The complete mitochondrial genome of Zelkova schneideriana Hand-Mazz (Ulmaceae) and phylogenetic analysis.

Zelkova schneideriana Hand-Mazz is a second-class key protected wild plant in China. Here, the complete mitochondrial genome of Zelkova schneideriana Hand-Mazz was sequenced using Nanopore Sequel and Illumina NovaSeq platform. The mitochondrial genome was assembled into three circular-mapping molecules with the genome sizes of 154,640 bp, 192,388 bp and 146,907 bp, including 36 protein-coding genes, 19 tRNA genes, and 3 rRNA genes. Phylogenetic analysis indicated that Zelkova schneideriana Hand-Mazz is close with Hemiptelea davidii, a species in same Ulmaceae Mirb.

The complete mitochondrial genome sequence of Fragaria orientalis (Rosaceae).

Fragaria orientalis Lozinsk. is valuable germplasm material for cross breeding in Fragaria. In this study, we assembled the complete mitochondrial genome of F. orientalis using a combination of Illumina data and Nanopore data. The mitochondrial genome was 275,143 bp in length, including 29 protein-coding genes, 20 tRNA genes, and three rRNA genes, with a total GC content 45.23%. Seven protein-coding genes contained introns, and three were trans-spliced. Phylogenetic analysis indicated that F. orientalis is making a sister clade to the Amygdaloideae species. The complete mitochondrial genome of F. orientalis reported in this study will improve our understanding of Fragaria evolution.

Complete mitochondrial genome of Hemiptelea davidii (Ulmaceae) and phylogenetic analysis.

Hemiptelea davidii (Hance) Planch is a potential valuable forest tree in arid sandy environments. Here, the complete mitochondrial genome of H. davidii was assembled using a combination of the PacBio Sequel data and the Illumina Hiseq data. The mitochondrial genome is 460,941 bp in length, including 37 protein-coding genes, 19 tRNA genes, and three rRNA genes. The GC content of the whole mitochondrial genome is 44.84%. Phylogenetic analyses indicated that H. davidii is close with Cannabis and Morus species.

[Oxidation of cationic red 3R in water with H2O2 catalyzed by mineral loaded with Fe/Co].

Catalyst supported on pyrite was prepared by the impregnation method to enhance the activity of catalyst and characterized by scanning electron microscope (SEM), energy dispersive spectrometer (EDS) and X-ray diffraction (XRD). Some Fe2O3 and CoFe2O4 species were formed on the surface of pyrite. Synthetized catalyst was used to catalyze H2O2 oxidative discoloration of azo dye. Under the conditions of the catalyst adding dosage of 3 g x L (-1), H2O2 adding dosageof 0.3 mL x L(-1), and the oxidation reaction timeof 1 h, results showed that about 99.8% of color removal rate and 58.4% of TOC removal rate could be achieved. The active ingredients of catalyst were Fe2O3 and CoFe2O4. Hydroxyl radical was determined during the reaction by ESR technology, the chromophore of dyes could be destroyed with 1 min, and small molecular substances might be produced during the process according to the spectrum analysis. Reaction with wide pH ranges is beneficial to overcome the limitation of traditional Fenton reactions. This technology might be used as a potential alternative for treatment of recalcitrant wastewater.

Development and characterization of microsatellite markers in Prunus sibirica (Rosaceae).

PREMISE OF THE STUDY: Microsatellite loci were developed for Prunus sibirica to investigate genetic diversity, population genetic structure, and marker-assisted selection of late-blooming cultivars in the breeding of P. sibirica. • METHODS AND RESULTS: Using a magnetic bead enrichment strategy, 19 primer pairs were developed and characterized across 40 individuals from three P. sibirica wild populations and six individuals of P. armeniaca. The number of alleles per locus varied from three to 11 and the observed and expected heterozygosities ranged from 0.063 to 0.917 and 0.295 to 0.876, respectively, in the three P. sibirica wild populations. All primer pairs could be successfully amplified in six individuals of P. armeniaca. • CONCLUSIONS: These microsatellite primer pairs should be useful for population genetics, germplasm identification, and marker-assisted selection in the breeding of P. sibirica and related species.

Management of traumatic hemothorax by closed thoracic drainage using a central venous catheter.

OBJECTIVE: To evaluate the efficacy and safety of the treatment of traumatic hemothorax by closed pleural drainage using a central venous catheter (CVC), compared with using a conventional chest tube. METHODS: A prospective controlled study with the Ethics Committee approval was undertaken. A total of 407 patients with traumatic hemothorax were involved and they were randomly assigned to undergo closed pleural drainage with CVCs (n=214) or conventional chest tubes (n=193). The Seldinger technique was used for drainage by CVC, and the conventional technique for drainage by chest tube. If the residual volume of the hemothorax was less than 200 ml after the daily volume of drainage decreased to below 100 ml for two consecutive days, the treatment was considered successful. The correlative data of efficacy and safety between the two groups were analyzed using t or chi-squared tests with SPSS 13.0. A P value of less than 0.05 was taken as indicating statistical significance. RESULTS: Compared with the chest tube group, the operation time, fraction of analgesic treatment, time of surgical wound healing, and infection rate of surgical wounds were significantly decreased (P<0.05) in the CVC group. There were no significant differences between the two groups in the success rate of treatment and the incidence of serious complications (P>0.05), or in the mean catheter/tube indwelling time and mean medical costs of patients treated successfully (P>0.05). CONCLUSIONS: Management of medium or large traumatic hemothoraxes by closed thoracic drainage using CVC is minimally invasive and as effective as using a conventional large-bore chest tube. Its complications can be prevented and it has the potential to replace the large-bore chest tube.