Lead exposure of mainland Australia's top avian predator.

Lead (Pb) toxicity, through ingestion of lead ammunition in carcasses, is a threat to scavenging birds worldwide, but has received little attention in Australia. We analyzed lead exposure in the wedge-tailed eagle (Aquila audax), the largest raptor species found in mainland Australia and a facultative scavenger. Eagle carcasses were collected opportunistically throughout south-eastern mainland Australia between 1996 and 2022. Lead concentrations were measured in bone samples from 62 animals via portable X-ray fluorescence (XRF). Lead was detected (concentration >1 ppm) in 84% (n = 52) of the bone samples. The mean lead concentration of birds in which lead was detected was 9.10 ppm (±SE 1.66). Bone lead concentrations were elevated (10-20 ppm) in 12.9% of samples, and severe (>20 ppm) in 4.8% of samples. These proportions are moderately higher than equivalent data for the same species from the island of Tasmania, and are comparable to data from threatened eagle species from other continents. Lead exposure at these levels is likely to have negative impacts on wedge-tailed eagles at the level of the individual and perhaps at a population level. Our results suggest that studies of lead exposure in other Australian avian scavenger species are warranted.

Habitat selection by vulnerable golden bandicoots in the arid zone.

In 2010, vulnerable golden bandicoots (Isoodon auratus) were translocated from Barrow Island, Western Australia, to a mainland predator-free enclosure on the Matuwa Indigenous Protected Area. Golden bandicoots were once widespread throughout a variety of arid and semiarid habitats of central and northern Australia. Like many small-to-medium-sized marsupials, the species has severely declined since colonization and has been reduced to only four remnant natural populations. Between 2010 and 2020, the reintroduced population of golden bandicoots on Matuwa was monitored via capture-mark-recapture data collection, which was used in spatially explicit capture-recapture analysis to monitor their abundance over time. In 2014, we used VHF transmitters to examine the home range and habitat selection of 20 golden bandicoots in the enclosure over a six-week period. We used compositional analysis to compare the use of four habitat types. Golden bandicoot abundance in the enclosure slowly increased between 2010 and 2014 and has since plateaued at approximately one quarter of the density observed in the founding population on Barrow Island. The population may have plateaued because some bandicoots escape through the fence. Golden bandicoots used habitats dominated by scattered shrubland with spinifex grass more than expected given the habitat's availability. Nocturnal foraging range was influenced by sex and trapping location, whereas diurnal refuge habitat, which was typically under a spinifex hummock with minimal overstory vegetation, was consistent across sex and trapping location. Our work suggests that diurnal refuge habitat may be an important factor for the success of proposed translocations of golden bandicoots.

Mus musculus populations in Western Australia lack VKORC1 mutations conferring resistance to first generation anticoagulant rodenticides: Implications for conservation and biosecurity.

BACKGROUND: Humans routinely attempt to manage pest rodent populations with anticoagulant rodenticides (ARs). We require information on resistance to ARs within rodent populations to have effective eradication programs that minimise exposure in non-target species. Mutations to the VKORC1 gene have been shown to confer resistance in rodents with high proportions of resistance in mice found in all European populations tested. We screened mutations in Mus musculus within Western Australia, by sampling populations from the capital city (Perth) and a remote island (Browse Island). These are the first Australian mouse populations screened for resistance using this method. Additionally, the mitochondrial D-loop of house mice was sequenced to explore population genetic structure, identify the origin of Western Australian mice, and to elucidate whether resistance was linked to certain haplotypes. RESULTS: No resistance-related VKORC1 mutations were detected in either house mouse population. A genetic introgression in the intronic sequence of the VKORC1 gene of Browse Island house mouse was detected which is thought to have originated through hybridisation with the Algerian mouse (Mus spretus). Analysis of the mitochondrial D-loop reported two haplotypes in the house mouse population of Perth, and two haplotypes in the population of Browse Island. CONCLUSIONS: Both house mouse populations exhibited no genetic resistance to ARs, in spite of free use of ARs in Western Australia. Therefore weaker anticoagulant rodenticides can be employed in pest control and eradication attempts, which will result in reduced negative impacts on non-target species. Biosecurity measures must be in place to avoid introduction of resistant house mice, and new house mouse subspecies to Western Australia.

Completing a worldwide picture: preliminary evidence of lead exposure in a scavenging bird from mainland Australia.

Lead toxicity from ammunition has been shown to be a threat to scavenging birds across the globe. Despite decades of research in Europe, North and South America, Asia and Africa, there have been no studies to investigate this phenomenon in Australia despite that continent having many species of scavenging birds and widespread shooting practices. We present preliminary evidence of lead exposure in Australia's largest bird of prey, the wedge-tailed eagles (Aquila audax) through analysis of bone and eggshell samples from south-western Australia. From 11 bone samples, three birds (27%) had lead levels exceeding literature thresholds for elevated levels (>6.75 ppm). From 36 eggshell samples, no samples had lead levels >0.5 ppm, suggesting some limitations for this matrix as an indicator of lead exposure. Isotope ratios suggested ammunition as a likely source of the lead found in bone samples with elevated lead levels but other potential sources of lead require further investigation. Our preliminary results demonstrate that lead exposure is occurring in scavenging birds in Australia, and like the rest of the world, is likely to be derived from ammunition. This study supports an urgent call for further research into this worldwide phenomenon in Australia.

Anticoagulant rodenticide exposure in an Australian predatory bird increases with proximity to developed habitat.

Anticoagulant rodenticides (ARs) are commonly used worldwide to control commensal rodents. Second generation anticoagulant rodenticides (SGARs) are highly persistent and have the potential to cause secondary poisoning in wildlife. To date no comprehensive assessment has been conducted on AR residues in Australian wildlife. My aim was to measure AR exposure in a common widespread owl species, the Southern Boobook (Ninox boobook) using boobooks found dead or moribund in order to assess the spatial distribution of this potential threat. A high percentage of boobooks were exposed (72.6%) and many showed potentially dangerous levels of AR residue (>0.1 mg/kg) in liver tissue (50.7%). Multiple rodenticides were detected in the livers of 38.4% of boobooks tested. Total liver concentration of ARs correlated positively with the proportions of developed areas around points where dead boobooks were recovered and negatively with proportions of agricultural and native land covers. Total AR concentration in livers correlated more closely with land use type at the spatial scale of a boobook's home range than at smaller or larger spatial scales. Two rodenticides not used by the public (difethialone and flocoumafen) were detected in boobooks indicating that professional use of ARs contributed to secondary exposure. Multiple ARs were also detected in recent fledglings, indicating probable exposure prior to fledging. Taken together, these results suggest that AR exposure poses a serious threat to native predators in Australia, particularly in species using urban and peri-urban areas and species with large home ranges.

Anticoagulant rodenticide use, non-target impacts and regulation: A case study from Australia.

The impacts of anticoagulant rodenticides (ARs) on non-target wildlife have been well documented in Europe and North America. While these studies are informative, patterns of non-target poisoning of wildlife elsewhere in the world may differ substantially from patterns occurring in Australia and other countries outside of cool temperate regions due to differences in the types of ARs used, patterns of use, legislation governing sales, and potential pathways of secondary exposure. Most of these differences suggest that the extent and severity of AR poisoning in wildlife may be greater in Australia than elsewhere in the world. While many anecdotal accounts of rodenticide toxicity were found - especially in conjunction with government control efforts and island eradications - no published studies have directly tested rodenticide exposure in non-target Australian wildlife in a comprehensive manner. The effects of private and agricultural use of rodenticides on wildlife have not been adequately assessed. Synthesis of reviewed literature suggests that anticoagulant rodenticides may pose previously unrecognised threats to wildlife and indigenous people in Australia and other nations with diverse and abundant reptile faunas relative to countries with cooler climates where most rodenticide ecotoxicology studies have been conducted. To address the identified knowledge gaps we suggest additional research into the role of reptiles as potential AR vectors, potential novel routes of human exposure, and comprehensive monitoring of rodenticide exposure in Australian wildlife, especially threatened and endangered omnivores and carnivores. Additionally, we recommend regulatory action to harmonise Australian management of ARs with existing and developing global norms.