Biodiversity mitigates drought effects in the decomposer system across biomes.

Multiple facets of global change affect the earth system interactively, with complex consequences for ecosystem functioning and stability. Simultaneous climate and biodiversity change are of particular concern, because biodiversity may contribute to ecosystem resistance and resilience and may mitigate climate change impacts. Yet, the extent and generality of how climate and biodiversity change interact remain insufficiently understood, especially for the decomposition of organic matter, a major determinant of the biosphere-atmosphere carbon feedbacks. With an inter-biome field experiment using large rainfall exclusion facilities, we tested how drought, a common prediction of climate change models for many parts of the world, and biodiversity in the decomposer system drive decomposition in forest ecosystems interactively. Decomposing leaf litter lost less carbon (C) and especially nitrogen (N) in five different forest biomes following partial rainfall exclusion compared to conditions without rainfall exclusion. An increasing complexity of the decomposer community alleviated drought effects, with full compensation when large-bodied invertebrates were present. Leaf litter mixing increased diversity effects, with increasing litter species richness, which contributed to counteracting drought effects on C and N loss, although to a much smaller degree than decomposer community complexity. Our results show at a relevant spatial scale covering distinct climate zones that both, the diversity of decomposer communities and plant litter in forest floors have a strong potential to mitigate drought effects on C and N dynamics during decomposition. Preserving biodiversity at multiple trophic levels contributes to ecosystem resistance and appears critical to maintain ecosystem processes under ongoing climate change.

Identification of Bamboo Species Based on Extreme Gradient Boosting (XGBoost) Using Zhuhai-1 Orbita Hyperspectral Remote Sensing Imagery.

Mapping the distribution of bamboo species is vital for the sustainable management of bamboo and for assessing its ecological and socioeconomic value. However, the spectral similarity between bamboo species makes this work extremely challenging through remote sensing technology. Existing related studies rarely integrate multiple feature variables and consider how to quantify the main factors affecting classification. Therefore, feature variables, such as spectra, topography, texture, and vegetation indices, were used to construct the XGBoost model to identify bamboo species using the Zhuhai-1 Orbita hyperspectral (OHS) imagery in the Southern Sichuan Bamboo Sea and its surrounding areas in Sichuan Province, China. The random forest and Spearman's rank correlation analysis were used to sort the main variables that affect classification accuracy and minimize the effects of multicollinearity among variables. The main findings were: (1) The XGBoost model achieved accurate and reliable classification results. The XGBoost model had a higher overall accuracy (80.6%), kappa coefficient (0.708), and mean F1-score (0.805) than the spectral angle mapper (SAM) method; (2) The optimal feature variables that were important and uncorrelated for classification accuracy included the blue band (B1, 464-468 nm), near-infrared band (B27, 861-871 nm), green band (B5, 534-539 nm), elevation, texture feature mean, green band (B4, 517-523 nm), and red edge band (B17, 711-720 nm); and (3) the XGBoost model based on the optimal feature variable selection showed good adaptability to land classification and had better classification performance. Moreover, the mean F1-score indicated that the model could well balance the user's and producer's accuracy. Additionally, our study demonstrated that OHS imagery has great potential for land cover classification and that combining multiple features to enhance classification is an approach worth exploring. Our study provides a methodological reference for the application of OHS images for plant species identification.

Nitrogen addition alleviates water loss of Moso bamboo (Phyllostachys edulis) under drought by affecting light-induced stomatal responses.

The combined climate-change-evoked drought and nitrogen (N) deposition have severely affected plant carbon and water relations governed by stomata. However, the interplay between steady-state and dynamic stomatal behavior responses to light remains unclear regarding its impact on plant water and carbon relations. The objective here was to investigate whether light-induced stomatal dynamics could mitigate the adverse effects of steady-state gas exchange on water conservation or photosynthesis under drought and N addition conditions. We conducted a manipulative experiment to investigate the impacts of throughfall reduction, N addition, and their combination on light-induced stomatal and photosynthetic dynamics in a Moso bamboo (Phyllostachys edulis) forest. We determined the influence of stomal response rate on water loss and photosynthesis, and further assessed whether it mitigated the effects of steady-state gas exchange (gs). We found that Moso bamboo decreased gs under throughfall reduction, while accelerated stomatal opening and biochemical activation when irradiance increased, which reduced the lag in photosynthesis during the induction period. In contrast, under the combined throughfall reduction and N addition condition, Moso bamboo increased gs but showed faster stomatal closure, which decreased the percentage of transpiration following a decrease in light intensity. Our findings indicate that stomatal dynamic behavior may depend on the effects of steady-state gas exchange on water conservation and carbon uptake under different soil water and N conditions. These discoveries contribute to our understanding of the coupling mechanisms of plant water use and carbon uptake in the context of global changes.

Structural and electrochemical corrosion studies of spin coated ZrO2 thin films over stainless steel alloy for bone defect applications.

Sol-Gel-based reaction mixture sols have been long used to fabricate dense and uniform bioactive coatings with superior mechanical stability over metallic implants. On account of precise control over synthesis, fabrication, formed and low temperature of processing, this technology is one of the most feasible routes to produce bio-ceramic coatings. The study aims to develop a physical barrier over metal implants in form of bioinert Zirconia coatings, phase-stabilized using Dysprosium. The metallic substrates were cut into 10 mm × 10 mm samples and diamond polished after being polished with a 1000 grade emery sheet. Novel spin-coated zirconia films were fabricated over 316L Stainless steel substrates and were sintered at 600°C to obtain firm and uniform crack-free coatings. The thickness of the coatings was determined by ELCA-D meter thermal analysis was performed using TGA-DTA. Phase determination was performed using X-Ray diffraction followed by morphological investigations using Scanning electron microscopy. The corrosion resistance was evaluated with Polarization studies and electrokinetic data was derived using Tafel extrapolation. Biocompatibility evaluation was performed against MG-63 cell lines and RBCs along with bone-forming ability in vitro in SBF. Stable crack-free 3 Layer coatings fabricated at 2000 rpm for 3 s with a thickness of around 1 µm were found to be optimal for corrosion resistance behavior of steel implants at a low ICorr value of 0.501 µA/cm2 and adhesion strength of 40.93 MPa when untreated falling down to 39.92 MPa when immersed in SBF. The study concludes that medium rpm coatings sustain enough sol to produce crack-free coatings that form a strong physical barrier between body fluid and implant surface thereby reducing the attack of corrosive ions and protecting the implant surface without participating in any form of bioactivity but supporting native bone regeneration capabilities.

Investigation of the climatological impacts of agricultural management and abandonment on a boreal bog in western Newfoundland, Canada.

We compared greenhouse gas (GHG) fluxes and albedo of a pristine boreal bog and an adjacent abandoned peatland pasture in western Newfoundland, Canada to estimate the magnitude of radiative forcing (RF) created by agricultural drainage and abandonment. Our results indicated that these anthropogenic activities induced a climate cooling effect (negative RF), with the magnitude of the RF caused by the albedo change comparable to that induced by altered GHGs. Although the albedo-induced RF was positive in winter and negative in summer, the summer effect dominated because of greater solar radiation received. The climate cooling effect of GHGs change was due to an increase in the carbon dioxide sink capacity and a reduction in methane emissions under lower water table levels following agricultural drainage and abandonment. Calculation of sustained-flux global warming/cooling potentials also supported this finding. Our results show that the overall increase in albedo resulting from agricultural drainage and abandonment contributes significantly to the negative RF, strengthening the cooling effect due to the changing GHG fluxes. Therefore, changes in albedo due to altered vegetation coverage and hydrology and GHG fluxes should be considered when assessing the climatic impacts from land-use change in northern peatland.

Tradeoff between storage capacity and embolism resistance in the xylem of temperate broadleaf tree species.

Xylem traits are critical plant functional traits associated with water transport, mechanical support, and carbohydrate and water storage. Studies on the xylem hydraulic efficiency-safety tradeoff are numerous; however, the storage function of xylem parenchyma is rarely considered. The effects of a substantial number of xylem traits on water transport, embolism resistance, mechanical support, storage capacity and nonstructural carbohydrate (NSC) content were investigated in 19 temperate broadleaf species planted in an arid limestone habitat in northern China. There was no xylem hydraulic efficiency-safety tradeoff in the 19 broadleaf species. The total parenchyma fraction was negatively correlated with the fiber fraction. Embolism resistance was positively correlated with indicators of xylem mechanical strength such as vessel wall reinforcement, vessel wall thickness and fiber wall thickness, and was negatively related to the axial parenchyma fraction, especially the paratracheal parenchyma fraction. The paratracheal parenchyma fraction was positively correlated with the ratio of the paratracheal parenchyma fraction to the vessel fraction. In addition, the xylem NSC concentration was positively related to the total parenchyma fraction and axial parenchyma fraction. There was a storage capacity-embolism resistance tradeoff in the xylem of 19 broadleaf species in arid limestone habitats. We speculate that the temperate broadleaf species may show a spectrum of xylem hydraulic strategies, from the embolism resistance strategy related to a more negative P50 (the water potential corresponding to 50% loss of xylem conductivity) to the embolization repair strategy based on more paratracheal parenchyma.

Microbe-mediated attenuation of soil respiration in response to soil warming in a temperate oak forest.

Soil respiration (Rs) in response to climate warming received a wide concern due to its important role in terrestrial ecosystem carbon (C) cycling, but the warming-induced effects of soil microbes on soil respiration are still less understood, especially over time. Our study aims to understand the long-term warming induced effects of soil microbes on Rs. A field soil warming experiment using a completely randomized design was conducted in a naturally regenerated oak forest (Quercus aliena) in central China. Soil warming was executed by infrared heater throughout the period from 2011 to 2015. Our results showed that soil temperature was a main factor in regulating Rs in a temperate oak forest throughout the experiment, while soil water content determined Rs only when a naturally dry year occurred. The positive effect of soil warming on Rs that was observed (i.e., 37.5 to 42.0% in the first two years) gradually diminished in the following three years (i.e., 0.9 to 15.4%). Significant positive warming effects on the temperature sensitivity of Rs (Q10) only occurred in the second year. Continuous soil warming caused the decline in nitrogen (N) availability, with a significant increase in microbial biomass-specific enzyme activities for N-acquisition. The attenuation of microbial biomass increment and the decreased ratio of enzymatic C:N acquisition contributed to the diminished warming effect on Rs over time. Our study suggests that microbe-mediated attenuation of Rs, accompanied by the concomitant decline in soil N availability in response to warming, should be taken into consideration in global C cycle modeling.

Soil nitrogen determines greenhouse gas emissions from northern peatlands under concurrent warming and vegetation shifting.

Boreal peatlands store an enormous pool of soil carbon that is dependent upon - and vulnerable to changes in - climate, as well as plant community composition. However, how nutrient availability affects the effects of climate and vegetation change on ecosystem processes in these nutrient-poor ecosystems remains unclear. Here we show that although warming promoted higher CH4 emissions, the concurrent addition of N counteracted most (79%) of this effect. The regulation effects of the vegetation functional group, associated with the substrate quality, suggest that CH4 emissions from peatlands under future warming will be less than expected with predicted shrub expansion. In contrast, N2O flux will be enhanced under future warming with predicted shrub expansion. Our study suggests that changes in greenhouse gas emissions in response to future warming and shifts in plant community composition depend on N availability, which reveals the complex interactions that occur when N is not a limiting nutrient.

Prediction of temperate broadleaf tree species mortality in arid limestone habitats with stomatal safety margins.

A growing body of evidence highlights the occurrence of increased widespread tree mortality during climate change-associated severe droughts; however, in situ long-term drought experiments with multispecies communities for the prediction of tree mortality and exploration of related mechanisms are rather limited in natural environments. We conducted a 7-year afforestation trial with 20 drought-resistant broadleaf tree species in an arid limestone habitat in northern China, where the species displayed a broad range of survival rates. The stomatal and xylem hydraulic traits of all the species were measured. We found that species' stomatal closure points were strongly related to their xylem embolism resistance and xylem minimum water potential but not to their survival rates. Hydraulic failure of the vascular system appeared to be the main cause of tree mortality, and the stomatal safety margin was a better predictor of tree mortality than the traditionally considered xylem embolism resistance and hydraulic safety margin. We recommend the stomatal safety margin as the indicator for predicting drought-induced tree mortality and for selecting tree species in future forest restorations in arid regions.

Tree species diversity promotes soil carbon stability by depressing the temperature sensitivity of soil respiration in temperate forests.

The diversity-stability interrelationship suggests that high diversity can buffer fluctuations in environmental conditions such as temperature; we thus hypothesize that tree species diversity will lower the temperature sensitivity of soil respiration (Rs), known as Q10 value. Our hypothesis was tested in a deciduous broad-leaf and a coniferous-broad-leaf mixedwood stand in the warm temperate region in China. We measured soil respiration and indices of tree species diversity including species richness (S), the Berger-Parker index (d), the Simpson index (λ), the Shannon index (He'), and the Pielou evenness index (Je). Our results generally confirm our hypothesis that Q10 was positively correlated to λ, but negatively related to He', d, and Je, and independent of S, in both stands. However, Rs was independent of the diversity indices. These findings imply that tree species diversity promotes soil carbon stability by depressing the Q10. Furthermore, different biotic and abiotic variables explained the variations of species diversity and Q10 in the broad-leaf and mixedwood forests, suggesting that the mechanisms underlining the effects of tree species diversity on Q10 are different between the two forest types. We conclude that sustainable forest management that improves tree species diversity will increase soil carbon stability and benefit our efforts to mitigate climate change.

Near-zero methane emission from an abandoned boreal peatland pasture based on eddy covariance measurements.

Although estimates of the annual methane (CH4) flux from agriculturally managed peatlands exist, knowledge of controls over the variation of CH4 at different time-scales is limited due to the lack of high temporal-resolution data. Here we present CH4 fluxes measured from May 2014 to April 2016 using the eddy covariance technique at an abandoned peatland pasture in western Newfoundland, Canada. The goals of the study were to identify the controls on the seasonal variations in CH4 flux and to quantify the annual CH4 flux. The seasonal variation in daily CH4 flux was not strong in the two study years, however a few periods of pronounced emissions occurred in the late growing season. The daily average CH4 flux was small relative to other studies, ranging from -4.1 to 9.9 nmol m-2 s-1 in 2014-15 and from -7.1 to 12.1 nmol m-2 s-1 in 2015-16. Stepwise multiple regression was used to investigate controls on CH4 flux and this analysis found shifting controls on CH4 flux at different periods of the growing season. During the early growing season CH4 flux was closely related to carbon dioxide fixation rates, suggesting substrate availability was the main control. The peak growing season CH4 flux was principally controlled by the CH4 oxidation in 2014, where the CH4 flux decreased and increased with soil temperature at 50 cm and soil water content at 10 cm, but a contrasting temperature-CH4 relation was found in 2015. The late growing season CH4 flux was found to be regulated by the variation in water table level and air temperature in 2014. The annual CH4 emission was near zero in both study years (0.36 ± 0.30 g CH4 m-2 yr-1 in 2014-15 and 0.13 ± 0.38 g CH4 m-2 yr-1 in 2015-16), but fell within the range of CH4 emissions reported for agriculturally managed peatlands elsewhere.

Experimental throughfall reduction barely affects soil carbon dynamics in a warm-temperate oak forest, central China.

Changing precipitation patterns could affect soil carbon (C) cycling in China's forests. A throughfall reduction (TFR) experiment was conducted in a warm-temperate oak forest in central China to examine effects of reduced precipitation on total soil respiration (SR), heterotrophic soil respiration (HR), autotrophic soil respiration (AR), soil microbial biomass, and fine root biomass from 2013 to 2016. Rain-out shelters, excluding ~50% of throughfall, were applied between May and September, thereby simulating a ~30% reduction in annual precipitation. Although soil moisture was significantly reduced during TFR, microbial biomass and HR remained unaffected. SR, AR, as well as fine root biomass increased during TFR in a comparable dry year, but remained unaffected during all other years. Annual rates of SR, HR, and AR were all unaffected by TFR. Our results indicate that a mild, steady, reduction in growing season precipitation does not affect soil organic matter decomposition in the oak forest ecosystem studied. Low SR rates during a natural dry-spell indicate that SR can be significantly decreased under more severe drought than imposed by the TFR treatment. Our data suggest a low soil moisture threshold of about 10 vol% for SR in the studied soil.

Tamoxifen reduces P-gp-mediated multidrug resistance via inhibiting the PI3K/Akt signaling pathway in ER-negative human gastric cancer cells.

Multidrug resistance (MDR), mediated by overexpression of drug efflux transporters such as P-glycoprotein (P-gp), is a major problem limiting successful chemotherapy of gastric cancer. Tamoxifen (TAM), a triphenylethylene nonsteroidal antiestrogen agent, shows broad-spectrum antitumor properties. Emerging studies demonstrated that TAM could significantly reduce the MDR in a variety of human cancers. Here we investigated the effects and possible underlying mechanisms of action of TAM on the reversion of MDR in ER-negative human gastric cancer cells. Our results demonstrated that in MDR phenotype SGC7901/CDDP gastric cancer cells TAM dramatically lowered the IC50 of CDDP, 5-FU and ADM, increased the intracellular Rhodamine123 accumulation and induced G0/G1 phase arrest, while G2/M phase decreased accordingly. Furthermore, at the molecular level, TAM substantially decreased the expression of P-gp, p-Akt and the Akt-regulated downstream effectors such as p-GSK-3ß, p-BAD, Bcl-XL and cyclinD1 proteins without affecting the expression of t-Akt, t-GSK-3ß, t-BAD proteins in SGC7901/CDDP cells. Thus, our findings demonstrate that TAM reverses P-gp-mediated gastric cancer cell MDR via inhibiting the PI3K/Akt signaling pathway.

Factors affecting spatial variation of annual apparent Q10 of soil respiration in two warm temperate forests.

A range of factors has been identified that affect the temperature sensitivity (Q10 values) of the soil-to-atmosphere CO2 flux. However, the factors influencing the spatial distribution of Q10 values within warm temperate forests are poorly understood. In this study, we examined the spatial variation of Q10 values and its controlling factors in both a naturally regenerated oak forest (OF) and a pine plantation (PP). Q10 values were determined based on monthly soil respiration (R(S)) measurements at 35 subplots for each stand from Oct. 2008 to Oct. 2009. Large spatial variation of Q10 values was found in both OF and PP, with their respective ranges from 1.7 to 5.12 and from 2.3 to 6.21. In PP, fine root biomass (FR) (R = 0.50, P = 0.002), non-capillary porosity (NCP) (R = 0.37, P = 0.03), and the coefficients of variation of soil temperature at 5 cm depth (CV of T5) (R = -0.43, P = 0.01) well explained the spatial variance of Q10. In OF, carbon pool lability reflected by light fractionation method (LLFOC ) well explained the spatial variance of Q10 (R = -0.35, P = 0.04). Regardless of forest type, LLFOC and FR correlation with the Q10 values were significant and marginally significant, respectively; suggesting a positive relationship between substrate availability and apparent Q10 values. Parameters related to gas diffusion, such as average soil water content (SWC) and NCP, negatively or positively explained the spatial variance of Q10 values. Additionally, we observed significantly higher apparent Q10 values in PP compared to OF, which might be partly attributed to the difference in soil moisture condition and diffusion ability, rather than different substrate availabilities between forests. Our results suggested that both soil chemical and physical characters contributed to the observed large Q10 value variation.

[Research advances in forest soil respiration].

Among the methods of measuring forest soil respiration, infrared CO2 analysis is the optimal one so far. Comparing with empirical model, the process-based model in simulating the production and transportation of soil CO2 has the advantage of considering the biological and physical processes of soil respiration. Generally, soil respiration is positively correlated with soil temperature and moisture, but there are still many uncertainties about the relationships between soil respiration and forest management activities such as firing, cutting, and fertilization. The relationships of soil respiration with vegetation type and soil microbial biomass, as well as the spatial heterogeneity of soil respiration, are the hotspots in recent researches. Some issues and future development in forest soil respiration research were discussed in this paper.