RESUMO
A Gram-stain-negative, aerobic, motile with flagella and rod- or ovoid-shaped bacterium, designated GG15T, was isolated from tidal flat sediment sampled in Zhoushan, Zhejiang Province. Strain GG15T grew at 20-40â°C (optimum, 30â°C), at pH 5.5-9.5 (optimum, pH 7.0-8.0) and with 1.0-10.0â% (w/v) NaCl (optimum, 1.5â%). Colony diameters ranged from 1 to 3 mm within the first week, reaching a maximum of 6-7 mm after 15 days of cultivation. Strain GG15T exhibited highest 16S rRNA gene sequence similarity to Microbulbifer taiwanensis CCM 7856T (98.1 %), with similarity to other species within the genus Microbulbifer ranging from 97.8 to 93.8â%. Similarity values to other genera were below 93.8â%. Strain GG15T exhibited positive activity for ß-glucosidase, trypsin and chymotrypsin, whereas the reference strain showed negative activity. Chemotaxonomic analyses indicated that strain GG15T contained Q-8 as the sole respiratory quinone, C16â:â0 (9.1â%), iso-C15â:â0 (30.9â%) and iso-C11â:â0 3-OH (7.2â%) as the predominant fatty acids, and phosphatidylethanolamine, phosphatidylglycerol, three unidentified lipids, four unidentified glycolipids, one unidentified phospholipid, two unidentified aminolipids and two unidentified aminophospholipids as the main polar lipids. The genome of strain GG15T was 4â307â641 bp long, comprising 3861 protein-coding genes. The G+C content of strain GG15T was 61.5âmol% based on its genomic sequence. Strain GG15T showed low digital DNA-DNA hybridization (<70â%) and average nucleotide identity values (<95â%) with other Microbulbifer species. As a result, a novel species within the genus Microbulbifer, named Microbulbifer magnicolonia sp. nov., is proposed. The type strain is GG15T (MCCC 1K08802T=KCTC 8210T).