Effects of antibiotics stress on root development, seedling growth, antioxidant status and abscisic acid level in wheat (Triticum aestivum L.).

The veterinary antibiotics contamination in agroecosystems is a substantial problem globally. However, little is known about their toxicity to crops, especially in wheat. This study evaluated the phytotoxic effects of the two most representative antibiotics, namely oxytetracycline (OTC) and enrofloxacin (ENR), on seed germination, seedling growth, root elongation and antioxidant status in wheat, and investigated the response of abscisic acid (ABA) to antibiotic stress and its underlying mechanism. The results showed that OTC and ENR under the experimental concentrations (5, 10, 20, 40 and 80 mg·L-1) had no influence on seed germination of wheat. The reduced root length, fresh weight and surface area were observed when the concentrations of OTC and ENR were higher than 10 mg·L-1 and 5 mg·L-1, respectively. High concentrations (＞40 mg·L-1) of antibiotics dramatically decreased the root length, fresh weight, root numbers and surface area as well as the number of stele cells and stele area. The activity of catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD), and malondialdehyde (MDA) content in shoots and roots were increased with the increasing OTC and ENR concentrations. High concentrations (＞40 mg·L-1) of antibiotics improved ABA content and enhanced the transcription levels of genes related to ABA biosynthesis (TaNCED1 and TaNCED2) and metabolism (TaABA8'OH1-A and TaABA8'OH2-A) in shoots and roots of wheat seedlings. Wheat seedlings had relatively strong sensitivity to low concentration (5 mg·L-1) of ENR. These results suggest that OTC and ENR modulate root development and seedling growth by regulating ABA level and antioxidant defense system in wheat.

[Effects of Long-term Fertilizations on the Organic Carbon Components of Soil Macroaggregates and the Yield of Wheat in Wheat Fields on the Loess Plateau].

Soil macro-aggregates are the main location for soil organic carbon （SOC） sequestration, which is of great significance to improve soil fertility. This study aimed to understand the mechanisms of the organic carbon （OC） sequestration in macroaggregates and improve crop yield in wheat fields on the loess plateau. With the aggregate-density fractionation method, an eight-year experiment was conducted to investigate the following three factors： ① the effects of long-term fertilization on OC fractions within macroaggregates； ② the variation characteristics of OC fractions within macroaggregates, including coarse particulate organic carbon （cPOC）, fine particulate organic carbon （fPOC）, intra-microaggregate particulate organic carbon （iPOC）, free silt and clay particulate carbon （s+c_f）, and intra-microaggregate silt and clay particulate carbon （s+c_m）； ③ and the relationships between them and SOC input and yield formation. The treatments included no fertilization （CK）, farmer pattern （NP）, optimized fertilizers pattern （NPK）, optimized fertilizers + organic fertilizers pattern （NPKM）, and optimized fertilizers + biological organic fertilizers pattern （NPKB）. The results showed that the application of organic and chemical fertilizer （NPKM and NPKB） improved significantly the SOC content in macroaggregates compared with that in the single fertilizer treatment （NP and NPK）, which had a greater increase in SOC content in macroaggregates than that of the soil. All fertilization treatments had a tendency to increase the content of fractions iPOC, fPOC, and iPOC in macroaggregates, but silt and clay carbon （s+c_f and s+c_m） contents were decreased. The application of manure combined with chemicals markedly increased the allocations of fractions cPOC, fPOC, and iPOC reserves, but it greatly decreased （s+c_f） reserves allocation. However, the application of chemical fertilizers only significantly increased the proportion of cPOC reserves in macroaggregates. Correlation analysis showed that there were significant positive correlations among wheat grain yield and OC fractions （cPOC and fPOC） contents, SOC content, the OC content of >0.25 mm macroaggregates, and SOC input, and the correlation coefficient was 0.645-0.883. In conclusion, long-term fertilization, especially combined with organic fertilizer, could promote the free silt and clay carbon fraction （s+c_f） to transfer into other forms of OC components through the increase in soil carbon input in the wheat field of the loess plateau. Furthermore, the OC content of macroaggregates was increased overall, providing a good soil environment for crop yield.

[Response Characteristics of Soil Fungal Community Structure to Long-term Continuous Cropping of Pepper].

This study aimed to clarify the effect of long-term continuous cropping of pepper on soil fungal community structure, reveal the mechanism of continuous cropping obstacles, and provide a theoretical basis for the ecological safety and sustainable development of pepper industry. We took the pepper continuous cropping soil in the vegetable greenhouse planting base of Tongren City as the research object. The diversity and community structure of fungi in farmland soil were analyzed using Illumina MiSeq high-throughput sequencing, the responses of soil physio-chemical properties and fungal community characteristics to long-term continuous pepper cropping were discussed, and the relationships between the characteristics of fungal community structure and environmental factors were determined using CCA and correlation network analysis. The results showed that with the extension of pepper continuous cropping years, the soil pH value and organic matter (OM) content decreased, total phosphorus (TP) and available phosphorus (AP) contents increased, hydrolyzed nitrogen (AN) and available potassium (AK) contents decreased first and then increased, and total nitrogen (TN) and total potassium (TK) contents did not change significantly. Long-term continuous cropping decreased the Chao1 index and observed species index and decreased the Shannon index and Simpson index. The change in continuous cropping years had a significant effect on the relative abundance of soil fungal dominant flora. At the phylum level, the relative abundance of Mortierellomycota decreased with the extension of pepper continuous cropping years, the relative abundance of Ascomycota increased first and then decreased, and the relative abundance of Basidiomycota decreased first and then increased. At the genus level, with the increasing of pepper continuous cropping years, the relative abundance of Fusarium increased, and the relative abundance of Mortierella and Penicillium decreased. In addition, long-term continuous cropping simplified the soil fungal symbiosis network. CCA analysis indicated that pH, OM, TN, AN, AP, and AK were the driving factors of soil fungal community structure, and correlation network analysis showed that pH, OM, TN, TP, TK, AN, AP, and AK were the driving factors of soil fungal community structure, including Fusarium, Lophotrichus, Penicillium, Mortierella, Botryotrichum, Staphylotrichum, Plectosphaerella, and Acremonium. In conclusion, continuous cropping changed the soil physical and chemical properties, affected the diversity and community structure of the soil fungal community, changed the interaction between microorganisms, and destroyed the microecological balance of the soil, which might explain obstacles associated with continuous cropped pepper.

[Changes in Physical Fractions within Soil Aggregates Under Nitrogen Reduction and Film Mulching Measures in Dryland Wheat Field].

We studied the changes in various physical fractions within aggregates in the arid plateau of southern Shanxi Province, which has great significance for synergistically improving soil fertility and crop productivity in this region. Bulk soil samples were collected from 0-20 cm layers during a 7-year long-term experiment in Hongtong County, Shanxi Province. Wheat grain yields, SOC concentrations, proportions, and OC contents within soil aggregates were analyzed. OC contents included： unprotected coarse particulate organic carbon within macroaggregate （M-cPOC） and fine particulate organic carbon within macroaggregate （M-fPOC）, physically protected intra-aggregate particulate organic carbon within macroaggregate （M-iPOC）, chemically/biochemically protected mineral organic carbon within macroaggregate （M-MOC）, unprotected fine particulate organic carbon within microaggregate （m-fPOC）, physically protected intra-aggregate particulate organic carbon within microaggregate （m-iPOC）, and chemically/biochemically protected mineral organic carbon within microaggregate （m-MOC）. The treatments were ① farmer fertilization （FP）, ② nitrogen reduction monitoring and control fertilization （MF）, ③ nitrogen reduction monitoring and control fertilization plus ridge film and furrow sowing （RF）, and ④ nitrogen reduction monitoring and control fertilization plus flat film hole sowing （RF）. The results showed that compared with that in the FP treatment, MF reduced SOC concentration while maintaining wheat grain yield, RF and FH synergistically improved soil fertility and crop yield, especially for the FH with SOC concentration, and wheat grain yield increased by 8.44% and 48.86%, respectively. MF significantly reduced the content of M-cPOC, RF significantly increased the content of M-iPOC, and FH significantly increased the contents of M-fPOC, M-iPOC, M-MOC, and m-iPOC by 64.00%, 98.39%, 6.16%, and 17.48%, respectively. In addition, combined with redundancy analysis, we found that the M-iPOC fraction played a major role in increasing SOC concentration and wheat grain yield, with a contribution rate of 61.5%. Therefore, the contribution of macroaggregates to soil fertility and crop productivity was higher than that of microaggregates in the arid plateau area of southern Shanxi, and flat film hole sowing could increase the content of M-iPOC, thereby synergistically increasing SOC sequestration and wheat grain yield, which could promote this cultivation technology in the region and even in the country's arid agricultural areas.

Effects of different fertilization treatments on soil microbial functional diversity of dry tableland wheat field in south Shanxi Province.

Understanding the effects of different fertilization treatments on microbial functional diversity in loess tableland wheat soil in south Shanxi Province can provide the theoretical basis from the perspective of microbial functional diversity for chemical fertilizer reduction, wheat yield increase, and soil fertility improvement in dryland soil. We conducted a long-term field experiment with seven fertilization treatments in winter wheat cultivation area of loess tableland in south Shanxi Province, including straw charcoal fertilizer (SF), bacterial fertilizer (BF), organic fertilizer (OF), humic acid fertilizer (HF), monitoring fertilizer (MF), farmer fertilizer (FF) and no fertilizer (CK). We employed Biolog-ECO microplate technique to investigate the differences of carbon source utilization capacity and functional diversity of soil microorganisms. The results showed that all the fertilization treatments could improve the metabolic activity and functional diversity of soil microbial community. Carbon source utilization was the most efficient in SF, with the overall soil microbial utilization ability of the 31 carbon sources and the utilization ability of different guilds of carbon sources being improved. Functional diversity, richness, and dominance based on microbial carbon sources utilization were significantly higher in SF treatment than that under other five treatments, and the evenness was higher than BF. Results of principal component analysis (PCA) and biclustering heatmap analysis showed that different fertilization treatments had significant effects on the metabolic function of microbial community. SF treatment could promote the functional diversity of soil microbial community, especially for the utilization of carbohydrates, carboxylic acids and amino acids. In conclusion, straw charcoal fertilizer had positive effects on soil microbial activity in wheat soil of loess tableland in south Shanxi Province.

Rejuvenation of iron oxides enhances carbon sequestration by the 'iron gate' and 'enzyme latch' mechanisms in a rice-wheat cropping system.

The 'enzyme latch' theory believes that oxygen constraints on phenol oxidase can restrain the activity of hydrolytic enzymes responsible for decomposition, while the 'iron (Fe) gate' theory suggests that Fe oxidation can decrease phenol oxidase activity and enhance Fe-lignin complexation under oxygen exposure. The objective of this study was to explore the roles of the 'enzyme latch' and 'Fe gate' mechanisms in regulating soil organic carbon (SOC) sequestration in a rice-wheat cropping system subjected to six fertilization treatments: control (CT), chemical fertilizer (CF), CF plus manure (CFM), CF plus straw (CFS), CF plus manure and straw (CFMS), and CF plus organic-inorganic compound fertilizer (OICF). Soil samples were collected after the rice and wheat harvests and wet sieved into large macroaggregates, small macroaggregates, microaggregates, and silt and clay particles. Variations in amorphous and free Fe oxides, Fe-bound organic carbon and phenol oxidase activity were examined. After nine years, compared with the initial soil, the activation degree of free Fe oxides increased by 1.3- to 1.6-fold and the topsoil SOC stock increased by 13-61% across all treatments. Amorphous Fe oxide content, phenol oxidase activity and aggregate mean-weight diameter were higher after the wheat harvest than after the rice harvest. Amorphous Fe oxide content was positively correlated with Fe-bound organic carbon content (P < 0.001) but negatively correlated with phenol oxidase activity (P < 0.001). Therefore, seasonal alternation of wetting and drying can progressively drive the rejuvenation of Fe oxides and simultaneously affect the activity of phenol oxidase. Oxidative precipitation of amorphous Fe oxides promoted the formation of organo-Fe complexes and macroaggregates, while flooding of the paddies decreased the activity of phenol oxidase, thereby resulting in year-round hindered decomposition. Organic fertilization strengthened the roles of the 'Fe gate' and 'enzyme latch' mechanisms, and thus accelerated SOC sequestration in the rice-wheat cropping system.

Effects of Seven-Year Fertilization Reclamation on Bacterial Community in a Coal Mining Subsidence Area in Shanxi, China.

The restoration of soil fertility and microbial communities is the key to the soil reclamation and ecological reconstruction in coal mine subsidence areas. However, the response of soil bacterial communities to reclamation is still not well understood. Here, we studied the bacterial communities in fertilizer-reclaimed soil (CK, without fertilizer; CF, chemical fertilizer; M, manure) in the Lu'an reclamation mining region and compared them with those in adjacent subsidence soil (SU) and farmland soil (FA). We found that the compositions of dominant phyla in the reclaimed soil differed greatly from those in the subsidence soil and farmland soil (p < 0.05). The related sequences of Acidobacteria, Chloroflexi, and Nitrospirae were mainly from the subsided soil, whereas those of Alphaproteobacteria, Planctomycetes, and Deltaproteobacteria were mainly derived from the farmland soil. Fertilization affected the bacterial community composition in the reclaimed soil, and bacteria richness and diversity increased significantly with the accumulation of soil nutrients after 7 years of reclamation (p < 0.05). Moreover, soil properties, especially SOM and pH, were found to play a key role in the restoration of the bacterial community in the reclaimed soil. The results are helpful to the study of soil fertility improvement and ecological restoration in mining areas.

Coupling between nitrogen-fixing and iron(III)-reducing bacteria as revealed by the metabolically active bacterial community in flooded paddy soils amended with glucose.

Biological nitrogen fixation can contribute to maintaining the nitrogen balance and reducing the risk of environmental pollution caused by nitrogen fertilizer application in flooded paddy soils. Microorganisms associated with microbial iron [Fe(III)] reduction are prevalent and presumed to be closely linked with biological nitrogen fixation in flooded paddy soils. The relationship between the nitrogen-fixing bacteria (NFB) and Fe(III)-reducing bacteria (FeRB) and their responses to organic carbon addition were investigated based on the metabolically active bacterial community in flooded paddy soils amended with/without glucose (CK: 0 mol C kg-1 soil; OC: 0.1 mol C kg-1 soil). Both putative NFBs and FeRBs were affiliated to the phyla Firmicutes and Proteobacteria, which were the two most abundant phyla in the metabolically active bacterial community. Glucose addition remarkably altered the community structures of the putative NFBs and FeRBs during a 40-day incubation, and the relative abundances of putative NFBs and FeRBs in the OC treatment increased by 0.21%-1.62% and 2.22%-14.82% relative to the CK treatment, respectively, during the later stage of incubation. The putative FeRBs co-occurred with NFBs and hydrogen-oxidizing bacteria, and the relative abundances of NFBs and hydrogen-oxidizing bacteria showed significant positive correlation with that of respiratory FeRBs. Some FeRBs could also be capable of nitrogen fixation and/or hydrogen oxidation. Thus, it might be feasible to enhance biological nitrogen fixation efficiency by promoting the metabolic activities of FeRBs (such as by adding glucose), which contribute directly to biological nitrogen fixation associated with nitrogen-fixing Fe(III) reducers and indirectly by reducing the suppression of hydrogen on nitrogen fixation associated with hydrogen-dependent Fe(III) reducers.

Highly reactive nanomineral assembly in soil colloids: Implications for paddy soil carbon storage.

Mineral availability for carbon (C) binding is a key regulator of soil C storage, yet little is known about the highly reactive nanomineral assembly in the paddy soil colloids. Here, using high-resolution transmission electron microscopy (HRTEM), solid-state 27Al and 29Si nuclear magnetic resonance (NMR) spectroscopy and X-ray photoelectron spectroscopy (XPS), we investigated the coordination nature of short-range-ordered (SRO) minerals in water-dispersible colloids that were isolated from the paddy soil under different six-year fertilization regimes. Our results showed that organic fertilization not only promoted the transformation of crystalline minerals to SRO phases in the bulk soils but also increased the concentrations of Fe, Al and Si in the soil colloids compared to chemical fertilization alone, and thus enhanced the accumulation of organic C in both the bulk soils and the soil colloids. The HRTEM images revealed that water-dispersible colloids in all soils, regardless of treatment, were composed of crystalline Fe nanominerals (with some Al/Si) and SRO-Al/Si nanominerals (with some Fe) associated with organic C. Furthermore, the combined results from the 27Al and 29Si NMR spectroscopy and XPS not only confirmed the presence of SRO-Al/Si nanoparticles as Si-rich allophane and phytolith but also demonstrated that organic fertilization promoted the transformation of aluminosilicates to SRO-Al/Si nanominerals in soil colloids. Together, these findings indicate that six-year organic fertilization promotes the formation of SRO minerals (e.g., ferrihydrite, Si-rich allophane and Fe-substituted allophane, as well as phytolith) in soils and modulates the assembly of organo-mineral complexes possibly by driving the biogeochemical cycles of Fe, Al, Si and specific organic ligands, thus contributing to the long-term storage of C in paddy soils.

Year-round film mulching system with monitored fertilization management improve grain yield and water and nitrogen use efficiencies of winter wheat in the dryland of the Loess Plateau, China.

Year-round film mulching in winter wheat field facilitates rainwater storage in summer fallow period and reduces water evaporation in growing reason, and then increases water use efficiency in the dryland of the Loess Plateau, China. Optimized fertilization further promotes fertilizer utilization efficiencies. In this study, plastic film mulching was extended from plant growth season to summer fallow, and fertilizers were applied by monitoring soil nutrient availability. Field trials were conducted in the dryland of the Loess Plateau over 4 years by using four types of cultivation to investigate the effects of year-round plastic film mulching with monitored fertilization on utilization efficiencies of rainwater and nitrogen (N), and winter wheat yield. The four types of cultivation were farmer practice (FP), ridge-furrow with plastic film mulching system plus conventional fertilization(RPCF), ridge-furrow with plastic film mulching system plus monitored fertilization (RPFM), and flat soil surface with plastic film mulching system plus monitored fertilization (FPFM). Our results indicate that the average yield of winter wheat in RPFM and FPFM treatments was 4491 kg ha-1. Compared with FP treatment, the combined effects of monitored fertilization and film mulching(RPFM and FPFM treatments) could increase grain yield in the range of 24.7 to 42.1%. The film mulching extended to the fallow season increased the water storage in 2 m depth of soil profile, and the amount of soil water storage in the summer fallow period increased by 27 to 30% in FPFM treatment than FP treatment. After 4-year consecutive planting of wheat, the accumulation of nitrate-N in 2 m soil reached 277 kg·ha-1 in the FP treatment, which is 87.7% higher than of the level at the beginning of the experiment. Seventy-five percent of nitrate-N was distributed in the soil layer of 0-120 cm. In addition, the residual nitrate-N showed downward leaching with rainfall during the experiment. The RPFM and FPFM treatments reduced the apparent loss and residual levels of soil N, whereas increased its apparent mineralization compared with FP treatment. The FPFM treatment exhibited a greater utilization of residual nitrate-N from previous years and showed a higher amount of the mineralized N from soil organic matter, therefore leading to a relatively high apparent utilization rate of N (56.7%). Considering both grain yield production and utilization efficiencies of water and N, FPFM with year-round mulching was the most effective cultivation measure for winter wheat in the Loess Plateau.

[Change characteristics of soil moisture and nutrients in rain-fed winter wheat field under different fertilization modes in Southern Shanxi of China during summer fallow period].

In 2009-2011, a field experiment was conducted in a rain-fed winter wheat field in Southern Shanxi of China to study the effects of different fertilization modes on the change characteristics of soil moisture and nitrate-N contents in 0-200 cm layer and of soil available phosphorus (Oslen-P) and potassium contents in 0-40 cm layer during summer fallow period (from June to September). Three fertilization modes were installed, i. e., conventional fertilization (CF), recommended fertilization (RF), and ridge film furrow planting (RFFP) combined with straw mulch. The results showed that the rainfall in summer fallow period could complement the consumed water in 0-200 cm soil layer in dryland wheat field throughout the growth season, and more than 94% of the water storage was in 0-140 cm soil layer, with the fallow efficiency ranged from 6% to 27%. The rainfall in summer fallow period caused the soil nitrate-N moving downward. 357-400 mm rainfall could make the soil nitrate-N leaching down to 100 cm soil layer, with the peak in 20-40 cm soil layer. Straw mulching or plastic film with straw mulch in summer fallow period could effectively increase the Oslen-P and available K contents in 0-40 cm soil layer, and the accumulative increment in three summer fallow periods was 16-45% and 36-49%, respectively. Among the three modes, the binary coverage mode of RFFP plus furrow straw mulching had the best effect in maintaining soil water and fertility. The accumulative water storage and mineral N in 0-200 cm soil layer in three summer fallow periods were up to 215 mm and 90 kg x hm(-2), and the accumulative Oslen-P and available K contents in plough layer were increased by 2.7 mg x kg(-1) and 83 mg x kg(-1), respectively, being significantly higher than those in treatments CF and RF. There were no significant differences in the change characteristics in the soil moisture and nutrients between treatments CF and RF.

[Effects of nitrogen application and ridge film furrow planting on water use of winter wheat in dry land of south Shanxi].

A 2-year (2008-2010) field experiment was conducted to study the effects of basal dressing nitrogen, topdressing nitrogen, and ridge film furrow planting on the 0-2 m soil moisture status and the grain yield and water use efficiency of winter wheat in rain-fed area of South Shanxi Province. In all treatments, the soil moisture status during winter wheat growth period had the same change trend, being increased steadily from pre-sowing to revival stage and decreased sharply from revival stage to heading stage, and then increased gradually till maturity stage. From revival stage to heading stage, the soil water consumption was the most. Increasing nitrogen basal application rate or topdressing nitrogen increased the soil water consumption, widened the soil moisture active layer, and deepened the relatively stable layer. Topdressing nitrogen increased grain yield significantly; ridge film furrow planting decreased soil water consumption obviously. The water use efficiency under ridge film furrow planting was 23.4% and 39.1% higher than that under conventional planting system in 2009 and 2010 (P < 0.01). The grain yield under ridge film furrow planting plus top-dressing nitrogen was 3643 kg x hm(-2), which was significantly higher than that under single ridge film furrow planting or topdressing nitrogen, displaying a preferable water-fertilizer coupling effect.