Effects of Foxp3 gene silencing on the expression of inflammatory cytokines and the proliferation and migration of human periodontal ligament fibroblasts in an inflammatory environment / 华西口腔医学杂志

OBJECTIVES@#This study aimed to clarify the effects of Foxp3 silencing on the expression of inflammatory cytokines in human periodontal ligament cells (hPDLFs) in an inflammatory environment and on cell proliferation and invasiveness, as well as to explore the role of Foxp3 gene in the development of periodontitis.@*METHODS@#An small interfering RNA (siRNA) construct specific for Foxp3 was transfected into hPDLFs. Foxp3 silencing efficiency was verified by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, and the siRNA with the optimum silencing effect of Foxp3 gene was screened. Using lipopolysaccharide to simulate an inflammatory environment in vitro, CCK-8 detected the effect of silencing Foxp3 on hPDLFs proliferation under inflammatory conditions. Wound-healing experiments and transwell assays were conducted to detect the effect of silencing Foxp3 on hPDLF migration under inflammatory conditions. The expression of the inflammatory cytokines interleukin (IL)-6 and IL-8 was detected by RT-PCR and Western blotting under inflammatory conditions.@*RESULTS@#After siRNA transfection, RT-PCR and Western blotting analyses showed that the expression of Foxp3 mRNA in the Foxp3-si3 group decreased significantly (t=21.03, P<0.000 1), and the protein expression of Foxp3 also decreased significantly (t=12.8, P<0.001). In the inflammatory environment, Foxp3 gene silencing had no significant effect on hPDLFs proliferation (P>0.05), and Foxp3 gene silencing promoted hPDLFs migration (P<0.05). Moreover, the expression of IL-6 and IL-8 increased (P<0.05).@*CONCLUSIONS@#In an inflammatory environment, Foxp3 gene silencing promoted hPDLFs migration but had no significant effect on hPDLFs proliferation. The expression of inflammatory factors expressed in hPDLFs increased after Foxp3 gene silencing, indicating that Foxp3 gene inhibited inflammation in periodontitis.

The correlation between chronic periodontitis and IL-6 and IL-17 expression in serum and saliva of Kazak and Han subjects / 实用口腔医学杂志

IL-6 and IL-17 levels in saliva and serum were measured by ELISA in 116 cases of chronic periodontitis(CP)in Mulei district of Xinjiang,including 62 cases of Han and 54 of Kazak.Health controls included 50 subjects of Han and 45 of Kazak.IL-6 and IL-17 levels in serum and saliva were higher in CP groups than in the controls(P <0.05),and correlated with the severity of CP(P <0.05),in Kazak CP group were higher than in Han CP group(P <0.05).

Effect of Initial Periodontal Therapy on Eradiation Rate of Gastric Helicobacter Pylori in Patients with Gastric Ulcer / 中国药师

Objective:To investigate the adjuvant effect of initial periodontal therapy by observing the eradiation rate of gastric helicobacter pylori( Hp) in the patients with gastric ulcer treated by the therapy. Methods:Totally 88 patients with stomach ulcer and chronic periodontitis were enrolled and randomly divided into two groups. The control group was treated by triple therapy,the experimental group was treated by initial periodontal therapy additionally with the treatment course of one month. The changes of Hp in stomach and periodontium were observed and the eradiation rate of Hp was compared between the two groups respectively after 1-, 3-, 6-and 12-month treatment. Results:After 3, 6 and 12 months, the eradiation rate in the experimental group was significantly higher than that in the other group (P < 0. 05). Conclusion: Initial periodontal therapy combined with triple therapy can obviously improve the eradication rate of gastric Hp in the patients with gastric ulcer and chronic periodontitis.

Differences between biological characteristics of human periodontal ligament stem cells and human periodontal ligament cells / 中国组织工程研究

BACKGROUND:The biological function of human periodontal ligament stem cells is a hot area of research in the treatment of periodontal disease. Human periodontal ligament cells are one of the end cells derived from human periodontal ligament stem cells;meanwhile, it can also provide supports to the development of human periodontal ligament stem cells. However, few studies are reported about the difference of biological characteristics between human periodontal ligament stem cells and human periodontal ligament cells. OBJECTIVE:To compare the differences of biological characteristics between human periodontal ligament stem cells and human periodontal ligament cells. METHODS:The human periodontal ligament stem cells and human periodontal ligament cells were isolated and purified using tissue explant method and cellclone method, respectively, and then were observed under light microscope to compare the differences of morphology. cellproliferation curves of human periodontal ligament stem cells and human periodontal ligament cells were drawn respectively with cellcounting kit 8 assay. Flow cytometry analysis was used to detect their cellcircles and their surface markers expressions. The alkaline phosphatase gene, proliferating cellnuclear antigen gene and Scleraxis gene of human periodontal ligament stem cells and human periodontal ligament cells were detected by Real-time PCR assay.RESULTS AND CONCLUSION:The human periodontal ligament stem cells and human periodontal ligament cells showed a notable difference in morphology under the light microscope observation. During the first 5 days, the cellproliferation curve of human periodontal ligament stem cells was lower than that of human periodontal ligament cells, but 5 days later, the curve of human periodontal ligament stem cells was significantly higher than that of human periodontal ligament cells. The cellcircles of human periodontal ligament stem cells and human periodontal ligament cells were 41.1%and 23.9%, respectively. The surface markers of human periodontal ligament stem cells and human periodontal ligament cells were similar, but their expression rates had significant difference. The expressions of alkaline phosphatase gene, proliferating cellnuclear antigen gene and Scleraxis gene of human periodontal ligament stem cells were significantly higher than those of human periodontal ligament cells. The above results suggest that human periodontal ligament stem cells have much stronger potential ability than human periodontal ligament cells in osteogensis and cellproliferation.

Acellular dermal matrix allograft combined with coralline hydroxyapatite repair periapical tissue defects / 中国组织工程研究

Chronic periapical periodontitis often causes periapical tissue defects and ultimately leads to the loss of teeth if the inflammation is not promptly cleared to terminate bone resorption and destruction of gingival tissue. Acelular dermal matrix alograft and coraline hydroxyapatite are the common materials to repair periodontal injury. To evaluate clinical efficacy of acelular dermal matrix alograft combined with coraline hydroxyapatite in repairing periapical tissue defects. A total of 76 patients of chronic apical periodontitis were randomly divided into two groups, with 38 cases in each group. In the experimental group, periapical tissue defects were treated with acelular dermal matrix alograft and coraline hydroxyapatite. In the control group, tissue defects were not treated. Al the involved patients underwent apicectomy and retrograde filing. Clinical parameters and radiographic film were recorded at 1 week, 6 months and 3 years folow-up visits to evaluate the repairing effects. After 1 month of treatment, al acelular dermal matrix alografts survived, and the defect of gingival tissues that caused by repairing fistula had been healed. After 3 years, the repairing efficiency in the experimental group was significantly higher than that in the control group (P < 0.05). The bone defect disappeared in the experimental group at 6 months, the transmission of coraline hydroxyapatite particles was decreased, and there were some fuzzy images of compact density. This suggested that new bone was growing. The density of coraline hydroxyapatite particle got closed to normal bone tissue after 3 years, and there were transitional changes of density between coraline hydroxyapatite with normal bone. Coraline hydroxyapatite particle gradualy fused with alveolar bone. Acelular dermal matrix alograft and coraline hydroxyapatite have good biological compatibility. In repairing periapical tissue defects, the application of acelular dermal matrix alograft combined with coraline hydroxyapatite is effective in clinical practice.

Establishment of animal model of rectus abdominis musculoperitoneal flap to reconstruct tongue defect / 华西口腔医学杂志

<p><b>OBJECTIVE</b>This study aims to investigate the feasibility of tongue reconstruction by a rectus abdominis musculoperitoneal flap with neurovascular pedicled in a canine model.</p><p><b>METHODS</b>Twelve Beagle dogs were enrolled to the experiment. The animals were randomly divided into thee groups, two of which (group A and B) had nerve anastomosis. The left sides were experimental sides, whereas the right sides were control sides. Twelve weeks after operation, electrophysiological test was performed to detect hypoglossal nerve latency amplitude and conduction velocity as well as to evaluate the reinnervation of the rectus abdominis musculoperitoneal flap.</p><p><b>RESULTS</b>Among the 12 Beagle dogs, nine animal tongue reconstruction models by rectus abdominis musculoperitoneal flap with neurovascular pedicled were successful, whereas one male Beagle dog died from ventral hemia 3 d after the operation, two female rectus peritoneal flaps were abandoned because their arterial anatomy differed from the male, which was not ideal. Hypoglossal nerve conduction velocity of group A and B were restored to the normal side of the 40%, 30%.</p><p><b>CONCLUSION</b>Animal models of tongue reconstruction can be established by a rectus abdominis musculoperitoneal flap with neurovascular pedicled in Beagle dogs. Denervated rectus abdominis musculoperitoneal flap can regain hypoglossal nerve innervation. Hypoglossal nerve functions partly recover.</p>

Periodontal ligament cells from miniature swine grow on a hydroxyapatite scaffold / 中国组织工程研究

BACKGROUND:Periodontal tissue engineering technology provides new ideas and new ways for periodontitis-induced bone defect repair. OBJECTIVE:To develop a culture model for the periodontal ligament cells of miniature swine, which was constructed with hydroxyapatite, to investigate the biocompatibility with hydroxyapatite. METHODS:Periodontal ligament cells from miniature swine were harvested by using tissue explant method. Immunofluorescence was used to detect the expression of stromal cel antigen 1 in the periodontal ligament cells of miniature swine. The third passage cells were co-cultured with a three-dimension hydroxyapatite scaffold, and the biological characteristics of the cells were observed under a scanning electron microscope at days 1, 3, 7 of co-culture. RESULTS AND CONCLUSION:The pirmary miniature swine periodontal ligament cells grew wel , and they were positive for stromal cel antigen 1. Under the scanning electron microscope, the periodontal ligament cells of miniature swine grew wel on the hydroxyapatite scaffold at days 1, 3, 7 of co-culture. These prove that the miniature swine periodontal ligament cells, which can be separated using tissue explant method and cultured successful y in vitro, can grow wel on the hydroxyapatite scaffold.

An evaluation of the monitor effect of a laser-fluorescent divice during executing the ozone-remineralization treatment to dental caries of deciduous tooth / 实用口腔医学杂志

Traditional atraumatic restorative treatment(ART) induces more successive diseases in the treatment of deciduous caries. Ignoring the disinfection and caries-affected dentin's remineralisation are the main reasons of such problem. Nowadays, Ozone-Remineralisation therapy has become one of the steps before restorative treatment. However, there is no quantitative standard to evaluate this therapy. Some researches had shown that the laser-fluorescent device (DIAGNOdent, Dd) has the advantages in monitoring caries treatment. We use DIAG-NOdent to monitor the effect of OZone-Remineralisation therapy before filling the restoration in the caves in this study, however, the results is delievered that DIAGNOdent has little advatages in monitoring the effect of this new therapy, because the data of DIAGNOdent had a large fluctuation.

The effects of orthodontic force on the expression of IL-6 and the alveolar bone remodeling in rats / 实用口腔医学杂志

Objective; To investigate the expression and distribution of IL-6 in periodontal tissues and the change of the alveolar bone of rats during orthodontic tooth movement, and to study the effects of orthodontic force on the periodontal tissue remodeling. Methods: SO gram orthodontic force was loaded on the left first maxillary molars of 25 rats in experimental group. Immunohistochem-istry and histomorphometric analysis were performed to measure the expression of IL-6 and the loss of alveolar bone at 0, 1, 3, 5, 7 " and 10 days after the application of orthodontic force. Results; The expression of IL-6 was observed to reach maximum level on day 3 and to decline thereafter in experimental group. No obvious alveolar bone loss was detected in the mesial side of the first molars. Conclusion; Although orthodontic force can evoke the local inflammatory response of periodontal tissue and the expression of pro-inflammation cytokines such as IL-6, it can not cause severe periodontal destruction and alveolar bone loss.

Detection of CRP in rat chronic periodontitis and atherosclerosis compound model / 基础医学与临床

Objective To detect and analyse CRP level in rat chronic periodontitis and atherosclerosis compound model.Methods Sixty rats were randomly divided into four groups,15 in each group: Group A:control group;Group B:periodontitis group;Group C:atherosclerosis group;Group D:periodontitis and atherosclerosis group.Every group accepted the corresponding treatment.Pathological changes in periodontal tissue of experimental teeth and arterial vessels were observed.CRP level in serum was assayed by ELISA.Results Histopathological observation of periodontal tissue revealed: Obvious inflammation of periodontal tissue was observed in group B,D.Attachment loss levels in group B and D were higher than that of A,C groups(P

The association of interleukin-1 gene polymorphisms with the susceptibility to chronic periodontitis in Uighur / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To investigate the relationship between interleukin-1 (IL-1) gene polymorphisms and the susceptibility of chronic periodontitis in Uighur minority in Xingjiang province of China.</p><p><b>METHODS</b>The buccal swabs were collected from 41 severe chronic periodontitis (CP) patients, 43 moderate CP patients, 49 mild CP patients and 92 healthy controls. DNA was extracted from these buccal swabs. Genotypes of the IL-1A-889/NcoI and IL-1B+3954/TaqI were determined by sequence specific primers-polymerase chain reaction(SSP-PCR) and PCR-restriction fragment length polymorphism(PCR-RFLP). Then distribution of genotypes for IL-1A-889 and IL-1B+3954 were compared among the different groups.</p><p><b>RESULTS</b>(1) There were no significant differences in the distribution of IL-1A-889 among severe CP patients, moderate CP patients, mild CP patients and healthy controls. (2) Frequencies of allele 2 for IL-1B+3954 were higher in severe CP patients than in healthy controls, and the difference was statistically significant. But there were no such significant differences either between moderate CP patients and healthy controls or between mild CP patients and healthy controls.</p><p><b>CONCLUSION</b>These results suggest that IL-1B+3953 allele 2 may be a risk indicator for the susceptibility to severe chronic periodontitis in Uighur minority in Xingjiang of China.</p>