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1.
Tour Manag ; 88: 104416, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34629610

RESUMO

With the tourism and hospitality sector reopening post-lockdown of COVID-19, the recovery of customers' purchase intentions is essential to reboot the sector. This study aims to examine the relationship between social distancing measures and purchase intentions in the UK's restaurant and hotel sectors using a propensity score weighting experimental design method. The findings suggest that the impact of social distancing measures on purchase intentions is mediated by the trust in the targeted restaurant and hotel. Risk tolerance significantly moderates the influence of social distancing measures on trust; (non-) cash promotions have an insignificant impact on purchase intentions. The introduction of the propensity score weighting scheme addresses the endogeneity caused by the sampling bias in non-probability sampling experiment studies.

2.
Tour Manag ; 91: 104525, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35308799

RESUMO

The COVID-19 pandemic has brought sweeping changes to global tourism alongside large-scale travel restrictions, posing complex challenges to entrepreneurs and firms seeking to find their footing in a turbulent climate. This study presents a theoretical framework linking uncertainty, capital, and innovation to analyse how bed-and-breakfast small and medium-sized enterprises have innovatively responded to unprecedented obstacles during COVID-19 recovery. Three-stage longitudinal interviews were conducted with more than 30 entrepreneurs between April and November 2020 to unpack their ongoing responses to the pandemic. The recovery process was found to be non-linear due to the shifting nature of sources of uncertainty and changes in entrepreneurs' capital. These alterations shaped interviewees' responses, especially in terms of product and marketing innovations, which ultimately generated new uncertainty.

3.
Zhongguo Zhong Yao Za Zhi ; 47(18): 4908-4918, 2022 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-36164900

RESUMO

With prominent medicinal value, Gelsemium elegans has been overexploited, resulting in the reduction of the wild resource. As a result, artificial cultivation turns out to be a solution. However, this medicinal species is intolerant to low temperature, and thus genes responding to the low temperature are important for the cultivation of this species. Based on the transcriptome database of G. elegans at 4 ℃, 29 differentially expressed GeERF genes were identified. Bioinformatics analysis of 21 GeERF gene sequences with intact open reading frames showed that 12 and 9 of the GeERF proteins respectively clustered in DREB subgroup and ERF subgroup. GeDREB1 A-1-GeERF6 B-1, with molecular weight of 23.78-50.96 kDa and length of 212-459 aa, were all predicted to be hydrophilic and in nucleus. Furthermore, the full-length cDNA sequence of GeERF2B-1 was cloned from the leaves of G. elegans. Subcellular localization suggested that GeERF2B-1 was located in the nucleus. According to the quantitative reverse-transcription PCR(qRT-PCR), GeERF2B-1 showed constitutive expression in roots, stems, and leaves of G. elegans, and the expression was the highest in roots. In terms of the response to 4 ℃ treatment, the expression of GeERF2B-1 was significantly higher than that in the control and peaked at 12 h, suggesting a positive response to low temperature. This study lays a scientific basis for the functional study of GeERF transcription factors and provides gene resources for the improvement of stress resistance of G. elegans.


Assuntos
Regulação da Expressão Gênica de Plantas , Fatores de Transcrição , DNA Complementar , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Temperatura , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
5.
Gene ; 822: 146331, 2022 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-35183686

RESUMO

Silicon, one of the most prevalent elements in the soil, is beneficial for plant growth and defense against different stresses. The silicon transporter gene (Lsi) plays an important role in the uptake and transport of silicon in higher plants. In this study, a total of 32 Lsi genes, including 20 SsLsi in sugarcane wild species Saccharum spontaneum, 5 ShLsi in Saccharum hybrid cultivar R570 and 7 SbLsi in sugarcane related species Sorghum bicolor, were identified and classified into three groups. Bioinformatics analysis showed that instability, hydrophobicity, localization of cell membranes and vacuoles were the main features of the Lsi proteins. Whole genome and segmental duplication contributed to the main expansion of Lsi gene family. Collinearity analysis of the Lsi genes showed that S. spontanum and R570 had a collinear relationship with monocotyledonous plants S. bicolor and Oryza sativa, but not with dicotyledonous plants Arabidopsis thaliana and Vitis vinifera. The replicated Lsi genes were mainly subjected to strong selection pressure for purification. The diverse cis-regulatory elements in the promoter of SsLsi, ShLsi and SbLsi genes suggested that they were widely involved in the response of plants to various stresses and the regulation of the growth and development. Transcriptome data and real time quantitative PCR analysis showed that the Lsi genes exhibited different expression profiles in sugarcane tissues and under Sporisorium scitamineum, drought and cold stresses. In addition, the cDNA and genomic DNA sequences of ShLsi6 that was homologous to SsLsi1b gene was cloned from Saccharum hybrid cultivar ROC22. Transient expression analysis showed that, compared with the control, Nicotiana benthamiana leaves which overexpressed the ShLsi6 gene showed a high sensitivity after inoculation with tobacco pathogens Ralstonia solanacearum and Fusarium solani var. coeruleum. This study provides important information for further functional analysis of Lsi genes and resistant breeding in sugarcane.


Assuntos
Proteínas de Transporte/genética , Clonagem Molecular/métodos , Biologia Computacional/métodos , Saccharum/crescimento & desenvolvimento , Proteínas de Transporte/química , Proteínas de Transporte/metabolismo , Mapeamento Cromossômico , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Família Multigênica , Proteínas de Plantas/química , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Saccharum/classificação , Saccharum/genética , Saccharum/metabolismo , Análise de Sequência de DNA , Silício/metabolismo , Estresse Fisiológico , Distribuição Tecidual
6.
Ann Tour Res ; 91: 103298, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34518712

RESUMO

This study aims to investigate the contribution of aviation related travel restrictions to control the spread of COVID-19 in Europe by using quasi-experiment approaches including the regression discontinuity design and a two-stage spatial Durbin model with an instrumental variable. The study provides concrete evidence that the severe curtailing of flights had a spontaneous impact in controlling the spread of COVID-19. The counterfactual analysis encapsulated the spillover effects deduced that a 1% decrease in flight frequency can decrease the number of confirmed cases by 0.908%. The study also reveals that during the lockdown, the aviation industry cancelled over 795,000 flights, which resulted in averting an additional six million people being from being infected and saving 101,309 lives.

7.
Ann Tour Res ; 88: 103182, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-36540368

RESUMO

In a context in which the tourism industry is jeopardised by the COVID-19 pandemic, and potentially by other pandemics in the future, the capacity to produce accurate forecasts is crucial to stakeholders and policy-makers. This paper attempts to forecast the recovery of tourism demand for 2021 in 20 destinations worldwide. An original scenario-based judgemental forecast based on the definition of a Covid-19 Risk Exposure index is proposed to overcome the limitations of traditional forecasting methods. Three scenarios are proposed, and ex ante forecasts are generated for each destination using a baseline forecast, the developed index and a judgemental approach. The limitations and potential developments of this new forecasting model are then discussed.

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