies have shown that ILs can effectively decompose extracellular polymeric substances (EPS) and enhance lactose dehydrogenase (LDH) release, resulting in ARGs release and elimination. Meanwhile, ILs pretreatment can inhibit growth of some ARGs hosts, especially Firmicutes, resulting in decreased ARGs. Moreover, metabolic pathways and related genes take part in ARGs transmission were down regulated, leading to decreased ARGs.

The fates of antibiotic resistance genes and their association with cell membrane permeability in response to peroxydisulfate during composting.

The aims of this study were to use metagenomics to reveal the fates of antibiotic resistance genes (ARGs) during composting under the regulation of peroxydisulfate and clarify the relationship between ARGs and cell membrane permeability. Results showed that peroxydisulfate increased cell membrane permeability by effectively regulating the expression of outer membrane protein and lipopolysaccharide related genes. Besides, it reduced polysaccharides and proteins in extracellular polymer substances by 36% and 58%, respectively, making it easier for intracellular ARGs (i-ARGs) to reach the extracellular environment, among which the absolute intracellular abundance of mphK, Erm(31), and tet(44) decreased to 1.2, 1.0, and 0.89 fold of the control, respectively. Finally, variation partitioning analysis showed that i-ARGs dominated the removal of ARGs. These results revealed that the removal of i-ARGs by activated peroxydisulfate was the key to the removal of ARGs and increased cell membrane permeability played a key role for peroxydisulfate to remove i-ARGs during composting.

Mechanism analysis of humification coupling metabolic pathways based on cow dung composting with ionic liquids.

This study focused on how adding ionic liquids (IL) affects composting humification. During the warming and thermophilic phases, addition of IL increased precursors content, and increased the polymerization of humus (HS) at later stages. Furthermore, the final HS and humic acid (HA) content of experimental groups (T) groups 129.79 mg/g and 79.91 mg/g were higher than in control group (CK) 118.57 mg/g and 74.53 mg/g, respectively (p < 0.05). IL up-regulated the gene abundance of metabolism for carbohydrate and amino acid (AA), and promoted the contributions of Actinobacteria and Proteobacteria, which affected humification. The redundancy analysis (RDA) results showed that the citrate-cycle (TCA cycle)(ko0020), pentose phosphate pathway (ko00030), pyruvate metabolism (ko00620), glyoxylate and dicarboxylate metabolism (ko00630), propanoate metabolism (ko00640), butanoate metabolism (ko00650) positively correlated with HA and HI. HA and humification index (HI) positively correlated with AA metabolic pathways, and fulvic acid (FA) was negatively correlated with these pathways. Overall, metabolism for carbohydrate and AA metabolism favored compost humification. ILs improved metabolism for carbohydrate and amino acid metabolism, thus enhancing humification.

Performances of antibiotic resistance genes profile upon the action of biochar-activated peroxydisulfate in composting.

In this study, the amendment of biochar-activated peroxydisulfate during composting to remove antibiotic resistance genes (ARGs) by direct (microbial community succession) and indirect methods (physicochemical factors) was analyzed. When implementing indirect methods, the synergistic effect of peroxydisulfate with biochar optimized the physicochemical habitat of compost, maintaining its moisture within a range of 62.95%-65.71%, and a pH of 6.87-7.73, and causing the compost to mature 18 days earlier than the control groups. The direct methods caused the optimized physicochemical habitat to adjust the microbial communities and reduce the abundance of most of the ARG host bacteria (Thermopolyspora, Thermobifida, and Saccharomonospora), thus inhibiting this substance's amplification. Heatmap analysis confirmed the necessary connection between physicochemical factors, microbial communities, and ARGs. Moreover, a mantel test confirmed the direct significant effect of the microbial communities on ARGs and the indirect significant effect of physicochemical factors on ARGs. The results showed that the abundance of more ARGs was down-regulated at the end of composting and regulated by biochar-activated peroxydisulfate, especially for the abundance of AbaF, tet(44), golS, and mryA, which was significantly decreased by 0.87-1.07 fold. These results provide new insights into the removal of ARGs during composting.

Metagenomics analysis unraveled the influence of sulfate radical-mediated compost nitrogen transformation process.

The mechanism of nitrogen transformation of sulfate radical (SO- 4⋅) in the process of composting is unclear. The objectives of this study were to investigate the influence of SO- 4⋅ on nitrogen biotransformation during composting and to compare the differences in physicochemical parameters and metagenomics analysis between CK (fresh dairy manure and bagasse pith) and PS (the composting raw materials added with potassium persulfate). The results indicated that SO-4⋅ guides electron transfer in the conversion of NH+4-N to NO- 3-N and breaches the extracellular polysaccharide (EPS) structure to promote nitrogen removal. Aminomonooxygenase (AMO) and nitrate reductase (NR) levels displayed an interactive relationship between microorganisms and substrates. Metagenomics analysis revealed distinct microbial community compositions and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways between nitrification and denitrification. Correlation analysis indicated that Methanobrevibacter, Bacillus and Pseudomonas were closely related to these processes. This work demonstrates the effect of SO- 4⋅ on nitrogen cycling and retention, and possible mechanisms of nitrification and denitrification during composting.

Biochar-based solid acid accelerated carbon conversion by increasing the abundance of thermophilic bacteria in the cow manure composting process.

This study investigated the effects of biochar-based solid acids (SAs) on carbon conversion, alpha diversity and bacterial community succession during cow manure composting with the goal of providing a new strategy for rapid carbon conversion during composting. The addition of SA prolonged the thermophilic phase and accelerated the degradation of lignocellulose; in particular, the degradation time of cellulose was shortened by 50% and the humus content was increased by 22.56% compared with the control group (CK). In addition, high-throughput sequencing results showed that SA improved the alpha diversity and the relative abundance of thermophilic bacteria, mainly Actinobacteria, increased by 12.955% compared with CK. A redundancy analysis (RDA) showed that Actinobacteria was positively correlated with the transformation of carbon.

Hydrogen peroxide plus ascorbic acid enhanced organic matter deconstructions and composting performances via changing microbial communities.

This work aims to investigate the influence of hydrogen peroxide (H2O2) and ascorbic acid (ASCA) on the physicochemical characteristics, organic matter (OM) deconstructions, humification degree and succession of bacterial communities for co-composting of bagasse pith and dairy manure. The results indicated that H2O2 and ASCA accelerated the degradation of lignocellulose, improved the transformation of dissolved organic matter (DOM), and enhanced the content of humic substance (HS) and the degree of its aromatization. The bacterial communities were significantly changed in the presence of additives, in which the relative abundances of Firmicutes and Actinobacteria significantly increased. Redundancy analysis (RDA) indicated that the degradation of OM and lignocellulose more influenced the bacterial community compositions. Conclusively, adding H2O2 and ASCA accelerated lignocellulose degradation efficiency, and improved the composting process, which provided an optimized method to dispose of lignocellulose wastes and livestock manure.

Pyrene derivative-functionalized mesoporous silica-Cu2+ hybrid ensemble for fluorescence "turn-on" detection of H2S and logic gate application in aqueous media.

The ensemble system PyH-SBA-15-Cu2+ was obtained via coordination interaction of pyrene derivative-functionalized mesoporous SBA-15 and Cu2+, and applied for the selective and sensitive detection of H2S over pH 6.0-12.0 in aqueous media. The sensing strategy was designed on the basis of the H2S-induced dissolution of Cu2+ from PyH-SBA-15-Cu2+. Cu2+ has good binding affinity to N atoms in PyH-SBA-15; therefore, the organic-inorganic hybrid ensemble PyH-SBA-15-Cu2+ was formed, which is nonfluorescent in aqueous solution because of the Cu2+-promoted emission quenching of PyH-SBA-15. The addition of H2S induces the dissolution of PyH-SBA-15-Cu2+ by the formation of stable CuS, thereby producing fluorescence revival of PyH-SBA-15. The correlative "turn-on" fluorescence signals of this ensemble system are linearly proportional to [H2S] in the concentration region of 0-1.0 × 10-4 M, showing a low detection limit of 3.7 × 10-7 M. Other common anions do not induce distinct fluorescence changes. When using the fluorescence intensity signal changes of PyH-SBA-15 as outputs and Cu2+ and S2- as inputs, PyH-SBA-15 can act as an XNOR logic gate.

A compost-derived thermophilic microbial consortium enhances the humification process and alters the microbial diversity during composting.

This work was conducted to assess the influence of a compost-born multifunctional thermophilic microbial consortium (CTMC) on the physico-chemical parameters, organic matter (OM) transformation and dynamic succession of microbial communities in dairy manure-sugarcane leaves co-composting. The results revealed that CTMC inoculation not only improved the bio-degradation of OM and lignocellulose but also distinctly enhanced the aromaticity and stability degrees of dissolved organic matter and humic substance (HS). Additionally, the complexity and diversity of bacterial and fungal community increased after inoculation. Redundancy analysis indicated that the microbial communities compositions and the physico-chemical parameters interacted with each other in humification process. The dominated bacterial and fungal species related to lignocellulose degradation and humification process were also detected. Accordingly, this research could put forward a possible optimized inoculation strategy to enhance the mineralization of organic carbon, accelerate the lignocellulose degradation and promote the humification process in solid organic waste composting.

Exogenous additive ferric sulfate regulates sulfur-oxidizing bacteria in cow manure composting to promote carbon fixation.

Enhancing carbon fixation in the composting process was of great significance in the era of massive generation of organic solid waste. In this study, the experimental results showed that the contents of dissolved organic matter (DOM) in the experimental group (CT) were 37.58% higher than those in the control group (CK). The CO2 emission peaked on day 5, and the value of CK was 1.34 times that of CT. Significant differences were observed between the contents of sulfur fractions in CT and CK. This phenomenon may be due to the suppression of sulfur-reducing gene expression in CT. On day 51 of composting, the abundance of sulfur-oxidizing bacteria (SOB) Rhodobacter (5.33%), Rhodovulum (14.76%), and Thioclava (23.83%) in CT was higher than that in CK. In summary, the composting fermentation regulated by Fe2(SO4)3 increased the sulfate content, enhanced the expression of sulfur-oxidizing genes and SOB, and ultimately promoted carbon sequestration during composting.

Modifying humus-phosphorus-arsenic interactions in sludge composting: The strengthening of phosphorus availability and arsenic efflux detoxification mechanisms.

Arsenic (As) in sewage sludge poses a significant threat to environmental and human health, which has attracted widespread attention. This study investigated the value of adding sodium percarbonate (SP) on phosphorus (P) availability and As efflux detoxification through HS-P-As interactions. Due to the unique structure of humus (HS) and the similar chemical properties of P and As, the conditions for HS-P-As interaction are provided. This study discussed the content, morphology and microbial communities of HS, P and As by using metagenomic and correlation analysis. The results showed that the humification index in the experiment group (SPC) was 2.34 times higher than that in the control group (CK). The available phosphorus (AP) content of SPC increased from 71.09 mg/kg to 126.14 mg/kg, and SPC was 1.11 times that of CK. The relative abundance of ACR3/ArsB increased. Pst, Actinomyces and Bacillus commonly participated in P and As conversion. The correlation analysis revealed that the humification process was enhanced, the AP was strengthened, and the As was efflux detoxified after SP amendment. All in all, this study elucidated the key mechanism of HS-P-As interaction and put forward a new strategy for sewage sludge resource utilization and detoxification.

Heavy metal tolerance and detoxification mechanism mediated by heavy metal resistance genes in compost habitat.

Heavy metal pollution from compost is one of the most concerned environmental problems, which poses a threat to the ecosystem and human health. This study aims to reveal the heavy metal tolerance and detoxification mechanism mediated by heavy metal resistance genes (HMRGs) in compost habitat through metagenomics combined with chemical speciation analysis of heavy metals. The results showed that there were 37 HMRGs corresponding to 7 common heavy metal(loid)s in composting, and they had the ability to transform heavy metals into stable or low-toxic speciation by regulating enzyme transport, redox, methylation, etc. This study summarized the heavy metal metabolism pathway mediated by HMRGs, providing a new perspective for understanding the transformation of heavy metals in the composting process.

Incorporating microbial inoculants to reduce nitrogen loss during sludge composting by suppressing denitrification and promoting ammonia assimilation.

To address the challenge of increasing nitrogen retention in compost, this study investigated the effects of microbial communities on denitrification and ammonia assimilation during sludge composting by inoculating microbial inoculants. The results showed that the retention rates of total Kjeldahl nitrogen (TKN) and humic acid (HA) in MIs group (with microbial inoculants) were 4.94 % and 18.52 % higher than those in the control group (CK), respectively. Metagenomic analysis showed that Actinobacteria and Proteobacteria were identified as main microorganisms contributing to denitrification and ammonia assimilation. The addition of microbial agents altered the structure of the microbial community, which in turn stimulated the expression of functional genes. During cooling period, the ammonia assimilation genes glnA, gltB and gltD in MIs were 15.98 %, 24.84 % and 32.88 % higher than those in CK, respectively. Canonical correspondence analysis revealed a positive correlation between the dominant bacterial genera from the cooling stage to the maturity stage and the levels of NO3--N, NH4+-N, HA, and TKN contents. NH4+-N was positively correlated with HA, indicating NH4+-N might be incorporated into HA. Heat map and network analyses revealed NH4+-N as a key factor affecting functional genes of denitrification and ammonia assimilation, with Nitrospira identified as the core bacteria in the microbial network. Therefore, the addition of microbial agents could increase nitrogen retention and improve compost product quality.

Competition and interaction between DNRA and denitrification in composting ecosystems: Insights from metagenomic analysis.

This study investigated denitrification and dissimilatory nitrate reduction to ammonium (DNRA) competition for nitrite in composting of sugarcane pith and cow manure. Metagenomic analysis showed that Actinobacteria was the main DNRA microorganism. During heating phase and thermophilic phase, the abundances of denitrification functional genes (nirK and nirS decreased by 40.22% and 98.60%, respectively) and DNRA functional genes (nirB, nirD increased by 195.24% and 176.61%, and nrfA decreased by 45%, respectively) showed different trends. Interestingly, the abundance of nrfA increased by 250% during cooling and maturity phases. Mantel test revealed that competition between denitrification and DNRA microorganisms for NO2--N limited the succession of their respective communities (P < 0.01). Network analysis showed that unclassified Solirubrobacterales, Altererythrobacter and Microbacterium were the key microorganisms in DNRA microbial communities. The results provided new insights into the key microorganisms and their driving factors affecting DNRA and nitrogen management in the composting ecosystems.

A potential heavy metals detoxification system in composting: Biotic and abiotic synergy mediated by shell powder.

Regulating heavy metal resistance genes (HMRGs) was an effective method for heavy metal resistant bacteria (HMRB) to cope with heavy metal stress during dairy manure composting. This research aimed to investigate heavy metal detoxification mediated by shell powder (SP) in composting and the response of HMRB and HMRGs to changes in heavy metal bioavailability during composting. Research showed that SP additive reduced the bioavailability of Zu, Cu, and Mn by 10.64%, 13.90% and 14.14%, respectively. SP increased the composition percentage of humic acid (HA) in humus (HS) by 8%. SP enhanced the resistance of Actinobacteria to heavy metals and improved the regulation of HMRGs. Correlation analysis demonstrated that the bioavailability of heavy metals was positively correlated with most HMRGs. HA was significantly negatively correlated with the bioavailability of Zn, Cu and Mn. Therefore, SP additive could be a novel strategy for heavy metals detoxification during composting.

Antibiotic resistance gene profiles and evolutions in composting regulated by reactive oxygen species generated via nano ZVI loaded on biochar.

In this study, nano zero-valent iron loaded on biochar (BC-nZVI) was analyzed for its effects on antibiotic resistance genes (ARGs) in composting. The results showed that BC-nZVI increased reactive oxygen species (ROS) production, and the peak values of H2O2 and OH were 22.95 % and 55.30 % higher than those of the control group, respectively. After 65 days, the relative abundances of representative ARGs decreased by 56.12 % in the nZVI group (with BC-nZVI added). An analysis of bacterial communities and networks revealed that Actinobacteria, Proteobacteria, and Firmicutes were the main hosts for ARGs, and BC-nZVI weakened the link between ARGs and host bacteria. Distance-based redundancy analysis showed that BC-nZVI altered the microbial community structure through environmental factors and that most ARGs were negatively correlated with ROS, suggesting that ROS significantly affected the relative abundance of ARGs. According to these results, BC-nZVI showed potential for decreasing the relative abundance of ARGs in composting.

Metagenomic insights into role of red mud in regulating fate of compost antibiotic resistance genes mediated by both direct and indirect ways.

In this study, the amendment of red mud (RM) in dairy manure composting on the fate of antibiotic resistance genes (ARGs) by both direct (bacteria community, mobile genetic elements and quorum sensing) and indirect ways (environmental factors and antibiotics) was analyzed. The results showed that RM reduced the total relative abundances of 10 ARGs and 4 mobile genetic elements (MGEs). And the relative abundances of total ARGs and MGEs decreased by 53.48% and 22.30% in T (with RM added) on day 47 compared with day 0. Meanwhile, the modification of RM significantly increased the abundance of lsrK, pvdQ and ahlD in quorum quenching (QQ) and decreased the abundance of luxS in quorum sensing (QS) (P < 0.05), thereby attenuating the intercellular genes frequency of communication. The microbial community and network analysis showed that 25 potential hosts of ARGs were mainly related to Firmicutes, Proteobacteria and Actinobacteria. Redundancy analysis (RDA) and structural equation model (SEM) further indicated that RM altered microbial community structure by regulating antibiotic content and environmental factors (temperature, pH, moisture content and organic matter content), which then affected horizontal gene transfer (HGT) in ARGs mediated by QS and MGEs. These results provide new insights into the dissemination mechanism and removal of ARGs in composting process.

Iron reduction in composting environment synergized with quinone redox cycling drives humification and free radical production from humic substances.

The aim of this paper was to investigate the influence of Fe (III) on humification and free radicals evolution. The experimental data showed that the experimental group (CT) with Fe2(SO4)3 had a better degree of humification than the control group (CK). The humic substances (HS) content was 10% higher in CT (23.94 mg·g-1) than in CK (21.54 mg·g-1) in the final. Fe (III) contributed significantly to the formation of free radicals in HS. The amount of H2O2 in CT increased to 74.8 mmol·kg-1, while CK was only 46.5 mmol·kg-1. The content of semiquinone free radical was 10.32 × 1011 spins/mm3 in CT, 5.11 × 1011 spins/mm3 in CK in the end. Several iron-reducing bacteria were detected in composting, among which Paenibacillus was dominant. The above findings suggested that the application of Fe2(SO4)3 enhanced the iron reduction synergistic quinone redox cycling and promoted the generation of free radicals during the humification of composting.

A novel carbon-nitrogen coupled metabolic pathway promotes the recyclability of nitrogen in composting habitats.

This study revealed a novel carbon-nitrogen coupled metabolic pathway. Results showed that the addition of inorganic carbon sources slowed down the decomposition of urea and conserved more nutrients in composting. Metagenomic analysis showed that the main bacteria involved in this new pathway were Actinobacteria, Proteobacteria and Firmicutes. During the late composting period, the dominant genus Microbacteium involved in denitrification accounted for 22.18% in control (CP) and only 0.12% in treatment group (T). Moreover, ureC, rocF, argF, argI, argG were key genes involved in urea cycle. The abundance of functional gene ureC and denitrification genes decreased in thermophilic and cooling phases, respectively. The genes hao, nosZ, ureA and nifH were more closely associated with Chloroflexi_bacterium and Bacillus_paralichenformis. In conclusion, composting habitats with additional inorganic carbon sources could not only weaken denitrification but also allow more nitrogen to be conserved through slow-release urea to improve resource utilization and decrease the environmental risk.