Anti-Herpes Simplex Virus Type 1 Activity Evaluation of Natural Derived Phloroglucinol Derivatives and Their Molecular Mechanisms Study.

HSV-1 is a common infection that can cause cold sores. In this study, the anti-HSV-1 virus activity of three series compounds A1-A9, B1-B12, C1-C22 was screened by MTT assay, qRT-PCR assay, Western blot assay and viruses' plaque assays. The results of MTT assay disclosed that phloroglucinol derivatives C2 and C3 effectively inhibited the death of HSV-1 infected vero cells with the CC50 values of C2 and C3 were 72.64 µmol/L and 32.62 µmol/L in HaCaT cells, 137.6 µmol/L and 48.55 µmol/L in Hela cells. The IC50 values of C3 in vero cells and Hela cells were 19.26 µmol/L and 22.98 µmol/L, respectively. In the qRT-PCR experiments, it showed that C2 and C3 effectively reduced the synthesis of HSV-1 early viral gene VP16 and late viral gene gD. The Western blot results showed that both C2 and C3 inhibited the expression of HSV-1 gD protein in a concentration-dependent manner. Lastly, viruses' plaque assay results showed that C2 and C3 inhibited the production of HSV-1 progeny virus in Hela cells and HaCaT cells in a concentration-dependent manner. Taken together, these results suggest that C2 and C3 are promising candidate that warrants further attention in the development of anti-HSV-1 drugs.

Identifification of a novel LDLR-RET Fusion in Lung Adenocarcinoma.

BACKGROUND: REarranged during Transfection (RET) gene fusion is one of the common oncogenic variants detectable in non-small cell lung cancer (NSCLC). The feature of most oncogenic RET gene fusion cases is that RET tyrosine kinase domain is retained in fusions and the partner gene includes a coiled-coil or LIS1 homology domain. However, only a few studies reported about the exceptional form of RET fusion in NSCLC so far. METHODS: Targeted next-generation sequencing (NGS) and fluorescence in situ hybridization (FISH) were performed on resected cancerous tissue. RESULTS: A rare form of RET fusion was identified in a 45 year-old Chinese female patient, in which exon 1-4 of LDLR fused with exon 12-21 of RET. The result was validated by FISH. CONCLUSIONS: This novel form of RET fusion in NSCLC is reported for the first time worldwide, offering a new treatment option for the patient with the possibility of using RET-selective inhibitors.

Can 5-month-old infants consider the perspective of a novel eyeless agent? New evidence for early mentalistic reasoning.

Is early reasoning about an agent's knowledge best characterized by a mentalistic stance, a teleological stance, or both? In this research, 5-month-old infants (N = 64, 50% female, 83% White) saw a novel eyeless agent consistently approach object-A as opposed to object-B. Although infants could always see both objects, a screen separated object-B from the agent. When object-B protruded above the screen, infants interpreted the agent's actions as revealing a preference for object-A over object-B. When object-B did not protrude above the screen, however, infants refrained from attributing such a preference: Consistent with mentalistic accounts, they reasoned that the agent's representation of the scene did not include object-B, and they used the agent's incomplete representation, non-egocentrically, to interpret its actions.

Infants' selective imitation of a transitive agent and an intransitive agent.

This study examined how the reliability (i.e., transitivity) of an agent's object choices affects 16-month-old infants' (N = 48) imitation of her unconventional way of turning on a touch light box with her head when her hands were available. When the agent made transitive choices (i.e., she chose Object A over Object B, Object B over Object C, and then A over C), infants imitated her head touch actions. When the agent made intransitive choices (i.e., after choosing A over B and B over C, she chose C over A), infants were more likely to use only their hands to touch the light box. In addition, when it was presumably difficult for infants to judge the transitivity of the agent's choices (i.e., she chose B over C, A over B, and then A over C), they used their hands more. These results demonstrate that infants' understanding informs their decisions to selectively imitate others' specific ways to act on novel artifacts, consistent with young children's selective trust in information provided by other people based on their epistemic reliability.

How do 3-month-old infants attribute preferences to a human agent?

The current study showed that 3-month-old infants attributed a preference to a human agent, with her face and upper body visible, when she consistently reached for and grasped one of two objects with her bare hand. In contrast, infants did not appear to interpret the agent's same actions of grasping the object as indicative of her preference when it was the only object present or when it hid the other object from her but not from the infants. These results suggest that even from an early age, infants interpret human agents' actions in terms of mental states such as goals and preferences. In light of the current results, mechanisms for early psychological understanding are discussed.

Is it a Container? Young Infants' Understanding of Containment Events.

The present research examined how certain features of a box affected 4.5-month-old infants' interpretation of containment events the box was involved in. If the box was a regular container, infants did not respond with increased attention when a tall cylinder became fully hidden after being lowered inside the box, consistent with previous research. In contrast, if a three-sided object (the box without its back) replaced the box, or if shown that the box had a removable back, infants were able to detect the height violations, 3 months earlier than they normally would. These results demonstrate how infants' perception or representation of objects interplays with their interpretation of physical events these objects involve in.

Infants' Understanding of Preferences When Agents Make Inconsistent Choices.

This study showed that 8.5-month-old infants seemed to consider the consistency of an agent's choices in attributing preferences to her. When the agent consistently chose one object over another, three or four times consecutively, infants acted as if they had interpreted her actions as evidence for her preference. In contrast, when the agent inconsistently chose between the two objects, at the ratio of 1:3, infants did not seem to interpret her actions as suggesting her preference. Converging evidence was obtained from infants' responses across a looking-time task and an action task. The results are discussed in terms of how infants might use frequencies of agents' actions directed toward different objects to understand agents' preferences.

13-month-olds' understanding of social interactions.

In the present research, we investigated how 13-month-olds use their emergent theory-of-mind understanding (i.e., understanding about other people's mental states, such as their intentions, perceptions, and beliefs) and social-evaluation skills to make sense of social interactions. The infants watched three puppets (A, B, and C) interact. The results showed that after seeing Agents A and B interact in a positive manner, infants expected them to continue doing so, even after they saw B hit another agent, C, while A was absent. When A was present to witness B's harmful action, however, infants expected A to change his or her behavior and ignore B. Therefore, infants seemed to consider A's perspectives when predicting A's actions. Furthermore, if B accidentally hit C when A was present, infants seemed to accept that A could interact or not interact with B, which suggests that they had taken into account B's intention in their interpretations of the agents' interactions.

Three-dimensional study of poly(lactic co-glycolic acid) micro-porous microspheres using hard X-ray nano-tomography.

Poly(lactic co-glycolic acid) (PLGA) is widely used in diverse fields, especially in delivering biologically active proteins and drugs. For these applications, the knowledge of morphology and microstructure of PLGA micro-porous microspheres is of great importance since they strongly influence the drug delivering efficiency. In this study, micro-porous PLGA microspheres loaded by bovine serum albumin are investigated by using a full-field Zernike phase contrast transmission hard X-ray microscope. From three-dimensional reconstructions and segmentations, fundamental microstructural parameters such as size, shape, distribution and volume ratio among pores and proteins inside PLGA microspheres were obtained. These parameters are useful to understand the relationship between the internal microstructure and drug encapsulation, as well as the drug release efficiency of PLGA microspheres. The presented results demonstrate the capability of hard X-ray nano-tomography to characterize porous microspheres loaded with proteins and drugs, and also open a way to analyse, optimize and design new PLGA microspheres for specific applications.

Can infants make transitive inferences?

Researchers have long been interested in the emergence of transitive reasoning abilities (e.g., if A>B and B>C, then A>C). Preschool-aged children are found to make transitive inferences. Additionally, nonhuman animals demonstrate parallel abilities, pointing to evolutionary roots of transitive reasoning. The present research examines whether 16-month-old infants can make transitive inferences about other people's preferences. If an agent prefers object-A over B (A>B) and B over C (B>C), infants seem to reason that she also prefers A over C (A>C) (Experiment 1). Experiment 2 provides indirect evidence that a one-directional linear ordering of the three items (A>B>C) may have helped infants to succeed in the task. These and control results present the first piece of evidence that precursors of transitive reasoning cognitive abilities exist in infancy.

Understanding preferences in infancy.

A preference is defined as a dispositional state that helps explain why a person chooses one option over another. Preference understanding is a significant part of interpreting and predicting others' behavior, which can also help to guide social encounters, for instance, to initiate interactions and even form relationships based on shared preferences. Cognitive developmental research in the past several decades has revealed that infants have relatively sophisticated understandings about others' preferences, as part of investigations into how young children make sense of others' behavior in terms of mental states such as intentions, dispositions including preferences, and epistemic states. In recent years, research on early psychological knowledge expands to including infant understanding of social situations. As such, infants are also found to use their preference understandings in their social life. They treat favorably others who share their own preferences, and they prefer prosocial and similar others (e.g., those who speak their language). In reviewing these results, we point out future directions for research and conclude with further suggestions and recommendations. This article is categorized under: Cognitive Biology > Cognitive Development Psychology > Development and Aging.

Young infants' expectations about a self-propelled agent's body.

What intuitive biological understandings do infants have? Recent work reports that 8-month-olds seem to identify self-propelled agents as animals and expect them to have a closed body. The present study examined a group of 6.5-month-old infants' (N = 50, 52% female, 84% White) biological expectations. The infants seemed to grasp the causal link between a novel self-propelled box agent's functioning and its body because they expected a temporary operation (i.e., an experimenter opening the box, exposing its insides, and closing it) to impair its ability to move. Further, infants accepted what was shown inside the box during the operation; whether it had an internal cuboid did not affect the results. Together, this suggests that infants at this young age appear to recognize the importance of having an intact body to a novel self-propelled agent's mobility but have no specific knowledge about what should be inside such an entity. These findings thus shed new light on the developmental origins of biological understandings.

Distribution of polycyclic aromatic hydrocarbons in indoor/outdoor window films and the indoor film/air partition of northeastern Chinese college dormitories.

Indoor window films can represent short-term air pollution conditions of indoor environment through rapidly capturing organic contaminants as effective passive air samplers. To investigate the temporal variation, influence factors of polycyclic aromatic hydrocarbons (PAHs) in indoor window films, and the exchange behavior with gas phase in college dormitories, 42 pairs window films of interior and exterior window surfaces and corresponding indoor gas phase and dust samples were collected monthly in six selected dormitories, Harbin, China, from August to December 2019 and September 2020. The average concentration of ∑16PAHs in indoor window films (398 ng/m2) was significantly (p < 0.01) lower than that in outdoors (652 ng/m2). In addition, the median indoor/outdoor ∑16PAHs concentration ratio was close to 0.5, showing that outdoor air acted as a major PAH source to indoor environment. The 5-ring PAHs were mostly dominant in window films whereas the 3-ring PAHs contributed mostly in gas phase. 3-ring PAHs and 4-ring PAHs were both important contributors for dormitory dust. Window films showed stable temporal variation, i.e. PAH concentrations in heating months were higher than those in non-heating months. The atmospheric O3 concentration was the main influence factor of PAHs in indoor window films. PAHs with low molecular weight in indoor window films rapidly reached film/air equilibrium phase within in dozens of hours. The large deviation in the slope of the log KF-A versus log KOA regression line from that in reported equilibrium formula might be the difference between the window film composition and octanol.

Three-month-old infants attribute goals to a non-human agent.

The present research examined whether 3-month-old infants, the youngest found so far to engage in goal-related reasoning about human agents, would also act as if they attribute goals to a novel non-human agent, a self-propelled box. In two experiments, the infants seemed to have interpreted the box's actions as goal-directed after seeing the box approach object A as opposed to object B during familiarization. They thus acted as though they expected the box to maintain this goal and responded with increased attention when the box approached object B during test. In contrast, when object B was absent during familiarization and introduced afterwards, the infants' responses were consistent with their having recognized that they had no information to predict which of the two objects the box should choose during test and therefore responded similarly when the box approached either object. However, if object B was absent during familiarization and object A was in different positions but the box persistently approached it, thus demonstrating equifinal variations in its actions, the infants again acted as though they attributed to the box a goal directed towards object A and expected the box to maintain this goal even when object B was introduced and hence responded with prolonged looking when the box failed to do so during test. These results are consistent with the notion that (a) infants as young as 3 months appear to attribute goals to both human and non-human agents, and (b) even young infants can use certain behavioral cues, e.g. equifinal variations in agents' actions, to make inferences about agents' goals.

Do you see what I see? Infants' reasoning about others' incomplete perceptions.

Twelve-month-olds realize that when an agent cannot see an object, her incomplete perceptions still guide her goal-directed actions. What would happen if the agent had incomplete perceptions because she could see only one part of the object, for example one side of a screen? In the present research, 16-month-olds were first shown an agent who always pointed to a red object, as opposed to a black or a yellow object, suggesting that she preferred red over the other colours. Next, two screens were introduced while the agent was absent. The screens were (1) red or green on both sides; (2) red on the front (infants' side) but green on the back (the agent's side) or vice versa; or (3) only coloured red or green on the front. During test, the agent, who could see only the back of the screens, pointed to one of the two screens. The results revealed that while infants expected the agent to continue acting on her colour preference and point to the red rather than the green screen during test, they did so in accord with the agent's perception of the screens, rather than their own perceptions: they expected the agent to point to the red screen in (1), but to the green-front screen in (2), and they had no prediction of which screen the agent should point to in (3). The implications of the present findings for early psychological reasoning research are discussed.

Do 8-Month-Old Infants Consider Situational Constraints When Interpreting Others' Gaze as Goal-Directed Action?

Some actions of agents are ambiguous in terms of goal-directedness to young infants. If given reasons why an agent performed these ambiguous actions, would infants then be able to perceive the actions as goal-directed? Prior results show that infants younger than 12 months can not encode the relationship between a human agent's looking behavior and the target of her gaze as goal-directed. In the present experiments, 8-month-olds responded in ways suggesting that they interpreted an agent's action of looking at object-A as opposed to object-B as evidence for her goal directed toward object-A, if her looking action was rational given certain situational constraints: a barrier separated her from the objects or her hands were occupied. Therefore, the infants seem to consider situational constraints when attributing goals to agents' otherwise ambiguous actions; they seem to realize that within such constraints, these actions are efficient ways for agents to achieve goals.

Infant expectations of instant or delayed gratification.

Choices between immediate gratification and long-term (but larger) gains are prevalent in human life, which is why the decision-making processes to delay gratification have been studied extensively throughout different developmental ages. Children's delay-of-gratification behaviors have been examined in the well-known "marshmallow test," in which 3- to 5-year-olds are given a marshmallow and told by an experimenter that they can eat it immediately or wait for an unspecified duration of time (which can be capped at 15 min) until the experimenter returns so that they can receive another marshmallow. Children's wait time has been viewed as a good indicator of their later development. Here we show that a group of 22-month-old infants (N = 32) already held expectations about others' choices in a violation-of-expectation looking-time task modeled after the marshmallow test. The infants expected an agent to defer gratification based on a speaker's promise of the second marshmallow available in the future, but to eat the currently attainable marshmallow when the speaker made no such promise. Our findings indicate an early-emerging understanding of others' choices of delayed or instant gratification and shed new light on the development of delay-of-gratification behaviors.

Trust in early childhood.

Children need to build trust in their primary caregivers or significant others, as well as people who are unrelated to them, including those who belong to different social groups. The present chapter focuses on children's trust in unfamiliar individuals. How do they determine who to trust? Does putting trust in another person operate differently depending on the specific issue at hand? To address these questions, we differentiate between two forms of trust: children's trust in others' epistemic states to learn from others (epistemic trust) and trusting others for social support and reassurance (social trust), for example, when to expect that interaction partners will be truthful and keep promises. We first review the literature on epistemic trust to show that young children seem to value others' accuracy and competence when learning from them, even when these individuals are from different linguistic or racial groups than their own. We then present findings on social trust suggesting that young children trust those who are well-meaning and who keep their promises. Finally, we raise the question of whether there are environmental influences on the interaction of both epistemic and social trust with intergroup factors such as race, and we propose that research with infants will be useful to better illuminate the developmental roots of human trust.

Young infants' reasoning about physical events involving inert and self-propelled objects.

The present research examined whether 5- to 6.5-month-old infants would hold different expectations about various physical events involving a box after receiving evidence that it was either inert or self-propelled. Infants were surprised if the inert but not the self-propelled box: reversed direction spontaneously (Experiment 1); remained stationary when hit or pulled (Experiments 3 and 3A); remained stable when released in midair or with inadequate support from a platform (Experiment 4); or disappeared when briefly hidden by one of two adjacent screens (the second screen provided the self-propelled box with an alternative hiding place; Experiment 5). On the other hand, infants were surprised if the inert or the self-propelled box appeared to pass through an obstacle (Experiment 2) or disappeared when briefly hidden by a single screen (Experiment 5). The present results indicate that infants as young as 5 months of age distinguish between inert and self-propelled objects and hold different expectations for physical events involving these objects, even when incidental differences between the objects are controlled. These findings are consistent with the proposal by Gelman, R. (1990). First principles organize attention to and learning about relevant data: Number and the animate-inanimate distinction as examples. Cognitive Science, 14, 79-106, Leslie, A. M. (1994). ToMM, ToBY, and Agency: Core architecture and domain specificity. In L. A. Hirschfeld & S. A. Gelman (Eds.), Mapping the mind: Domain specificity in cognition and culture (pp. 119-148). New York: Cambridge University Press, and others that infants endow self-propelled objects with an internal source of energy. Possible links between infants' concepts of self-propelled object, agent, and animal are also discussed.

Recognizing the role of perception in action at 6 months.

The present research examined whether infants as young as 6 months of age would consider what objects a human agent could perceive when interpreting her actions on the objects. In two experiments, the infants took the agent's actions of repeatedly reaching for and grasping one of two possible objects as suggesting her preference for that object only when the agent could detect both objects, not when the agent's perceptual access to the second object was absent, either because a large screen hid the object from the agent (Experiment 1), or because the agent sat with her back toward the object (Experiment 2). These results suggest that young infants recognize the role of perception in constraining an agent's goal-actions.