RF properties of circular-ranging OCT signals.

Circular-ranging optical coherence tomography (CR-OCT) systems that use a time-stepped frequency comb source generate interference fringe signals that are more complex than those of a conventional swept-source OCT system. Here, we define a common terminology for describing these signals, and we develop a mathematical framework that relates the radio-frequency (RF) properties of these fringe signals to the parameters of the frequency comb source. With this framework, we highlight non-intuitive mechanisms whereby the design of the frequency comb source can affect imaging performance. We show, for example, that amplitude-pulsed time-stepped frequency comb sources have a sensitivity advantage over constant power time-stepped frequency comb sources. More broadly, this framework and associated terminology provide a foundation on which to design and optimize time-stepped frequency comb sources and systems.

Spectral- and Polarization-Dependent Scattering of Gold Nanobipyramids for Exogenous Contrast in Optical Coherence Tomography.

Polarization-sensitive optical coherence tomography (PS-OCT) reveals the subsurface microstructure of biological tissue and provides information regarding the polarization state of light backscattered from tissue. Complementing OCT's structural signal with molecular imaging requires strategies to simultaneously detect multiple exogenous contrast agents with high specificity in tissue. Specific detection of molecular probes enables the parallel visualization of physiological, cellular, and molecular processes. Here we demonstrate that, by combining PS-OCT and spectral contrast (SC)-OCT measurements, we can distinguish signatures of different gold nanobipyramids (GNBPs) in lymphatic vessels from the surrounding tissue and blood vessels in live mouse models. This technique could well be extended to other anisotropic nanoparticle-based OCT contrast agents and presents significant progress toward enabling OCT molecular imaging.

Resolving absolute depth in circular-ranging optical coherence tomography by using a degenerate frequency comb.

In Fourier-domain optical coherence tomography, an interference signal is generated that spans an RF bandwidth proportional to the product of three parameters: the imaging range, the imaging speed, and the inverse of the axial resolution. Circular-ranging optical coherence tomography (CR-OCT)architectures were introduced to ease long-range imaging by decoupling the imaging range from the signal RF bandwidth. As a consequence, present CR-OCT systems resolve the relative, but not the absolute, depth location of the scatterers. We introduce here a modified implementation of CR-OCT that uses a degenerate frequency comb source that allows recovery of absolute depth information while only minimally impacting the previously described RF bandwidth compression benefits of CR. We show that this degenerate frequency comb can be created by relatively simple modifications to existing frequency comb source designs, and we present absolute ranging capabilities through imaging studies and simulations.

Resolving absolute depth in circular-ranging optical coherence tomography by using a degenerate frequency comb: publisher's note.

This publisher's note contains corrections to Opt. Lett.45, 371 (2020).OPLEDP0146-959210.1364/OL.379968.

Extended bandwidth wavelength swept laser source for high resolution optical frequency domain imaging.

Improving the axial resolution by providing wider bandwidth wavelength swept lasers remains an important issue for optical frequency domain imaging (OFDI). Here, we demonstrate a wide tuning range, all-fiber wavelength swept laser at a center wavelength of 1250 nm by combining two ring cavities that share a single Fabry-Perot tunable filter. The two cavities contain semiconductor optical amplifiers with central wavelengths of 1190 nm and 1292 nm, respectively. To avoid disturbing interference effects in the overlapping spectral region, we modulated the amplifiers in order to obtain consecutive wavelength sweeps in the two spectral regions. The two sweeps were fused together in post-processing to achieve a total scanning range of 223 nm, corresponding to 3.3 µm axial resolution in air. We confirm improved image quality and reduced speckle size in tomograms of swine esophagus ex vivo, and human skin and nailbed in vivo.

Degree of polarization (uniformity) and depolarization index: unambiguous depolarization contrast for optical coherence tomography.

The degree of polarization (uniformity) has attracted increased interest as a functional contrast in optical coherence tomography (OCT). However, its computation from a single polarization state suggests an ambiguity that is strongly dependent on a sample's orientation. We here propose an improved metric to present depolarization with respect to the optical system rather than the propagating field. Using numerical simulations and optical frequency domain imaging, we evaluate the conventional DOP(U) for different polarization states and compare its performance with the unambiguous depolarization index.

Single input state, single-mode fiber-based polarization-sensitive optical frequency domain imaging by eigenpolarization referencing.

Fiber-based polarization-sensitive optical frequency domain imaging is more challenging than free-space implementations. Using multiple input states, fiber-based systems provide sample birefringence information with the benefit of a flexible sample arm but come at the cost of increased system and acquisition complexity, and either reduce acquisition speed or require increased acquisition bandwidth. Here we show that with the calibration of a single polarization state, fiber-based configurations can approach the conceptual simplicity of traditional free-space configurations. We remotely control the polarization state of the light incident at the sample using the eigenpolarization states of a wave plate as a reference, and determine the Jones matrix of the output fiber. We demonstrate this method for polarization-sensitive imaging of biological samples.

Single-shot speckle reduction and dispersion compensation in optical coherence tomography by compounding fractional Fourier domains.

We present speckle suppression and dispersion compensation for Fourier-domain optical coherence tomography based on fractional Fourier transforms of a single A scan. A 1.54-fold reduction in speckle contrast was achieved with group velocity dispersion compensation. The method is demonstrated on biological samples using a swept source configuration at 1310 nm and a spectral-domain system at 840 nm.

Dispersion mapping at the micrometer scale using tri-band optical frequency domain imaging.

Techniques to differentiate between materials are a powerful addition to the structural information traditionally available from optical coherence tomography images. We present label-free detection of water and lipid at a micrometer scale by evaluating their unique dispersion properties. Using a tri-band swept source configuration, we measure both ß(2) and ß(3) and show how to identify the two materials at sample thicknesses of 40 and 90 µm, respectively.

Dispersion compensation in Fourier domain optical coherence tomography using the fractional Fourier transform.

We address numerical dispersion compensation based on the use of the fractional Fourier transform (FrFT). The FrFT provides a new fundamental perspective on the nature and role of group-velocity dispersion in Fourier domain OCT. The dispersion induced by a 26 mm long water cell was compensated for a spectral bandwidth of 110 nm, allowing the theoretical axial resolution in air of 3.6 µm to be recovered from the dispersion degraded point spread function. Additionally, we present a new approach for depth dependent dispersion compensation based on numerical simulations. Finally, we show how the optimized fractional Fourier transform order parameter can be used to extract the group velocity dispersion coefficient of a material.

Instantaneous quadrature components or Jones vector retrieval using the Pancharatnam-Berry phase in frequency domain low-coherence interferometry.

We use the Pancharatnam-Berry phase as a multifunctional tool for low-coherence interferometry. This geometric phase shift enables instantaneous retrieval of the quadrature components of the complex interferometric signal. The phase shift is independent of wavelength and allows for a complex conjugate suppression of 40 dB for an optical bandwidth of 115 nm. Furthermore, this paper investigates the versatility of the geometric phase to perform polarization sensitive measurements. The Jones vector of the sample was obtained numerically, allowing sample birefringence and optical axis calculation.

Nanosecond SRS fiber amplifier for label-free near-infrared photoacoustic microscopy of lipids.

Near-infrared photoacoustics receives increasing interest as an intravital modality to sense key biomolecules. One of the most central types of biomolecules of interest are lipids as they constitute essential bio-hallmarks of cardiovascular and metabolic diseases and their in-vivo detection holds insightful information about disease progression and treatment monitoring. However, the full potential of near-infrared photoacoustic for high-resolution and high-sensitivity biomedical studies of lipids has so far not been exploited due a lack of appropriate excitation sources delivering short-pulses at high-repetition-rate, high-pulse-energy, and wavelength around 1200 nm. Here, we demonstrate a custom-built SRS fiber amplifier that provides optical excitations at 1192.8 nm, repetition rates of 200 kHz, pulse durations below 2 ns, and pulse energies beyond 5 µJ. We capitalize on the performance of our excitation source and show near-infrared photoacoustics resolving intrinsic lipid contrast in biomedically relevant specimens ranging from single cells to lipid-rich tissue with subcellular resolution.

Improving the efficiency of optical coherence tomography by using the non-ideal behaviour of a polarising beam splitter.

We present a new way of improving the efficiency of optical coherence tomography by using the polarisation crosstalk of a polarising beam splitter to direct most of the available source optical power to the sample. The use of a quarter wave plate in both the reference and the sample arms allows most of the sample power to be directed to the detector while adjusting the reference arm to ensure noise optimised operation. As a result, the sensitivity of such a system can be improved by 6 dB, or alternatively the acquisition time can be improved by a factor of 4 for shot noise limited performance,compared to a traditional OCT configuration using a 50/50 beam splitter.

Multi-beam OCT imaging based on an integrated, free-space interferometer.

While it is a common practice to increase the speed of swept-source optical coherence tomography (OCT) systems by using a high-speed source, this approach may not always be optimal. Parallelization in the form of multiple imaging beams is an alternative approach, but scalable and low-loss multi-beam OCT architectures are needed to capitalize on its advantages. In this study, we demonstrate an eight-beam OCT system using an interferometer architecture comprising planar lightwave circuits (PLC) splitters, V-groove assemblies (VGA), and optical ribbon fibers. We achieved an excess loss and heterodyne efficiency on each channel that was close to that of single-beam systems. In vivo structural imaging of a human finger and OCT angiography imaging of a mouse ear was performed to demonstrate the imaging performance of the system. This work provides further evidence supporting multi-beam architectures as a viable strategy for increasing OCT imaging speed.

Stable multi-megahertz circular-ranging optical coherence tomography at 1.3 µm.

In Fourier-domain optical coherence tomography (OCT), the finite bandwidth of the acquisition electronics constrains the depth range and speed of the system. Circular-ranging (CR) OCT methods use optical-domain compression to surpass this limit. However, the CR-OCT system architectures of prior reports were limited by poor stability and were confined to the 1.55 µm wavelength range. In this work, we describe a novel CR-OCT architecture that is free from these limitations. To ensure stable operation, temperature sensitive optical modules within the system were replaced; the kilometer-length fiber spools used in the stretched-pulse mode-locked (SPML) laser was eliminated in favor of a single 10 meter, continuously chirped fiber Bragg grating, and the interferometer's passive optical quadrature demodulation circuit was replaced by an active technique using a lithium niobate phase modulator. For improved imaging penetration in biological tissues, the system operating wavelength was shifted to a center wavelength of 1.29 µm by leveraging the wavelength flexibility intrinsic to CFBG-based dispersive fibers. These improvements were achieved while maintaining a broad (100 nm) optical bandwidth, a long 4 cm imaging range, and a high 7.6 MHz A-line rate. By enhancing stability, simplifying overall system design, and operating at 1.3 µm, this CR-OCT architecture will allow a broader exploration of CR-OCT in both medical and non-medical applications.

Intravascular Polarimetry in Patients With Coronary Artery Disease.

OBJECTIVES: The aims of this first-in-human pilot study of intravascular polarimetry were to investigate polarization properties of coronary plaques in patients and to examine the relationship of these features with established structural characteristics available to conventional optical frequency domain imaging (OFDI) and with clinical presentation. BACKGROUND: Polarization-sensitive OFDI measures birefringence and depolarization of tissue together with conventional cross-sectional optical frequency domain images of subsurface microstructure. METHODS: Thirty patients undergoing polarization-sensitive OFDI (acute coronary syndrome, n = 12; stable angina pectoris, n = 18) participated in this study. Three hundred forty-two cross-sectional images evenly distributed along all imaged coronary arteries were classified into 1 of 7 plaque categories according to conventional OFDI. Polarization features averaged over the entire intimal area of each cross section were compared among plaque types and with structural parameters. Furthermore, the polarization properties in cross sections (n = 244) of the fibrous caps of acute coronary syndrome and stable angina pectoris culprit lesions were assessed and compared with structural features using a generalized linear model. RESULTS: The median birefringence and depolarization showed statistically significant differences among plaque types (p < 0.001 for both, one-way analysis of variance). Depolarization differed significantly among individual plaque types (p < 0.05), except between normal arteries and fibrous plaques and between fibrofatty and fibrocalcified plaques. Caps of acute coronary syndrome lesions and ruptured caps exhibited lower birefringence than caps of stable angina pectoris lesions (p < 0.01). In addition to clinical presentation, cap birefringence was also associated with macrophage accumulation as assessed using normalized SD. CONCLUSIONS: Intravascular polarimetry provides quantitative metrics that help characterize coronary arterial tissues and may offer refined insight into coronary arterial atherosclerotic lesions in patients.

Extended Coherence Length and Depth Ranging Using a Fourier-Domain Mode-Locked Frequency Comb and Circular Interferometric Ranging.

Fourier domain mode-locking (FDML) has been a popular laser design for high speed optical frequency domain imaging (OFDI) but the achievable coherence length, and therefore imaging range, has been limited. The narrow instantaneous linewidth of a frequency comb (FC) FDML laser could provide an attractive platform for high speed as well as long range OFDI. Unfortunately, aliasing artifacts arising from signals beyond the principle measurement depth of the free spectral range have prohibited the use of a FC FDML for imaging so far. To make the enhanced coherence length of FC FDML laser available, methods to manage such artifacts are required. Recently, coherent circular ranging has been demonstrated that uses frequency combs for imaging in much reduced RF bandwidths. Here, we revisit circular ranging as a tool of making the long coherence length of an FDML frequency comb laser as well as its use for tissue imaging accessible. Using an acousto-optic frequency shifter (AOFS), we describe an active method to mitigate signal aliasing that is both stable and wavelength independent. We show that an FC FDML laser offers an order of magnitude improved coherence length compared to traditional FDML laser designs without requiring precise dispersion engineering. We discuss design parameters of a frequency stepping laser resonator as well as aliasing from a frequency comb and AOFS in OFDI with numerical simulations. The use of circular ranging additionally reduced acquisition bandwidths 15-fold compared with traditional OFDI methods. The FC FDML/AOFS design offers a convenient platform for long range and high speed imaging as well as exploring signal and image processing methods in circular ranging.

Quantitative depolarization measurements for fiber-based polarization-sensitive optical frequency domain imaging of the retinal pigment epithelium.

A full quantitative evaluation of the depolarization of light may serve to assess concentrations of depolarizing particles in the retinal pigment epithelium and to investigate their role in retinal diseases in the human eye. Optical coherence tomography and optical frequency domain imaging use spatial incoherent averaging to compute depolarization. Depolarization depends on accurate measurements of the polarization states at the receiver but also on the polarization state incident upon and within the tissue. Neglecting this dependence can result in artifacts and renders depolarization measurements vulnerable to birefringence in the system and in the sample. In this work, we discuss the challenges associated with using a single input polarization state and traditional depolarization metrics such as the degree-of-polarization and depolarization power. We demonstrate quantitative depolarization measurements based on Jones vector synthesis and polar decomposition using fiber-based polarization-sensitive optical frequency domain imaging of the retinal pigment epithelium in a human eye.

Optic axis mapping with catheter-based polarization-sensitive optical coherence tomography.

Birefringence offers an intrinsic contrast mechanism related to the microstructure and arrangement of fibrillary tissue components. Here we present a reconstruction strategy to recover not only the scalar amount of birefringence but also its optic axis orientation as a function of depth in tissue from measurements with catheter-based polarization sensitive optical coherence tomography. A polarization symmetry constraint, intrinsic to imaging in the backscatter direction, facilitates the required compensation for wavelength-dependent transmission through system elements, the rotating catheter, and overlying tissue layers. Applied to intravascular imaging of coronary atherosclerosis in human patients, the optic axis affords refined interpretation of plaque architecture.

High-speed optical coherence tomography by circular interferometric ranging.

Existing three-dimensional optical imaging methods excel in controlled environments but are difficult to deploy over large, irregular and dynamic fields. This has limited imaging in areas such as material inspection and medicine. To better address these applications, we developed methods in optical coherence tomography (OCT) to efficiently interrogate sparse scattering fields, i.e., those in which most locations (voxels) do not generate meaningful signal. Frequency comb sources are used to superimpose reflected signals from equispaced locations through optical subsampling. This results in circular ranging, and reduces the number of measurements required to interrogate large volumetric fields. As a result, signal acquisition barriers that have limited speed and field in OCT are avoided. With a new ultrafast, time-stretched frequency comb laser design operating with 7.6 MHz to 18.9 MHz repetition rates, we achieved imaging of multi-cm3 fields at up to 7.5 volumes per second.