RESUMO
Impact of insect pests makes a significant limitation of the sugar beet crop yield. Integration of cry-genes of Bacillus thuringiensis into plant genome is one of the promising strategies to ensure plant resistance. The aim of this work was to obtain sugar beet lines (based on the MM 1/2 line) transformed with cry2A and cry1Cgenes. We have optimized transformation protocol and direct plant let regeneration protocol from leaf explants using 1 mg/l benzylaminopurine as well as 0,25 mg/l benzylaminopurine and 0,1 mg/l indole-butyric acid. Consequently, transgenic sugar beet lines transformed with vector constructs pRD400-cry1C and pRD400-cry2A have been obtained. PCR analysis revealed integration of cry2A and cry1C into genome of transgenic lines and expression of these genes in leaf tissues was shown by reverse transcription PCR.
Assuntos
Proteínas de Bactérias/genética , Beta vulgaris/genética , Dípteros/patogenicidade , Endotoxinas/genética , Proteínas Hemolisinas/genética , Controle Biológico de Vetores , Doenças das Plantas/genética , Folhas de Planta/genética , Animais , Bacillus thuringiensis/química , Bacillus thuringiensis/genética , Toxinas de Bacillus thuringiensis , Proteínas de Bactérias/metabolismo , Compostos de Benzil/farmacologia , Beta vulgaris/efeitos dos fármacos , Beta vulgaris/imunologia , Beta vulgaris/parasitologia , Dípteros/fisiologia , Endotoxinas/metabolismo , Expressão Gênica , Técnicas de Transferência de Genes , Vetores Genéticos , Genoma de Planta , Proteínas Hemolisinas/metabolismo , Indóis/farmacologia , Doenças das Plantas/imunologia , Doenças das Plantas/parasitologia , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/imunologia , Folhas de Planta/parasitologia , Plantas Geneticamente Modificadas , Purinas/farmacologia , TransgenesRESUMO
Shown antimicrotrubules activity of new 2,6-dinitroaniline compounds. Investigated their ability on apoptotic processes in a plant cell when used as a composition with inhibitors of acetyl-CoA carboxylase.