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1.
Aesthet Surg J ; 38(7): 763-769, 2018 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-29360916

RESUMO

BACKGROUND: Several studies have demonstrated that progressive tension sutures (PTS) reduce seroma. Many fear that adding liposuction to abdominoplasty will increase seroma rates and avoid drainless abdominoplasty when performing concomitant liposuction. OBJECTIVES: We sought to identify whether liposuction increases seroma in PTS and non-PTS abdominoplasty. METHODS: In this retrospective study, 619 patients underwent abdominoplasty between 2009 and 2017, of which 299 patients had drainless abdominoplasty with PTS and 320 had drain-based abdominoplasty. We compared complications among PTS patients with and without liposuction and among drain-based abdominoplasty patients with and without liposuction. RESULTS: Demographics were similar between PTS and drain patients and between liposuction and nonliposuction PTS. Mean liposuction volume with PTS was 1592 ± 1048 mL. Seroma in the PTS group was found to be 2.6%, which is consistent with previous data. PTS without liposuction had a rate of seroma of 6.67% compared to a rate of 2.2% with liposuction; these rates were not significantly different (P = 0.20). A total of 207 patients had drain-based abdominoplasty with liposuction, and 113 had it without liposuction. Seroma with liposuction was 9.17% and without liposuction was 6.19%, although these differences were not significant (P = 0.52). PTS lipoabdominoplasty had less seroma compared with drain-based lipoabdominoplasty (P = 0.01). CONCLUSIONS: Liposuction was performed in 80% of the patients, and patients with lipoabdominoplasty were not at a higher risk of seroma, in the drain group or the PTS group. More patients may allow validation that liposuction may actually be protective with PTS. Regardless, there is no increase in seroma with the addition of liposuction to PTS drainless abdominoplasty.


Assuntos
Contorno Corporal/métodos , Lipoabdominoplastia/métodos , Complicações Pós-Operatórias/epidemiologia , Seroma/epidemiologia , Técnicas de Sutura/efeitos adversos , Adulto , Idoso , Contorno Corporal/efeitos adversos , Feminino , Humanos , Lipoabdominoplastia/efeitos adversos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/etiologia , Estudos Retrospectivos , Seroma/etiologia , Resultado do Tratamento , Adulto Jovem
2.
Aesthet Surg J ; 38(8): 861-869, 2018 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-29365056

RESUMO

BACKGROUND: As the popularity of aesthetic gluteoplasty continues to grow, there is renewed focus on defining the ideal buttocks. However, the literature lacks studies characterizing an ideal thigh, despite the impact of thigh contour on overall gluteal aesthetic. OBJECTIVES: The authors performed the first population analysis of the characteristics of perception of attractive thighs, to identify a role for fat grafting of the thigh in gluteoplasty. METHODS: Survey images were digitally modified to create thighs of varying widths and angles relative to fixed buttocks. Thigh-to-buttock ratios and the buttock-thigh junction were studied. Data were stratified and analyzed according to age, gender, and ethnicity of the respondents. Amazon Mechanical Turk was used as a novel crowdsourcing platform for surveying aesthetic preferences. RESULTS: A total of 1034 responses were included of whom 54.4% were male, and 45.6% were female. All age groups and ethnicities were represented. Overall, 43.8% of respondents preferred the widest buttock-thick junction angle on posterior view. There was no clear preference between larger or smaller thigh-to-hip ratios on lateral view. CONCLUSIONS: Characteristics of the ideal thigh include wider thighs with greater horizontal projection, creating a more natural contour from the augmented buttock. These findings represent a paradigm shift from the traditionally assumed preference for slender thighs. Plastic surgeons should carefully consider thigh anatomy in their gluteal augmentation patients, as simultaneous thigh augmentation may lead to a more aesthetically pleasing outcome. Further research is needed into best practices and techniques to attain ideal thigh proportions.


Assuntos
Contorno Corporal/métodos , Nádegas/cirurgia , Estética , Lipectomia/métodos , Coxa da Perna/anatomia & histologia , Tecido Adiposo/transplante , Adulto , Idoso , Nádegas/anatomia & histologia , Crowdsourcing/estatística & dados numéricos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Satisfação do Paciente , Inquéritos e Questionários/estatística & dados numéricos , Coxa da Perna/cirurgia , Adulto Jovem
3.
Aesthet Surg J ; 36(9): 1029-35, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26977072

RESUMO

BACKGROUND: Abdominoplasty is a common aesthetic procedure in the United States. Pollock and Pollock described their progressive tension technique in 2000 and published a series of 597 patients in 2012 of their experience. The reported seroma rate in the literature ranges from 2% to 26% with drains and 0.1% to 4% with progressive tension sutures (PTS) without drains. OBJECTIVES: Given these data, we decided to use PTS and forego drains in abdominoplasty. Here we present our experience with the transition. METHODS: This is a retrospective chart review of 451 abdominoplasties performed at our outpatient surgery center over a 7-year period (2009-2015). We gathered data on patient demographics, concomitant liposuction, and complications and length of follow up. RESULTS: Five main differences were examined in PTS vs traditional abdominoplasty using drains groups. These included rate of seroma, wound complication, scar revision, hematoma, and follow up. We found a decreased rate of seroma in the PTS group, 2% vs 9%. Wound complications were similar. Scar revision was slightly higher in the PTS group at 17% vs 10% in traditional abdominoplasty, this association had a P value of .048. The rates of hematoma were similar (0% vs 1%). The mean follow up was 6 months in PTS and 9 months in traditional abdominoplasty. Addition of liposuction did not increase the rate of seroma. CONCLUSIONS: PTS without drains significantly decreased the seroma rate in our practice. Our experience adds to the mounting evidence that surgeons should consider using the PTS technique and abandon the use of drains in abdominoplasty. A well powered, multicenter, randomized controlled study is needed in order to definitively lay this question to rest. LEVEL OF EVIDENCE: 4 Therapeutic.


Assuntos
Abdominoplastia/métodos , Complicações Pós-Operatórias/prevenção & controle , Seroma/prevenção & controle , Suturas , Abdominoplastia/efeitos adversos , Adulto , Idoso , Drenagem , Feminino , Humanos , Pessoa de Meia-Idade , Estudos Retrospectivos
4.
Aesthet Surg J ; 35(2): 156-64, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25681105

RESUMO

BACKGROUND: Nipple inversion in females can be congenital or acquired. Women who desire treatment for this condition often report difficulty with breastfeeding and interference with their sexuality. However, data are limited on the demographics of patients who undergo surgery to repair inverted nipples and the associated recurrence rates and complications. OBJECTIVES: The authors assessed outcomes of a 7-year experience with an integrated approach to the correction of nipple inversion that minimizes ductal disruption. METHODS: A retrospective chart review was performed for 103 consecutive patients who underwent correction of nipple inversion. (The correction technique was initially reported in 2004 and entailed an integrated approach.) Complication rates, breastfeeding status, and patient demographics were documented. RESULTS: Among the 103 patients, 191 nipple corrections were performed. Nine patients had undergone previous nipple-correction surgery. Recurrence was experienced by 12.6% of patients, 3 of whom had bilateral recurrence. Other complications were partial nipple necrosis (1.05%), breast cellulitis (1.57%), and delayed healing (0.5%). The overall complication rate was 15.74%. Fifty-seven percent of the patients had a B-cup breast size, and 59% were 21 to 30 years of age. CONCLUSIONS: Results of the authors' 7-year experience demonstrate the safety and effectiveness of their technique to correct inverted nipples. LEVEL OF EVIDENCE 4: Therapeutic.


Assuntos
Aleitamento Materno/estatística & dados numéricos , Mamilos/cirurgia , Complicações Pós-Operatórias/epidemiologia , Adolescente , Adulto , Feminino , Humanos , Pessoa de Meia-Idade , Mamilos/anormalidades , Recidiva , Reoperação , Estudos Retrospectivos , Adulto Jovem
5.
Aesthet Surg J ; 35(1): 94-104, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25568240

RESUMO

Numerous surgical options for breast reduction have been described, but in the current healthcare environment, efficiency is of the utmost importance. In this Featured Operative Technique, the authors describe an efficient, reproducible, and simple method for minimal to moderate reduction mammaplasty that utilizes a superior pedicle. The surgical maneuvers were developed and conveyed to the senior author (W.G.S.) by Dr John Bostwick. This approach preserves superior and medial breast fullness while providing appropriate resection of the breast parenchyma to ameliorate symptoms and produce a smaller, lifted breast with a more youthful appearance. The surgical technique maintains a reliable blood supply to the nipple-areola complex (NAC) from the internal mammary artery and its perforators, and involves minimal transposition of the NAC. The authors reviewed the charts of 62 consecutive patients who underwent this procedure and found the complication rate to be 11.3%. Complications included 1 hematoma, 1 standing cone deformity, 3 soft-tissue infections, 8 incisional breakdowns, and 1 unilateral necrosis of the NAC.


Assuntos
Mama/irrigação sanguínea , Mama/cirurgia , Mamoplastia/métodos , Adulto , Feminino , Humanos , Mamoplastia/efeitos adversos , Pessoa de Meia-Idade , Complicações Pós-Operatórias/etiologia , Resultado do Tratamento
7.
Aesthet Surg J ; 34(7): 1018-40, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25168806

RESUMO

Increasing the volume of the breast while simultaneously decreasing the skin envelope equates to surgery involving opposing forces. Increasing patient demand and the evolving perceptions of surgeons have led to the growing popularity of the combined augmentation-mastopexy operation. In turn, secondary augmentation-mastopexies and revisional surgeries of primary augmentation-mastopexies also have increased in popularity. In this article, the authors describe indications for secondary augmentation-mastopexy, techniques for performing this combined procedure safely and effectively, adjunctive procedures, potential pitfalls, and the treatment of complications.


Assuntos
Implante Mamário/efeitos adversos , Implante Mamário/métodos , Mama/irrigação sanguínea , Mama/cirurgia , Remoção de Dispositivo , Complicações Pós-Operatórias/cirurgia , Implante Mamário/instrumentação , Implantes de Mama , Remoção de Dispositivo/efeitos adversos , Feminino , Humanos , Satisfação do Paciente , Seleção de Pacientes , Desenho de Prótese , Reoperação , Medição de Risco , Fatores de Risco , Resultado do Tratamento
8.
Aesthet Surg J ; 34(5): 723-32, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24792479

RESUMO

BACKGROUND: Despite the increasing popularity of the combined augmentation mastopexy procedure among patients, the safety and efficacy of this surgery have been questioned by many surgeons. OBJECTIVE: The authors investigated the safety and efficacy of the combined augmentation mastopexy procedure. METHODS: The authors retrospectively reviewed the medical records of 615 consecutive patients who underwent combined augmentation mastopexy procedures at a single outpatient surgery center from 1992 through 2011. Patient demographics, operative and implant details, and long-term outcomes were analyzed. Rates of complications and revisions were calculated. RESULTS: The most common complications were poor scarring (5.7%), wound-healing problems (2.9%), and deflation of saline implants (2.4%). Of the 615 patients evaluated, 104 (16.9%) elected to undergo revision surgery: 54 revision procedures were secondary to implant-related complications, and 50 were secondary to tissue-related complications. Our data compare favorably with previously reported revision rates for breast augmentation alone and mastopexy alone. CONCLUSIONS: With a skilled surgeon and proper patient selection, the combined augmentation mastopexy procedure can be safe and effective. LEVEL OF EVIDENCE: 4.


Assuntos
Implante Mamário/métodos , Adolescente , Adulto , Idoso , Implante Mamário/efeitos adversos , Implante Mamário/instrumentação , Implantes de Mama , California , Competência Clínica , Feminino , Humanos , Pessoa de Meia-Idade , Seleção de Pacientes , Fotografação , Complicações Pós-Operatórias/etiologia , Complicações Pós-Operatórias/cirurgia , Desenho de Prótese , Estudos Retrospectivos , Fatores de Tempo , Resultado do Tratamento , Adulto Jovem
9.
Ann Surg Oncol ; 20(2): 607-14, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22941163

RESUMO

BACKGROUND: Determining the nature of a breast mass after autologous reconstruction can be difficult. METHODS: A retrospective review of all autologous breast reconstructions was performed over 10 years. All postoperative breast masses were identified. Tumor characteristics, adjuvant treatment, timing of the development of the mass, and correlation with radiology were reviewed. RESULTS: A total of 365 flaps were performed on 272 patients [253 deep inferior epigastric perforator (DIEP), 35 superficial inferior epigastric artery (SIEA), 22 muscle-sparing free transverse rectus abdominis myocutaneous (free MS-TRAM), 25 latissimus, and 30 pedicled TRAM]. Breast masses were identified in 66 breasts (18 %). The majority of these were from fat necrosis, occurring in 54 breasts (15 % overall; DIEP 13.4 %, SIEA 5.7 %, free MS-TRAM 15 %, latissimus 0 %, pedicled TRAM 47 %), first identified at a mean of 3 months. Recurrent carcinoma was diagnosed in 13 breasts (3.6 %). Factors associated with the postreconstruction mass representing recurrent carcinoma were later time period after reconstruction (mean 24 months), closer surgical margins, and lymphovascular invasion. Radiographic imaging accurately diagnosed recurrent carcinoma in 11 (92 %) of 12 patients in whom it was utilized and suggested a benign diagnosis in all 16 patients with fat necrosis in whom it was utilized. CONCLUSIONS: Breast masses frequently present after autologous reconstruction. Fat necrosis is the most common cause. Recurrent carcinoma can occur in the reconstructed breast and presents later. A higher index of suspicion for recurrence should accompany any mass in which prior lymphovascular invasion was present or if original margins were <1 cm. Radiographic imaging accurately identifies the cause of these masses.


Assuntos
Doenças Mamárias/etiologia , Mama/anormalidades , Carcinoma Ductal de Mama/cirurgia , Carcinoma Lobular/cirurgia , Mamoplastia/efeitos adversos , Recidiva Local de Neoplasia/diagnóstico , Complicações Pós-Operatórias , Adulto , Doenças Mamárias/patologia , Doenças Mamárias/cirurgia , Carcinoma Ductal de Mama/complicações , Carcinoma Ductal de Mama/patologia , Carcinoma Intraductal não Infiltrante/complicações , Carcinoma Intraductal não Infiltrante/patologia , Carcinoma Intraductal não Infiltrante/cirurgia , Carcinoma Lobular/complicações , Carcinoma Lobular/patologia , Feminino , Seguimentos , Humanos , Estadiamento de Neoplasias , Prognóstico , Estudos Retrospectivos , Retalhos Cirúrgicos
12.
Am Surg ; 70(10): 932-5, 2004 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-15529855

RESUMO

The purpose of this study is to evaluate the treatment of patients with acute diverticulitis in the inpatient setting using minimal intervention. This was a retrospective study of 75 patients admitted over a 3-year period with acute diverticulitis as evidenced by computed tomography (CT) and clinical scenario. Of the patients enrolled, 24 (32%) had abscesses identified on their initial CT scan. An additional four patients had abscesses noted on a subsequent CT scan obtained because of lack of complete improvement with medical management, thus raising the total number of abscesses to 28 (37%). Of the patients with abscesses, 10 (36%) underwent drainage using a CT-guided percutaneous or ultrasound-guided transrectal approach an average of 6 days after admission. Of the 75 patients, five (7%) required operative intervention during the initial hospitalization for failure of medical management, two (40%) of whom had abscesses on presentation. The overall median length of hospitalization was 5 (interquartile range [IQR] 4-9) days, and 18 patients (24%) had recurrences during the study period. Our conservative approach to percutaneous and surgical intervention resulted in relatively low percutaneous drainage, a low operative rate, and a reasonable length of hospitalization and recurrence rate.


Assuntos
Abscesso Abdominal/terapia , Diverticulite/terapia , Abscesso Abdominal/diagnóstico por imagem , Abscesso Abdominal/etiologia , Doença Aguda , Adulto , Diverticulite/complicações , Diverticulite/diagnóstico por imagem , Drenagem/métodos , Feminino , Hospitalização , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Procedimentos Cirúrgicos Operatórios/métodos , Tomografia Computadorizada por Raios X/métodos
13.
J Burn Care Res ; 35(4): e269-72, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-23811790

RESUMO

Muriatic acid (hydrochloric acid), a common cleaning and resurfacing agent for concrete pools, can cause significant burn injuries. When coating a pool with chlorinated rubber-based paint, the pool surface is initially cleansed using 31.45% muriatic acid. Here we report a 50-year-old Hispanic male pool worker who, during the process of a pool resurfacing, experienced significant contact exposure to a combination of muriatic acid and blue chlorinated rubber-based paint. Confounding the clinical situation was the inability to efficiently remove the chemical secondary to the rubber-based nature of the paint. Additionally, vigorous attempts were made to remove the rubber paint using a variety of agents, including bacitracin, chlorhexidine soap, GOOP adhesive, and Johnson's baby oil. Resultant injuries were devastating fourth-degree burns requiring an immediate operative excision and amputation. Despite aggressive operative intervention and resuscitation, he continued to have severe metabolic derangements and ultimately succumbed to his injuries. We present our attempts at debridement and the system in place to manage patients with complex chemical burns.


Assuntos
Queimaduras Químicas/etiologia , Cáusticos/toxicidade , Cloro/toxicidade , Ácido Clorídrico/toxicidade , Exposição Ocupacional/efeitos adversos , Pintura/toxicidade , Queimaduras Químicas/cirurgia , Cloro/análise , Evolução Fatal , Humanos , Masculino , Pessoa de Meia-Idade , Piscinas
14.
Clin Plast Surg ; 38(2): 241-52, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21620149

RESUMO

Perforator flaps are preferable for breast reconstruction after mastectomy in many patients. Preoperative imaging of the perforators and source vessels is desirable to reduce surgeon stress, limit donor and recipient site complications, and minimize operative time and associated costs. Computed tomographic angiography (CTA) has been shown to provide highly accurate representations of vascular anatomy with excellent spatial resolution. A critical review of the currently available literature was performed to identify the benefits of preoperative imaging (specifically CTA) in perforator flap reconstruction.


Assuntos
Angiografia , Mamoplastia , Retalhos Cirúrgicos/irrigação sanguínea , Tomografia Computadorizada por Raios X , Feminino , Humanos , Retalhos Cirúrgicos/efeitos adversos , Resultado do Tratamento , Ultrassonografia Doppler
15.
Hand (N Y) ; 4(3): 323-6, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19214641

RESUMO

Bowler's thumb presents as paresthesias or a neuroma involving the ulnar digital nerve of the thumb. Over 95 million people enjoy bowling worldwide with nearly 3 million certified league bowlers in the United States. While the incidence of Bowler's thumb is unknown, it is an unrelenting nuisance for bowlers, and symptoms can be severe enough to prevent further sport participation. The condition can be managed nonoperatively with rest and splinting, but successful nonoperative treatment frequently requires discontinuation of bowling. The pressure on athletes to resume sports participation sooner and the possibility of nonoperative treatment failure mandate the need for development of a dependable surgical procedure for this condition. We present a case report of a successful surgical treatment by transposing the ulnar digital nerve dorsal to the adductor pollicis. The patient returned to manual labor and resumed bowling and is symptom free 3 years postsurgery.

16.
Am J Surg ; 196(6): 871-7; discussion 877, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19095102

RESUMO

BACKGROUND: To assess if diagnostic laparoscopy (DL) is superior to nonoperative modes (serial abdominal examination with/without computed axial tomography [CAT] and diagnostic peritoneal lavage) in determining the need for therapeutic laparotomy (TL) after anterior abdominal stab wound (ASW). METHODS: Retrospective review of ASW patients. Patients were divided into group A (DL/exploratory laparotomy) to identify peritoneal violation (PV) and group B (initial nonoperative modes). RESULTS: Seventy-three patients met inclusion criteria. In group A (n = 38), 29 patients (76%) had PV by DL and underwent exploratory laparotomy. Only 10 (35%) underwent TL (sensitivity for PV = 100%; specificity and positive predictive value of PV in determining need for TL = 29% and 33%, respectively). In group B (n = 35), 7 patients (20%) underwent TL, yielding an improved specificity (96%) and positive predictive value (88%). CONCLUSIONS: We find no role for DL in the evaluation of ASW patients solely to determine PV.


Assuntos
Traumatismos Abdominais/diagnóstico , Laparoscopia/estatística & dados numéricos , Ferimentos Perfurantes/diagnóstico , Traumatismos Abdominais/cirurgia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Seguimentos , Humanos , Laparotomia/métodos , Masculino , Pessoa de Meia-Idade , Prognóstico , Reprodutibilidade dos Testes , Estudos Retrospectivos , Índices de Gravidade do Trauma , Ferimentos Perfurantes/cirurgia , Adulto Jovem
17.
Dis Colon Rectum ; 49(2): 183-9, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16322960

RESUMO

PURPOSE: There is no definite consensus on the management of intra-abdominal abscesses in adults. This retrospective study evaluated the use of antibiotic therapy and percutaneous image-guided drainage in adult patients with intra-abdominal abscesses. METHODS: A retrospective chart review of 114 patients with intra-abdominal abscesses was conducted. Data collected included patient demographics, presenting symptoms, radiographic interpretation, vital signs, antibiotic coverage, laboratory values, and details of the hospital course. Bivariate statistical tests were performed using the Wilcoxon rank-sum test, chi-squared test, or Fisher's exact test, where appropriate. RESULTS: Sixty-seven of 114 patients (59 percent) had intra-abdominal abscesses resulting from appendicitis, diverticulitis in 30 patients (26 percent), postoperative in 13 patients (11 percent), and undetermined in 4 patients (4 percent). Three patients (3 percent; 95 percent confidence interval, 1-8 percent) failed conservative management and underwent urgent operation. Sixty-one (54 percent; 95 percent confidence interval, 44-63 percent) patients improved with intravenous antibiotic therapy alone. Fifty patients (44 percent; 95 percent confidence interval, 35-54 percent) underwent image-guided percutaneous drainage after 48 to 72 hours of antibiotic therapy. Patients who improved on antibiotics alone had average abscess diameter of 4 cm, whereas patients who underwent percutaneous drainage had average diameter of 6.5 cm (P<0.0001). Maximal temperature at time of admission was 100.8 degrees F for antibiotic group and 101.2 degrees F for percutaneous drainage group (P=0.0067). CONCLUSIONS: The majority of the patients with intra-abdominal abscesses improved with antibiotic therapy alone. Those patients with an abscess diameter>6.5 cm and temperature at admission>101.2 degrees F have higher likelihood of failing conservative therapy with antibiotics alone and requiring percutaneous drainage.


Assuntos
Abscesso Abdominal/terapia , Antibacterianos/uso terapêutico , Drenagem/métodos , Abscesso Abdominal/tratamento farmacológico , Adulto , Estudos de Coortes , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Radiografia Intervencionista , Estudos Retrospectivos , Tomografia Computadorizada por Raios X , Resultado do Tratamento , Ultrassonografia de Intervenção
18.
Proc Natl Acad Sci U S A ; 100(9): 5342-7, 2003 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-12707414

RESUMO

It is not clear why the N-terminal autoantigenic determinant of myelin basic protein (MBP), Ac1-9, is dominant in the B1O.PL (H-2(u)) mouse, given its weak I-A(u)-MHC binding affinity. Similarly, how do high-affinity T cells specific for this determinant avoid negative selection? Because the MBP:1-9 sequence is embryonically expressed uniquely in the context of Golli-MBP, determinants were sought within the contiguous N-terminal "Golli" region that could out-compete MBP:1-9 for MHC binding, and thereby prevent negative selection of the public response to Ac1-9, shown here to be comprised of a V beta 8.2J beta 2.7 and a V beta 8.2J beta 2.4 expansion. Specifically, we demonstrate that Ac1-9 itself can be an effective inducer of central tolerance induction; however, in the context of Golli-MBP, Ac1-9 is flanked by determinants which prevent its display to autoreactive T cells. Our data support competitive capture as a means of protecting high-affinity, autoreactive T cells from central tolerance induction.


Assuntos
Antígenos de Histocompatibilidade Classe II/imunologia , Tolerância Imunológica , Linfócitos T/imunologia , Animais , Camundongos , Camundongos Knockout
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