Characterization of the Bujaru, frijoles and Tapara antigenic complexes into the sandfly fever group and two unclassified phleboviruses from Brazil.

The genus Phlebovirus includes the sandfly fever viruses and tick-transmitted uukuviruses. Sandfly fever group viruses have been isolated from various vertebrate species and from phlebotomines and occasionally alternative arthropods, e.g. mosquitoes, or ceratopogonids of the genus Culicoides. Uukuniemi serogroup viruses have been isolated from various vertebrate species and from ticks. Despite the public health importance of some viruses of the genus, the genomic diversity of phleboviruses that could be incriminated as causative of human or veterinary diseases remains underestimated. Here we describe the nearly complete sequences and genomic characterization of two phleboviruses belonging to the Bujaru antigenic complex: the prototype species and the Munguba virus. Furthermore, six previously unclassified phleboviruses isolated in Brazil were also sequenced and characterized: Ambe, Anhanga, Joa, Uriurana, Urucuri and Tapara viruses. The results of the phylogenetic analysis indicated that these viruses group with viruses of three antigenic complexes (Bujaru, Tapara and frijoles clades), with two unclassified phleboviruses. We also performed genomic reassortment analysis and confirmed that there were no events for the viruses described in this study, but we found a new potential reassortment in Medjerda Valley virus, which contains S and L segments of Arbia virus, and probably a unique M segment, both viruses circulate in the same geographic region, indicating these two isolates represent two distinct viruses. This study provides insights into the genetic diversity, classification and evolution of phleboviruses.

Complete genome sequence of human T-cell lymphotropic type 1 from patients with different clinical profiles, including infective dermatitis.

The HTLV-1 is the first human retrovirus and is associated with several clinical syndromes, however, the pathogenesis of these clinical manifestations is still not fully understood. Furthermore, there are few complete genomes publicly available, about 0.12 complete genomes per 10,000 infected individuals and the databases have a major deficiency of sequences information. This study generated and characterized 31 HTLV-1 complete genomes sequences derived from individuals with Tropical Spastic Paraparesis/HTLV-1-Associated Myelopathy (TSP/HAM), Adult T-cell leukemia/lymphoma (ATL), infective dermatitis associated to HTLV-1 (IDH) and asymptomatic patients. These sequences are associated to clinical and epidemiological information about the patients. The sequencing data generated on Ion Torrent PGM platform were assembled and mapped against the reference HTLV-1 genome. These sequences were genotyped as Cosmopolitan subtype, Transcontinental subgroup. We identified the variants in the coding regions of the genome of the different clinical profiles, however, no statistical relation was detected. This study contributed to increase of HTLV-1 complete genomes in the world. Furthermore, to better investigate the contribution of HTLV-1 mutations for the disease outcome it is necessary to evaluate the interaction of the viral genome and characteristics of the human host.

Characterization of mitochondrial genome of Haemagogus janthinomys (Diptera: Culicidae).

Haemagogus janthinomys is a mosquito of high importance in public health due its involvement on natural wild cycles of two important arboviruses in the Brazilian Amazon region: Yellow Fever virus (Flaviviridae, Flavivirus) and Mayaro virus (Togaviridae, Alphavirus). Here, we have sequenced and described all the mitochondrial genes for the Hg. janthinomys species. The complete coding sequence is14 937 bp long and includes 37 functional genes, of which 13 codes for proteins, 22 for tRNA and 2 for ribosomal subunits. Region A + T (control region) is not presented here. The data should be helpful on further taxonomic and evolutionary studies of this important arbovirus vector.

Polymorphism of DC-SIGN (CD209) promoter in association with clinical symptoms of dengue fever.

C-type lectin DC-SIGN receptor, encoded by CD209, plays a key role in the infection of dendritic cells by dengue virus (DENV). Because the -336A/G SNP (rs4804803) polymorphism in the promoter of CD209 modulates DC-SIGN expression, we investigated the putative association of this polymorphism with DENV infection and its pathogenesis. A control sample of 72 individuals, rigorously selected through a clinical investigation for absence of past dengue fever (DF) was compared to a sample of 168 patients (156 classical DF; 12 dengue hemorrhagic fever), all residents from Pará, Brazil. However, the prevalence of symptoms showed a trend higher in the AA genotype (Wilcoxon test; Z=2.02; p=0.04). Hence, our findings indicate that the G allele downregulates the spectrum of symptoms during the early acute phase of DENV infection, putatively decreasing the viremia, as suggested in the literature.

Descrição da anatomia radiográfica do crânio do Cebus Apella (Linnaeus, 1758) / Anatomic-radiographic description of cebus apella (linnaeus, 1758) skull

Introdução: as semelhanças filogenéticas entre primatas não humanos e seres humanos estimulam estudos de seu sistema estomatognático, visando seu uso em pesquisas. Objetivo: descrever as estruturas anatômicas da maxila e da mandíbula do Cebus apella, comparando às características humanas. Material e Método: foram utilizados dois animais adultos. Após a remoção do tecido orgânico, os crânios e maxilas foram radiografados e fotografados, sendo analisados com a lupa. Resultado e discussão: as estruturas anatômicas da maxila e da mandíbula apresentavam características semelhantes ao ser humano, mas com algumas peculiaridades, tais como: a presença do terceiro pré-molar; proeminências caninas evidentes em ambos os arcos; a colocação mental do majestoso; largura do ramo ascendente do maxilar; a presença do forame mandibular; Formato V do maxilar; o tamanho do forame incisivo; presença da sutura incisiva. Com relção à interpretação radiográfica da câmara pulpar e canal radicular desta espécie, os dentes incisivos central e lateral superiores, caninos superiores e inferiores e 1º, 2º, 3º Pré-molares inferiores são dentes com canais únicos, amplos, de fácil acesso e, desse modo, ideais para experimentos endodônticos. Conclusão: o Cebus apella pode ser usado como modelo de estudo em tratamento endodôntico.

Introduction: the phylogenetic similarities between non-human primates and humans stimulate studies of their stomatognathic system, aiming their use in research. Objective: the objective of this study was to describe the anatomical structures of the maxilla and mandible of Cebus apella comparing to human characteristics. Material and Method: two adult animals were used. After removing the organic tissue, the skulls and jaws were x-rayed and photographed, being analyzed with the magnifying glass. Results: the results showed that the anatomical structures of the maxilla and the mandible had similar characteristics to the human being, but with some peculiarities, such as: the presence of the third premolar; prominent canine prominences in both arches; the mental setting of the majestic; width of ascending branch of maxilla; the presence of the mandibular foramen; V shape of the jaw; incisor foramen size; presence of the incisive suture. With respect to the radiographic interpretation of the pulp chamber and root canal of this species, the maxillary and mandibular maxillary teeth, maxillary and mandibular maxillary teeth are teeth with single, wide, easily accessible ideal for endodontic experiments. Conclusion: thus, the authors conclude that Cebus apella can be used as a study model in endodontic treatment.

Comparação da resistência ao cisalhamento após colagem com dois cimentos ortodônticos ­ estudo in vitro / A comparison of shear bond strength after bonding with two orthodontic cements ­ an in vitro study

O objetivo deste trabalho foi avaliar a resistência ao cisalhamento de bandas ortodônticas cimentadas com um novo compósito Orthobite, comparando-as ao cimento ionômero de vidro convencional em esmalte bovino e analisar o Índice de Remanescente Adesivo (IRA). A amostra consistiu de 30 dentes bovinos divididos em 2 grupos: Grupo I ­ Orthobite (FGM) e Grupo II ­ CIV MERON (VOCO). Foi realizada profilaxia com pedra-pomes e água, seguida da cimentação conforme instruções dos fabricantes. Logo após, os mesmos foram submetidos ao ensaio de cisalhamento com velocidade de 0,5 mm/minutos. Os dados foram submetidos aos testes de T Student e Kruskal-Wallis. Foram encontradas diferenças estatísticas significantes entre os grupos, tanto nos valores de resistência ao cisalhamento (p>0,05), quanto na avaliação do IRA (p>0,05). O cimento Orthobite mostrou-se estatisticamente superior ao MERON, ou seja, apresentou um melhor vedamento das margens e uma maior resistência ao deslocamento, dessa forma essa união estável entre o dente e a banda é fundamental para o sucesso do tratamento ortodôntico (AU)

The objective of this study was to evaluate the shear strength of orthodontic bands cemented with a new Orthobite composite, comparing them to the conventional glass ionomer cement in bovine enamel and to analyze the Adhesive Remnant Index (ARI). The sample consisted of 30 bovine teeth divided into 2 groups: Group I ­ Orthobite (FGM) and Group II ­ CIV MERON (VOCO). Prophylaxis has been made with pumice and water followed by cementation according to the manufacturer's instructions. Afterwards, they were subjected to the shear test at a speed of 0.5 mm/minute speed. Data were submitted to the Student T and Kruskal-Wallis tests. Significant statistical differences were found between the groups both in the shear strength values (p> 0.05) and in the ARI assessment (p> 0.05). The Orthobite cement proved to be statistically superior to MERON, that is, it presented a better margins seal and a greater resistance to displacement, so this stable union between the tooth and the band is fundamental for the success of the orthodontic treatment. (AU)