Biofabrication of Fe3O4 Nanoparticles from Spirogyra hyalina and Ajuga bracteosa and Their Antibacterial Applications.

Iron oxide nanoparticles (NPs) have attracted substantial interest due to their superparamagnetic features, biocompatibility, and nontoxicity. The latest progress in the biological production of Fe3O4 NPs by green methods has improved their quality and biological applications significantly. In this study, the fabrication of iron oxide NPs from Spirogyra hyalina and Ajuga bracteosa was conducted via an easy, environmentally friendly, and cost-effective process. The fabricated Fe3O4 NPs were characterized using various analytical methods to study their unique properties. UV-Vis absorption peaks were observed in algal and plant-based Fe3O4 NPs at 289 nm and 306 nm, respectively. Fourier transform infrared (FTIR) spectroscopy analyzed diverse bioactive phytochemicals present in algal and plant extracts that functioned as stabilizing and capping agents in the fabrication of algal and plant-based Fe3O4 NPs. X-ray diffraction of NPs revealed the crystalline nature of both biofabricated Fe3O4 NPs and their small size. Scanning electron microscopy (SEM) revealed that algae and plant-based Fe3O4 NPs are spherical and rod-shaped, averaging 52 nm and 75 nm in size. Energy dispersive X-ray spectroscopy showed that the green-synthesized Fe3O4 NPs require a high mass percentage of iron and oxygen to ensure their synthesis. The fabricated plant-based Fe3O4 NPs exhibited stronger antioxidant properties than algal-based Fe3O4 NPs. The algal-based NPs showed efficient antibacterial potential against E. coli, while the plant-based Fe3O4 NPs displayed a higher zone of inhibition against S. aureus. Moreover, plant-based Fe3O4 NPs exhibited superior scavenging and antibacterial potential compared to the algal-based Fe3O4 NPs. This might be due to the greater number of phytochemicals in plants that surround the NPs during their green fabrication. Hence, the capping of bioactive agents over iron oxide NPs improves antibacterial applications.

Synergistic Influence of Yeast Extract and Calcium Oxide Nanoparticles on the Synthesis of Bioactive Antioxidants and Metabolites in Swertia chirata In Vitro Callus Cultures.

The challenges in the production of metabolites of medicinal potential from wild plants include low yields, slow growth rates, seasonal variations, genetic variability and regulatory as well as ethical constraints. Overcoming these challenges is of paramount significance and interdisciplinary approaches and innovative strategies are prevalently applied to optimize phytoconstituents' production, enhance yield, biomass, ensure sustainable consistency and scalability. In this study, we investigated the effects of elicitation with yeast extract and calcium oxide nanoparticles (CaONPs) on in vitro cultures of Swertia chirata (Roxb. ex Fleming) Karsten. Specifically, we examined the effects of different concentrations of CaONPs in combination with different concentrations of yeast extract on various parameters related to callus growth, antioxidant activity, biomass and phytochemical contents. Our results showed that elicitation with yeast extract and CaONPs had significant effects on the growth and characteristics of callus cultures of S. chirata. The treatments involving yeast extract and CaONPs were found to be the most effective in increasing the contents of total flavonoid contents (TFC), total phenolic contents (TPC), amarogentin and mangiferin. These treatments also led to an improvement in the contents of total anthocyanin and alpha tocopherols. Additionally, the DPPH scavenging activity was significantly increased in the treated samples. Furthermore, the treatments involving elicitation with yeast extract and CaONPs also led to significant improvements in callus growth and characteristics. These treatments promoted callus response from an average to an excellent level and improved the color and nature of the callus from yellow to yellow-brown and greenish and from fragile to compact, respectively. The best response was observed in treatments involving 0.20 g/L yeast extract and 90 ug/L CaONPs. Overall, our findings suggest that elicitation with yeast extract and CaONPs can be a useful strategy for promoting the growth, biomass, phytochemical contents and antioxidant activity of callus cultures of S. chirata in comparison to wild plant herbal drug samples.

Supersaturation-Based Drug Delivery Systems: Strategy for Bioavailability Enhancement of Poorly Water-Soluble Drugs.

At present, the majority of APIs synthesized today remain challenging tasks for formulation development. Many technologies are being utilized or explored for enhancing solubility, such as chemical modification, novel drug delivery systems (microemulsions, nanoparticles, liposomes, etc.), salt formation, and many more. One promising avenue attaining attention presently is supersaturated drug delivery systems. When exposed to gastrointestinal fluids, drug concentration exceeds equilibrium solubility and a supersaturation state is maintained long enough to be absorbed, enhancing bioavailability. In this review, the latest developments in supersaturated drug delivery systems are addressed in depth.

Fruit Bromelain-Derived Peptide Potentially Restrains the Attachment of SARS-CoV-2 Variants to hACE2: A Pharmacoinformatics Approach.

Before entering the cell, the SARS-CoV-2 spike glycoprotein receptor-binding domain (RBD) binds to the human angiotensin-converting enzyme 2 (hACE2) receptor. Hence, this RBD is a critical target for the development of antiviral agents. Recent studies have discovered that SARS-CoV-2 variants with mutations in the RBD have spread globally. The purpose of this in silico study was to determine the potential of a fruit bromelain-derived peptide. DYGAVNEVK. to inhibit the entry of various SARS-CoV-2 variants into human cells by targeting the hACE binding site within the RBD. Molecular docking analysis revealed that DYGAVNEVK interacts with several critical RBD binding residues responsible for the adhesion of the RBD to hACE2. Moreover, 100 ns MD simulations revealed stable interactions between DYGAVNEVK and RBD variants derived from the trajectory of root-mean-square deviation (RMSD), radius of gyration (Rg), and root-mean-square fluctuation (RMSF) analysis, as well as free binding energy calculations. Overall, our computational results indicate that DYGAVNEVK warrants further investigation as a candidate for preventing SARS-CoV-2 due to its interaction with the RBD of SARS-CoV-2 variants.

Immunoadjuvant and Humoral Immune Responses of Garlic (Allium sativum L.) Lectins upon Systemic and Mucosal Administration in BALB/c Mice.

Dietary food components have the ability to affect immune function; following absorption, specifically orally ingested dietary food containing lectins can systemically modulate the immune cells and affect the response to self- and co-administered food antigens. The mannose-binding lectins from garlic (Allium sativum agglutinins; ASAs) were identified as immunodulatory proteins in vitro. The objective of the present study was to assess the immunogenicity and adjuvanticity of garlic agglutinins and to evaluate whether they have adjuvant properties in vivo for a weak antigen ovalbumin (OVA). Garlic lectins (ASA I and ASA II) were administered by intranasal (50 days duration) and intradermal (14 days duration) routes, and the anti-lectin and anti-OVA immune (IgG) responses in the control and test groups of the BALB/c mice were assessed for humoral immunogenicity. Lectins, co-administered with OVA, were examined for lectin-induced anti-OVA IgG response to assess their adjuvant properties. The splenic and thymic indices were evaluated as a measure of immunomodulatory functions. Intradermal administration of ASA I and ASA II had showed a four-fold and two-fold increase in anti-lectin IgG response, respectively, vs. the control on day 14. In the intranasal route, the increases were 3-fold and 2.4-fold for ASA I and ASA II, respectively, on day 50. No decrease in the body weights of animals was noticed; the increases in the spleen and thymus weights, as well as their indices, were significant in the lectin groups. In the adjuvanticity study by intranasal administration, ASA I co-administered with ovalbumin (OVA) induced a remarkable increase in anti-OVA IgG response (~six-fold; p < 0.001) compared to the control, and ASA II induced a four-fold increase vs. the control on day 50. The results indicated that ASA was a potent immunogen which induced mucosal immunogenicity to the antigens that were administered intranasally in BALB/c mice. The observations made of the in vivo study indicate that ASA I has the potential use as an oral and mucosal adjuvant to deliver candidate weak antigens. Further clinical studies in humans are required to confirm its applicability.

Secondary Metabolite Profiling, Anti-Inflammatory and Hepatoprotective Activity of Neptunia triquetra (Vahl) Benth.

The present study aimed to analyze the phytoconstituents of Neptunia triquetra (Vahl) Benth. Anti-inflammatory and hepatoprotective activities of ethanol (EE), chloroform (CE) and dichloromethane (DCME) of stem extracts were evaluated using in vivo experimental models. The extracts were analyzed for phytoconstituents using GC-HRMS. Anti-inflammatory activity of CE, EE and DCME was accessed using carrageenan-induced paw oedema, cotton pellet-induced granuloma and the carrageenan-induced air-pouch model in Wistar albino rats. The hepatotoxicity-induced animal models were investigated for the biochemical markers in serum (AST, ALT, ALP, GGT, total lipids and total protein) and liver (total protein, total lipids, GSH and wet liver weight). In the in vivo study, animals were divided into different groups (six in each group) for accessing the anti-inflammatory and hepatoprotective activity, respectively. GC-HRMS analysis revealed the presence of 102 compounds, among which 24 were active secondary metabolites. In vivo anti-inflammatory activity of stem extracts was found in the order: indomethacin > chloroform extract (CE) > dichloromethane extract (DCME) > ethanolic extract (EE), and hepatoprotective activity of stem extracts in the order: CE > silymarin > EE > DCME. The results indicate that N. triquetra stem has a higher hepatoprotective effect than silymarin, however the anti-inflammatory response was in accordance with or lower than indomethacin.

Production of Verbascoside, Isoverbascoside and Phenolic Acids in Callus, Suspension, and Bioreactor Cultures of Verbena officinalis and Biological Properties of Biomass Extracts.

Callus, suspension and bioreactor cultures of Verbena officinalis were established, and optimized for biomass growth and production of phenylpropanoid glycosides, phenolic acids, flavonoids and iridoids. All types of cultures were maintained on/in the Murashige and Skoog (MS) media with 1 mg/L BAP and 1 mg/L NAA. The inoculum sizes were optimized in callus and suspension cultures. Moreover, the growth of the culture in two different types of bioreactors-a balloon bioreactor (BB) and a stirred-tank bioreactor (STB) was tested. In methanolic extracts from biomass of all types of in vitro cultures the presence of the same metabolites-verbascoside, isoverbascoside, and six phenolic acids: protocatechuic, chlorogenic, vanillic, caffeic, ferulic and rosmarinic acids was confirmed and quantified by the HPLC-DAD method. In the extracts from lyophilized culture media, no metabolites were found. The main metabolites in biomass extracts were verbascoside and isoverbascoside. Their maximum amounts in g/100 g DW (dry weight) in the tested types of cultures were as follow: 7.25 and 0.61 (callus), 7.06 and 0.48 (suspension), 7.69 and 0.31 (BB), 9.18 and 0.34 (STB). The amounts of phenolic acids were many times lower, max. total content reached of 26.90, 50.72, 19.88, and 36.78 mg/100 g DW, respectively. The highest content of verbascoside and also a high content of isoverbascoside obtained in STB (stirred-tank bioreactor) were 5.3 and 7.8 times higher than in extracts from overground parts of the parent plant. In the extracts from parent plant two iridoids-verbenalin and hastatoside, were also abundant. All investigated biomass extracts and the extracts from parent plant showed the antiproliferative, antioxidant and antibacterial activities. The strongest activities were documented for the cultures maintained in STB. We propose extracts from in vitro cultured biomass of vervain, especially from STB, as a rich source of bioactive metabolites with antiproliferative, antioxidant and antibacterial properties.

Mammillaria Species-Polyphenols Studies and Anti-Cancer, Anti-Oxidant, and Anti-Bacterial Activities.

Discovering new natural resources of polyphenols is the aim of many recent studies in the field of natural product research. This study tentatively investigated the polyphenols profile of the stems of seven Mammillaria species (M. rhodantha, M. spinosissima, M. hahniana, M. crucigera, M. candida, M. albilanata, and M. muehlenpfordtii) using high performance liquid chromatography with DAD detector (HPLC-DAD) method. Furthermore, the anti-cancer, anti-oxidant, and anti-bacterial potentials of these extracts as well as major identified phenols were explored. The HPLC-DAD study confirmed the availability of six phenolic acids, including gentisic acid, chlorogenic acid, caffeic acid, protocatechuic acid, sinapic acid, and p-hydroxybenzoic acid. The dominant compounds were: gentisic acid in M. rhodantha and M. spinosissima; chlorogenic acid in M. muehlenpfordtii, M. crucigera, and M. rhodantha; and caffeic acid in M. rhodantha, M. crucigera, and M. spinosissima. Stems of Mammillaria sp. showed antiproliferative effects against HeLa, MCF-7, and Jurkat cells. In HeLa and MCF-7 cells, the best antiproliferative activities were found in the treatments with M. rhodantha, M. spinosissima, and M. muehlenpfordtii. The apoptotic assay of M. rhodantha, M. spinosissima, and M. muehlenpfordtii showed accumulation of necrotic cells in the early and late apoptotic phase. M. rhodantha, M. spinosissima, and M. muehlenpfordtii showed the highest anti-oxidant activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ß-carotene bleaching, and ferric reducing anti-oxidant power (FRAP) assays. M. rhodantha was the best source of antioxidants. Mammillaria sp. showed moderate anti-bacterial effects against bacteria and the highest effects were found using the extracts of M. rhodantha, M. spinosissima, M. crucigera and M. muehlenpfordtii against most bacteria. The anti-bacterial activities were attributed to other phenolic compounds (e.g., chlorogenic acid) than gentisic acid, which was not active against most bacteria. Mammillaria sp. could be considered to be an important natural source of phenolic acids with anti-cancer, anti-bacterial, and anti-oxidant activities.

Phenolic Compounds of Catalpa speciosa, Taxus cuspidate, and Magnolia acuminata have Antioxidant and Anticancer Activity.

Tree bark represents an important source of medicinal compounds that may be useful for cancer therapy. In the current study, high-performance liquid chromatography with diode-array detection (HPLC-DAD) was used to determine the profile of the phenolic compounds of Catalpa speciosa, Taxus cuspidata, and Magnolia acuminata bark extracts. The antioxidant and anticancer bioactivities against different cancer cell lines were investigated. M. acuminata exerted significantly higher antioxidant activities in the diphenyl picrylhydrazine and ß-carotene-linoleic acid assays than the other species. In C. speciosa, novel profiles of phenolic acids (ferulic acid was the predominant compound) and catechin were detected. In T. cuspidata, six phenolic acids were detected; the predominant compounds were hydroxycaffeic acid and protocatechuic acid. In M. acuminata, two phenolic acids and three catechins were detected; catechin was the predominant compound. The three species exerted clear anticancer activity against MCF-7, HeLa, Jurkat, T24, and HT-29 cells, with the strongest activity found in the extracts from M. acuminata. No antiproliferative activity against normal cells was found. Flow cytometry revealed greater accumulation of necrotic and early/late apoptotic cells in various treated cancer cells than in untreated control cells, and protocatechuic acid induced a similar accumulation of necrotic cells to that of the bark extracts. Caspase-3 and -7 activity was increased in cancer cells treated with different bark extracts; the highest activity was found in the M. acuminata treatment. Our results suggested that the treatment of cancer cells with bark extracts of M. acuminata, C. speciosa, and T. cuspidata, and protocatechuic acid induced apoptosis, suggesting an association between anticancer activities and individual phenolic compounds.

Effective antioxidant, antimicrobial and anticancer activities of essential oils of horticultural aromatic crops in northern Egypt.

BACKGROUND: Identifying ornamental plants as new natural antioxidant and antimicrobial sources is always of great importance for the ornamental and horticultural industries. METHODS: The antimicrobial activities of leaves and fruits peel essential oils of twelve ornamental and horticultural crops were determined by screening against wide spectrum of fungi and bacteria, and their respective in vitro antioxidant capacity was evaluated. Furthermore, the anticancer activities against several cancer cells, and one normal human cell line (HEK-293) were examined. RESULTS: Origanum vulgare L. essential oil showed the best antioxidant, antibacterial and anticancer activities compared to screened crops by means of the DPPH and linoleic acid assays for antioxidants, MIC and MBC values for antibacterial activities and IC50 for antiproliferative activities. Such important activities in O. vulgare was attributed to high pulegone ratio (77.45%) as revealed by the GC/MS assay. Rosmarinus officinallis L. essential oil showed the highest antifungal activities by means of lowest MIC and MFC values which might be attributed to 1, 8-cineole (19.60%), camphor (17.01%) and α-pinene (15.12%). CONCLUSION: We suggest that oxygenated monoterpenes (i.e. linalool, terpinen-4-ol and pulegone) and monoterpene hydrocarbons play an important role in the essential oil antioxidant, antibacterial, antifungal and anticancer activities of diverse Egyptian ornamental and horticultural crops. Some species showed bioactivities similar to standards compounds and might be suitable for pharmaceutical and food industries.

Enhancement of Calibrachoa growth, secondary metabolites and bioactivity using seaweed extracts.

BACKGROUND: Calibrachoa x hybrida (Solanaceae) cultivars are widely used in North and South America as ornamental plants. Their potential as a source of antimicrobial compounds might be enhanced by seaweed extract (SWE) applications. METHODS: SWE of Ascophyllum nodosum were applied at 5 and 7 ml/L as a soil drench or foliar spray on Calibrachoa cultivars of Superbells® 'Dreamsicle' (CHSD) and Superbells® 'Frost Fire' (CHSF). The total phenolics, tannins and antioxidants composition as well as specific flavonols in leaf extracts were determined. Further, the chemical composition of SWE was assessed. RESULTS: The drench and foliar SWE treatments significantly enhanced Calibrachoa cultivars leaf number and area, dry weight, plant height, antioxidant capacity as well as phenolic, flavonols and tannin content. The increased growth and composition of phenols, flavonols and tannins was attributed to the stimulatory effects of SWE mineral composition. The antifungal activity of Calibrachoa cultivars was significantly enhanced following SWE treatments and the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were in the range of 0.07-0.31 mg/ml and from 0.16 to 0.56 mg/ml, respectively. Moreover, antibacterial activity was significantly increased and the MIC and minimum bactericidal concentration (MBC) measurements were in the range of 0.06-0.23 mg/ml and from 0.10 to 0.44 mg/ml, respectively. The most sensitive fungus to SWE treatments was C. albicans and the most sensitive bacterium was E. cloacae. CONCLUSION: The results suggest that enhanced antifungal and antibacterial activities might be attributed to significant increases of phenolic, flavonols and tannin contents, which ultimately enhance the potential of Calibrachoa as a natural source of alternative antibiotics.

Magnesium nanoparticles extirpate salt stress in carrots (Daucus carota L.) through metabolomics regulations.

Underground vegetables are sensitive and vulnerable to salt stress. The vegetables are the main source of vitamins, nutrients and minerals in human diet. Also contain healthy carbohydrates, antioxidant and resistant starch which are beneficial for human health. Salinity influences water balance, morphological appearance and cellular interference of crop plants. It also caused disproportion of nutrients which usually affects the physiochemical processes in plant. Salt stress also affect biochemical attributes and hampers the growth of underground organs, due to which yield of crop decreased. The nanoparticles had been potentially used for better crop yield, in the recent. In our research study, we elaborate the positive response of magnesium oxide nanoparticles (MgO-NPs) on the morphological and biochemical parameters as well as anti-oxidant enzymes action on two accessions of carrot (Daucus carota L.) under salt stress of 40 mM and 80 mM. In a pilot experiment, various levels (0, 50, 100, 150, 200 and 250 mg/L) of MgO-NPs were tested through foliar application on carrot plants. Foliar application of MgO-NPs at concentration of 150 mg/L was most effective treatment and ameliorate the salt stress in both carrot accessions (DC-03 and DC-90). The MgO-NPs significantly enhanced the morphological and biochemical parameters. The yield was significantly increased with the exposure of MgO-NPs. Our results thus confirmed the potential of MgO-NPs to endorse the plant development and growth under salinity. However, further research study is needed to explore effectiveness of MgO-NPs in various other plants for the ameliorant of salinity.

Rice residue management alternatives in rice-wheat cropping system: impact on wheat productivity, soil organic carbon, water and microbial dynamics.

In the Indo-Gangetic Plains (IGP), rice-wheat cropping system (RWCS) predominates, producing large quantity of crop residue and its management is major concern. Farmers usually burn the residue to clear the field for succeding crop, and burning damages soil microbes, resulted in loss of soil organic matter. Hence, current study was conducted to assess the impact of different Happy seeder based residue management options on changes in microbial dynamics, enzyme activities and soil organic matter content and also to know that alternative method for attaining sustainable wheat productivity in sandy loam soils of Haryana, India. Results revealed that Zero tillage wheat (ZTW) with partial and full residue retention treatments sown with Happy seeder (after using chopper and spreader), and ZTW with anchored stubbles significantly enhanced soil microbial count by 47.9-60.4%, diazotropic count by 59.0-73.1% and actinomycetes count by 47.3-55.2%, grain yield by 9.8-11.3% and biomass yield by 7.4-9.6% over conventional tilled (CT) residue burning and residue removal plots. ZTW sown with surface retention of rice crop residue increased the organic carbon by 0.36-0.42% and the soil moisture content by 13.4-23.6% over CTW without residue load. Similarly, ZTW sown with Happy seeder with full residue enhanced alkaline phosphatase activity from 95.3 µg TPF g-1 soil 24 h-1 in 2018-2019 to 98.6 µg TPF g-1 soil 24 h-1 in 2019-2020 over control plots. Likely, microbial population and enzymatic activity showed strong positive correlation under variable residue retention practices. However, increased microbial population reduced the soil pH from 7.49 to 7.27 under ZTW with residue retention plots. The wheat yield enhanced by 9.8-11.3% during 2018-2019 and 2019-2020 under ZTW with Happy seeder with full residue load over residue burning and residue removal plots. ZTW sown with Happy seeder under full residue retention, achieved maximum net return 43.16-57.08 × 103 ₹ ha-1) and B-C ratio (1.52 to 1.70) over CTW without residue. Therefore, rice residue needs to be managed by planting wheat using appropriate machinery under ZT for sustaining higher productivity in RWCS and improve soil health and environment under IGP regions.

Transgenerational Seed Exposure to Elevated CO2 Involves Stress Memory Regulation at Metabolic Levels to Confer Drought Resistance in Wheat.

Drought is the worst environmental stress constraint that inflicts heavy losses to global food production, such as wheat. The metabolic responses of seeds produced overtransgenerational exposure to e[CO2] to recover drought's effects on wheat are still unexplored. Seeds were produced constantly for four generations (F1 to F4) under ambient CO2 (a[CO2], 400 µmol L-1) and elevated CO2 (e[CO2], 800 µmol L-1) concentrations, and then further regrown under natural CO2 conditions to investigate their effects on the stress memory metabolic processes liable for increasing drought resistance in the next generation (F5). At the anthesis stage, plants were subjected to normal (100% FC, field capacity) and drought stress (60% FC) conditions. Under drought stress, plants of transgenerational e[CO2] exposed seeds showed markedly increased superoxide dismutase (16%), catalase (24%), peroxidase (9%), total antioxidants (14%), and proline (35%) levels that helped the plants to sustain normal growth through scavenging of hydrogen peroxide (11%) and malondialdehyde (26%). The carbohydrate metabolic enzymes such as aldolase (36%), phosphoglucomutase (12%), UDP-glucose pyrophosphorylase (25%), vacuolar invertase (33%), glucose-6-phosphate-dehydrogenase (68%), and cell wall invertase (17%) were decreased significantly; however, transgenerational seeds produced under e[CO2] showed a considerable increase in their activities in drought-stressed wheat plants. Moreover, transgenerational e[CO2] exposed seeds under drought stress caused a marked increase in leaf Ψw (15%), chlorophyll a (19%), chlorophyll b (8%), carotenoids (12%), grain spike (16%), hundred grain weight (19%), and grain yield (10%). Hence, transgenerational seeds exposed to e[CO2] upregulate the drought recovery metabolic processes to improve the grain yield of wheat under drought stress conditions.

Ethnopharmacological uses of fauna among the people of central Punjab, Pakistan.

Introduction: The utilization of fauna and fauna-based byproducts in ethnomedicinal usages has been a longstanding human activity, practiced across various cultures worldwide. This study focuses on investigating the utilization of animal-based traditional medicine by the people of Pakistan, specifically in the Gujranwala area. Methods: Data collection took place from January to September 2019 through interviews with local communities. Ethnomedicinal applications of animal products were analyzed using several indices, including Relative Frequency of Citation (RFC), Relative Popularity Level (RPL), Folk Use Value (FL), and Relative Occurrence Percentage (ROP). Results: The study identified the use of different body parts of 54 species of animals in treating various diseases and health issues. These include but are not limited to skin infections, sexual problems, pain management (e.g., in the backbone and joints), eyesight issues, immunity enhancement, cold, weakness, burns, smallpox, wounds, poisoning, muscular pain, arthritis, diabetes, fever, epilepsy, allergies, asthma, herpes, ear pain, paralysis, cough, swelling, cancer, bronchitis, girls' maturity, and stomach-related problems. Certain species of fauna were noted by informers with high "frequency of citation" (FC), ranging from 1 to 77. For instance, the black cobra was the most frequently cited animal for eyesight issues (FC = 77), followed by the domestic rabbit for burn treatment (FC = 67), and the Indus Valley spiny-tailed ground lizard for sexual problems (FC = 66). Passer domesticus and Gallus gallus were noted to have the highest ROP value of 99. Discussion: The findings of this study provide valuable preliminary insights for the conservation of fauna in the Gujranwala region of Punjab, Pakistan. Additionally, screening these animals for medicinally active compounds could potentially lead to the development of novel animal-based medications, contributing to both traditional medicine preservation and modern pharmaceutical advancements.

Traditional Wild Food Plants Gathered by Ethnic Groups Living in Semi-Arid Region of Punjab, Pakistan.

Wild edible food plants (WFPs) are valuable resources in the traditional food systems of many local cultures worldwide, particularly in underdeveloped regions. Understanding patterns of food preferences requires conducting cross-cultural food studies among various ethnic groups in a specific area. In this context, the current study aimed to record WFP use among five ethnic groups in Punjab, Pakistan, by interviewing 175 informants selected through snowball sampling. The indicator food species for different ethnic groups were calculated using indicator analysis based on the percentage of citations. A total of 71 wild food plants (WFPs) belonging to 57 genera and 27 families were observed in the study area. A high proportion of these wild food plants (WFPs) belonged to Fabaceae with eleven species (15%), followed by Moraceae with seven species (9%). Fruits were most widely used (43%), followed by leaves (19%), and shoots (16%). The majority (35 species, 49%) of plants of WFPs were eaten as cooked vegetables. A cross-cultural comparison revealed that four species overlapped among five ethnic groups (Arain, Jutt, Rajpot, Mewati, and Dogar). The Arain ethnic group gathered and consumed a remarkable number of wild plants (35 species), possibly due to a special connection with the general abundance of the local flora, and being close to nature by adopting professions more allied to WFPs in the study area. The analysis of indicator species revealed distinct significant indicator values (p ≤ 0.05) between the main food species among the various ethnic groups. Amaranthus viridis was a common indicator of food in all five ethnic groups, while Ziziphus nammularia was a common indicator food plant of the Mewati, Rajpot, and Jutt ethnic groups; these plants are important in local diets, especially during times of food scarcity brought on by disease or drought. In addition, the current study reports 20 WFPs that have been rarely documented as human food in Pakistan's ethnobotanical literature. Future development plans should consider biocultural heritage and pay appropriate attention to local ecological knowledge, dynamics, and historical exchanges of traditional food systems.

Phenology and Diversity of Weeds in the Agriculture and Horticulture Cropping Systems of Indian Western Himalayas: Understanding Implications for Agro-Ecosystems.

Weeds are a major threat to agriculture and horticulture cropping systems that reduce yield. Weeds have a better ability to compete for resources compared to the main crops of various agro-ecosystems and act as a major impediment in reducing overall yield. They often act as energy drains in the managed agroecosystems. We studied weed infestation for five different agro-ecosystems in the part of Indian Western Himalayas represented by paddy, maize, mustard, apple and vegetable orchards. Systematic random sampling was done to record flowering phenology and diversity of weeds during the assessment period 2015-2020. We recorded 59 weed species, taxonomically distributed among 50 genera in 24 families. The Asteraceae family has the most species (15% species), followed by Poaceae (14% species), and Brassicaceae (12% species). The Therophytes were the dominant life form followed by Hemicryptophytes. The majority of the weeds were shown to be at their most blooming in the summer (predominantly from June to July). The Shannon index based diversity of weeds ranged from 2.307-3.325 for the different agro-ecosystems. The highest number of weeds was in the horticulture systems (apple > vegetable) followed by agriculture fields (maize > paddy > mustard). Agriculture and horticulture cropping systems were distinguished using indicator species analysis, which was supported by high and significant indicator values for a number of species. Persicaria hydropiper, Cynodon dactylon, Poa annua, Stellaria media, and Rorippa palustris had the highest indicator value in agriculture cropping systems, while Trifolium repens, Phleum pratense, and Trifolium pratense had the highest indicator value in horticulture cropping systems. We found that eleven weed species were unique to apple gardens followed by nine in maize, four in vegetables, two in mustard and one in paddy fields. Spatial turnover (ßsim) and nestedness-resultant components (ßsne) of species dissimilarity revealed dissimilarity lower than 50% among the five cropping systems. The study is expected to assist in formulating an appropriate management strategy for the control of weed infestation in the study region.

Keeping Healthy in Your Skin-Plants and Fungi Used by Indigenous Himalayan Communities to Treat Dermatological Ailments.

Dermatological ailments are a major health problem, especially when related to human immune deficiency syndrome and acquired immune deficiency. The goal of this study was to identify the medicinal plants used by the indigenous peoples of the Northwestern Himalayas to treat dermatological diseases. Several field trips were conducted in the spring and summer seasons of 2020-2021 to collect the plants of dermatological value and information about their use through open-ended semi-structured interviews (n = 53) and group discussions (n = 33). The current investigation found 64 ethnomedicinal plants belonging to 34 families commonly used to treat a variety of dermatological ailments. The main growth form was herbs (80%), followed by trees (8%) and ferns (6%). It was found that leaves (51%) were the most commonly used plant part, followed by roots and the whole plant. Wound healing was the most dominant application, with 18 plant species used, followed by skin burns cured by 11 plant species and skin boils by eight plant species. Out of the total (18%) of medicinal plants with cosmetic uses, i.e., roots of Jurinea dolomiaea, Rheum webbianum, and Rheum spiciforme were crushed into powder and mixed with turmeric, and the paste is applied topically for glowing skin. Among the various preparation methods, paste (38%) was the most common way of preparation, followed by poultice (29%) and infusion (9%). Between ethnic groups, the maximum homogeneity was between Gujjar and Bakarwal ethnic groups (23 species, 36%), followed by Gujjars and Kashmiri (14 species, 22%). Bakarwals and Gujjar people live in the same geographical location, and they graze their animals in pastures, practice extensive transhumance pastoralism, and pass through different ecological landscapes, thus having sufficient experiences with certain plants and retaining more knowledge. The species identified with the highest utilization based on the number of citations and use value included Ficus carica, Cichorium intybus, Euphorbia wallichii, Pinus wallichiana, Plantago major, Jurinea dolomiaea, and Artemisia absinthium. The findings of this study demonstrate that people who reside in the Northwestern Himalayas region still rely on medicinal plants.

Promising Bioregulators for Higher Water Productivity and Oil Quality of Chia under Deficit Irrigation in Semiarid Regions.

Appropriate water management practices are essential for the successful cultivation of chia in water-scarce situations of semiarid regions. This is highly essential when new crops such as chia are introduced for ensuring diversity and water saving. Therefore, field trials (2020-21 and 2021-22) were conducted to understand the impact of deficit irrigation and bioregulators (BRs) on the seed yield, water productivity, and oil quality of chia. The effect of foliar application of BRs such as thiourea (TU; 400 ppm), salicylic acid (SA; 1.0 mM), potassium nitrate (KN; 0.15%), potassium silicate (KS; 100 ppm), kaolin (KO; 5%), and sodium benzoate (SB; 200 ppm) were monitored at different levels of irrigation: 100 (I100), 75 (I75), 50 (I50), and 25 (I25) percent of cumulative pan evaporation (CPE). Deficit irrigation at I25, I50, and I75 led to 55.3, 20.1, and 3.3% reductions in seed yield; 42.5, 22.5, and 4.2% in oil yield; and 58.9, 24.5, and 5.7% in omega-3 yield, respectively, relative to I100. Bioregulators could reduce the adverse impact of water deficit stress on seed, oil, and omega-3 yield. However, their beneficial effect was more conspicuous under mild water stress (I75), as revealed by higher seed yield (4.3-6.9%), oil yield (4.4-7.1%), and omega-3 yield (4.7-8.5%) over control (I100 + no BRs). Further, BRs (KN, TU, and SA) maintained oil quality in terms of linolenic acid and polyunsaturated fatty acid contents, even under mild stress (I75). Foliar application of KN, TU, and SA could save water to an extent of 36-40%. Therefore, the adverse impact of deficit irrigation on seed, oil, and omega-3 yields of chia could be minimized using BRs such as KN, TU, and SA, which can also contribute to improved water productivity.

Pantoea conspicua promoted sunflower growth and engulfed rhizospheric arsenate by secreting exopolysaccharide.

A rhizobacterium, Pantoea conspicua, was examined against sunflower seedlings' growth under arsenate stress. Sunflower upon exposure to arsenate resulted in compromised growth that might be due to the accumulation of higher concentrations of arsenate and reactive oxygen species (ROS) in seedlings' tissues. The deposited arsenate led to oxidative damage and electrolyte leakage, making the sunflower seedlings vulnerable to compromise its growth and development. However, inoculation of sunflower seedlings with P. conspicua alleviated arsenate stress in host by initiating a multilayered defence mechanism. In fact, P. conspicua filtered out 75.1% of the arsenate from growth medium that were available to the plant roots in the absence of the said strain. To accomplish such activity, P. conspicua secreted exopolysaccharides as well as altered lignification in host roots. The arsenate (24.9%) that made its way to plant tissues was countered by helping the host seedlings to produce higher levels of indole acetic acid, non enzymatic antioxidants (phenolics and flavonoids) and antioxidant enzymes (catalase, ascorbte peroxidase, peroxidase, superoxide dismutase). As a result, ROS accumulation and electrolyte leakage were brought back to normal levels as observed in control seedlings. Hence, the rhizobacterium associated host seedlings achieved higher net assimilation (127.7%) and relative growth rate (113.5%) under 100 ppm of arsenate stress. The work concluded that P. conspicua alleviated arsenate stress in the host plants by imposing physical barrier as well as improving host seedlings' physiology and biochemistry.