TmNAS3 from Triticum monococum directly regulated by TmbHLH47 increases Fe content of wheat grain.

Biofortification is an effective way to enhance wheat grain Fe content. However, Fe overload inhibits the growth and development of wheat. In this work, the impact of Triticum monococcum nicotianamine synthase 3 (TmNAS3) on Fe accumulation in wheat grain was analyzed. Transgenic wheat expressing TmNAS3 was obtained via Agrobacterium-mediated transformation. The concentrations of Fe in the grains of two transgenic wheat lines were 62.42 µg/g and 68.75 µg/g, while that in the non-transgenic line (NT) was only 29.51 µg/g. Exogenous Fe application induced the expression of natural resistance-associated macrophage protein 3 (NRAMP3), NRAMP6, yellow stripe-like protein 3 (YSL3), YSL6, and vacuolar iron transporter 2 in transgenic wheat. The transcription factor that bound to the TmNAS3 promoter was identified, and the findings suggested that TmbHLH47 directly interacted and promoted the transcription of TmNAS3. Moreover, TmbHLH47 was observed to bind directly to the G-box in TmNAS3 promoter and regulated the transcriptional level of TmNAS3. Our findings contribute a TmbHLH47/TmNAS3 transcriptional pathway and thereby provide a potential strategy for improving the Fe concentration of wheat through genetic engineering.

AetSRG1 contributes to the inhibition of wheat Cd accumulation by stabilizing phenylalanine ammonia lyase.

Cadmium (Cd) is a toxic heavy metal that poses a serious threat to crop safety, productivity, and human health. Aegilops tauschii is the D genome donor of common wheat and shows abundant genetic variation. However, the tolerance of Ae. tauschii toward Cd at the molecular level is poorly understood. In this study, key factors involved in the Cd stress response of Ae. tauschii were investigated by RNA sequencing. Differentially expressed genes (DEGs) under Cd stress were identified in Ae. tauschii roots and shoots. A Fe(II)/2-oxoglutarate dependent dioxygenase (designated as AetSRG1), with an unknown function in Cd stress, was of particular interest. The open reading frame of AetSRG1 was cloned and overexpressed in wheat, which resulted in reduced Cd accumulation along with a lower Cd2+ flux, decreased electrolyte leakage, and higher reactive oxygen species production. The protein of AetSRG1 interacted with phenylalanine ammonia lyase (PAL). Finally, we found that AetSRG1 stabilizes PAL and promotes the synthesis of endogenous salicylic acid. This study provides novel insights into the molecular mechanisms underlying the response of Ae. tauschii toward Cd stress. The key genes identified in this work serve as potential targets for developing low cadmium wheat.

Applications of Polydopamine-Modified Scaffolds in the Peripheral Nerve Tissue Engineering.

Peripheral nerve injury is a common and complicated traumatic disease in clinical neurosurgery. With the rapid advancement and development of medical technologies, novel tissue engineering provides alternative therapies such as nerve conduit transplantation. It has achieved significant outcomes. The scaffold surface modification is vital to the reconstruction of a pro-healing interface. Polydopamine has high chemical activity, adhesion, hydrophilicity, hygroscopicity, stability, biocompatibility, and other properties. It is often used in the surface modification of biomaterials, especially in the peripheral nerve regeneration. The present review discusses that polydopamine can promote the adhesion, proliferation, and differentiation of neural stem cells and the growth of neuronal processes. Polydopamine is widely used in the surface modification of nerve conduits and has a potential application prospect of repairing peripheral nerve injury. Polydopamine-modified scaffolds are promising in the peripheral nerve tissue engineering.